Poorly Soluble Drugs and Protein
\
-
Proceeding
Thelohternadonal
I
T
on Pharmaceutics & Pharmaceutical Sciences
Drug Delivery Systems:
From Drug-Discovery, Pre-formulation, Formulation and
Poorly Soluble Drugs and Protein
-1a-fl
:
--.-
Proceeding
*
fl re I lnternational Gorfercme
on Pharmaceutics & Pharmaceutical Sciences
Published and Organized by
Faculty of Pharmacy
Universitas Airlangga
Surabayalndonesla
2014
h-a
The l"t I nternationa I Conference on Pharmaceutics
& Pharmaceutical Sciences Proceedings
ISBN : 97&602-72333-G.o
(Lefter of Natlonal ISBN Agency No. 4127E.8/p/03.2015 Date 18 March 2015)
lst edltion Proceedlng
Publlshed by:
Faculty of Pharmacy UniveBitas Airlangga
Surabaya, lndonesia
Addr€ss:
Kampus B rl. Dharmawangsa Dalam
Surabaya 60286
Phone +52 31 5033710
Fax +62 31 5020514
Website: www.icpps2ol4.com or www.ff.
Email: [email protected]
ISBN i? 6 -
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PREFACE
From Chairman
It is our
pleasure to present you the proceedings of The 1st lntemational Conference on
Pharmaceutics and Pharmaceutical Sciences (ICPPS) oBanized by The Faculty of Pharmacy
Universitas Airlangga Surabaya lndonesia.
The proceeding was produced based on papers and posters presented at The 1st lntemational
Confurence on Pharmaceutics and Pharmaceutical Sciences (ICPPS), held in Sunbaya, lndonesia, 1415 November2014.
The proceeding clearly reflects broad interest, from the participants thatcoming fmm allaround the
world.
The papers presented were pharmaceutics and biopharmaceutics; requirements on how to e\raluate
molecules in discovery and their appropriateness for selection as potential candidate; their
development in context of challenges and benefits, together with associated time and cost
implications and also requirements to progressthrough pre{linieland clinical.
ln this an opportunity, I would like to express my appreciation to the editorialteam ofthe proceeding
who hrve been workin8 hard to review manuscripts, and makingthe first edition ofthis proceedin8
be possible.
I would like also to thanks to all invited speakers and presenters who participated in The lst
lnternational Conference on Pharmaceutics and Pharmaceutical Sciences (ICPPS) and your
mntribution to this proceeding.
Finally,
I hope this proceeding will give contribution to the Pharmaceutics and Pharmaceutical
Sciences research.
Chairman,
Dra. Esti Hendradi, MSl,, Ph.D., Apt
COMMITTEE
Advisor
Chairman
: Dean of Faculty of Pharmary Universitas Airlangga
: Dra. Esti Hendradi, M.Si., Ph.D, Apt.
Editor
: Dr.
Wouter U. Hinrichs (Netherlands)
Prof. Kozo Takayama (Japan)
Mutalik (lndia)
Dr. Nuttakorn Baisaeng (Thailand)
Prof. Dr. Widji Soeratri, DEA (lndonesia)
Dr. Srinivas
Committee
Prof. Dr. Widji Soeratri, DEA, Apt.
Prof. Dr. Siswandono, MS., Apt.
Prof .Dr. Tutuk Budiati, MS., Apt.
Prof. Dr. rer.nat. Muhammad Yuwono, MS., Apt,
Drs. Hadi Poerwonq MSc.,Ph.D, Apt.
Drs. Marcellino Rudyanto, MSi., Ph.D, Apt.
Drs. Bambang Widjaja, M.Si, Apt.
Drs. Sugiyartonq MS, Apt.
Dra.Tutiek Purwanti, MSi, Apt.
Dra.Tristiana Erawati, MSi, Apt.
Dra.Retno Sari, MSc, Apt.
Dra.Dewi lsadiartuti, MSi, Apt.
Dra.Noorma Rosita, MSi, Apt.
Dr. Dwi Setyawan, SSi, MSi., Apt.
Dr. Riesta Primaharinastiti, SSi, MSi, Apt.
Dewi Melani Hariyadi, SSi, M.Phil., Ph.D, Apt.
Helmy Yusuf, SSi, MSc., Ph.D, Apt.
Dr. rer. nat. Maria Lucia Ardhani Dwi Lestari, SSi,MPharmSci., Apt.
Melanny lka Sulistyowati, SFarm, M.Sc, Apt.
Febri Annuryanti, SFarm,M.Sc, Apt.
M. Faris Adrianto, MFarm, Apt.
Dini Retnowati, SFarm,Apt.
Abhimata B SFarm, Apt.
of CONTENT
TABI,T
Preface from Chairman
Committee
tl
Table of Contents
iii
Author lndex
iii
AUTHOR INDEX
COMPARISON OF SODIUM STARCH GLYCOLATE AND CROSSCARMELTOSE SOOIUM AS
SUPERDISINTEGRANT IN MEFENAMIC ACID FAST DISINTEGRATING TABI.ET
Adeltrudls Adelsa D, Oktavia Eka Pusplta, Amalla Ayuningtyas, Marulita lsadora
1
STUDY EXPRESSION OF HUMAN ERYTHROPOIETIN EXPRESSSION IN MAMMAUAN CELL
Adl samoso, Popl Hadiwisnuwardhani, Yana Rublana, Yulalka Romadhani, Endah Puji
Septisetyanl, Dyanin$yas D,P, Putri
4
ANTIOXIDANT STABILITY ASSAY OF ATPHA TOCOPHERYI- ACETATE IN SOLID LIPID NANOPARTICLE
SYSTEM
AnSEie
(t,PlD eqSE BEESWAX AND MONOSTEABIC 6LISEBYL 50:50)
wldhi, Noorma Rosita, lMdii Soe6tri
..............,.....
A BIOACTIVE SOYINE HYDROXYAPATITrcETATIN
..,............,...,.,... 8
IMPLANT FOR IN VITRO GENTAMICIN RELEASE
13
Anlek s€dya Budiatn, M. zainuddin, Junaidi Xhotib, Diah Himawati
EFFECT OF COMPARISON SURFA TA T AND @SURFA TANT IN WATER/OII, MICROEMUTSION IN
REEASE OF OVALBUMIN Microemulsion Water/Oil with Surfactant (Span SGTween E0) :
t'"
,'t,
.nO
Cosu rfactant (Ethanol) =S'1
Anisa Rizki Amalla, Riesta Primahadnasbti, Esti Hendradi
ANALYSIS OF
MYcotlc
ACIDS CTEAVAGE PRODUCT OF
18
MycoDocterium tuberculosis BY GAS
CHROMATOGRAPHY-FIAME IONIZATION DETECTOR
Asrl Darmawati, Deby Kusumaningrum, lsnaeni, Muhamad Zainuddin
2l
PERIPI"ASMIC EXPRESSION OF GENE ENCODING ANT!EGFRvlll SINGLE-CHAIN VARIABLE FRAGMENT
ANTIBODY USING PelB TEADER SEQUENCETN ES6HER|CHIA cOLl
Kartika Sari Dewl, Debble Sofie Retnoringrum, Catur Riani, Asrul Muhamad Fuad
24
IN VIVO ANTIMALARIAL ACTIVIry OF ETHANOL EXTRACT AND ETHYT ACETATE FRACTION OF
Aledryon serrotus LEAVES ON Prasmodrum berghei INFECTED MICE
AW Widyawaruyand, Uswatun Khasanah, Lldya Tumewu, Hilkatul llmi, Achmad Fuad Haffd,
...........'.............. :lO
lndah 5 Tantular ...
