REGENERASI KALUS SAGU ( metroxylon so ) PADA BERBAGAI KOMBINASI KONSENTRASI AUKSIN DAN SITOKININ MELALUI TEKNIK KULTUR JARINGAN.
REGENERASI KALUS SAGU ( metroxylon so ) PADA BERBAGAI KOMBINASI
KONSENTRASI AUKSIN DAN SITOKININ MELALUI TEKNIK KULTUR JARINGAN
Reni Mayerni, Media Isma Nugraha, Istino Ferita
Nomor Kontrak : 05/LP-UA/SPP/DPP
ABSTRAK
An experiment on the callus regeneration of sago palm different combination of
auxin and citocinin concentration by in –vitro was carried out at plant tissue culture lab.
Of departement of Agronomy, Faculty of Agricultture, Andalas university, padang From
Juni to Oktober 2001. The object of experiment was to obtain the best combination of NAA
and BAP for Promoting callus generation of sago palm culture in- vitro. The experiments
used the completely randomised design with eight packages of plant growth regulator i. E.
A : 0,25 ppm NAA + 1,75 ppm BAP; B. 0,25 ppm NAA + 3,00 ppm BAP; C. 0,5 ppmNAA +
1,75 ppm BAP: D. 0,5 ppm NAA + 3,00 ppm BAP; E. 0,25 ppm NAA 1,75 ppm BAP + 0,1
ppm + 0,1 ppm cinnetin; F. 0,25 ppm NAA + 3,00 ppm BAP + 0,1 ppm cinetin; G. 0,5 ppm
NAA + 1,75 ppm BAP + 0,1 ppm BAP cinetin; H. 0,5 ppm NAA 3,00 ppm BAP + 0,1 ppm
cinetin.
Result of this research indicate that auxin and citocinin gave no effecttoall of
research observation parameter because no callaus induction tissue enlarged, developed
and turned green on the media, but no callus induction or axilliary bud outgrowth has been
obtained to date.
KONSENTRASI AUKSIN DAN SITOKININ MELALUI TEKNIK KULTUR JARINGAN
Reni Mayerni, Media Isma Nugraha, Istino Ferita
Nomor Kontrak : 05/LP-UA/SPP/DPP
ABSTRAK
An experiment on the callus regeneration of sago palm different combination of
auxin and citocinin concentration by in –vitro was carried out at plant tissue culture lab.
Of departement of Agronomy, Faculty of Agricultture, Andalas university, padang From
Juni to Oktober 2001. The object of experiment was to obtain the best combination of NAA
and BAP for Promoting callus generation of sago palm culture in- vitro. The experiments
used the completely randomised design with eight packages of plant growth regulator i. E.
A : 0,25 ppm NAA + 1,75 ppm BAP; B. 0,25 ppm NAA + 3,00 ppm BAP; C. 0,5 ppmNAA +
1,75 ppm BAP: D. 0,5 ppm NAA + 3,00 ppm BAP; E. 0,25 ppm NAA 1,75 ppm BAP + 0,1
ppm + 0,1 ppm cinnetin; F. 0,25 ppm NAA + 3,00 ppm BAP + 0,1 ppm cinetin; G. 0,5 ppm
NAA + 1,75 ppm BAP + 0,1 ppm BAP cinetin; H. 0,5 ppm NAA 3,00 ppm BAP + 0,1 ppm
cinetin.
Result of this research indicate that auxin and citocinin gave no effecttoall of
research observation parameter because no callaus induction tissue enlarged, developed
and turned green on the media, but no callus induction or axilliary bud outgrowth has been
obtained to date.