Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed Macaque Populations in Bali Island, Indonesia.
Editorial Team
Editor-in-Chief
1.
D r . d r h . I Nen g ah Wan d ia ,
[ SCOPUS I D: 24492621500, h- index: 2] Progam Magist er Kedokt eran
Hewan, Program Pascasarj ana, Universit as Udayana, Denpasar, Bali, I ndonesia
Associate Editor
1.
Pr o f . D r . d r h . I K e t u t Pu j a ,
[ SCOPUS I D: 55342151000, h- index: 1] Progam Magist er Kedokt eran
Hewan, Program Pascasarj ana, Universit as Udayana, Denpasar, Bali, I ndonesia
2.
Pr o f . D r . d r h . I K e t u t B e r a t a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
3.
Pr o f . D r . d r h . I w a n H a r j a Ut a m a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana,
Universit as Udayana, Denpasar, Bali, I ndonesia
4.
D r . d r h . H a p s a r i Ma h a t m i ,
Pr ogam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
5.
D r . d r h . I GN S u d i s m a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
6.
Dr . d r h . I Ny o m an Su ar t h a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
Editorial Board
1.
d r h . I Ge d e S o m a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as Udayana,
Denpasar, Bali, I ndonesia
2.
D r . d r h . N L Ek a S e t i a s i h ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
3.
d r h . I PG Yu d h i A r j e n t i n i a ,
Pr ogam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
4.
I d a B a g u s Yo g i I s w a r a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
Reviewer
1.
D r . d r h . Co k Pu t r a Pe m a y u n ,
Lab. Reproduksi Vet eriner FKH Unud, Denpasar, I ndonesia
2.
Pr o f . D r . En d a n g Tr i Ma r g a w a t i ,
3.
D r . d r h . I W a y a n Ma s a Te n a y a ,
Lab. Genet ika Molekuler Hewan LI PI , Cibinong, Bogor, I ndonesia
[ SCOPUS I D: 6505560482, h- index: 3) Bagian Penyakit Hewan Menular
Balai Besar, Vet eriner, Denpasar, I ndonesia
4.
D r . d r . Ma d e Ja w i ,
Bagian Farm akologi FK Unud, Denpasar, I ndonesia
5.
D r . d r . I D N. Su k r a m a ,
6.
Pr o f . D r . d r h . I B K A r d a n a ,
7.
Pr o f . D r . I K e t u t Ju n i t a ,
8.
Pr o f . D r . d r h . GA . Yu n i a t i K e n c a n a ,
9.
Pr o f . D r . d r h . I N y o m a n S u a r s a n a ,
Bagian Biologi Molekuler FK Unud, Denpasar, I ndonesia
Lab. Pat ologi Klinik FKH Unud, Denpasar, I ndonesia
Lab. Genet ika FMI PA Unud, Denpasar, I ndonesia
Lab. Virologi Vet eriner FKH Unud, Denpasar, I ndonesia
Lab. Biokim ia FKH Unud, Denpasar, I ndonesia
10. Pr o f . D r . d r h . A A A M i r a h A d i , Lab. Pat ologi FKH Unud, Denpasar,, I ndonesia
11. D r . Em m S v e n s s o n , Division of Molecular Genet ics, Swedish Universit y of Agricult ural Science.
UPPSALA, Swedia
Vol 2, No 2 (2014)
Table of Contents
Articles
Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed PDF
Macaque Populations in Bali Island, Indonesia
I Nengah Wandia, I Gede Soma, I Gusti Agung Arta Putra
Seroprevalensi Neospora caninum pada sapi bali yang dipotong di PDF
Rumah Potong Hewan (RPH) Denpasar
Adryani Ris, Nyoman Sadra Dharmawan, I Made Damriyasa
Asosiasi Lokus Mikrosatelit BMS1282 dengan Kejadian Kawin Berulang PDF
pada Sapi Bali
Yesy Febnica Dewi, I Nengah Wandia, I Ketut Puja
Potensi Serbuk Daun Kelor (Moringa oleifera) Sebagai Anthelmintik PDF
Terhadap Infeksi Ascaris suum dan Feed Supplement pada Babi
Muhammad Ulqiya Syukron, I Made Damriyasa, Nyoman Adi
Suratma
Asosiasi Polimorfisme Mikrosatelit DRBP1 Gen BoLa (Bovine Leucocyte PDF
Antigen) dengan Ukuran Tubuh pada Sapi Bali
Ni Wayan Patmawati, I Nengah Wandia, I Ketut Puja
Pengaruh Berbagai Konsentrasi Dimethylsulfoxide terhadap Kualitas PDF
Semen Beku Ayam Hutan Hijau Post Thawing
Wayan Bebas, DND Indira Laksmi
Polimosfisme Lokus Mikrosatelit BM1329 dan Hubungannya dengan PDF
Calving Interval pada Sapi Bali
Made Raysa Merliana, I Nengah Wandia, I Ketut Puja
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
Vol 2 No 2: 61-70
Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed
Macaque Populations in Bali Island, Indonesia
Polimorfisme Lokus Mikrosatelit Kromosom Y pada Populasi Monyet Ekor
Panjang di Pulau Bali, Indonesia
I Nengah Wandia 1,2*, I Gede Soma1,3, I Gusti Agung Arta Putra1,4
1 Primate Research Center, Udayana University
2 Laboratory. of Veterinary Anatomy, Faculty of Vet. Medicine, Udayana University
3 Laboratory. of veterinary physiology, Faculty of Vet. Madice, Udayana University
4 Laboratory. of Nutrition & Feed Animal, Faculty of Animal Husbandry, Udayana
University
*corresponding author, email: wandia@fkh.unud.ac.id
ABSTRAK
Monyet ekor panjang tersebar di seluruh wilayah di Pulau Bali, tetapi, kini
keberadaannya telah terpisah-pisah menjadi populasi lokal yang lebih kecil. Walupun