PROFILE OF COMMUNITY PHARMACISTS KNOWLEDGE IN PATIENT ASSESSMENT WITH INFIUENZA
SYMPTOMS AND ITS PRODUCTS
Azza Faturrohmah, Atle Sullstyetlni, Ana Yuda
33
soLUBtLtTy AND DTSSOLUTTON SrUDY OF KETOPROFEN
lNcLUslON COMPLEX (Prepared by Kneading Method)
Bambant Wdrala, Achmad RadJaram, Arafah Zulhana
-
HIDROXYPROPYL-p-CYCLODETTRIN
,,.....
,..........'.......... 37
FORMUTATION AND STABILITY TESTING OF MELOXICAM SOLID DISPERSION GEL
Budlpntlwl Wlsudyantnlsih, lnka Dewl Nur Angraraini, Fersiya Wardani
EFFECT OF MENTHOL AS PENEIRATION ENHANCER TO DICLOFENAC
40
SODIUM MEMBRANE-ryPED
TRANSDERMAT PATCH CHARACIERIZANON
Destrla lndah Sarl, Esd Hendradl, Junaldl Khodb
43
PHYSICAL CHARACTERISTICS AND RELEASE STUDY OF OVATBUMIN FROM ALGINATE MICROSPHERES
PREPARED BY DIFFERENT CONCENTRATION OF ALGINAIT AND 8aCI, USING AEROSOLIZATION
TECHNIQUE
Dewl Melanl liarlyadl, Trlsdana
El"yrti, sisilia Ermavahyuningtyas
45
MUCOADHESIVE TABLET OF ETHANOLIC EXTRACT OF SAMBILoTo (Andrographis paniculata)
AS ANTIDIABETIC USING CHITOSAN
Dhadhang wahyu Xumlawan, Henlng Pratiwi, and Uryga lkaditya
50
PHYSICAT INTERACTION STUDY OF IBUPROFEN.STEARIC ACID BINARY MIXTURE
Olalent Putrl Paramlta, Dwl Setyauran, Dewi lsadiartuti
....
.,.,..............,.... 59
MoLEcUtAR MoDELING AND SYNTHESIS oF 1-(3,+Dichlorobenzoyl)-1,3-dimethylurea
DIan Agun8 Pangarlbowo, Slswandono, BambangTri Purwanto
63
EXPRESSION OF RECOMBINANT HUMAN GRANULOCYTE{OLONY STIMUTATING FACTOR WITHIN
PERIPLASMIC COMPARTMENT OF Esc,}ench,o
coli USING PeIB LEADER PEPTIDE
Dlrn Fitrh Atxr3tvantl, Aerul Muhamad Fuad ,--**----.-
..,,.,,..,...,..,,....*
EVALUATION OF ANTIHYPERURICEMIC ACTIVITY FROM BULBS OF BAWANG TIWAI lEleutherine
BY lN VITRO ANO lN VIVO STUDIES
polmifolld (Ll Merr.,
Dlan Radh laksmltawaq Rlnlnta Firdaus, \tulinda, Mediana Asdka
72
lFrogorio x ononosso Duch.) AND SfARFRUff {Averrhod carombolo L.} USING ABTS
SCAVENGING METHOD
Dhna Sedahwaty, lndira Natalia
Timang
FREE
RADICAI
..,.....,...,...,.,.. 76
ENHANCEMENT OF SOLUBILrIY AND DISSOLUTION ATORVASTATIN BY MICROCRYSTATLIZATION
METHOO
Dollh Gorall, Yoga Windu wa.dhana, Ronny Tandela
79
lrv yrIRO ANTIMAIARIAL ACrIVITY OF DICHLOROMETHANE SUB-FRACTION OF Eucalyptus globulus
L, Stem AGAINST P,osmodium folcipdrum
Ells Suwarnl, Achmad Fuad Hafid, Aty WidyawarutBnti
86
Arcongelisid llavo INCREASES RATS' LEUKOCYTES BUT HAS BIPHASIC EFFECT ON RATS' LYMPHOCYTE
Endah Puspitasari, Evi Umayah Ulfa, Vlta Arlatl, Mohammad SuhhonHabibi
E9
IN V]TRO ANTIMAIARIAL ACTIVITY OF CHLOROFORM SUBFRACTION OF sALAM BADAK LEAVES
lAcme na ocu m i noti ssi mol
Erna Caf,vanlwslh,
CHARACTERIZATION
A&Drd Fud Htfd, Aly l,yidyawaruyanf ....,.,........................................92
OF DOSAGE FORM AND PENETRATION DICLOFENAC SODIUM WITH
MICROEMULSION SYSTEM lN HPMC 4000 GEL BASE (Micmemulsion
Span 80 - Tween 80 : Cosurfactan Ethanol 96% = 5:1)
WO with rasio
use of surfactant
Estl Hendndl, Tudek Purwar*i, lGrina Wahyu lrawad
95
CONSTRUCTION AND VALIDATION OF THE STRUCTURE.BASED VIRTUAL SCREENING PROTOCOts
WITH PDB CODE OF 3LN1 TO DISCOVER CYCLOOXYGENASE.2 INHIBITORS
Mumpunl
E,
Nurrcchmad A, Pranowo HD, renie UA lstyastono EP
99
VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF ASPIRIN IN RABBIT
PIAsMA : APLICATION TO BIOAVAILABIIITY STUDY OF ASPIRIN MICROCAPSULE lN RASSIT
toz
Falzatun, Novl yantih, Teguh lman Saputra
EFFECT OF COMPARISON OF SURFACTANT AND COSURFACTANT WO MICROEMULSION OVALBUMIN
WITH SOYBEAN OILTO PHYSICOCHEMICAL CHARACfERIZATION (w/o Microemulsion with Surfactant
Span 8G Tween 80 : Cosurfactant Ethanol96% = 5:1; 6:1 and 7:1)
Farlda Mutlara Sarl, Rlesta Prlmaharlnasttd, Esd Hendradi
105
pH INFLUENCE IN DESATTING PROCESS OF CRUDE PERTUSSIS TOXIN (PT) AND FILAMENTOUS
HEMAGGLUTININ (FHA) PURIFICATION FROM Bordetello pertussis BY SEPHADEX G-25 COLUMN
CHROMATOGRAPHY
Faris Adrlanto, Esd Hendradl,
tlenl ,{uElny, lsnaeni
108
SEPARATION OF COSMETIC PRESERVATIVES USING SILICA-BASED MONOLITHIC COTUMN
Febri Annuryanti, Riesta Primaharinastiti, Moch. \tuwono
111
PREPARATION AND CHARACTERIZATION OF TELMISARTAN.CITRIC ACID CO.CRYSTAL
Fikri Alatas, Hesdary Ratih,
Sundani umno So€wandhie
114
PATIENTS' AND CAREGIVERS' LIQUID MEDICATION ADMINISTRATION ERRORS
Gusti Noorrizka vemnika Achma4 Gesnita Nugrahenl
.,.,
,...,.......,......,.... 117
THE POTENCY OF CANARIUM OIL (Conarium indicuml AS A MATERIAL FOR STRUCTURED LIPID
PRODUCTION
Hamidah Rahman, Johnner
P
Sitompul, lfusnandar Angtadiredja, Tutus Gusdlnar .,........,.,.. 121
EFFECT OF TREHALOSE ON THERMAL PROPERTIES OF PHOSPHOTIPID-DDA AND TPGS MIXTURES
Helmy Yusuf
.......,.
