VARIASI GENETIK DENGAN TEKNIK RAPD PADA MUTAN PISANG KEPOK YAING TAHAN TERHADAP PENYAKIT BDB HASIL MUTASI INDUKSI DENGAN EMS.
i
'i, -;. F " -i lwA !.i"-r a d..P.tu"
:ri..q!MF;idtuio.tu
s
hud. iinu!:l nubtd
Bifuldmdor6alEEcddvo(@r'
hy EMs
\r€ sLrd
5
Niig 4 Fimd (0PM1, 0P^01 oPA@ tu oProrl tw sd€d m td, hd
FrFoprt tu' d low €htiE (3,7slt. B,sd on FlynoDhi. b&r,r rslile l]'d
by
od
xq6*: dtiw
xe@r
o!tu": M:
by
Dd@1;
6)
akr 11 tu
tuPD
edsry
3$,
er
i8
BDB
yq rdrm di*&bh
rdedi@ 3{@tud (sdolo, or,2@) tu B@tu !s{
olcr
ffi@
orb.
200e) !.rh n6uiju&e hair Fs opdnd Bah@ddii 0994) md@ko lcis
id e udw Pbry ar jds rk y@s
h4ad
Mds!rth , 41 0@t rrd 4rtu nhi dfri F& piss r.!or. !t!
*Ei de dll' deip ErvrM.'iec surphoi& (EMs). Mdudr Mj.k 1re6), EMs
D4FbbbFoifuFdaDN^fub'al@dlggfuein{galon
rbd@ Pqdih *jbis dilbjurls ohh tuyuni! r 4112@t,.]Mp Lddw
piss
Eja
d
tu pisg r@r.
ebebrtutesk
d
Ft
huirnyr diFoLeh
ri
y4tu,.hdq @d,
b3u4 rbir
nub nj, mh dq
5
BDB
, 4i C@t) !e Etu
pMigEJFUp4kFd!!eFndud.odd'tn'vq4'
@ddosi sdojfty4 Ir!@ d dr c@) nd,Fb bd$ Db pios Ldi{
gs3lma4codd4qFlitlfu!fui
e.e oo6lei
tuiai @d!
y@,
erui*i
F,u Etur Dhdd. Hdr tsEritu Yed
'*Pi
'i4r
l mdsdhui @brt6 $idk FB
D.D@ dkdtuya dd sld ntu u@ tus tuja $d tu
*idin:
rdldi Fir nub.
tss k@k dd 5 id:vidu riss nja s
FnbdgFFd'bibldukd'irtriqldFFndW4u6'
rutur@hhirs&rd(500N
r,5% sDs ($^).0.33 @ Nadu dffi sdp ro d ffir€rr*i) P,i! tu
f,si d.@ ccl;r€e 5310 (4@ 4bi 20
n.ijr). srymtu d@M d{s@ *obr e6% d{F Fbd4a i : ,. Eid,@
rns.Hcr, pts 7,rr 0,r nM EDTA, pH 3),
DNA d ddrD {fu hc ffiq 00
$r-jsp hho$a NAE 6 dpEsipihi Lsb.ri d{@ tu01 d6.rur di4ii,
rdnrmtuNiddor0rcr@d
DNA dilji dh@ i.bd. rltkhrcis Fi! sr 4m$ 0,3% ie Fi.ndr
nisi ('@tei! (0,r% ho@ph@r brw (,r) + 4fn tuF (,r). (dk DNr
ftoFhpds.midNcionr]BK
-w rM2 (e!bd ,6 de r0 @).
K@[d DxA (Fs'brl . dhi ABsd l 50 4hl x brd FiFe@, J0 ae/d
4@Frsidldkoiv4id&ind'&
d@ Abedrdi.D
&&do i6il bqi dh
^b$rtuno
P{di'ehM4ui*0'0Pfu4ydfu
lolum.*hn6Pu@ditu
glclpH3,],sdKcl',dMgcl!
0990)
o.@rei
sdtu(rhj,
r00 FM
ndiig naiis
d.A'Ir. dT1P, dcTP, dcrP (Poresal 5
p*o4dF6d'I4DNA@nfu1dlT4FlbeccDNAP(E5@Pli6ki
F@j44 @d tuil mprinbi duji tu DN^ lrfu r D Dxl /4rr',
(si@ UsA) d.iF Dd! dtubftsn DNA odr 3.1 r,6% @*. x.nudi& 3d
di@dedu0,5lg/in&hjdiu&o
(e4'ci I Ddn,
tut &rsid
orlir6i 4imr4 O6.e"ci 1 edr, &4i@ Otci , edo
(?}c;5 n{i) suu d
6
,,,P,&dbpd!6id@eobcMhahr
M6b k).