demikian, laporan yang melukiskan modus fragmentasi dan struktur genetik populasi
belum banyak ditemukan. Penelitian ini bertujuan untuk mengeksplorasi polimorfisme
lokus mikrosatellite kromosom Y pada populasi monyet ekor panjang di Pulau Bali
dengan menggunanakan tiga primer mikrosatelit manusia, yaitu DYS390, DYS391, dan
DYS393. Sejumlah 99 sampel darah dikoleksi dari monyet ekor panjang jantan yang
berasal dari 8 populasi (23 dari Pulaki, 11 dari Bedugul, 13 dari Mekori, 8 dari Sangeh, 6
dari Uluwatu, 11 dari Alas Kedaton, 11 dari Ubud, dan 16 dari Bukit Gumang). DNA
total diekstraksi dengan QIAamp® Blood Mini Kit. Polimorfisme lokus mikrosatelit
dideteksi menggunkan teknik PCR, dan variasi alel dipisahkan melalui elektroforesis
pada 7% gel poliakrilamid. Hasil penelitian menunjukkan bahwa lokus DYS390 dan
DYS393 adalah monomorfik (masing-masing memiliki satu alel). Lokus DYS391
mempunyai dua alel, karena itu, lokus ini adalah polimorfik. Dari hasil penelitian dapat
disarankan bahwa lokus DYS391 dapat digunakan untuk studi variasi genetik populasi
monyet ekor panjang jantan di Pulau Bali.
Kata kunci: monyet ekor panjang, polimorfisme, lokus mikrosatelit, kromosom Y, Pulau
Bali
ABSTRACT
The long tailed macaques inhabit throughout Bali Island, however, recently they have
been fragmented into many smaller local populations. In spite of that, there are just few
reports that describe the mode of fragmentation and the genetic structure of the
population. This research aimed to explore the polymorphism of microsatellite loci on Y
chromosome in long tailed macaque populations in Bali Island using three human
microsatellite primers, namely DYS390, DYS391, and DYS393. A total of 99 blood
samples were collected from male long tailed macaques originated from 8 populations
(23 from Pulaki, 11 from Bedugul, 13 from Mekori, 8 from Sangeh, 6 from Uluwatu, 11
from Alas Kedaton, 11 from Ubud, and 16 from Bukit Gumang). Total DNA was
extracted using QIAamp® Blood Mini Kit. The polymorphism of the microsatellite loci
was detected using PCR technique, and allelic variations were separated through 7% poly
acrylamide gel electrophoresis. The result of the research showed that DYS390 and
61
Wandia et al.
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
DYS393 loci were monomorphic (each had only one allele). The DYS391 locus had two
alleles, therefore, this locus was polymorphic. It could be suggested that DYS391 locus
could be used to study genetic variation in male long tailed macaque population in Bali
Island.
Key words: long tailed macaques, polymorphism, microsatellite locus, Y chromosome,
Bali Island
flow homogenizes the genetic variation
INTRODUCTION
inter population (Nozawa et al., 1996;
(Macaca
Hartl & Clark, 1997; Li, 1997). However,
fascicularis) is a nonhuman primate that
the male migration some time gives a
has distributed worldwide (Euday, 1980;
negative effect, that is, it intruduces a
Fooden, 1980; Napier and Napier, 1985).
pathogen agent to the new population or
Nearly all islands of Indonesia have been
group in which the pathogen agent can
dwelling by the macaques, and because of
cause an illness or contagious disease
their high adaptation potency, the animals
(Wandia, 2003).
Long
tailed
macaque
can live in various habitats (Napier-
Many tourist destinations in Bali
Napier, 1985; Fooden, 1995; Rowe, 1996).
Island are also a habitat of the long tailed
In the island of Bali, the macaques have
macaques
dispersed almost the regions and taken
Kedaton, Uluwatu, Pulaki, and Bedugul.
place in primary and secondary forests,
The macaques in such location give a
riverine, and coastal area (Southern, 2002).
positive impact, so that, the locations have
The long tailed macaque is a female
been known as the monkey tourist
philopatry i.e. live in social group in which
destination. Many non genetic researches
females remain and breed in the group of
have been carried out in these long tailed
their birth. The females compose a nuclear
macaques (Loudon et al., 2006; Lissa et
members of the social group (Joly, 1985;
al., 2008; Lane et al., 2010;, Schilaci et
Napier-Napier,
Perwitasari-
al., 2010; Brotcorne et al., 2011; Fuentes
Farajallah et al., 2004). Different from the
et al., 2011; Suarjana & Asmara, 2012).
female, male macaques will not stay firmly
The research about genetic variation in
in natal group, but migrate to other group
long tailed macaque population in Bali has
during their growing up period. The male
been conducted by Kawamoto et al.
migration would be a genetic bridge for
(1994) using blood protein marker. Other
entering the source of genetic variation to
research
the new population or group. This gene
microsatellite
1985;
62
like
i.e.
