BIODEGRADASI OIL SLUDGE DENGAN VARIASI LAMA WAKTU INKUBASI DAN JENIS KONSORSIUM BAKTERI YANG DIISOLASI DARI LUMPUR PANTAI KENJERAN Repository - UNAIR REPOSITORY

  

LAMPIRAN

1. Data hasil penelitian A. Data Standart Plate Count (SPC)

  21

  18 M1 14 >3 x 10

  27

  (318 x 10

  25

  )

  19 M2 2 x 10

  11

  20 M2 2,7 x 10

  11

  21 M2 2,9 x 10

  11

  22 M2 7 2 x 10

  22

  23 M2 7 9,1 x 10

  24 M2 7 2,2 x 10

  17 M1 14 9,4 x 10

  27 M2 14 1,5 x 10

  30 M3 1,1 x 10

  9

  29 M3 7,3 x 10

  9

  28 M3 4,7 x 10

  27

  )

  20

  25

  (302 x 10

  25

  26 M2 14 >3 x 10

  25

  25 M2 14 1,2 x 10

  24

  26

  No. formula waktu inkubasi hasil SPC

  5 K 7 3,1 x 10

  8 K 14 3,2 x 10

  23

  7 K 14 5,2 x 10

  15

  6 K 7 7,1 x 10

  16

  21

  9 K 14 3,5 x 10

  4 K 7 1,2 x 10

  6

  3 K 5,5 x 10

  2 K 1,1 x 10 5

  6

  1 K 7 x 10

  22

  24

  16 M1 14 2,2 x 10

  11

  21

  15 M1 7 9,8 x 10

  22

  14 M1 7 1 x 10

  20

  13 M1 7 2,2 x 10

  12 M1 1,7 x 10

  10 M1 >3,0 x 10

  11

  11 M1 1,7 x 10

  )

  9

  (347 x 10

  11

  11 ADLN Perpustakaan Universitas Airlangga

  31 M3 7 1,7 x 10

  20

  32 M3 7 2,7 x 10

  21

  33 M3 7 1,1 x 10

  20

  34 M3 14 2,3 x 10

  25

  35 M3 14 2,5 x 10

  25

  36 M3 14 2,1 x 10

  27 ADLN Perpustakaan Universitas Airlangga

  

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B. Data nilai oil sludge residu

C. Data oil sludge residu

  7 M2 0,15

  

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  14 M3

  14 M3 0,14

  14 M3 0,13

  7 M3 0,13

  7 M3 0,18

  7 M3 0,17

  14 M2 0,23 M3 0,21 M3 0,21 M3 0,17

  14 M2 0,14

  14 M2 0,14

  7 M2 0,13

  7 M2 0,16

  Residu SLUDGE WAKTU

  ISOLAT 0,31 K 0,32 K 0,32 K 0,28

  14 M1 0,14

  14 M1 0,14

  7 M1 0,15

  7 M1 0,23

  7 M1 0,22

  0,31 M1 0,31 M1 0,24

  14 K 0,3 M1

  14 K 0,25

  14 K 0,24

  7 K 0,22

  7 K 0,28

  7 K 0,29

  14 M1 0,27 M2 0,24 M2 0,25 M2 0,17

D. Data oil sludge yang telah terdegradasi

  7 M2 0,17

  

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  14 M3 0,18

  14 M3 0,19

  14 M3 0,19

  7 M3 0,14

  7 M3 0,15

  7 M3 0,15

  14 M2 0,18 M3 0,09 M3 0,11 M3 0,11

  14 M2 0,18

  14 M2 0,19

  7 M2 0,16

  WAKTU

  ISOLAT SLUDGE DEGRADASI K 0,01 K K

  14 M1 0,18 M2 0,05 M2 0,08 M2 0,07

  14 M1 0,18

  14 M1 0,17

  7 M1 0,09

  7 M1 0,1

  7 M1 0,08

  14 K 0,07 M1 0,02 M1 0,01 M1 0,01

  14 K 0,08

  14 K 0,1

  7 K 0,04

  7 K 0,03

  7 K 0,04

  7 M2 0,15

  

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E. Data pH, oil sludge sisa, oil sludge yang terdegradasi dan log TPC masing-masing konsorsium bakteri.

