THE EFFECT OF HALOTHANE INHALATIONAL ANAESTHETICS ON LIVER CELLS(Experimental Study of Halothane Toxic Effect in BALB C Mice) - Diponegoro University | Institutional Repository (UNDIP-IR)

CHAPTER 3
THEORITICAL FRAMEWORK, CONCEPTUAL FRAMEWORK
AND HYPOTHESIS

3.1 Theoretical Framework

Halothane
Induction of
Cytochrom P450
(CYP2E1 and 2A6)

Induction of
Cytochrom P450 (2A6
and 3A4)
Hypoxia

Hepatotoxic metabolites
(bromide and fluoride ions,
other volatil metabolites)

Genotypic and

phenotypic variability
in metabolism,
cytoprotection and
immune response

Increasing Trifluroacetylacid production
binding

Liver protein

Trifluroacetylated
protein

Specific
antibody

Liver cells damage
(nucleus changes: enlarge,
karyorhexis, karyolysis)

Altered liver
antigens

Liver cells necrosis
Moderate / Severe

Liver cells
necrosis
Mild / Severe

Figure 2. Theoretical Framework of the study

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3.2 Conceptual Framework
Independent Variable

Dependent Variable
Liver cells nucleus changes
(enlarge, karyorhexis,

Halothane
Cytochrome P450 2E1
changes
Figure 3. Conceptual Framework of the study

3.3 Hypothesis
3.3.1

There is an association between the time of halothane exposure with
severity of liver cells nucleus changes

3.3.2

Halothane exposure can induce changes of cytochrome P450 in the
liver

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