Sangeh,
Ubud,
characterization
locus
on
Alas
of
somatic
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
Vol 2 No 2: 61-70
13 samples.
chromosome has been carried out by
Wandia (2003, 2004a, 2004b), and Rell et
The total DNA was extracted using
al. (2013). However, there was no study
QIAamp® DNA Blood Kits according to
that exploring the genetic variation using
company protocol (Qiagen, 2007). Three
molecular marker on Y chromosome of the
microsatellite loci on Y chromosome were
long tailed macaques. This research aimed
examined
to characterize the microsatellite loci on Y
human microsatellite primer (DYS390,
chromosome of long tailed macaque
DYS391 and DYS393). The loci were
populations
amplified by polymerase chain reaction
in
the
Island
of
Bali,
their
tecnique,
polymorphism
and
each
using
Indonesia. The polymorphic information
(PCR)
reaction
of loci is very important before using them
contained 4 mM MgCl2; dNTPs each 0.16
as molecular marker for assessing the
mM; a pair of primers each 0.4 mM; 0.7 U
DNA Polymerase; 1.25 l PCR buffer
genetic variation within and between
(10x), and 1 l template DNA. Pure water
populations.
was added to make the final volume of
reaction 12.5 l (Hillis et al., 1996). PCR
MATERIALS AND METHOD
for
DYS390
locus
was:
Pre
PCR:
A total of ninety nine male long tailed
denaturation (94o C) for 3 minutes; PCR:
macaque blood samples was subjected for
denaturation (94o C) 35 seconds, annealing
DNA extraction. The blood samples were
(50o C) 35 seconds, and elongation (72o C)
collected in the year 2010-2011 by
35 seconds; post PCR: elongation (72o C)
research team of Primate Research Center
5 minutes. PCR was replicated 30 cycles
Udayana University. The macaques were
using Applied Biosystems 2720 Thermal
anaesthetized with the combination of
Cycler. The PCR for DYS391 and
Ketamin HCl (dose 10 mg/kg body
DYS393 was the same as those of
weight) and Xylazin with ratio 5:1, and the
DYS390 with the exception of annealing
drug was injected using blow pipe. These
temperature at 54o C. The PCR product
samples
local
was separated by 7% PAGE in 1x TBE
populations in Bali Island, namely Sangeh
buffer and run at 125 Volt for 90 minutes.
8 samples, Uluwatu 6 samples, Alas
The allelic variation was developed using
Kedaton 11 samples, Ubud 11 samples,
silver staining, and the length of allele was
Bukit Gumang 16 samples, Pulaki 23
measured by aligning the allelic band with
samples, Bedugul 11 samples, and Mekori
the bands of 100 base pairs ladder of
originated
from
eight
63
Wandia et al.
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
molecular marker. The polymorphism of
length of the alleles was 282 base pairs
loci was analyzed using Alrequin Ver 3.5
and 292 base pairs. This locus was
soft ware (Excoffier & Lischer, 2010).
polymorphic, however, the two alleles
were distributed oddly.
The allele 292
(DYS391292) was found in all populations,
RESULTS AND DISCCUSIONS
in contrary, the allele 282 (DYS391282)
Results
was just found in population of Pulaki.
Distribution of alleles and haplotypes
Two other loci (DYS390 and DYS393)
were monomorphic as long as these loci
The results showed that two alleles
just had one allele (Table 1).
were found in DYS391 locus which the
Table 1. Allele Distribution of the Microsatellite Loci on Y Chromosome of Long Tailed
Macaque Populations in Bali Island
Relative Frequencies of allele in sampling site
No
Loci
Allele
Total
PL
n=23
BD
MK
n=11 n=13
SG
n=8
AK
n=11
UW
n=6
UB
BG
Bali
n=11 n=16 n=99
1
DYS390 DYS390240
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
2
DYS391 DYS391292
0.91
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.98
DYS391282
0.09
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.02
DYS393 DYS393272
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
3
Mapping of the three loci in one individual of Y chromosome will compose the
haplotype. Genetic analysis showed that there were two kinds of haplotype, in which, one
of them was only distributed to population of Pulaki (Table 2).
Table 2. Haplotype distribution of Y Chromosome of Long Tailed Macaque Populations
in Bali Island
Relative frequencies of haplotype in population
Haplotype
hp composition
1
2
Total
PL
BD
MK
n=23 n=11 n=13
SG
n=8
AK
n=11
UW
n=6
UB
BG
Bali
n=11 n=16 n=99
hp240:292;272
0.91
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.98
hp240;282; 272
0.09
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.02
64
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
Vol 2 No 2: 61-70
Locus and Haplotype Diversities of Y Chromosome of Long Tailed Macaque
Populations in Bali Island
Locus and haplotype diversities of Y chromosome of long tailed macaque
populations were very low, or even zero in most populations (Table 3). It has some thing
to do with the monomorphic condition of the loci. Polymorphic condition of DYS391
locus in population of Pulaki has contributed to the value of haplotype diversity
(hd=0.17).