,.,...,..,.....,..,,.,,, 124
PEEPABATION AND CHABACTEB'ZATION OF FLUKONAZOLE.9.CYCLODEXTRIN INCLUSION COMPLEXES
Hestiary Ratlh, Flkrl Alatas, Erin xarlina
L27
ISOLATION AND IDENTIFICATION OF ANIIOXIDAI{T COMPOUND BY BIOPRODUCTION OF
ENDOPHYTIC FUNGI OF TURMERIC (Curcuma longo L.l ISOLATE CL.SMl.RF11
Hindra Rahmawati, Bustanussalam, Partomuan Simaniuntak
130
MODIFICATION PROCESS OF NATURAT CASSAVA STARCH : THE STUDY OF CHARACTERISTICS AND
PHYSICAT PROPERTIES
Prasetia, Jemmy A, C,l.S, Arlsanti, I{.P.P,A. Dewl,
6-A.i. Astutf, l{,W,il Yulianiqsih, I M.A.G.
Wirasuta
133
ORUG USE PROFILE OF DIABETIC PATIENTS IN EAST SURABAYA PRIMARY HEALTH CARE
I ilyoman
wijata, A:ra Fatunohmah, Ana Yuda, Mufartlha,
Hlkmah Prasasti
t'1,
Tesa Geoyanl santoso, Dlna xartlka,
Whanni Wido Agustin
136
GLYCINE MAX DETAM II VARIEry AS PREVET{TIVE AND CURATIVE ORGAN DAMAGE DUE TO
EXPOSURE TO ,LEAD (Pb)
Rlka Yulla, Sylvan Septian R6sandy, Gusd AW Putu Puspikaryani, I Putu Atus Yulyastr"wan, D€wa
lq2
AW Kusuma Dewi
AN ACTIVITY TEST OF MATOA LEAVES EXTRACT AS HEART RATE FREQUENCY REDUCTION WITH
ADRENATINE INDUCTION
lk
t4
Purwldy.nlntrum, Elln Yulinah Sukandar, lda Fidrianny
EFFORT TO REDUCE COMPRESSIBITITY OF RAMIPRILTHROUGH CRYSTAT ENGINEERING
lnd6, Smdanl
N
So€sendhi
..-.,,.,...*..*
1|7
IN VITRO ALPHA-GLUCOSIDASE INHIBITORY ACNVIY OF ETHANOLIC LEAF EXTRACT AND FRACNONS
Rouvofio seryenliino (1.) Benth. ex Kurz
Julle Anne D. Bolaftos, lvan L Lawag
OF
.,
.........,............ 15o
PERIPTAsMIC EXPRESSIOI{ OF GENE ENCODING AI{TFEGFRVIII SIN6I.E{HAIN VARIABI.E FRAGMENT
ANTIEO0Y USING PelB LEADER SEQUENCEIN Escherichio coli
(artika Sari Oewi, Debbie Sofie Retnoningrum,catur Riani, Asrul Muhamad Fuad ...,.,,....,. 153
CHARACTERIZATION
AND LD- VALUE DETERMINATION OF
1,s-bis(3'-ethoxy{'-hydroxyphenyl}-1,4-pentadiene.3-one
testarl Rahayu, Septian, Esti
(EHP)
Mumpuni,
,..,..,.,.,........ 159
DEVELOPMENT OF MELOXICAM TRANSDERMAT MATRIX TYPE PATCH USING
POLWINYTPYRROI-IDONE, HYOROXYPROPYL METHYTCELTULOSE, AND ETHYI- CELI.ULOSE
COMBINATION
t62
tldya Am.llana, Monica lwud, Selly Rlo
ANTIHEPATITIS C VIRUS ACTIVITY SCREENIN6 ON
Harpul,io orboreo EXTRACTS AND ISOLATED
COMPOUND
Lldya Tumewu, Evhy Apryani, Mei Ria Sant, Tutik Srl Wahyuni, Adita Ayu Permanasa4
Myma Adlantl, Chle Aoki, Aty Widyawarufanti, Adrmad Fuad Hafd, Maria lnge Lusida,
......,.....,....... 165
Hotta
Soetjipto, Hak
HPLc METHoD PRECISION TO ASSAY OF A-MANGOSTIN lN Mangosteen (6orc,nia mongostano L.l
FRUIT RIND EXTRACT FORMULATED IN ORAL SOLUTION
Liliek Nurhidafat, Siti Sofiah, Ros Sumarny, Xevin
Caesar.
....,......,,....... 168
;qiPARATION AND CHARACTERIZATION OF NARINGENIN.LOADED CHITOSAN NANOPARTICLES FOR
OiEMOPREVENTION
Lira lMnarti, Lusla Oktora Ruma Kumala Sari
t70
IE'.ATIONSHIP OF KNOWLEDGE AND PATIENT BEHAVIOR ON SELF MEDICATION PIROXICAM
i Sludies of Pharmacy in Sukun District, Malang City)
Ljza Pristianty, Reshtia Erlana Putd, Hldayah Rachmawatl
t7t
iFFECT OF CHRONIC USE OF ENEBGY DBI'IX ON XIDNEY
tlahardlan Rahmadi, Zamtotul lzzah, Mareta Rindant A, Aniek Setya
SCREENING OF SURFACE MODIFIERS TO PRODUCE
GUIDANCE
Mada Lucia Ardhani
B,
Suhadono ........... 176
SIABE NANOSUSPENSION : A GENERAI
llxi l€stari ..,......
............,......... 179
,EVETOPMENT OF SIMPLE POLYPHENOL SENSOR BASED ON SODIUM META PERIODATE AND 3.
METHYT-2.BENZOTHIAZOLINONE HYDRAZONE FOR COFFEE SAMPLES
t
och. Amrun
Hidayat
indya Pusp'rtaningtyas, Agus Abdul Ganl, Bambant Kuswandi ...,.. 181
VALIDATION OF AN HPLC ANALYTICAL METHOD FOR DETERMINATION OF LEVOFLOXACIN IN
OPHTHALM IC PREPARATIONS
Mochamad Yuwono, Riesla Primaharlnastiti, Ateng Teguh Wbrdoyo
VAUDATION OF SPECTROPHOTOMETRIC
IN TABLET DOSAGE FORM
Nia Xrlsfnlntrum, Diah Yuli ParyEsti
184
M€THOD FOR ESTIMATION OF EPERISONE HYDROCHLORIDE
...
