International Conference on Education, Technology, and Science NETS 2013 Improving The Quality of Education to Face The Impact of Technology

  

International Conference on Education, Technology, and Science

NETS 2013

Improving The Quality of Education to Face The Impact of Technology

PROCEEDINGS

  

Universitas Muhammadiyah Purwokerto

Purwokerto, December 28 th , 2013

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• • ) radicals. The treatment of ethanol extracts of purwoceng leaves with the scavenging activity of radical DPPH 38,65 ppm and NO
• • 28,88 ppm. The free radicals scavenging abilities of ethanol extracts of purwoceng leaves better than free radicals scavenging abilities of ethanol extracts of purwoceng herbs.

• • 14,73 ppm results IC30 smaller when compared with the ethanol extract of purwoceng herbs by the scavenging activity of radical DPPH 57,25 ppm and NO

  1. Material Test

  Materials

  The tools used in this research include: glass tools (measuring flask, measuring cup, test tubes, erlenmeyer flask), micro pipette, Ultrasonic bath, Rotary Evaporator, analytic scales, maceration, cupboard dryer, exicator, UV,Vis Spectrophotometer.

  RESEARCH METHODS Tool

  Saponin group is the one of chemical compound as secondary metabolites in purwoceng plant. There are also triterpenoid,steroids, sitosterol, and stigmasterol compounds. Purwoceng plant also contains coumarin, alkaloids, flavonoids, glycosides, and tannins. By looking at the content of secondary metabolites of purwoceng plant which are flavonoids and tannins (Haryono, 2013), is needful to do more researchs to find out the potential of the power of free radicals scavenging of purwoceng plant either the leaves or herbs because for specific flavonoid compounds are concentrated in the leaves (the herb Information, 2012; Waji et al, 2009).

  According to Caropeboka and Lubis (1975), root extract of purwoceng motor activity, which heightens tonus striated muscles, stimulates central nervous arrangement with capture point work on the medulla oblongata, sexual behavior and to improve male mice (Caropeboka et al., 1979 in S. Usmiati and S. Yuliana, 2010). So it is said that purwoceng’s root extract has an androgenic activity in mice.

  Purwoceng (Pimpinella pruatjan Molk.) is a medicinal plant. Commercially, purwoceng’s root has been widely reported have medicinal properties as aphrodisiac, diuretic, and tonic. This plant is native plant from Indonesia which live in endemic areas, such as mountainous Dieng plateau in Central Java, mount Pangrango, West Java and East Java’s mountainous areas.

  Flavonoids are natural phenolic compounds are potentially as free radicals scavenger and have bioactivities as a medication. Generally, leaf, root, stem and flower are the part of plant that save many flavonoids as secondary metabolites (Waji et al, 2009). Another natural phenolic compounds that potential as a free radicals scavenger is tannin. Tannins can be found in all parts of the plant, but at some plants, tannin concentrated on specific parts (Hernawan and Setyawan, 2003). One of the plants that contain flavonoids and tannins as secondary metabolites is purwoceng (Haryono, 2013).

  Free radicals is an atom or group of atoms that has a highly reactive characteristic caused by the presence of one or more free electrons or electrons are not paired, so that it becomes unstable and high, energy (Fessenden and Fessenden, 1982). To avoid the free radicals attack, is by consuming natural antioxidants in sufficient amount each day. Anti, free radical is a compound which in a small amount when compared with the substratum is capable of delaying or preventing or avoiding the oxidation of substrates which are easily oxidized (Halliwell and Gutteridge, 2000).

  INTRODUCTION

  Keywords: free radicals, Purwoceng, DPPH, nitric oxide

  Abstract Flavonoids and tannins are potentially as free radicals scavenger. Purwoceng has some of the compounds such as flavonoid, glycosides, and tannins. Research done by using DPPH and Nitric oxide (NO

  

LEAVES

Balqis Hisyam Saleh Basleman, Wiranti Sri Rahayu, Retno Wahyuningrum

Pharmacy Faculty of Muhammadiyah University of Purwokerto

  , 2013

THE COMPARISON OF FREE RADICALS SCAVENGING ABILITIES BETWEEN

ETHANOL EXTRACTS OF PURWOCENG (#impinella pruatjan Molk.) HERBS AND

  Proceedings of International Conference On Education, Technology and Science (NETS) 2013 Purwokerto, December 28 th

  Ethanol extracts of purwoceng herbs and ethanol extracts leaves (Pimpinella pruatjan Molk.). Proceedings of International Conference On Education, Technology and Science 2013 Purwokerto, December 28 th

  , 2013

2. Chemicals

  a. Preparation of Test Materials

  The magnitude of resource free radicals scavenging is calculated using the formula: Resource free radicals scavenging

  By dissolving 1,0 mL of reagent 0.33% sulfanilat acid in glacial acetic acid (20%), then incubated for 5 minutes at room temperature. Then add 1.0 mL of N,(1, Naphthyl), ethylenediamine dihydrochloride 0.1% (w/v).