Tabel 3 Locus and Haplotype Diversities of Y Chromosome of Long Tailed Macaque
Populations in Bali Island
Diversity in populations
Total
Source
PL
BD
MK
n=23 n=11 n=13
SG
n=8
AK
n=11
UW
n=6
UB
BG
Bali
n=11 n=16 n=99
DYS390 locus
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
DYS391 locus
0.17
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.04
DYS393 locus
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Haplotype (hd)
0.17
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.04
replication and due to the effective number
Disccusions
A Locus polymorphism level is
of the variation source is a fourth apart of
supplied by the number of genetic
those of the somatic chromosome, this
variation sources in a population. The
genetic marker will reach a fixation in
variation of mtDNA genetic marker comes
shorter evolutionary time in a population
from one source, mtDNA of the female
compare to the genetic marker of somatic
(Walace et al., 1999; Ingman et al., 2000;
chromosome. The resulth of this research
Bowels et al., 2007), likewise, the
exibited three allales of the four alleles
variation of a genetic marker of Y
found were fixed or nearly fixed in all
chromosome
populations. Nearly fixation state of those
just comes from
Y
chromosome beared by the male (Evans et
alleles may associate with
al., 2010; Rovie-Rian et al., 2013). As
recombination during replication and low
long as a genetic marker of Y chromosome
effective number of variation source as
does not undergo a recombination during
describes above. There was only one allel,
65
no DNA
Wandia et al.
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
DYS391282, had low frequency and just
populations in Bali Island was lower than
found in population of Pulaki (Tabel 1).
those
This may reflect a new mutation of allele,
populations in Lombok Island. The botlle
therefore, the male bearing the allele has
neck effect may decrease severely the total
not had enough chances to distribute the
number of the population members, and so
allele through mating with a female within
do the number of the male. While this
a population as well as migrating to other
preliminary
populations.
microsatellite loci on Y chromosome and
of
the
long
tailed
resarch
macaque
used
three
The low haplotype diversities are not
only one of them was polimorphic, the
only affected by the total number of
data may not express the real condition. It
geneticaly different male in population,
would
but also the number of loci used and the
individual
polimorphism of the loci (Perwitasari-
populations.
Farajallah et al., 2004). Low haplotype
pertaining to the evolutionary history of
diversities of Y chromosome may reflects
male macaques in Bali Island, a further
to a few genetically different male of long
research
taild macaques in wild in Bali Island. The
microsatellite loci on Y chromosome is
long tailed macaques in Bali Island were
needed. Moreover, a broaden sampling
originated from Java Island and they
sites
migrated during the two islands unite to be
including population in Java Isalnd and
a part of Sunda Selft (Eudey 1980; Fooden
Lombok
1995). However, there is no a clear
evolutionary history of male macaque in
information about the
of
South Archipelago of Indonesia. However,
migration events and the total number of
this research has succeeded to characterize
different populations had migrated. This
three microsatellite loci on Y chromosome
lack of information brings to the difficulty
at native populations of long tailed
to justify the male number of long tailed
macaque in Bali Island.
frequencies
be
weak
both
(population)
Island
differentiating
within
To
using
in
and
better
a
understand
more
may
to
between
be
polimorfic
obligated
elucidate
the
macaques as the founder population in
Bali Island in the past. Kawamoto et al.
CONCLUSION
(1984) stated that the long tailed macaques
populations in Bali Island had suffered
Based on the result of the research, it
from a bottle neck effect. It was based on
can be concluded that each of DYS390 and
the
DYS393 loci has one allele, so, these two
blood protein data that the genetic
variation
of
long
tailed
macaque
loci are monomorphic in male long tailed
66
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
Vol 2 No 2: 61-70
macaques in Bali Island. On the other
Indonesia. In Monkeys on the Edge.
hand, DYS391 locus that having two
Ecology and Management of Long
alleles is polymorphic. The locus of
Tailed Macaques and Their Interface
DYS391 could be used in the study of
with Humans. Editors M.D. Gumert,
genetic differentiation between male long
A.
tailed macaque populations in Bali Island,
Cambridge University Press, Pp 180-
but not the two others.
182.
Fuentes,
and
L.Jones-Engel.
Eudey AA. 1980. Pleistocene glacial
phenomena and the evolution of Asian
ACKNOWLEDGEMENT
macaques. In The Macaques. Studies
My thanks
to
the President
of
in Ecology, Behavior and Evolution .
Udayana University for grand approval on
Edited by D.G. Lindburg. :52-83.
this research with the Contract No:
Evans BJ, Pin L, Melnick DJ, Wright SI.
21.4/UN14/KU.03.04/2012, on the date of
2010.
May, 16 2012 and the Contract No:
macaque monkeys: Implications for
174.17/UN14.2/PNL.01.03.00/2013,
effective population size and dispersal
on
the date of May,16 2013.
Sex-linked
inheritance
in
to Sulawesi. Genetics 185: 923–937 (
July 2010). Copyright _ 2010 by the
Genetics Society of America DOI:
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Editor-in-Chief
1.