..,...,.......,..... 187
ANNOSTEOPOROTIC ACNV|TY OF 96% ETHANOUC EXTRACTS OF ABELMOSCHUS MANIHOT LMEDIK
LEAVES AND EXERCISE ON INCREASING BONE DENSITY OF FEMALE MICES FEMORALTRABECULAR
190
Niliestria Ayu Faramitha Sholikhah
EFFECT OF {YCTODETTRIN ON sPF VALUE AND INHIBITION OF KO.,IC ACIDSTYROSINASE ACTIVTTY
IN VANIsHING CREAM BASE FORMUTATION (ON SUNSCREEN PRODUCT CONTAINED OXYBENZONE)
Noorma Rosita, Diana, Diana winarlta, Tristiana Erawati, Wldji sroeratrl
193
ANTIMICROBIAL ACTIVITY OF LACTOBACILLI PROBIOTIC MILK AND GUAVA I FAF ETHANOLIC EXTRACT
(Psidium guajava) COMBINATION AGAINST BACTERIAI CAUSE OF DIARRHEA
197
ur Putri Ranti,lsnaeni, Juniar Moechtar, Febri Annunranti
THE INTTUENCES OF PARTICTE SIZE AND SHAPE ON ZEIA POTENTIAL OF COENZYME Q1O
NANOSUSPENSION
Nuttakom
Baisaeng
.......'.'.'....'... 200
SYNTHESIS, MOIECUTAR DOCKING, AND ANTITUMOS ACTIVITY OF
Dimethylurea AGAINST HUMAN BREAST CANCER CELL LINE (MCF-7)'\I,NLDibENZOYI-N,NL
..".'......'........ 203
Nuzul walryuning Diyah
.........,..,....,....
EXPRESSION OF ANII-EGFRVIII SINGLE CHAIN FRAGMENT ANTIBODY (SCFV)ON THE SURFACE OF
PICHIA PASTORIS
hlka vio&rfi,
Asrut Muh.m.d Fuad,
tuftarsono
.'...'..'.'.---.'.
z)5
SUBCLONING OF csf3syn (COLONY SnMULATING FACTOR-3) GENE INTO pGAPZ? AND
TRANSFORMATION OF RECOMBINANT VECTOR INTO PICHIA PASTORIS
Prety lhda Arfia, Asrul Muhamad
Fuad
,...............,...212
THE USE OF PERICARP MANGOSTEEN (Garcifiia mangostana
CREAM-TYPE O
L) EXIRACr lN
FORMUTATION OF
/W
Rahmah Elfiyanl, ilaniek setladi Radjab, Mla Sadta soffan ,.,.,.....,.,...,,.........,...,...,,..,...,... 218
CHITOSAN BASED PARTICULATE CARRIER OF DITIRPENE LACTON OF SAMBILOTO PREPARED BY IONIC
GELATION-SPRAY DRYING :EFFECT oF STIRRING RATE AND NOZI..E DIAMETER
Retno Sad l-run Suhartanti, Ihxt Setyawan, Esd Hendrrdl, WUI Soeratrt.-.--
*--.*---.....2X2
GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD VALIDATION FOR PESTIODES RESIDUES
ANALYSIS IN FOOD USING QUEChERS KIT
22s
Rlesta Prlmaharinastid, Setyo Prlhatinlngty'.s, Mochammad Yuwono
CHARACIERIZATION OF PARACETAMOL ORAUY DISINTEGRATING TABLET USING GEI.ATIN 1% ANO 2%
AS BINDER AND POLYPTASDONE XL-1O 10% AS DISINIEGRANT (Prepared by Freeze Drying Method)
Roisah Nawadla, D{ri Setyawan. Bambant wldjaja
230
ANTIox|DANT STUDY oF COSOTVENT SOLUTION OF MANGOSrEEN (Garcinia manBostana L) RIND
EXTRACT IN RATS BY USING MDA PARAMETER
Ros Sumamy, Liliek ,{urhldafad, Yat Sumlyati, Fransiska Dlana Sanii...........,......,.,...,.,.,.,. 233
SCREENING OF SELECTED PHILIPPINE ROOT CROPS FOR ?-Glucosidase INHIBITION
SaEh Jane
S.
Almazora, lrran L Lawat
...
............,...... 236
NIOSOME EMULGEL FORMUTATION AND STABLITY TEST OF CINNAMON (Gnnamomum burmanii
Nees & Th. Nees) Eark ETHANOIIC EXfRACf AS ANTIoXIDANT
Sasand Dariianto, Fldrhni |rda, Widhita
Pff
239
THEOPHYLTINE RELEASE FROM SUSTAINED RELEASE TABLET USING LACTOSA AND PVP K3O A5 A
CHANETIING AGENT
Sugiyartono, Retno sari, Agus Syamsur Rlral, TriMuhanl, Atustin Maharani
242
EFFORTS TO PRODUCE 1-(EENZOYLOXY)UREA
AS ANTICANCER DRUG CANDIDATE
suko Hardjono
245
DEVELOPMENT OF PEGYIAtEd RAPAMYCIN LITHOCHOTIC ACID MICELLE FOR CANCER THERAPY
Ann Tapsirl, Kanokwan Jaiprasert, Rungtip Nooma, Awadseda Sukgas€m, Supang Xhondee
2Q
FEASIBILITY OF ORAL IMMUNIZATION AGAINST JAPANESE ENCEPHALITIS VIRUS USING CHITOSAN
PARTICLES
Supavadee Boontha, Worawan Boonyo, Hans E. Juntinger, Tasana Pitaksuteepong,
Neti Waranuch, Assadant Polnok, Narong Nitatpattana, Sutee Yokan
251
AT ORTHO ( o ) AND PARA ( p ) POSITIONS
MET1LBENZOAT AGAINST SYNTHESIS OF HIDROKSIBENZOHIDRAZIDA DERIVATIVES
THE INFLUENCE OF HYDROXY GROUP
&.Eana, Adita
& Melanny lla
.S.
oN
Junl Etowati, Marcellino Rudyanto, Hadl Po€rwono,
Tutuk Budiati
254
ANTIOXIDANT ACTIVITY TEST OF BEE PROPOLIS EXTRACT (Apis mellitura L.) USING DPPH (1,1diphenyl-2-picrilhidrazyl) FREE RADICALS SCAVENGING ACnVIW
fitiek Martati, Shahyawidya
CAPSULE FORMUTATION
and
258
EVALUATION COMBINATION OF AQUEOUS EXTRACT OF Phaleria's
(Phaleria macrocarpa (scheff Boero) FRUITS and LEAVES as ANTIHIPERTENSIVE AGENT
IlTlSli:T-Y:Y-::::11:]:1::**:::::::::Y:::::::::l;
MANUfACTURE AND CHARACTERIZATIOI{ OF SOTIO OISPERSION GLIKTAZI} PVP K9O
,......,.,......,.. 264
H Sutama, Fikri Alatas, Clcih Aw ,{lngslh
...................
T.ita
ANTI]NFIAMMATORY ACTIVITY OF PARA METHOXY CINNAMIC AOO (PMCA) IN NANOEMUTSION
USING SOYBEAN OIL
Tristiana Erawati M, Annele lndraswarl P, anda Ghemasih .c, t{oorma Roslta, suwaldl
M
26
artodihardio, Widii Soerattl
PHYSICAT CHARACTERISNCS AND PENETRATION OF DICLOFENAC SODIUM NIOSOMAT SYSTEM
USING SPAN 20 AND SPAN 60
Tutiek Pururand, Esti Hendndl, Noverlka A. Putrl, ,{urtya r,
Dfli ......,.......,...,......,......,..,... 271
FORMULATION AND CHARACTER,SZATION OF JUICE OF LIME GEL USING CMC.NA BASE
275
Uswatun Chasanah, Estl Hendradl, lnayah
HEPATOPROTECTIVE ACTIVITY OF Bidens pilosa L. lN CARBON TETRACHLORIDE INDUCED
H
EPATOTOXICITY IN RATS
274
vina Alvionita soesilo, CJ. Soegihardjo
CYTOTOXIC ACTIVITY ASSAY AGAINTS HEIA CELL LINES oF NoVEt ANTICANCER DRUG i N(PHENYLCARBAMOYT)ISOEUTIRAM IDE
wlmzy Rlzgy Prabhata, Tri widiandani, Siswandono
........