  Kontrol 0 hari 7 hari 14 hari Persentase degradasi (%)

12,5

  25 Log TPC

6,2 17,8 23,6

PH 7 6,1 6,7 Persentase residu (%) 100 87,5

  75 Konsorsium M1 0 hari 7 hari 14 hari Persentase degradasi (%)

3,12 28,12 56,25

Log TPC

11,3 21,8 25,6

PH

  7 6,6

  7 Persentase residu (%)

96,88 71,88 43,75

Konsorsium M2 0 hari 7 hari 14 hari Persentase degradasi (%)

  21,87 50 56,25 Log TPC

10,7 21,5 26,2

PH

  7 6,3 6,8 Persentase residu (%) 78,13 50 43,75 Konsorsium M3 0 hari 7 hari 14 hari

  Persentase degradasi (%)

31,25 46,87 59,37

Log TPC 10,2 20,6

  26 PH 7 6,1 6,9 Persentase residu (%)

68,75 53,13 40,63

2. Hasil uji statistik A. Hasil analisis statistik pada TPC

  Dependent Variable:TPC F df1 df2 Sig.

  

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  Brown-Forsythe 4.185 2 11.000 .045 a. Asymptotically F distributed.

  a df1 df2 Sig.

  TPC Statistic

  c. Hasil uji Brown-Forsythe

Robust Tests of Equality of Means

  a. Design: Intercept + FORMULA + waktu_inkubasi

  11 24 .003 Tests the null hypothesis that the error variance of the dependent variable is equal across groups.

  3.765

  b. Hasil uji homogenitas menggunakan Levene's Test Levene's Test of Equality of Error Variances a

  a. Hasil uji normalitas menggunakan One-Sample Kolmogorov-Smirnov Test One-Sample Kolmogorov-Smirnov Test

  Kolmogorov-Smirnov Z .574 1.206 1.318 Asymp. Sig. (2-tailed) .896 .109 .062 a. Test distribution is Normal.

  Most Extreme Differences Absolute .096 .201 .220 Positive .096 .174 .220 Negative -.096 -.201 -.220

  Mean 5.5000 18.4731 7.0000 Std. Deviation 3.50102 6.94053 5.79655

  a

  36 Normal Parameters

  36

  36

  FORMULA TPC waktu_inkubasi N

• FORMULA * waktu_inkubasi

d. Hasil uji Games-Howell

  Multiple Comparisons

  TPC Games-Howell (I) FORMU LA

  (J) FORMU LA

  Mean Difference (I-J) Std. Error Sig.

  95% Confidence Interval Lower Bound Upper Bound

  KH0 KH7 -11.59333 1.74304 .081 -25.8794 2.6928 KH14 -17.38333

  

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• .83353 .000 -22.2213 -12.5454
• .62779 .003 -20.6765 -10.4768
• .74803 .000 -23.8849 -14.9084
• .84579 .001 -20.2415 -10.4185
• .88367 .000 -25.2585 -14.9282
• .73204 .001 -18.8270 -9.8930
• .87857 .000 -24.9589 -14.7011
• .83353 .000 12.5454 22.2213
• .59859 .011 6.4324 18.0810
• .86624 .002 7.8200 17.9067
• .73083 .001 8.9277 17.8657
• .59859 .011 -18.0810 -6.4324
• .24012 .000 -12.2018 -8.6982