D r . d r h . I Nen g ah Wan d ia ,
[ SCOPUS I D: 24492621500, h- index: 2] Progam Magist er Kedokt eran
Hewan, Program Pascasarj ana, Universit as Udayana, Denpasar, Bali, I ndonesia
Associate Editor
1.
Pr o f . D r . d r h . I K e t u t Pu j a ,
[ SCOPUS I D: 55342151000, h- index: 1] Progam Magist er Kedokt eran
Hewan, Program Pascasarj ana, Universit as Udayana, Denpasar, Bali, I ndonesia
2.
Pr o f . D r . d r h . I K e t u t B e r a t a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
3.
Pr o f . D r . d r h . I w a n H a r j a Ut a m a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana,
Universit as Udayana, Denpasar, Bali, I ndonesia
4.
D r . d r h . H a p s a r i Ma h a t m i ,
Pr ogam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
5.
D r . d r h . I GN S u d i s m a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
6.
Dr . d r h . I Ny o m an Su ar t h a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
Editorial Board
1.
d r h . I Ge d e S o m a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as Udayana,
Denpasar, Bali, I ndonesia
2.
D r . d r h . N L Ek a S e t i a s i h ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
3.
d r h . I PG Yu d h i A r j e n t i n i a ,
Pr ogam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
4.
I d a B a g u s Yo g i I s w a r a ,
Progam Magist er Kedokt eran Hewan, Program Pascasarj ana, Universit as
Udayana, Denpasar, Bali, I ndonesia
Reviewer
1.
D r . d r h . Co k Pu t r a Pe m a y u n ,
Lab. Reproduksi Vet eriner FKH Unud, Denpasar, I ndonesia
2.
Pr o f . D r . En d a n g Tr i Ma r g a w a t i ,
3.
D r . d r h . I W a y a n Ma s a Te n a y a ,
Lab. Genet ika Molekuler Hewan LI PI , Cibinong, Bogor, I ndonesia
[ SCOPUS I D: 6505560482, h- index: 3) Bagian Penyakit Hewan Menular
Balai Besar, Vet eriner, Denpasar, I ndonesia
4.
D r . d r . Ma d e Ja w i ,
Bagian Farm akologi FK Unud, Denpasar, I ndonesia
5.
D r . d r . I D N. Su k r a m a ,
6.
Pr o f . D r . d r h . I B K A r d a n a ,
7.
Pr o f . D r . I K e t u t Ju n i t a ,
8.
Pr o f . D r . d r h . GA . Yu n i a t i K e n c a n a ,
9.
Pr o f . D r . d r h . I N y o m a n S u a r s a n a ,
Bagian Biologi Molekuler FK Unud, Denpasar, I ndonesia
Lab. Pat ologi Klinik FKH Unud, Denpasar, I ndonesia
Lab. Genet ika FMI PA Unud, Denpasar, I ndonesia
Lab. Virologi Vet eriner FKH Unud, Denpasar, I ndonesia
Lab. Biokim ia FKH Unud, Denpasar, I ndonesia
10. Pr o f . D r . d r h . A A A M i r a h A d i , Lab. Pat ologi FKH Unud, Denpasar,, I ndonesia
11. D r . Em m S v e n s s o n , Division of Molecular Genet ics, Swedish Universit y of Agricult ural Science.
UPPSALA, Swedia
Vol 2, No 2 (2014)
Table of Contents
Articles
Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed PDF
Macaque Populations in Bali Island, Indonesia
I Nengah Wandia, I Gede Soma, I Gusti Agung Arta Putra
Seroprevalensi Neospora caninum pada sapi bali yang dipotong di PDF
Rumah Potong Hewan (RPH) Denpasar
Adryani Ris, Nyoman Sadra Dharmawan, I Made Damriyasa
Asosiasi Lokus Mikrosatelit BMS1282 dengan Kejadian Kawin Berulang PDF
pada Sapi Bali
Yesy Febnica Dewi, I Nengah Wandia, I Ketut Puja
Potensi Serbuk Daun Kelor (Moringa oleifera) Sebagai Anthelmintik PDF
Terhadap Infeksi Ascaris suum dan Feed Supplement pada Babi
Muhammad Ulqiya Syukron, I Made Damriyasa, Nyoman Adi
Suratma
Asosiasi Polimorfisme Mikrosatelit DRBP1 Gen BoLa (Bovine Leucocyte PDF
Antigen) dengan Ukuran Tubuh pada Sapi Bali
Ni Wayan Patmawati, I Nengah Wandia, I Ketut Puja
Pengaruh Berbagai Konsentrasi Dimethylsulfoxide terhadap Kualitas PDF
Semen Beku Ayam Hutan Hijau Post Thawing
Wayan Bebas, DND Indira Laksmi
Polimosfisme Lokus Mikrosatelit BM1329 dan Hubungannya dengan PDF
Calving Interval pada Sapi Bali
Made Raysa Merliana, I Nengah Wandia, I Ketut Puja
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
Vol 2 No 2: 61-70
Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed
Macaque Populations in Bali Island, Indonesia
Polimorfisme Lokus Mikrosatelit Kromosom Y pada Populasi Monyet Ekor
Panjang di Pulau Bali, Indonesia
I Nengah Wandia 1,2*, I Gede Soma1,3, I Gusti Agung Arta Putra1,4
1 Primate Research Center, Udayana University
2 Laboratory. of Veterinary Anatomy, Faculty of Vet. Medicine, Udayana University
3 Laboratory. of veterinary physiology, Faculty of Vet. Madice, Udayana University
4 Laboratory. of Nutrition & Feed Animal, Faculty of Animal Husbandry, Udayana
University
*corresponding author, email: wandia@fkh.unud.ac.id
ABSTRAK
Monyet ekor panjang tersebar di seluruh wilayah di Pulau Bali, tetapi, kini
keberadaannya telah terpisah-pisah menjadi populasi lokal yang lebih kecil. Walupun