.,...........,...... 284
SIMPLE STEPS PURIFICATION OF RECOMBINANT HUMAN ERYTHROPOIETIN PROOUCED IN CHINESE
HAMSTER OVARY CELL CULTURE
Yana Rubiyana, Endah PuJl Septisetyani, Adi
Santoso,......
.......'.'....'..... 287
KINETICS STUDY COCRYSTALS KETOCONAZOIE.SUCCINIC ACID PREPARED WITH SLURRY METHOD
BASED ON POWDER X-RAY DIFFRACTION (PXRD)
Yuli Ainun Naiih, Dwi Setyawan, Achmad Radjaram
.............
.........".'.'. 29o
CONSTRUCNON OF RECOMBINANT IMMUNOTOXIN ANti.EGFRVIII 5CFV::HPR FUSION PROTEIN AND
INDUCIBLE EXPRESSION lN Pichia pastoris AS A TARGETED DRUG cANDIDATE
Yullawatl, Asrul Muhamad Fuad
.........
Of EVOFTOXACIN BY COADMINISTRATION
zamrotul lzzah, Toetik Aryani, Amalia llllyyln, Budl Supraptl
ATTERED PHARMACOXINETIC
..----..'....'.'
297
OF ATTAPULGITE
303
j
@wffio*
Proceeding
Itelohrtenradonal
Gonfiercnce
on Pharma€euti€s & Pharmaceutical Sciences
IN VITRO ANTIMATARIAT ACTIVIW OF CHTOROFORM SUBFRACTION
OF SALAM BADAK TEAVES (Acmena acuminatissima)
Erna Cahvaninrsih. Akademi Farmasi Saraswati, Denpasat Eali; A(bn3C-flltC-lhfd. Depanment of Pharmacognosy and Phytochemistry Faculty of Pharmary, Univecitas Airlangga, Surabaya 60826, lnstitute of Tropical
Disease, Universitas Airlaogga, Surabaya 60115; AElflidaBllyeli., Department of Pharmacognosy and Phytochemistry Faculty of Pharmacy, Universttas Airlangga, Surabaya 50826, lnstitute of Tropical Disease, Universitas
Airlangga, Surabaya 50115, [email protected]
INTRODUCTION
Malaria is still an endemic disease in more than
Fractionatlon
90 countries, mainly in developing countries
the fractionation
(Sanchez et a1.,2004). Patients infected with
malaria have doubled in the last two decades.
A.acuminatissima leaves was separated by
vacum liquid chromatography (VLC) using
heune, chloroform, and ethanol at gradient
condition. This separation was produced six
subfractions, then evaporated using a vacum
evaporator and dried. These six subfractions
was then analyzed by TLC and tested for anti-
This occurred mainly due to the emergence
of resistant strains of Plasmodium falciparum
malaria to the chloroquine and its derivatives
(Trape et al.,2OO2l. Perez et al. (1997) stated
that the global spread of malaria parasites
that are resistant to current antimalarial drug
is a major health problem. Therefore, it is need
to find new antimalarial substances to replace
current drugs that are not sensitive anymore.
One attempt to discover new antimalarial substances is through active exploration of the
natural sources,
It is known that some species of the family
Myrtaceae has an antimalarial activity. Previ-
ous $udies shown that ethanol extract and
chloroform fraction
of A. acuminatissima
leaves (Myrtaceael were exhibited antimalarial activity with lC50 value of 0.040 gglml and
0.005 pg/ml, respectively. Therefore, the separation ofthe chloroform fraction was done to
determine the antimalarial activity substances
of A.acuminatissima.
MATERIALS AND METHODS
Plant materials
A.acuminatissia leaves was obtained from
Purwodadi Botanical Garden on December
2012. Sample was authenticated by the authority of Purwodadi Botanical Garden, Pasuruan, East Java.
92
Chloroform fractions which obtained from
of
ethanol extract of
malarial activity.
ln vitro antimalarial activity test
Antimalarial in vitro test was performed based
on Budimulya et al. (1997). Sample prepared
in serial dilution at concentration of 0.01; 0.1;
1; 10 and 10O Eglml in microwells. Each microwell was added with 50O El parasite culture
(1% parasitemia, 5% haematocrit) and incubated for 48 hours in 37oC. After incubation,
thin blood smears were made and stained
using 20% giemsa dye. Percentage of parasitemia was determined by countinB infected
erythrocytes per L000 total eMhrocytes under microscope.
RESULTS
AND DISCUSSION
The separation results of A.acuminatissima
leaves chloroform fraction was produced
six subfractions (A-F). Each subfraction was
tested for in vitro antimalarial activity against
P falciparum (3D7). According to Rasoanaivo
et al. (2004), an extract is very active if lC50 <
5 E/mL, active 5 prglmL < lC50 < 50 gglml,
weakly active 50 Uglml < lC50 < 100 Uglml
and inactive lC50 > 100 Ug/mL. The test re-
koceeding
ft clo hteration* Gonfrrcr
@wffio*
on Pharmaceutirs & Pharmaceutical Sciences
sults showed that all chloroform subfractions
classified as very active and subfraction E was
the most active with lC50 value of 0.007 pgl
mL. The result of antimalarial activity test of
chloroform subfractions (A-F) can be seen in
Table 1.
Figure 1. TLC chromatogram of chloroform
subfraction using silica gel F254 as stationary phase and chloroform-methanol (98;2) as
mobile phase, viewed under UV light : (a) 254
nm; (b) 366 nm; (c) after sprayed with 10%
H2SO4 and heated at 1o5oc for 5 minutes. (d)
ldentification of chloroform subfraction was
performed by TLC method using silica gel
GF254 as stationary phase and chloroform:
methanol (98:2) as a mobile phase. Then observed under UV light in wavelength of 254
nm, 366 nm and sprayed with H25O4 10%. TLC
366 nm after sprayed with 10% H2SO4 and
heated at 105oC for 5 minutes; K = chloroform
fraction, A-F = subfraction.
chromatogram profile showed that allsubfractions contain purple spot (Fig.1). According to
sharifa et al. 120!21, terpenoid compounds
will form a pink to purple or violet after being
sprayed with lV/o HZSO4.lt can be considered
that all subfractions were containing terpe-
Subfraction A-F were exhibited antimalarial
activity (1c50 value of 0.007-1.469 Bglml) and
containing terpenoids substances. Based on
that result, it is possible to conclude that antimalarial activity of subfractions was derived
from terpenoids substances.
i---1
= considered to contain terpenoids.
noids.
coNctustoN
lc,
Sub
ogr1
(rahl)
lm
l0
I
r)
0l
0.01
100
100
15.32
6J,III
56.19
0.0t2
B
1fi,
81.39
63.95
47 69
24.32
0.158
C
86.41
83 94
41.38
l5.l?