  M2H0 -4.52000 .86596 .072 -9.5620 .5220 M2H7 -15.33000

  M2H14 -20.09333

  M1H14 -19.39667

  M3H14 -19.83000

  KH7 KH0 11.59333 1.74304 .081 -2.6928 25.8794 KH14 -5.79000 1.74318 .343 -20.0736 8.4936 M1H0 6.46667 1.64369 .293 -10.5137 23.4470 M1H7 -3.98333 1.65469 .581 -20.5549 12.5882 M1H14 -7.80333 1.70395 .200 -22.9160 7.3093 M2H0 7.07333 1.75891 .234 -6.9409 21.0876 M2H7 -3.73667 1.74907 .652 -17.9156 10.4423 M2H14 -8.50000 1.76770 .158 -22.3774 5.3774 M3H0 7.60667 1.69632 .211 -7.6976 22.9110 M3H7 -2.76667 1.69699 .826 -18.0537 12.5204 M3H14 -8.23667 1.76516 .170 -22.1527 5.6794

  KH14 KH0 17.38333

  KH7 5.79000 1.74318 .343 -8.4936 20.0736 M1H0 12.25667

  M1H7 1.80667 .62818 .435 -3.2977 6.9110 M1H14 -2.01333 .74836 .437 -6.5044 2.4777 M2H0 12.86333

  M2H7 2.05333 .84608 .521 -2.8597 6.9664 M2H14 -2.71000 .88395 .327 -7.8763 2.4563 M3H0 13.39667

  M3H7 3.02333 .73238 .161 -1.4467 7.4934 M3H14 -2.44667 .87885 .403 -7.5768 2.6835

  M1H0 KH0 5.12667 .59818 .065 -.6931 10.9465 KH7 -6.46667 1.64369 .293 -23.4470 10.5137 KH14 -12.25667

  M1H0 -5.12667 .59818 .065 -10.9465 .6931 M1H7 -15.57667

  M3H0 -3.98667 .73049 .071 -8.4526 .4793 M3H7 -14.36000

• .47232 .004 -18.6865 -9.8535

• .61554 .017 -16.2144 -4.1922
• .66664 .009 -21.5385 -8.3949
• .44657 .009 -13.3585 -5.1081
• .65987 .009 -21.2010 -8.2057
• .62779 .003 10.4768 20.6765
• .24012 .000 8.6982 12.2018
• .50930 .047 -7.5582 -.0818
• .67061 .010 5.4602 16.6532
• .48317 .002 8.1447 15.0353
• .74803 .000 14.9084 23.8849
• .47232 .004 9.8535 18.6865
• .50930 .047 .0818 7.5582
• .78432 .001 10.0676 19.6857
• .63158 .000 11.7354 19.0846
• .63337 .017 1.3532 8.7201
• .86624 .002 -17.9067 -7.8200
• .67061 .010 -16.6532 -5.4602
• .78432 .001 -19.6857 -10.0676
• .87804 .003 -15.9121 -5.7079
• .91459 .001 -20.8859 -10.2608
• .76908 .005 -14.6519 -5.0281
• .90966 .001 -20.5919 -10.0281
• .84579 .001 10.4185 20.2415
• .61554 .017 4.1922 16.2144