6.,h.{d'keddudim(!4@dw6rq@G4,
'k
suF! DNA bsir drlifksi sborFr I rr dd dimq d4o 1d /odiB .i{
pJ.rl!4$P44'4d're\MF660trNlntsjl"lu
r&d4 4€.
c€r
hdr
&hbq; *rej
e
d dtu6Ei8 dnb e dPtui
EdlelErfu.d4bTrqDNA
FlFqadeDlG.Ei'Fd9]i@!*i@9lifuircRdFIfuq{ede
Find to@.l. ss dde tu hdsb 6e !j@g dd DNA col ru
'
p!@ P.R,
&!@ dog didi
dcD !b! ddnP). sdm. dertoh
dcJp abu dnP) di@d
@ d$rsii!&l{rid.
@rida
y4g
(dAIP, dcTA
$!d ld n, d.n.
ou4l'ebhaccDF6dtudfusdDN^yog&miFjElHgd'
.ni!' ks,i se skutr sB
NcDtGNBar
d h' llr\rliidqrjllq
'dhld+seLuNLdj&pdaddofu! Eh€E sdojubF dit!|$o
&i'risis
!d
oudpi4(@kds@Ffurors
pih w@ hwlrmt (6mbu D Ho
d{p pnEe oPBor. sdujuhF &@ ic4gtu Ftu 0PB6 pd! uotr Etu
Freo 0,r% EMs 2 jm &tr kloi nuui Fu*tr 0,5% EMs - 5 j@rtud
d.i !
rira (clnbd
]) Pd! roD nlh
&F*kdpfolilpibhd|MrliikcjDN
, oPDm
tun sfh hliyr
l
de
pnms (oPAc, orDr1, oPtsot
oPH07).
6
$drighr F& pnbr
oPArt ya3
renF.rirab
pib.
sibi d
d (toe) tur@lon blbu dtu itu Fdi cddrE tu d m ru
litjtdilmDdinolfg.64mdDNA'
sd d.@ msun
k6 3 pim dii6il@ r@ hal Dib crsbcl D
jtu
M6li@ jubl.l' bel b&Fr Etbod t6pi pi' 4 Fr@e hF eib
D@4utu ptur orMr, oPA@, oPAoe dh oPs07 dd$ jlbrrn dar y6s iddif
d&n Firu 6 pi! (3,7ryr. Ds&sb FE@ jurar, ei! Fg Flimdr dli
etu
pi@s tuja
6pdFbghhlfu&pddlny.M
ddrdd 6dr buM rimr tuir s.Fh
Gmb[1'r{llm!li6l4it'6'd4@euruDxA!i@K4otd4mMggu*&
pfuo!e'duol$.M-l(bNAldq,=UoDlfufu
(kotur) j 2 = u@ otu Frtu 02% EMs - 2 jm: 3 = krm nu@
pd.run0,r%EMs.Jj :1-uonntutsnrrund0J%EMs,2j4
Db4fuF6khNabdB$wilr'dBf4dUdl6ibt4
ftolfu
nn d.i@ FE|Blta
mdk
Fda
nud piss k+L hd Fturu 0J% EMs - 2
scsr3 ldg n 4sdo tr ?64 drtsl d id &n
enerik r:da klo!
@b
3e6
4. Pi& lrv!ry 30
subla.
(sFr
DNA
Q'q rs i!5 rii* nwd t e ya
sbla. (md DNA nM). etu!tu rid.k nu.ul k
nu6) sddjuhF Fe
Fd. o,.ry
13dqdd Fdki hciu F& DN^ Elh bjdimd.
r@btu sd De huh tq ero h.Np
30r
dr
(t 4
nunol b.bdF be
inekr hlB i.ldits
prd{ skutr DNA noMh Mw Dlbsi rid
'6jdi
Fdbtu rib pda trturid{. Msud xDn d Biftr osto) EMs reruFb
{gd F4dkjlsi pg nsrbbh @brbd skm iurl{ridl s.lei!!ry! seNi , d
(2007) n{F,*o Me Fmrds 6 hihFF pb pd ft4tr DNA d nlbr
tud,&olda,rrc@d.)bs M$i sild sim nnFkm hsil tsroisho scdtN
DN^ (Pdtu, Mtssisi .bu dd6D
'id.k
pe
fubzEdl@p|ifika]bh!d+@p!fl
olhpigigKrytdspnsggfuPhgoP^@fuoPl@,M=l
(b DNA Lddq r= !d butri nM (koihl) : 2 . kioi nub Fdslu
0r% EMs
2
im: I
nub Frhud
DtmroikliFdsedwxdofulBKs
j
Ird nub Fl.lu
Frho
= u@ Dub
0r% trMe - 2 jm: d6 r =
0J% EMs .