20.40
I -469
D
9690
92.48
64.81
15.5
26.40
o.t7z
E
loo
E5
?0
''t0.42
69_E4
56.75
0.m1
F
97.19
er.1r 68rr
59 8t
54.61
0.0t4
I
Table 1. lC50 values of Chloroform subfractions against P falciparum 307
Iti
i\\ltcl)|l
c
h
Subfraction E of A.acuminatissima Ieaves was
the most activ€ as antimalarialwith lC50value
of 0.007 uglml. The antimalarial activity was
possible derived from terpenoids substances.
Further study needed to isolate and identify
the active compounds from A.acuminatissima
ACKNOWLEDGEMENT
The authors acknowledge Satreps Lab-Natural
Product Medicine Research Development Division, lnstitute of Tropical Dlseases Universitas Airlangga for suporting this research.
RIFERENCES
1.
2.
l:-------.--t --'---
K-\
I
BCD
d'
Budimulya, AS., Syafruddin, Tapchaisri,
P, et al. 1997. The sensitivity of Plas
modium protein synthesis to prokary
oticribosomal inhibitors. Mol Biochem
Parasitol 84:137-141
Perez, H., Diaz, F., Medina, jD.,
1997. Chemical investiSabon and in vitro
antimalarial activity of Taebuina ochra
cea ssp. neochrysantha. lnternational
3.
Journal of Pharmacognosy 35(4) : 22723L.
Rasoanaivo, P., Oehar, OE., Ratsimaman
93
@
to+ffiou
ga, U., et al. 2004. Guidelines for The
Nonclinical Evaluation
The Efficacy
of Traditional Antimalarials. ln : Tradi
tional Medicinal Plants and Antimalaria.
CRC Press. USA 256-258.
Sanchez, BAM., Mota, MM., Sultan, AA.,
et al. 2004. Plasmodium berghei parasite
transformed with green fluorescent pro
tein for screening blood schizontocidal
agents. lnt. J. of Parasitology 34 : 485490.
of
4
94
Proceeding
net-nen*oatGorirrgp
on Pharmaceutics & Pharmaceutical Sciences
5.
6.
Sharifa, AA., Jamaludin, J., Kiong, 15.,
et al. 2012. Anti-Urolithiatic Terpenoid
Compound from Plantago major Linn.
(Ekor Aniing). Sains Malaysiana, Vol.
41(1):33-39.
Trape. JF-, Pison, G., Speigel, A., et al.
2002. Combating malaria in Africa. Trends
in Parasitology 18 :224-230.
f
..___*t_
Organized
by:
FACULWOF PHARMACT
UNTVEFSITAS AIRLANGGA SU RABAYA
www.i
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Proceeding
Thelohternadonal
I
T
on Pharmaceutics & Pharmaceutical Sciences
Drug Delivery Systems:
From Drug-Discovery, Pre-formulation, Formulation and
Poorly Soluble Drugs and Protein
-1a-fl
:
--.-
Proceeding
*
fl re I lnternational Gorfercme
on Pharmaceutics & Pharmaceutical Sciences
Published and Organized by
Faculty of Pharmacy
Universitas Airlangga
Surabayalndonesla
2014
h-a
The l"t I nternationa I Conference on Pharmaceutics
& Pharmaceutical Sciences Proceedings
ISBN : 97&602-72333-G.o
(Lefter of Natlonal ISBN Agency No. 4127E.8/p/03.2015 Date 18 March 2015)
lst edltion Proceedlng
Publlshed by:
Faculty of Pharmacy UniveBitas Airlangga
Surabaya, lndonesia
Addr€ss:
Kampus B rl. Dharmawangsa Dalam
Surabaya 60286
Phone +52 31 5033710
Fax +62 31 5020514
Website: www.icpps2ol4.com or www.ff.
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PREFACE
From Chairman
It is our
pleasure to present you the proceedings of The 1st lntemational Conference on
Pharmaceutics and Pharmaceutical Sciences (ICPPS) oBanized by The Faculty of Pharmacy
Universitas Airlangga Surabaya lndonesia.
The proceeding was produced based on papers and posters presented at The 1st lntemational
Confurence on Pharmaceutics and Pharmaceutical Sciences (ICPPS), held in Sunbaya, lndonesia, 1415 November2014.
The proceeding clearly reflects broad interest, from the participants thatcoming fmm allaround the
world.
The papers presented were pharmaceutics and biopharmaceutics; requirements on how to e\raluate
molecules in discovery and their appropriateness for selection as potential candidate; their
development in context of challenges and benefits, together with associated time and cost
implications and also requirements to progressthrough pre{linieland clinical.
ln this an opportunity, I would like to express my appreciation to the editorialteam ofthe proceeding
who hrve been workin8 hard to review manuscripts, and makingthe first edition ofthis proceedin8
be possible.
I would like also to thanks to all invited speakers and presenters who participated in The lst
lnternational Conference on Pharmaceutics and Pharmaceutical Sciences (ICPPS) and your
mntribution to this proceeding.
Finally,
I hope this proceeding will give contribution to the Pharmaceutics and Pharmaceutical
Sciences research.
Chairman,
Dra. Esti Hendradi, MSl,, Ph.D., Apt
COMMITTEE
Advisor
Chairman
: Dean of Faculty of Pharmary Universitas Airlangga
: Dra. Esti Hendradi, M.Si., Ph.D, Apt.
Editor
: Dr.
Wouter U. Hinrichs (Netherlands)
Prof. Kozo Takayama (Japan)
Mutalik (lndia)
Dr. Nuttakorn Baisaeng (Thailand)
Prof. Dr. Widji Soeratri, DEA (lndonesia)
Dr. Srinivas
Committee
Prof. Dr. Widji Soeratri, DEA, Apt.
Prof. Dr. Siswandono, MS., Apt.
Prof .Dr. Tutuk Budiati, MS., Apt.
Prof. Dr. rer.nat. Muhammad Yuwono, MS., Apt,
Drs. Hadi Poerwonq MSc.,Ph.D, Apt.
Drs. Marcellino Rudyanto, MSi., Ph.D, Apt.
Drs. Bambang Widjaja, M.Si, Apt.
Drs. Sugiyartonq MS, Apt.
Dra.Tutiek Purwanti, MSi, Apt.
Dra.Tristiana Erawati, MSi, Apt.
Dra.Retno Sari, MSc, Apt.
Dra.Dewi lsadiartuti, MSi, Apt.
Dra.Noorma Rosita, MSi, Apt.
Dr. Dwi Setyawan, SSi, MSi., Apt.
Dr. Riesta Primaharinastiti, SSi, MSi, Apt.
Dewi Melani Hariyadi, SSi, M.Phil., Ph.D, Apt.
Helmy Yusuf, SSi, MSc., Ph.D, Apt.
Dr. rer. nat. Maria Lucia Ardhani Dwi Lestari, SSi,MPharmSci., Apt.
Melanny lka Sulistyowati, SFarm, M.Sc, Apt.
Febri Annuryanti, SFarm,M.Sc, Apt.
M. Faris Adrianto, MFarm, Apt.
Dini Retnowati, SFarm,Apt.