  KH7 3.73667 1.74907 .652 -10.4423 17.9156 KH14 -2.05333 .84608 .521 -6.9664 2.8597

  M2H7 KH0 15.33000

  M3H14 -15.31000

  M3H0 .53333 .76761 .999 -4.2799 5.3465 M3H7 -9.84000

  M2H14 -15.57333

  M2H7 -10.81000

  M1H14 -14.87667

  M1H0 -.60667 .64298 .983 -6.9193 5.7059 M1H7 -11.05667

  KH7 -7.07333 1.75891 .234 -21.0876 6.9409 KH14 -12.86333

  M3H14 -.43333 .79822 1.000 -5.3709 4.5042 M2H0 KH0 4.52000 .86596 .072 -.5220 9.5620

  M3H7 5.03667

  M2H7 4.06667 .76199 .074 -.5422 8.6756 M2H14 -.69667 .80383 .994 -5.6869 4.2935 M3H0 15.41000

  M2H0 14.87667

  KH7 7.80333 1.70395 .200 -7.3093 22.9160 KH14 2.01333 .74836 .437 -2.4777 6.5044 M1H0 14.27000

  M1H7 3.82000

  M1H14 KH0 19.39667

  M3H7 1.21667 .48551 .520 -2.2546 4.6880 M3H14 -4.25333 .68682 .100 -10.0378 1.5311

  M2H7 .24667 .64435 1.000 -5.0452 5.5385 M2H14 -4.51667 .69333 .090 -10.3766 1.3433 M3H0 11.59000

  M2H0 11.05667

  M1H14 -3.82000

  KH7 3.98333 1.65469 .581 -12.5882 20.5549 KH14 -1.80667 .62818 .435 -6.9110 3.2977 M1H0 10.45000

  M1H7 KH0 15.57667

  M3H14 -14.70333

  M3H0 1.14000 .44402 .529 -2.9563 5.2363 M3H7 -9.23333

  M2H14 -14.96667

  M2H0 .60667 .64298 .983 -5.7059 6.9193 M2H7 -10.20333

  M1H14 -14.27000

  M1H7 -10.45000

  

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• .87804 .003 5.7079 15.9121

• .74477 .002 6.7462 15.9405
• .88367 .000 14.9282 25.2585
• .66664 .009 8.3949 21.5385
• .91459 .001 10.2608 20.8859
• .78753 .001 11.0992 21.1141
• .78897 .033 .7284 10.7383
• .73083 .001 -17.8657 -8.9277
• .48317 .002 -15.0353 -8.1447
• .63158 .000 -19.0846 -11.7354
• .74477 .002 -15.9405 -6.7462
• .78753 .001 -21.1141 -11.0992
• .61255 .001 -13.9288 -6.8179
• .78181 .001 -20.7945 -10.8922
• .73204 .001 9.8930 18.8270
• .44657 .009 5.1081 13.3585
• .63337 .017 -8.7201 -1.3532
• .76908 .005 5.0281 14.6519
• .78897 .033 -10.7383 -.7284
• .61255 .001 6.8179 13.9288
• .78326 .037 -10.4190 -.5210
• .87857 .000 14.7011 24.9589
• .65987 .009 8.2057 21.2010

  KH7 8.23667 1.76516 .170 -5.6794 22.1527 KH14 2.44667 .87885 .403 -2.6835 7.5768

  M3H14 KH0 19.83000

  M3H14 -5.47000

  M3H0 10.37333

  M2H7 -.97000 .74629 .937 -5.5672 3.6272 M2H14 -5.73333

  M2H0 9.84000

  M1H7 -1.21667 .48551 .520 -4.6880 2.2546 M1H14 -5.03667

  KH7 2.76667 1.69699 .826 -12.5204 18.0537 KH14 -3.02333 .73238 .161 -7.4934 1.4467 M1H0 9.23333

  M3H7 KH0 14.36000

  M3H14 -15.84333

  M3H7 -10.37333

  M2H14 -16.10667

  M1H14 -15.41000

  M2H0 -.53333 .76761 .999 -5.3465 4.2799 M2H7 -11.34333

  M1H0 -1.14000 .44402 .529 -5.2363 2.9563 M1H7 -11.59000

  KH7 -7.60667 1.69632 .211 -22.9110 7.6976 KH14 -13.39667

  M3H14 .26333 .92654 1.000 -5.1148 5.6414 M3H0 KH0 3.98667 .73049 .071 -.4793 8.4526

  M3H7 5.73333

  M2H7 4.76333 .89551 .068 -.4542 9.9809 M3H0 16.10667

  M1H7 4.51667 .69333 .090 -1.3433 10.3766 M1H14 .69667 .80383 .994 -4.2935 5.6869 M2H0 15.57333

  KH7 8.50000 1.76770 .158 -5.3774 22.3774 KH14 2.71000 .88395 .327 -2.4563 7.8763 M1H0 14.96667

  M2H14 KH0 20.09333

  M3H7 .97000 .74629 .937 -3.6272 5.5672 M3H14 -4.50000 .89048 .080 -9.6835 .6835

  M2H14 -4.76333 .89551 .068 -9.9809 .4542 M3H0 11.34333

  M1H7 -.24667 .64435 1.000 -5.5385 5.0452 M1H14 -4.06667 .76199 .074 -8.6756 .5422 M2H0 10.81000

  M1H0 10.20333

  

