Uji Efek Repellent Nabati Ekstrak Bawang Putih (Allium sativum L.) Terhadap Tikus Putih Jantan Galur Wistar

LAMPIRAN

Lampiran1. Surat hasil identifikasi tumbuhan

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Lampiran 2.Gambar umbi bawang putih (Allium sativum L.)

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Lampiran 3. Gambar Hewan uji tikus putih jantan

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Lampiran 4. Surat komisi etik

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Lampiran 5. Bagan penelitian

Bawang Putih
Dikupas
Ditimbang
Dicuci, ditiriskan
Dimasukkan ke dalam blender,
ditambahkan aquades 1000 mL,
kemudian di blender sampai halus dan
tercampur seluruhnya
Masukkan ke dalam wadah tutup
terlalu diamkan selama 24 jam
Skrining Fitokimia
Pemeriksaan
alkaloida
Pemeriksaan
flavonoida

Disaring dengan kain flanel

Dicukupkan dengan aquades
hingga 1000 mL

Ekstrak air bawang putih

Pemeriksaan
saponin
Pemeriksaan
tanin

Karakterisasi
bawang putih

Aktivitas sebagai repellent
nabati terhadap tikus

Pemeriksaan
glikosida
antrakuinon

Hasil

Pemeriksaan
steroida/
triterpenoida

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Lampiran 6. Data hasil pengujian ekstrak air bawang putih

Jumlah sisa makanan tikus pada saat pengujian ekstrak air bawang putih
Perlakuan

Hari

EABP
100g/L

1

Sisa makanan tikus (gram)
I
II
III
10 g
8g
5g

2

5g

3g

-

2 hari

2 hari

1 hari

Efek Repellent3

Perlakuan

hari

EABP 200g/L

1

Sisa makanan tikus (gram)
I
II
III
15 g
18 g
19 g

2

9g

11 g

17 g

3

4g

6g

9g

4

-

-

3g

5

-

-

-

3 hari

3 hari

4 hari

Efek Repellent

Perlakuan
EABP 400g/L

Hari

Sisa makanan tikus (gram)
I

II

III

1

35 g

33 g

28 g

2

26 g

20 g

19 g

3

20 g

15 g

10 g

4

15 g

7g

4g

5

9g

0g

0g

6

0g

-

-

5 hari

4 hari

4 hari

Efek Repellent

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Perlakuan

Hari

EABP 800g/L

1

Sisa makanan tikus (gram)
I
II
III
50 g
48 g
47 g

2

47 g

35 g

38 g

3

35 g

27 g

25 g

4

29 g

18 g

16 g

5

19 g
7 hari

11 g
6 hari

9g
6 hari

Efek Repellent

Perlakuan
EABP
1600g/L

Hari

Sisa makanan tikus (gram)

1

I
60

II
64

III
62

2

54

57

56

3

50

49

48

4

46

41

40

5

39

38

35

6

31

33

28

7

24

29

21

8

15

21

17

9

8

17

11

10

-

6

4

11

-

-

-

9 hari

10 hari

10 hari

Efek Repellent

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Perlakuan

Hari

Kontrol Negatif

Efek Repellent

Ulangan
II
8

1

I
5

III
0

2

0

5

5

3

7

4

0

4

0

0

0

5

0

0

5

6

0

6

5

7

0

0

0

8

5

5

0

9

4

0

6

10

0

0

0

11

0

0

0

-

-

-

-

Data hasil pengujian efek repellent EABP terhadap jumlah sisa makanan tikus
(gram)
Hari
1
2
3
4
5
6
7
8
9
10
11
Efek
Repellent

Kontrol (-)
4,3
0
3,6
0
1,6
3,6
0
3,3
3,3
0
0
-

I
7,6
2,6
0
2 hari

Perlakuan
II
III
17,3
32
12,3
21,6
6,3
15
1
8,6
0
3
0
4 hari
5 hari

IV
48,3
40
29
21
13
6,3
1,3
0
7 hari

V
62
55,6
49
42,3
37,3
30,6
24,6
17,6
12
3,3
0
10 hari

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Lampiran 7. Hasil variansi ANOVA
ANOVA
Sum of Squares
Hari1