Abhimata B SFarm, Apt.
of CONTENT
TABI,T
Preface from Chairman
Committee
tl
Table of Contents
iii
Author lndex
iii
AUTHOR INDEX
COMPARISON OF SODIUM STARCH GLYCOLATE AND CROSSCARMELTOSE SOOIUM AS
SUPERDISINTEGRANT IN MEFENAMIC ACID FAST DISINTEGRATING TABI.ET
Adeltrudls Adelsa D, Oktavia Eka Pusplta, Amalla Ayuningtyas, Marulita lsadora
1
STUDY EXPRESSION OF HUMAN ERYTHROPOIETIN EXPRESSSION IN MAMMAUAN CELL
Adl samoso, Popl Hadiwisnuwardhani, Yana Rublana, Yulalka Romadhani, Endah Puji
Septisetyanl, Dyanin$yas D,P, Putri
4
ANTIOXIDANT STABILITY ASSAY OF ATPHA TOCOPHERYI- ACETATE IN SOLID LIPID NANOPARTICLE
SYSTEM
AnSEie
(t,PlD eqSE BEESWAX AND MONOSTEABIC 6LISEBYL 50:50)
wldhi, Noorma Rosita, lMdii Soe6tri
..............,.....
A BIOACTIVE SOYINE HYDROXYAPATITrcETATIN
..,............,...,.,... 8
IMPLANT FOR IN VITRO GENTAMICIN RELEASE
13
Anlek s€dya Budiatn, M. zainuddin, Junaidi Xhotib, Diah Himawati
EFFECT OF COMPARISON SURFA TA T AND @SURFA TANT IN WATER/OII, MICROEMUTSION IN
REEASE OF OVALBUMIN Microemulsion Water/Oil with Surfactant (Span SGTween E0) :
t'"
,'t,
.nO
Cosu rfactant (Ethanol) =S'1
Anisa Rizki Amalla, Riesta Primahadnasbti, Esti Hendradi
ANALYSIS OF
MYcotlc
ACIDS CTEAVAGE PRODUCT OF
18
MycoDocterium tuberculosis BY GAS
CHROMATOGRAPHY-FIAME IONIZATION DETECTOR
Asrl Darmawati, Deby Kusumaningrum, lsnaeni, Muhamad Zainuddin
2l
PERIPI"ASMIC EXPRESSION OF GENE ENCODING ANT!EGFRvlll SINGLE-CHAIN VARIABLE FRAGMENT
ANTIBODY USING PelB TEADER SEQUENCETN ES6HER|CHIA cOLl
Kartika Sari Dewl, Debble Sofie Retnoringrum, Catur Riani, Asrul Muhamad Fuad
24
IN VIVO ANTIMALARIAL ACTIVIry OF ETHANOL EXTRACT AND ETHYT ACETATE FRACTION OF
Aledryon serrotus LEAVES ON Prasmodrum berghei INFECTED MICE
AW Widyawaruyand, Uswatun Khasanah, Lldya Tumewu, Hilkatul llmi, Achmad Fuad Haffd,
...........'.............. :lO
lndah 5 Tantular ...
PROFILE OF COMMUNITY PHARMACISTS KNOWLEDGE IN PATIENT ASSESSMENT WITH INFIUENZA
SYMPTOMS AND ITS PRODUCTS
Azza Faturrohmah, Atle Sullstyetlni, Ana Yuda
33
soLUBtLtTy AND DTSSOLUTTON SrUDY OF KETOPROFEN
lNcLUslON COMPLEX (Prepared by Kneading Method)
Bambant Wdrala, Achmad RadJaram, Arafah Zulhana
-
HIDROXYPROPYL-p-CYCLODETTRIN
,,.....
,..........'.......... 37
FORMUTATION AND STABILITY TESTING OF MELOXICAM SOLID DISPERSION GEL
Budlpntlwl Wlsudyantnlsih, lnka Dewl Nur Angraraini, Fersiya Wardani
EFFECT OF MENTHOL AS PENEIRATION ENHANCER TO DICLOFENAC
40
SODIUM MEMBRANE-ryPED
TRANSDERMAT PATCH CHARACIERIZANON
Destrla lndah Sarl, Esd Hendradl, Junaldl Khodb
43
PHYSICAL CHARACTERISTICS AND RELEASE STUDY OF OVATBUMIN FROM ALGINATE MICROSPHERES
PREPARED BY DIFFERENT CONCENTRATION OF ALGINAIT AND 8aCI, USING AEROSOLIZATION
TECHNIQUE
Dewl Melanl liarlyadl, Trlsdana
El"yrti, sisilia Ermavahyuningtyas
45
MUCOADHESIVE TABLET OF ETHANOLIC EXTRACT OF SAMBILoTo (Andrographis paniculata)
AS ANTIDIABETIC USING CHITOSAN
Dhadhang wahyu Xumlawan, Henlng Pratiwi, and Uryga lkaditya
50
PHYSICAT INTERACTION STUDY OF IBUPROFEN.STEARIC ACID BINARY MIXTURE
Olalent Putrl Paramlta, Dwl Setyauran, Dewi lsadiartuti
....
.,.,..............,.... 59
MoLEcUtAR MoDELING AND SYNTHESIS oF 1-(3,+Dichlorobenzoyl)-1,3-dimethylurea
DIan Agun8 Pangarlbowo, Slswandono, BambangTri Purwanto
63
EXPRESSION OF RECOMBINANT HUMAN GRANULOCYTE{OLONY STIMUTATING FACTOR WITHIN
PERIPLASMIC COMPARTMENT OF Esc,}ench,o
coli USING PeIB LEADER PEPTIDE
Dlrn Fitrh Atxr3tvantl, Aerul Muhamad Fuad ,--**----.-
..,,.,,..,...,..,,....*
EVALUATION OF ANTIHYPERURICEMIC ACTIVITY FROM BULBS OF BAWANG TIWAI lEleutherine
BY lN VITRO ANO lN VIVO STUDIES
polmifolld (Ll Merr.,
Dlan Radh laksmltawaq Rlnlnta Firdaus, \tulinda, Mediana Asdka
72
lFrogorio x ononosso Duch.) AND SfARFRUff {Averrhod carombolo L.} USING ABTS
SCAVENGING METHOD
Dhna Sedahwaty, lndira Natalia
Timang
FREE
RADICAI
..,.....,...,...,.,.. 76
ENHANCEMENT OF SOLUBILrIY AND DISSOLUTION ATORVASTATIN BY MICROCRYSTATLIZATION
METHOO
Dollh Gorall, Yoga Windu wa.dhana, Ronny Tandela
79
lrv yrIRO ANTIMAIARIAL ACrIVITY OF DICHLOROMETHANE SUB-FRACTION OF Eucalyptus globulus
L, Stem AGAINST P,osmodium folcipdrum
Ells Suwarnl, Achmad Fuad Hafid, Aty WidyawarutBnti
86
Arcongelisid llavo INCREASES RATS' LEUKOCYTES BUT HAS BIPHASIC EFFECT ON RATS' LYMPHOCYTE
Endah Puspitasari, Evi Umayah Ulfa, Vlta Arlatl, Mohammad SuhhonHabibi
E9
IN V]TRO ANTIMAIARIAL ACTIVITY OF CHLOROFORM SUBFRACTION OF sALAM BADAK LEAVES
lAcme na ocu m i noti ssi mol
Erna Caf,vanlwslh,
CHARACTERIZATION
A&Drd Fud Htfd, Aly l,yidyawaruyanf ....,.,........................................92