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  M1H0 14.70333

  M1H7 4.25333 .68682 .100 -1.5311 10.0378 M1H14 .43333 .79822 1.000 -4.5042 5.3709 M2H0 15.31000

• .90966 .001 10.0281 20.5919

  M2H7 4.50000 .89048 .080 -.6835 9.6835 M2H14 -.26333 .92654 1.000 -5.6414 5.1148 M3H0 15.84333

• .78181 .001 10.8922 20.7945
• .78326 .037 .5210 10.4190 *. The mean difference is significant at the 0.05 level.

  M3H7 5.47000

  

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HASIL GAMES_HOWELL TPC

  3

  11 S TS TS S TS TS S TS TS S S B.

  1

  Kolmogorov-Smirnov Z 1.318 .896 .574 Asymp. Sig. (2-tailed) .062 .399 .896 a. Test distribution is Normal.

  Most Extreme Differences Absolute .220 .149 .096 Positive .220 .149 .096 Negative -.220 -.093 -.096

  Mean 7.0000 .2158 5.5000 Std. Deviation 5.79655 .06478 3.50102

  a

  36 Normal Parameters

  36

  36

  DU FORMULA N

  waktu_inkubasi SLUDGE_RESI

  Hasil uji normalitas menggunakan One-Sample Kolmogorov-Smirnov Test One-Sample Kolmogorov-Smirnov Test

   Hasil analisis statistik pada Residu oil sludge a.

  

10 S TS TS S TS S S TS S S S

  4

  

9 TS TS S TS S S TS S S S S

  

8 S TS TS S TS TS S TS S S TS

  

7 S TS TS S TS TS S TS S TS TS

  2

  

5 S TS TS S S S TS TS S S TS

  

4 S TS TS S S S TS TS S TS TS

  

3 TS TS TS S S TS TS S TS S S

  

2 S TS TS TS TS S TS TS S TS TS

  

1 TS TS TS TS TS TS TS TS TS TS TS

  8 9 10 11 TS S TS S S TS S S TS S S

  7

  6

  

5

  

6 TS TS S TS S S S S TS S S

  

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b.

  Hasil uji homogenitas menggunakan Levene's Test a Levene's Test of Equality of Error Variances

  Dependent Variable:SLUDGE_RESIDU F df1 df2 Sig.

  1.243

  11 24 .314 Tests the null hypothesis that the error variance of the dependent variable is equal across groups.

  a. Design: Intercept + FORMULA + waktu_inkubasi + FORMULA

• waktu_inkubasi c.

  Hasil uji ANOVA 2 arah

Between-Subjects Factors

  Value Label N FORMULA 1 K

  9

  2 M1

  9

  3 M2

  9

  4 M3

  9 WAKTU

  1

  12

  2

  7

  12

  3

  14

  12 Tests of Between-Subjects Effects

  Dependent Variable:SLUDGE_RESIDU Type III Sum

Source of Squares df Mean Square F Sig.

a

  Corrected Model .145 11 .013 152.877 .000 Intercept 1.677 1 1.677 1.948E4 .000 FORMULA .071 9 .008 91.344 .000

waktu_inkubasi .000 . . .

  FORMULA * .000 . . . waktu_inkubasi Error .002

  24 8.611E-5 Total 1.824

  36 Corrected Total .147

  35

a. R Squared = ,986 (Adjusted R Squared = ,979)

  

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d.