demikian, laporan yang melukiskan modus fragmentasi dan struktur genetik populasi
belum banyak ditemukan. Penelitian ini bertujuan untuk mengeksplorasi polimorfisme
lokus mikrosatellite kromosom Y pada populasi monyet ekor panjang di Pulau Bali
dengan menggunanakan tiga primer mikrosatelit manusia, yaitu DYS390, DYS391, dan
DYS393. Sejumlah 99 sampel darah dikoleksi dari monyet ekor panjang jantan yang
berasal dari 8 populasi (23 dari Pulaki, 11 dari Bedugul, 13 dari Mekori, 8 dari Sangeh, 6
dari Uluwatu, 11 dari Alas Kedaton, 11 dari Ubud, dan 16 dari Bukit Gumang). DNA
total diekstraksi dengan QIAamp® Blood Mini Kit. Polimorfisme lokus mikrosatelit
dideteksi menggunkan teknik PCR, dan variasi alel dipisahkan melalui elektroforesis
pada 7% gel poliakrilamid. Hasil penelitian menunjukkan bahwa lokus DYS390 dan
DYS393 adalah monomorfik (masing-masing memiliki satu alel). Lokus DYS391
mempunyai dua alel, karena itu, lokus ini adalah polimorfik. Dari hasil penelitian dapat
disarankan bahwa lokus DYS391 dapat digunakan untuk studi variasi genetik populasi
monyet ekor panjang jantan di Pulau Bali.
Kata kunci: monyet ekor panjang, polimorfisme, lokus mikrosatelit, kromosom Y, Pulau
Bali
ABSTRACT
The long tailed macaques inhabit throughout Bali Island, however, recently they have
been fragmented into many smaller local populations. In spite of that, there are just few
reports that describe the mode of fragmentation and the genetic structure of the
population. This research aimed to explore the polymorphism of microsatellite loci on Y
chromosome in long tailed macaque populations in Bali Island using three human
microsatellite primers, namely DYS390, DYS391, and DYS393. A total of 99 blood
samples were collected from male long tailed macaques originated from 8 populations
(23 from Pulaki, 11 from Bedugul, 13 from Mekori, 8 from Sangeh, 6 from Uluwatu, 11
from Alas Kedaton, 11 from Ubud, and 16 from Bukit Gumang). Total DNA was
extracted using QIAamp® Blood Mini Kit. The polymorphism of the microsatellite loci
was detected using PCR technique, and allelic variations were separated through 7% poly
acrylamide gel electrophoresis. The result of the research showed that DYS390 and
61
Wandia et al.
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
DYS393 loci were monomorphic (each had only one allele). The DYS391 locus had two
alleles, therefore, this locus was polymorphic. It could be suggested that DYS391 locus
could be used to study genetic variation in male long tailed macaque population in Bali
Island.
Key words: long tailed macaques, polymorphism, microsatellite locus, Y chromosome,
Bali Island
flow homogenizes the genetic variation
INTRODUCTION
inter population (Nozawa et al., 1996;
(Macaca
Hartl & Clark, 1997; Li, 1997). However,
fascicularis) is a nonhuman primate that
the male migration some time gives a
has distributed worldwide (Euday, 1980;
negative effect, that is, it intruduces a
Fooden, 1980; Napier and Napier, 1985).
pathogen agent to the new population or
Nearly all islands of Indonesia have been
group in which the pathogen agent can
dwelling by the macaques, and because of
cause an illness or contagious disease
their high adaptation potency, the animals
(Wandia, 2003).
Long
tailed
macaque
can live in various habitats (Napier-
Many tourist destinations in Bali
Napier, 1985; Fooden, 1995; Rowe, 1996).
Island are also a habitat of the long tailed
In the island of Bali, the macaques have
macaques
dispersed almost the regions and taken
Kedaton, Uluwatu, Pulaki, and Bedugul.
place in primary and secondary forests,
The macaques in such location give a
riverine, and coastal area (Southern, 2002).
positive impact, so that, the locations have
The long tailed macaque is a female
been known as the monkey tourist
philopatry i.e. live in social group in which
destination. Many non genetic researches
females remain and breed in the group of
have been carried out in these long tailed
their birth. The females compose a nuclear
macaques (Loudon et al., 2006; Lissa et
members of the social group (Joly, 1985;
al., 2008; Lane et al., 2010;, Schilaci et
Napier-Napier,
Perwitasari-
al., 2010; Brotcorne et al., 2011; Fuentes
Farajallah et al., 2004). Different from the
et al., 2011; Suarjana & Asmara, 2012).
female, male macaques will not stay firmly
The research about genetic variation in
in natal group, but migrate to other group
long tailed macaque population in Bali has
during their growing up period. The male
been conducted by Kawamoto et al.
migration would be a genetic bridge for
(1994) using blood protein marker. Other
entering the source of genetic variation to
research
the new population or group. This gene
microsatellite
1985;
62
like
i.e.