Between Groups

5

1602.322

92.667

12

7.722

Total

8104.278

17

Between Groups

7226.278

5

1445.256

158.667

12

13.222

Total

7384.944

17

Between Groups

5257.167

5

1051.433

145.333

12

12.111

Total

5402.500

17

Between Groups

4263.167

5

852.633

189.333

12

15.778

Total

4452.500

17

Between Groups

3211.167

5

642.233

135.333

12

11.278

Total

3346.500

17

Between Groups

2155.111

5

431.022

54.000

12

4.500

Total

2209.111

17

Between Groups

1492.667

5

298.533

43.333

12

3.611

1536.000

17

749.167

5

149.833

35.333

12

2.944

Total

784.500

17

Between Groups

347.778

5

69.556

60.667

12

5.056

408.444

17

Between Groups

27.778

5

5.556

Within Groups

18.667

12

1.556

Total

46.444

17

Between Groups

.000

5

.000

Within Groups

.000

12

.000

Total

.000

17

Within Groups

Hari3

Within Groups

Hari4

Within Groups

Hari5

Within Groups

Hari6

Within Groups

Hari7

Within Groups
Total
Hari8

Between Groups
Within Groups

Hari9

Within Groups
Total
Hari10

Hari11

Mean Square

8011.611

Within Groups

Hari2

df

F

Sig.

207.495

.000

109.305

.000

86.816

.000

54.040

.000

56.947

.000

95.783

.000

82.671

.000

50.887

.000

13.758

.000

3.571

.033

.

.

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Lampiran 8. Hasil uji Tukey HSD
Hari1
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

Kontrol negatif

3

4.3333

EABP 100 g/L

3

7.6667

EABP 200 g/L

3

EABP 400 g/L

3

EABP 800 g/L

3

EABP 1600 g/L

3

3

4

5

17.3333
32.0000
48.3333
62.0000

Sig.

.688

1.000

1.000

1.000

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.

Hari2
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

Kontrol negatif

3

.0000

EABP 100 g/L

3

2.6667

EABP 200 g/L

3

EABP 400 g/L

3

EABP 800 g/L

3

EABP 1600 g/L

3

Sig.

3

4

5

2.6667
12.3333

12.3333
21.6667
40.0000
55.6667

.940

.059

.072

1.000

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.

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Hari3
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

EABP 100 g/L

3

.0000

Kontrol negatif

3

3.6667

EABP 200 g/L

3

6.3333

EABP 400 g/L

3

EABP 800 g/L

3

EABP 1600 g/L

3

3

4

6.3333
15.0000
29.0000
49.0000

Sig.

.293

.083

1.000

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Hari4
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

EABP 100 g/L

3

.0000

Kontrol negatif

3

.0000

EABP 200 g/L

3

1.0000

EABP 400 g/L

3

8.6667

EABP 800 g/L

3

EABP 1600 g/L

3

Sig.

3

21.0000
42.3333
.153

1.000

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.

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Hari5
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

EABP 100 g/L

3

.0000

EABP 200 g/L

3

.0000

Kontrol negatif

3

1.6667

EABP 400 g/L

3

3.0000

EABP 800 g/L

3

EABP 1600 g/L

3

3

13.0000
37.3333

Sig.

.875

1.000

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Hari6
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

EABP 100 g/L

3

.0000

EABP 200 g/L

3

.0000

EABP 400 g/L

3

.0000

Kontrol negatif

3

3.6667

EABP 800 g/L

3

EABP 1600 g/L

3

Sig.

3

3.6667
6.3333
30.6667

.341

.648

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.

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Hari7
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

EABP 100 g/L

3

.0000

EABP 200 g/L

3

.0000

EABP 400 g/L

3

.0000

Kontrol negatif

3

.0000

EABP 800 g/L

3

1.3333

EABP 1600 g/L

3

24.6667

Sig.

.949

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Hari8
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

EABP 100 g/L

3

.0000

EABP 200 g/L

3

.0000

EABP 400 g/L

3

.0000

EABP 800 g/L

3

.0000

Kontrol negatif

3

3.3333

EABP 1600 g/L

3

Sig.

17.6667
.237

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.

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Hari9
a

Tukey HSD
Perlakuan

Subset for alpha = 0.05
N

1

2

EABP 100 g/L

3

.0000

EABP 200 g/L

3

.0000

EABP 400 g/L

3

.0000

EABP 800 g/L

3

.0000

Kontrol negatif

3

3.3333

EABP 1600 g/L

3

Sig.

12.0000
.491

1.000

Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
Hari10
a

Tukey HSD
Perlakuan

Subset for alpha =
0.05
N

1

EABP 100 g/L

3

.0000

EABP 200 g/L

3

.0000

EABP 400 g/L

3

.0000

EABP 800 g/L

3

.0000

Kontrol negatif

3

.0000

EABP 1600 g/L

3

3.3333

Sig.

.058

Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 3.000.

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