  Hasil uji Duncan

SLUDGE_RESIDU

  Duncan Subset

  FORM ULA N

  1

  2

  3 M3 9 .1744 M2 9 .1833 M1 9 .2267 K 9 .2789 Sig. .053 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005.

SLUDGE_RESIDU

  Duncan Subset

  WAKT U N

  1

  2

  3

  14 12 .1625

  7 12 .2117 12 .2733 Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005.

SLUDGE_RESIDU

  Duncan Subset

  INTER AKSI N

  1

  2

  3

  4

  5

  6

  7 M314 3 .1333 M214 3 .1367 M114 3 .1433 M27 3 .1600 M37 3 .1733 M30 3 .2167 M17 3 .2300 .2300 K14 3 .2367 M20

  3 .2533

  K7

  3 .2833

  M10

  3 .3067

  K0

  3 .3167 Sig. .224 .091 .091 .388 1.000 1.000 .199 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005.

C. Hasil analisis statistik pada Oil sludge yang telah terdegradasi a.

  b.

  

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  a. Design: Intercept + FORMULA + WAKTU + FORMULA * WAKTU

  11 24 .314 Tests the null hypothesis that the error variance of the dependent variable is equal across groups.

  1.243

  Dependent Variable:SLUDGE_DEGRADASI F df1 df2 Sig.

  Hasil uji homogenitas menggunakan Levene's Test

Levene's Test of Equality of Error Variances

a

  Hasil uji normalitas menggunakan One-Sample Kolmogorov-Smirnov Test

One-Sample Kolmogorov-Smirnov Test

  FORMULA WAKTU SLUDGE_DEG

  Most Extreme Differences Absolute .170 .220 .149 Positive .170 .220 .093 Negative -.170 -.220 -.149 c.

  Mean 2.50 2.0000 .1042 Std. Deviation 1.134 .82808 .06478

  a

  36 Normal Parameters

  36

  36

  RADASI N

  Kolmogorov-Smirnov Z 1.022 1.318 .896 Asymp. Sig. (2-tailed) .247 .062 .399 a. Test distribution is Normal.

  Hasil uji ANOVA 2 arah

Between-Subjects Factors

  Type III Sum of Squares df Mean Square F Sig. Corrected Model .145

  

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  3 K 9 .0411 M1 9 .0933 M2 9 .1367 M3 9 .1456 Sig. 1.000 1.000 .053 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005.

  2

  1

  Subset

  Duncan FORM ULA N

  Hasil uji Duncan

  a. R Squared = ,986 (Adjusted R Squared = ,979) d.

  35

  36 Corrected Total .147

  .009 6 .002 17.473 .000 Error .002 24 8.611E-5 Total .538

  2 .037 429.774 .000 FORMULA * WAKTU

  11 .013 152.877 .000 Intercept .391 1 .391 4.536E3 .000 FORMULA .062 3 .021 239.086 .000 WAKTU .074

  a

  12 Tests of Between-Subjects Effects Dependent Variable:SLUDGE_DEGRADASI Source

  Value Label N FORMULA 1 K

  14

  3

  12

  7

  2

  12

  1

  9 WAKTU

  4 M3

  9

  3 M2

  9

  2 M1

  9

SLUDGE_DEGRADASI

  

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SLUDGE_DEGRADASI

  Duncan Subset

  WAKT U N

  1

  2

  3 12 .0467 7 12 .1083

  14 12 .1575 Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  Based on observed means. The error term is Mean Square(Error) = 8,61E-005.

SLUDGE_DEGRADASI

  Duncan Subset

  INTER AKSI N

  1

  2

  3

  4

  5

  6

  7 K0 3 .0033 M10 3 .0133 K7 3 .0367 M20 3 .0667 K14 3 .0833 M17 3 .0900 .0900 M30

  3 .1033

  M37

  3 .1467

  M27

  3 .1600

  M114

  3 .1767

  M214

  3 .1833

  M314

  3 .1867

  Sig. .199 1.000 1.000 .388 .091 .091 .224 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005.