5
r4=u@
0,t% EMS
cob r. lxia;nrlifibi dd@ ft!
ih"-r ,, = b'."6 j@*'tu o.
dhlM oryd Ns . 5 1= {o nub ldtu
:h5=u.inMFrtuo,s%m 5jM
0,5%
Ns ljD:
m'1i6|dlghad@.d!umDNA
Di@ikriFes4bfNNodBKSwibF|&4diUnisEibRjq
Mhr'Fnduldi,ehel"sc{L3.dU@muBnesskFrfui
ii---.=roxoav^.cm,=roro
w$|B4diUt6]ds,
lkonbr) diFl dnidp'tku trMei enkd:
nru feo![] rdci g{dir d!@ jwLd
dbpdinod scblilr 6 rib G.Btt dd 160 t6 ]og h!tuur&ipi 0 oin{
r
,id bidi
i
pd!
'D&t
(rr{a
|
rcnpret' (!d hyc AIL!.Pdo(A.
6*E Psql! rtu\ t{daqiq)
.*;,. i--;";,,r,-"
tulltanan
at
lpr
bku nlLliirli
) in
ridoftsh
G.Nt Dn .ir' b
HiFd)n Per'NsPt$
IJs^
ttu
rir?
lyJn'e64|4do'lPz4^pcfu4!da
sdqreeo
Ao
c
trB.
aDa R s.
^bba
aula.
R
r.! ie(pptj4)
a@ne qh4sodEDu
t
,ptut' t
4rc
R{inhc ui4 UPD
y.,
.Fo, &i
s0(3)l
st,11l1) 21621'
sia- s. 16.
s$ihd xs od MFA Drc \'s,d B.
ih.ra i'r.
nok€E.
&"
hddsi
o
iM!r'r"40r",
dd!o
si p!& pi$g @tu bnu
_o ef
or
'"F,hi)06|]v.fu'.iphoJ"'l'
fed;d*,.
"i
-
r
od.rd
a6
hs
srari &oru @
2o0?
r@ Ed4{ Edt
void nqror4i ridd
Nr
waldBg{'djUln€ads!{
Pisss tuja
'i, -;. F " -i lwA !.i"-r a d..P.tu"
:ri..q!MF;idtuio.tu
s
hud. iinu!:l nubtd
Bifuldmdor6alEEcddvo(@r'
hy EMs
\r€ sLrd
5
Niig 4 Fimd (0PM1, 0P^01 oPA@ tu oProrl tw sd€d m td, hd
FrFoprt tu' d low €htiE (3,7slt. B,sd on FlynoDhi. b&r,r rslile l]'d
by
od
xq6*: dtiw
xe@r
o!tu": M:
by
Dd@1;
6)
akr 11 tu
tuPD
edsry
3$,
er
i8
BDB
yq rdrm di*&bh
rdedi@ 3{@tud (sdolo, or,2@) tu B@tu !s{
olcr
ffi@
orb.
200e) !.rh n6uiju&e hair Fs opdnd Bah@ddii 0994) md@ko lcis
id e udw Pbry ar jds rk y@s
h4ad
Mds!rth , 41 0@t rrd 4rtu nhi dfri F& piss r.!or. !t!
*Ei de dll' deip ErvrM.'iec surphoi& (EMs). Mdudr Mj.k 1re6), EMs
D4FbbbFoifuFdaDN^fub'al@dlggfuein{galon
rbd@ Pqdih *jbis dilbjurls ohh tuyuni! r 4112@t,.]Mp Lddw
piss
Eja
d
tu pisg r@r.
ebebrtutesk
d
Ft
huirnyr diFoLeh
ri
y4tu,.hdq @d,
b3u4 rbir
nub nj, mh dq
5
BDB
, 4i C@t) !e Etu
pMigEJFUp4kFd!!eFndud.odd'tn'vq4'
@ddosi sdojfty4 Ir!@ d dr c@) nd,Fb bd$ Db pios Ldi{
gs3lma4codd4qFlitlfu!fui
e.e oo6lei
tuiai @d!
y@,
erui*i
F,u Etur Dhdd. Hdr tsEritu Yed
'*Pi
'i4r
l mdsdhui @brt6 $idk FB
D.D@ dkdtuya dd sld ntu u@ tus tuja $d tu
*idin:
rdldi Fir nub.