Sangeh,
Ubud,
characterization
locus
on
Alas
of
somatic
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
Vol 2 No 2: 61-70
13 samples.
chromosome has been carried out by
Wandia (2003, 2004a, 2004b), and Rell et
The total DNA was extracted using
al. (2013). However, there was no study
QIAamp® DNA Blood Kits according to
that exploring the genetic variation using
company protocol (Qiagen, 2007). Three
molecular marker on Y chromosome of the
microsatellite loci on Y chromosome were
long tailed macaques. This research aimed
examined
to characterize the microsatellite loci on Y
human microsatellite primer (DYS390,
chromosome of long tailed macaque
DYS391 and DYS393). The loci were
populations
amplified by polymerase chain reaction
in
the
Island
of
Bali,
their
tecnique,
polymorphism
and
each
using
Indonesia. The polymorphic information
(PCR)
reaction
of loci is very important before using them
contained 4 mM MgCl2; dNTPs each 0.16
as molecular marker for assessing the
mM; a pair of primers each 0.4 mM; 0.7 U
DNA Polymerase; 1.25 l PCR buffer
genetic variation within and between
(10x), and 1 l template DNA. Pure water
populations.
was added to make the final volume of
reaction 12.5 l (Hillis et al., 1996). PCR
MATERIALS AND METHOD
for
DYS390
locus
was:
Pre
PCR:
A total of ninety nine male long tailed
denaturation (94o C) for 3 minutes; PCR:
macaque blood samples was subjected for
denaturation (94o C) 35 seconds, annealing
DNA extraction. The blood samples were
(50o C) 35 seconds, and elongation (72o C)
collected in the year 2010-2011 by
35 seconds; post PCR: elongation (72o C)
research team of Primate Research Center
5 minutes. PCR was replicated 30 cycles
Udayana University. The macaques were
using Applied Biosystems 2720 Thermal
anaesthetized with the combination of
Cycler. The PCR for DYS391 and
Ketamin HCl (dose 10 mg/kg body
DYS393 was the same as those of
weight) and Xylazin with ratio 5:1, and the
DYS390 with the exception of annealing
drug was injected using blow pipe. These
temperature at 54o C. The PCR product
samples
local
was separated by 7% PAGE in 1x TBE
populations in Bali Island, namely Sangeh
buffer and run at 125 Volt for 90 minutes.
8 samples, Uluwatu 6 samples, Alas
The allelic variation was developed using
Kedaton 11 samples, Ubud 11 samples,
silver staining, and the length of allele was
Bukit Gumang 16 samples, Pulaki 23
measured by aligning the allelic band with
samples, Bedugul 11 samples, and Mekori
the bands of 100 base pairs ladder of
originated
from
eight
63
Wandia et al.
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
molecular marker. The polymorphism of
length of the alleles was 282 base pairs
loci was analyzed using Alrequin Ver 3.5
and 292 base pairs. This locus was
soft ware (Excoffier & Lischer, 2010).
polymorphic, however, the two alleles
were distributed oddly.
The allele 292
(DYS391292) was found in all populations,
RESULTS AND DISCCUSIONS
in contrary, the allele 282 (DYS391282)
Results
was just found in population of Pulaki.
Distribution of alleles and haplotypes
Two other loci (DYS390 and DYS393)
were monomorphic as long as these loci
The results showed that two alleles
just had one allele (Table 1).
were found in DYS391 locus which the
Table 1. Allele Distribution of the Microsatellite Loci on Y Chromosome of Long Tailed
Macaque Populations in Bali Island
Relative Frequencies of allele in sampling site
No
Loci
Allele
Total
PL
n=23
BD
MK
n=11 n=13
SG
n=8
AK
n=11
UW
n=6
UB
BG
Bali
n=11 n=16 n=99
1
DYS390 DYS390240
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
2
DYS391 DYS391292
0.91
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.98
DYS391282
0.09
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.02
DYS393 DYS393272
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
3
Mapping of the three loci in one individual of Y chromosome will compose the
haplotype. Genetic analysis showed that there were two kinds of haplotype, in which, one
of them was only distributed to population of Pulaki (Table 2).
Table 2. Haplotype distribution of Y Chromosome of Long Tailed Macaque Populations
in Bali Island
Relative frequencies of haplotype in population
Haplotype
hp composition
1
2
Total
PL
BD
MK
n=23 n=11 n=13
SG
n=8
AK
n=11
UW
n=6
UB
BG
Bali
n=11 n=16 n=99
hp240:292;272
0.91
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.98
hp240;282; 272
0.09
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.02
64
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
Vol 2 No 2: 61-70
Locus and Haplotype Diversities of Y Chromosome of Long Tailed Macaque
Populations in Bali Island
Locus and haplotype diversities of Y chromosome of long tailed macaque
populations were very low, or even zero in most populations (Table 3). It has some thing
to do with the monomorphic condition of the loci. Polymorphic condition of DYS391
locus in population of Pulaki has contributed to the value of haplotype diversity
(hd=0.17).