3. Alat dan Bahan Penelitian

a. Alat-alat penelitian

  9

  3

  4

  5

  6

  7

  8

  11

  10

  17

  16

  18

  12

  13

  14

  15 ADLN Perpustakaan Universitas Airlangga

  2

  Keterangan:

  1. Pipet tip

  9. Botol aquades

  2. Sendok media

  3. Micropipet

  4. Pipet ukur

  5. Pipetor

  6. Botol kultur

  7. Bunsen

  8. Gelas ukur 10 mL

  10. Gelas ukur 500 mL

  18. Petri disk Timbangan analitik Spektrofotometer Hot plate

  11. Labu gojok

  12. Alumunium foil

  13. Kapas

  14. Masker face

  15. Pematik api

  16. Digi counter

  17. Gelas beker

  1

  

ADLN Perpustakaan Universitas Airlangga

Timbangan analitik Coloni Counter Autoclave

  Shaker Kulkas

  b. Bahan-bahan penelitian Foto Keterangan NB Spirtus Alkohol 70 % Oil Sludge N-Heksan Pewarna Gram

  N-heksan

  

ADLN Perpustakaan Universitas Airlangga

  4. Perlakuan dan Inkubasi Kontrol pada masa inkubasi 7 hari Konsorsium M1 pada masa inkubasi 7 hari

  Hasil Pour plate pada perlakuan kontrol Hasil Pour plate pada konsorsium M3 setelah 7 hari inkubasi setelah 14 hari inkubasi

  5. Data Formulasi

  a. Data Isolat Penyusun Konsorsium No. Nama konsorsium Jumlah isolat bakteri Kode isolat bakteri

  1. M1 7 jenis - S A2 A

• M B1 C - O A2 G - O A1 E - S A1 A - O A2 E1
• O A2 E2

  2. M2 5 jenis - SA2A

• MB1C
• OA2G
• OA1E
• SA1A

• MB1C
• OA2G

  1 SA2A Bulat Putih Flat Tidak rata

  6 OA2E1 Bulat Transparan Cembung Rata

  5 SA1A Bulat, Kecil

Putih,

Tengah

ada titik Flat Rata

  4 OA1E Bulat Putih, transparan Flat Rata

  3 OA2G Bulat, kecil Putih Flat Rata

  Flat Rata

  2 MB1C Bulat, besar

Putih,

bening

  Isolat Bentuk Koloni Warna Elevasi Tepi

  3. M3 3 jenis - SA2A

  

4 OA1E Vibrio spOA1E ( merujuk pada Vibrio alginoluticus dengan

tingkat kesamaan 94,97 %)

c. Karakteristik makroskopis isolat bakteri yang digunakan adalah sebagai berikut:

No. Kode

  3 OA2G Bacillus spOA2G (merujuk pada Bacillus stearothermophilus dengan tingkat kesamaan 65%)

  (merujuk pada Bacillus cereus dengan tingkat kesamaan 71%)

  2 S A2 A Bacillus spSA2A

  

1 M B1 C Pseudomonas Stutzeri (dengan tingkat kesamaan 93,70%)

  b. Hasil Uji Menggunakan Microbact Identification Kit

Setelah dilakukan identifikasi menggunakan Microbact Identification Kit 12A dan 12B, hasil

identifikasi pada isolat bakteri yang digunakan adalah sebagai berikut: No Kode Isolat Hasil Uji Menggunakan Microbact Identification Kit

  4. Kontrol Tanpa penambahan bakteri -

  7 OA2E2 Bulat Putih Flat Rata

ADLN Perpustakaan Universitas Airlangga d. Foto hasil isolat No. Kode Isolat Foto Koloni Foto Mikroskop dengan perbesaran 1000 X

  1 SA2A

  2 MB1C

  3 OA2G

  4 OA1E

  5 SA1A

  6 OA2E1

ADLN Perpustakaan Universitas Airlangga

  

ADLN Perpustakaan Universitas Airlangga