tss k@k dd 5 id:vidu riss nja s
FnbdgFFd'bibldukd'irtriqldFFndW4u6'
rutur@hhirs&rd(500N
r,5% sDs ($^).0.33 @ Nadu dffi sdp ro d ffir€rr*i) P,i! tu
f,si d.@ ccl;r€e 5310 (4@ 4bi 20
n.ijr). srymtu d@M d{s@ *obr e6% d{F Fbd4a i : ,. Eid,@
rns.Hcr, pts 7,rr 0,r nM EDTA, pH 3),
DNA d ddrD {fu hc ffiq 00
$r-jsp hho$a NAE 6 dpEsipihi Lsb.ri d{@ tu01 d6.rur di4ii,
rdnrmtuNiddor0rcr@d
DNA dilji dh@ i.bd. rltkhrcis Fi! sr 4m$ 0,3% ie Fi.ndr
nisi ('@tei! (0,r% ho@ph@r brw (,r) + 4fn tuF (,r). (dk DNr
ftoFhpds.midNcionr]BK
-w rM2 (e!bd ,6 de r0 @).
K@[d DxA (Fs'brl . dhi ABsd l 50 4hl x brd FiFe@, J0 ae/d
4@Frsidldkoiv4id&ind'&
d@ Abedrdi.D
&&do i6il bqi dh
^b$rtuno
P{di'ehM4ui*0'0Pfu4ydfu
lolum.*hn6Pu@ditu
glclpH3,],sdKcl',dMgcl!
0990)
o.@rei
sdtu(rhj,
r00 FM
ndiig naiis
d.A'Ir. dT1P, dcTP, dcrP (Poresal 5
p*o4dF6d'I4DNA@nfu1dlT4FlbeccDNAP(E5@Pli6ki
F@j44 @d tuil mprinbi duji tu DN^ lrfu r D Dxl /4rr',
(si@ UsA) d.iF Dd! dtubftsn DNA odr 3.1 r,6% @*. x.nudi& 3d
di@dedu0,5lg/in&hjdiu&o
(e4'ci I Ddn,
tut &rsid
orlir6i 4imr4 O6.e"ci 1 edr, &4i@ Otci , edo
(?}c;5 n{i) suu d
6
,,,P,&dbpd!6id@eobcMhahr
M6b k).
6.,h.{d'keddudim(!4@dw6rq@G4,
'k
suF! DNA bsir drlifksi sborFr I rr dd dimq d4o 1d /odiB .i{
pJ.rl!4$P44'4d're\MF660trNlntsjl"lu
r&d4 4€.
c€r
hdr
&hbq; *rej
e
d dtu6Ei8 dnb e dPtui
EdlelErfu.d4bTrqDNA
FlFqadeDlG.Ei'Fd9]i@!*i@9lifuircRdFIfuq{ede
Find to@.l. ss dde tu hdsb 6e !j@g dd DNA col ru
'
p!@ P.R,
&!@ dog didi
dcD !b! ddnP). sdm. dertoh
dcJp abu dnP) di@d
@ d$rsii!&l{rid.
@rida
y4g
(dAIP, dcTA
$!d ld n, d.n.
ou4l'ebhaccDF6dtudfusdDN^yog&miFjElHgd'
.ni!' ks,i se skutr sB
NcDtGNBar
d h' llr\rliidqrjllq
'dhld+seLuNLdj&pdaddofu! Eh€E sdojubF dit!|$o
&i'risis
!d
oudpi4(@kds@Ffurors
pih w@ hwlrmt (6mbu D Ho
d{p pnEe oPBor. sdujuhF &@ ic4gtu Ftu 0PB6 pd! uotr Etu
Freo 0,r% EMs 2 jm &tr kloi nuui Fu*tr 0,5% EMs - 5 j@rtud
d.i !
rira (clnbd
]) Pd! roD nlh
&F*kdpfolilpibhd|MrliikcjDN
, oPDm
tun sfh hliyr
l
de
pnms (oPAc, orDr1, oPtsot
oPH07).