  Dissolve 299,12 mg of the powder of sodium nitroprusside in methanol p.a. on pumpkin measures 100,0/250 mL, then divorteks. 2) Griess Reagent Creation

  1) Making a solution of Sodium Nitroprusside 10 mM

  c. Determining Free Radicals Scavenging Quantitatively with Nitric Oxide Radicals (NO

• ^• )

  x 100% The acquired absorbance data was created a linear regression equation which expressing the relationship between the concentration of the test substance (x) with the average antioxidant activity (y) of a series of measurements of replication so retrieved IC50 value, is the test solution that necessary scavenge 50% of DPPH radical over the past 30 minutes (operating time), or pause time required by the test solution to reduce the radical DPPH perfectly. After 30 minutes the absorbance obtained will be constant.

  Abs. Control

  Control $ Abs. Sample

  % = Abs.

  30 minutes, the absorbance is measure at a maximum wavelength. Measure the negative control absorbance also without the addition of the test solution.

  1) Preparation of simplicia Cultivation purwoceng plants is obtained from Dieng’s farmer, Wonosobo,

  Ethyl acetate technical degrees, petroleum ether technical degrees, ethanol 96% technical degrees, ethanol p.a., DPPH, sodium nitroprusside, sulfanilic acid p.a., glacial acetic acid p.a., N,(1, Naphthyl),ethylenediamine dihydrochloride, Griess reagent p.a, BHT (butilatedhydroxytoluene), quercetin.

  Ethanol extracts of purwoceng leaves and ethanol extracts of purwoceng herbs dissolved in methanol p.a. with a concentration of 0.01% w/v. 4) Test Power Free Radicals Scavenging

  3) Preparation of Ethanol Extract of Purwoceng

  2) Determination of Maximum Wavelength of DPPH Solution Determination of the maximum wavelength of DPPH 0,4 mM are used in the power of free radicals scavenging test is done as follows: 1000 eL DPPH solution 0,4 mm added to the volume with methanol 5 mL then observed the absorbance in the range of wavelength 400,800 nm using methanol p.a. as blank.

  1) Preparation of DPPH solution 0,4 mM DPPH solution of 0.4 mM is made by dissolving 15.8 mg powder DPPH in methanol p.a. on pumpkin measures 100.0 mL, then vortex.

  b. Determining Free Radicals Scavenging Quantitatively with DPPH Radicals

  The dry simplicia powder was added to the container maceration. Done,with use 3 concentrate solution of maceration, namely petroleum ether (PE), ethyl acetate (EA) and ethanol 96%. Each maceration with a different concentrate performed as many as 3x24 hours in order to obtain optimal results maceration. During submersion, stirring for 1 hour was done so that the compounds contained in the simpilisia of purwoceng leaves and herbs can dissolve properly.

  Central Java. Purwoceng plants that have been obtained are cleaned from impurities attached to later washed until clean. The plant had been dried with dryer cupboard. Further divided two groups that is group with the herbs and group with the leaves only. Then powdered and sifted with fine powder 20 degrees so that the obtained powders with a uniform size (anonymous, 1979). 2) Extraction of simplicia

  As many as 1000 eL DPPH 0.4 mM put in pumpkin measures, added by the test solution with different volume series (500, 1000, 1500, 2000 and 2500 eL) is then added methanol p.a. up to 5.0 mL volume, vortex for 1 minute until the mixture is homogenous. After Proceedings of International Conference On Education, Technology and Science (NETS) 2013 th

  Purwokerto, December 28 , 2013

  3) Determination of Maximum Wavelength 2) Quercetin Stock solution 100 ppm Sodium Nitroprusid 10 mM The stock solution is made by

  Determination of the maximum weighing carefully 0,01 grams of quercetin wavelength of Sodium Nitroprusside 10 mM and dissolved in 100 mL of methanol p.a. used in the power of free radicals 3) Test Power Free Radicals Scavenging scavenging test is done as follows: 2000 eL The power free radicals scavenging test sodium nitroprusside solution 10 mM added for a positive control is carried out with the with 500 mL of 7.4 pH phosphate buffer and power free radical scavenging test of ethanol proceed with incubation at a temperature of extract of herbs ethanol extract leaves 25°C for 150 minutes, then added with 500 purwoceng, but the series volume control eL Griess reagent and measured the that is added is different. For BHT the absorbance in the range of wavelength 400, volume series are 25, 50, 100, 200 and 400 800 nm using methanol p.a. as blank. eL of a stock solution of 100 ppm and to 4) Determination of Operating Time of measure the absorbance was used methanol

  Coupling Compound as blank, for quercetin the volume series are Determination of operating time of 2, 4, 8, 16 and 32 eL of a stock solution of coupling compound was done by measuring 100 ppm and to measure the absorbance was the mixture of 2000 eL sodium nitroprusside used same concentration of quercetin on test 10 mM and 500 eL phosphate buffer (pH resource free radicals scavenging as blank.

  7.4) and the Griess reagent. Then measure the operating time mixture solution at a RESULT AND DISCUSSION maximum wavelength by using methanol

  1. Determination of Purwoceng Plant

  p.a. as blank. The results of determination for purwoceng 5) Preparation of Ethanol Extract of plant which is done according to the book the Flora of Java (Backer and Bakhuizen, 1965) is retrieved

  Purwoceng Ethanol extracts of purwoceng leaves the key determination stating the plant used is and ethanol extracts of purwoceng herbs Pimpinella pruatjan Molkenb. with synonym dissolved in methanol p.a. with a Pimpinella alpina Molk. concentration of 0.01% w/v.

  2. Extraction of Purwoceng Plant

  6) Test Power Free Radicals Scavenging 2.0 mL solution of sodium