Tabel 3 Locus and Haplotype Diversities of Y Chromosome of Long Tailed Macaque
Populations in Bali Island
Diversity in populations
Total
Source
PL
BD
MK
n=23 n=11 n=13
SG
n=8
AK
n=11
UW
n=6
UB
BG
Bali
n=11 n=16 n=99
DYS390 locus
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
DYS391 locus
0.17
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.04
DYS393 locus
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Haplotype (hd)
0.17
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.04
replication and due to the effective number
Disccusions
A Locus polymorphism level is
of the variation source is a fourth apart of
supplied by the number of genetic
those of the somatic chromosome, this
variation sources in a population. The
genetic marker will reach a fixation in
variation of mtDNA genetic marker comes
shorter evolutionary time in a population
from one source, mtDNA of the female
compare to the genetic marker of somatic
(Walace et al., 1999; Ingman et al., 2000;
chromosome. The resulth of this research
Bowels et al., 2007), likewise, the
exibited three allales of the four alleles
variation of a genetic marker of Y
found were fixed or nearly fixed in all
chromosome
populations. Nearly fixation state of those
just comes from
Y
chromosome beared by the male (Evans et
alleles may associate with
al., 2010; Rovie-Rian et al., 2013). As
recombination during replication and low
long as a genetic marker of Y chromosome
effective number of variation source as
does not undergo a recombination during
describes above. There was only one allel,
65
no DNA
Wandia et al.
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
DYS391282, had low frequency and just
populations in Bali Island was lower than
found in population of Pulaki (Tabel 1).
those
This may reflect a new mutation of allele,
populations in Lombok Island. The botlle
therefore, the male bearing the allele has
neck effect may decrease severely the total
not had enough chances to distribute the
number of the population members, and so
allele through mating with a female within
do the number of the male. While this
a population as well as migrating to other
preliminary
populations.
microsatellite loci on Y chromosome and
of
the
long
tailed
resarch
macaque
used
three
The low haplotype diversities are not
only one of them was polimorphic, the
only affected by the total number of
data may not express the real condition. It
geneticaly different male in population,
would
but also the number of loci used and the
individual
polimorphism of the loci (Perwitasari-
populations.
Farajallah et al., 2004). Low haplotype
pertaining to the evolutionary history of
diversities of Y chromosome may reflects
male macaques in Bali Island, a further
to a few genetically different male of long
research
taild macaques in wild in Bali Island. The
microsatellite loci on Y chromosome is
long tailed macaques in Bali Island were
needed. Moreover, a broaden sampling
originated from Java Island and they
sites
migrated during the two islands unite to be
including population in Java Isalnd and
a part of Sunda Selft (Eudey 1980; Fooden
Lombok
1995). However, there is no a clear
evolutionary history of male macaque in
information about the
of
South Archipelago of Indonesia. However,
migration events and the total number of
this research has succeeded to characterize
different populations had migrated. This
three microsatellite loci on Y chromosome
lack of information brings to the difficulty
at native populations of long tailed
to justify the male number of long tailed
macaque in Bali Island.
frequencies
be
weak
both
(population)
Island
differentiating
within
To
using
in
and
better
a
understand
more
may
to
between
be
polimorfic
obligated
elucidate
the
macaques as the founder population in
Bali Island in the past. Kawamoto et al.
CONCLUSION
(1984) stated that the long tailed macaques
populations in Bali Island had suffered
Based on the result of the research, it
from a bottle neck effect. It was based on
can be concluded that each of DYS390 and
the
DYS393 loci has one allele, so, these two
blood protein data that the genetic
variation
of
long
tailed
macaque
loci are monomorphic in male long tailed
66
Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014
Vol 2 No 2: 61-70
macaques in Bali Island. On the other
Indonesia. In Monkeys on the Edge.
hand, DYS391 locus that having two
Ecology and Management of Long
alleles is polymorphic. The locus of
Tailed Macaques and Their Interface
DYS391 could be used in the study of
with Humans. Editors M.D. Gumert,
genetic differentiation between male long
A.
tailed macaque populations in Bali Island,
Cambridge University Press, Pp 180-
but not the two others.
182.
Fuentes,
and
L.Jones-Engel.
Eudey AA. 1980. Pleistocene glacial
phenomena and the evolution of Asian
ACKNOWLEDGEMENT
macaques. In The Macaques. Studies
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the President
of
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Udayana University for grand approval on
Edited by D.G. Lindburg. :52-83.
this research with the Contract No:
Evans BJ, Pin L, Melnick DJ, Wright SI.
21.4/UN14/KU.03.04/2012, on the date of
2010.
May, 16 2012 and the Contract No:
macaque monkeys: Implications for
174.17/UN14.2/PNL.01.03.00/2013,
effective population size and dispersal
on
the date of May,16 2013.
Sex-linked
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July 2010). Copyright _ 2010 by the
Genetics Society of America DOI:
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