6
$drighr F& pnbr
oPArt ya3
renF.rirab
pib.
sibi d
d (toe) tur@lon blbu dtu itu Fdi cddrE tu d m ru
litjtdilmDdinolfg.64mdDNA'
sd d.@ msun
k6 3 pim dii6il@ r@ hal Dib crsbcl D
jtu
M6li@ jubl.l' bel b&Fr Etbod t6pi pi' 4 Fr@e hF eib
D@4utu ptur orMr, oPA@, oPAoe dh oPs07 dd$ jlbrrn dar y6s iddif
d&n Firu 6 pi! (3,7ryr. Ds&sb FE@ jurar, ei! Fg Flimdr dli
etu
pi@s tuja
6pdFbghhlfu&pddlny.M
ddrdd 6dr buM rimr tuir s.Fh
Gmb[1'r{llm!li6l4it'6'd4@euruDxA!i@K4otd4mMggu*&
pfuo!e'duol$.M-l(bNAldq,=UoDlfufu
(kotur) j 2 = u@ otu Frtu 02% EMs - 2 jm: 3 = krm nu@
pd.run0,r%EMs.Jj :1-uonntutsnrrund0J%EMs,2j4
Db4fuF6khNabdB$wilr'dBf4dUdl6ibt4
ftolfu
nn d.i@ FE|Blta
mdk
Fda
nud piss k+L hd Fturu 0J% EMs - 2
scsr3 ldg n 4sdo tr ?64 drtsl d id &n
enerik r:da klo!
@b
3e6
4. Pi& lrv!ry 30
subla.
(sFr
DNA
Q'q rs i!5 rii* nwd t e ya
sbla. (md DNA nM). etu!tu rid.k nu.ul k
nu6) sddjuhF Fe
Fd. o,.ry
13dqdd Fdki hciu F& DN^ Elh bjdimd.
r@btu sd De huh tq ero h.Np
30r
dr
(t 4
nunol b.bdF be
inekr hlB i.ldits
prd{ skutr DNA noMh Mw Dlbsi rid
'6jdi
Fdbtu rib pda trturid{. Msud xDn d Biftr osto) EMs reruFb
{gd F4dkjlsi pg nsrbbh @brbd skm iurl{ridl s.lei!!ry! seNi , d
(2007) n{F,*o Me Fmrds 6 hihFF pb pd ft4tr DNA d nlbr
tud,&olda,rrc@d.)bs M$i sild sim nnFkm hsil tsroisho scdtN
DN^ (Pdtu, Mtssisi .bu dd6D
'id.k
pe
fubzEdl@p|ifika]bh!d+@p!fl
olhpigigKrytdspnsggfuPhgoP^@fuoPl@,M=l
(b DNA Lddq r= !d butri nM (koihl) : 2 . kioi nub Fdslu
0r% EMs
2
im: I
nub Frhud
DtmroikliFdsedwxdofulBKs
j
Ird nub Fl.lu
Frho
= u@ Dub
0r% trMe - 2 jm: d6 r =
0J% EMs .
5
r4=u@
0,t% EMS
cob r. lxia;nrlifibi dd@ ft!
ih"-r ,, = b'."6 j@*'tu o.
dhlM oryd Ns . 5 1= {o nub ldtu
:h5=u.inMFrtuo,s%m 5jM
0,5%
Ns ljD:
m'1i6|dlghad@.d!umDNA
Di@ikriFes4bfNNodBKSwibF|&4diUnisEibRjq
Mhr'Fnduldi,ehel"sc{L3.dU@muBnesskFrfui
ii---.=roxoav^.cm,=roro
w$|B4diUt6]ds,
lkonbr) diFl dnidp'tku trMei enkd:
nru feo![] rdci g{dir d!@ jwLd
dbpdinod scblilr 6 rib G.Btt dd 160 t6 ]og h!tuur&ipi 0 oin{
r
,id bidi
i
pd!
'D&t
(rr{a
|
rcnpret' (!d hyc AIL!.Pdo(A.
6*E Psql! rtu\ t{daqiq)
.*;,. i--;";,,r,-"
tulltanan
at
lpr
bku nlLliirli
) in
ridoftsh
G.Nt Dn .ir' b
HiFd)n Per'NsPt$
IJs^
ttu
rir?
lyJn'e64|4do'lPz4^pcfu4!da
sdqreeo
Ao
c
trB.
aDa R s.
^bba
aula.
R
r.! ie(pptj4)
a@ne qh4sodEDu
t
,ptut' t
4rc
R{inhc ui4 UPD
y.,
.Fo, &i
s0(3)l
st,11l1) 21621'
sia- s. 16.
s$ihd xs od MFA Drc \'s,d B.
ih.ra i'r.
nok€E.
&"
hddsi
o
iM!r'r"40r",
dd!o
si p!& pi$g @tu bnu
_o ef
or
'"F,hi)06|]v.fu'.iphoJ"'l'
fed;d*,.
"i
-
r
od.rd
a6
hs
srari &oru @
2o0?
r@ Ed4{ Edt
void nqror4i ridd
Nr
waldBg{'djUln€ads!{
Pisss tuja