OF DOSAGE FORM AND PENETRATION DICLOFENAC SODIUM WITH
MICROEMULSION SYSTEM lN HPMC 4000 GEL BASE (Micmemulsion
Span 80 - Tween 80 : Cosurfactan Ethanol 96% = 5:1)
WO with rasio
use of surfactant
Estl Hendndl, Tudek Purwar*i, lGrina Wahyu lrawad
95
CONSTRUCTION AND VALIDATION OF THE STRUCTURE.BASED VIRTUAL SCREENING PROTOCOts
WITH PDB CODE OF 3LN1 TO DISCOVER CYCLOOXYGENASE.2 INHIBITORS
Mumpunl
E,
Nurrcchmad A, Pranowo HD, renie UA lstyastono EP
99
VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF ASPIRIN IN RABBIT
PIAsMA : APLICATION TO BIOAVAILABIIITY STUDY OF ASPIRIN MICROCAPSULE lN RASSIT
toz
Falzatun, Novl yantih, Teguh lman Saputra
EFFECT OF COMPARISON OF SURFACTANT AND COSURFACTANT WO MICROEMULSION OVALBUMIN
WITH SOYBEAN OILTO PHYSICOCHEMICAL CHARACfERIZATION (w/o Microemulsion with Surfactant
Span 8G Tween 80 : Cosurfactant Ethanol96% = 5:1; 6:1 and 7:1)
Farlda Mutlara Sarl, Rlesta Prlmaharlnasttd, Esd Hendradi
105
pH INFLUENCE IN DESATTING PROCESS OF CRUDE PERTUSSIS TOXIN (PT) AND FILAMENTOUS
HEMAGGLUTININ (FHA) PURIFICATION FROM Bordetello pertussis BY SEPHADEX G-25 COLUMN
CHROMATOGRAPHY
Faris Adrlanto, Esd Hendradl,
tlenl ,{uElny, lsnaeni
108
SEPARATION OF COSMETIC PRESERVATIVES USING SILICA-BASED MONOLITHIC COTUMN
Febri Annuryanti, Riesta Primaharinastiti, Moch. \tuwono
111
PREPARATION AND CHARACTERIZATION OF TELMISARTAN.CITRIC ACID CO.CRYSTAL
Fikri Alatas, Hesdary Ratih,
Sundani umno So€wandhie
114
PATIENTS' AND CAREGIVERS' LIQUID MEDICATION ADMINISTRATION ERRORS
Gusti Noorrizka vemnika Achma4 Gesnita Nugrahenl
.,.,
,...,.......,......,.... 117
THE POTENCY OF CANARIUM OIL (Conarium indicuml AS A MATERIAL FOR STRUCTURED LIPID
PRODUCTION
Hamidah Rahman, Johnner
P
Sitompul, lfusnandar Angtadiredja, Tutus Gusdlnar .,........,.,.. 121
EFFECT OF TREHALOSE ON THERMAL PROPERTIES OF PHOSPHOTIPID-DDA AND TPGS MIXTURES
Helmy Yusuf
.......,.
,.,...,..,.....,..,,.,,, 124
PEEPABATION AND CHABACTEB'ZATION OF FLUKONAZOLE.9.CYCLODEXTRIN INCLUSION COMPLEXES
Hestiary Ratlh, Flkrl Alatas, Erin xarlina
L27
ISOLATION AND IDENTIFICATION OF ANIIOXIDAI{T COMPOUND BY BIOPRODUCTION OF
ENDOPHYTIC FUNGI OF TURMERIC (Curcuma longo L.l ISOLATE CL.SMl.RF11
Hindra Rahmawati, Bustanussalam, Partomuan Simaniuntak
130
MODIFICATION PROCESS OF NATURAT CASSAVA STARCH : THE STUDY OF CHARACTERISTICS AND
PHYSICAT PROPERTIES
Prasetia, Jemmy A, C,l.S, Arlsanti, I{.P.P,A. Dewl,
6-A.i. Astutf, l{,W,il Yulianiqsih, I M.A.G.
Wirasuta
133
ORUG USE PROFILE OF DIABETIC PATIENTS IN EAST SURABAYA PRIMARY HEALTH CARE
I ilyoman
wijata, A:ra Fatunohmah, Ana Yuda, Mufartlha,
Hlkmah Prasasti
t'1,
Tesa Geoyanl santoso, Dlna xartlka,
Whanni Wido Agustin
136
GLYCINE MAX DETAM II VARIEry AS PREVET{TIVE AND CURATIVE ORGAN DAMAGE DUE TO
EXPOSURE TO ,LEAD (Pb)
Rlka Yulla, Sylvan Septian R6sandy, Gusd AW Putu Puspikaryani, I Putu Atus Yulyastr"wan, D€wa
lq2
AW Kusuma Dewi
AN ACTIVITY TEST OF MATOA LEAVES EXTRACT AS HEART RATE FREQUENCY REDUCTION WITH
ADRENATINE INDUCTION
lk
t4
Purwldy.nlntrum, Elln Yulinah Sukandar, lda Fidrianny
EFFORT TO REDUCE COMPRESSIBITITY OF RAMIPRILTHROUGH CRYSTAT ENGINEERING
lnd6, Smdanl
N
So€sendhi
..-.,,.,...*..*
1|7
IN VITRO ALPHA-GLUCOSIDASE INHIBITORY ACNVIY OF ETHANOLIC LEAF EXTRACT AND FRACNONS
Rouvofio seryenliino (1.) Benth. ex Kurz
Julle Anne D. Bolaftos, lvan L Lawag
OF
.,
.........,............ 15o
PERIPTAsMIC EXPRESSIOI{ OF GENE ENCODING AI{TFEGFRVIII SIN6I.E{HAIN VARIABI.E FRAGMENT
ANTIEO0Y USING PelB LEADER SEQUENCEIN Escherichio coli
(artika Sari Oewi, Debbie Sofie Retnoningrum,catur Riani, Asrul Muhamad Fuad ...,.,,....,. 153
CHARACTERIZATION
AND LD- VALUE DETERMINATION OF
1,s-bis(3'-ethoxy{'-hydroxyphenyl}-1,4-pentadiene.3-one
testarl Rahayu, Septian, Esti
(EHP)
Mumpuni,
,..,..,.,.,........ 159
DEVELOPMENT OF MELOXICAM TRANSDERMAT MATRIX TYPE PATCH USING
POLWINYTPYRROI-IDONE, HYOROXYPROPYL METHYTCELTULOSE, AND ETHYI- CELI.ULOSE
COMBINATION
t62
tldya Am.llana, Monica lwud, Selly Rlo
ANTIHEPATITIS C VIRUS ACTIVITY SCREENIN6 ON
Harpul,io orboreo EXTRACTS AND ISOLATED
COMPOUND
Lldya Tumewu, Evhy Apryani, Mei Ria Sant, Tutik Srl Wahyuni, Adita Ayu Permanasa4
Myma Adlantl, Chle Aoki, Aty Widyawarufanti, Adrmad Fuad Hafd, Maria lnge Lusida,
......,.....,....... 165
Hotta
Soetjipto, Hak
HPLc METHoD PRECISION TO ASSAY OF A-MANGOSTIN lN Mangosteen (6orc,nia mongostano L.l
FRUIT RIND EXTRACT FORMULATED IN ORAL SOLUTION
Liliek Nurhidafat, Siti Sofiah, Ros Sumarny, Xevin
Caesar.
....,......,,....... 168
;qiPARATION AND CHARACTERIZATION OF NARINGENIN.LOADED CHITOSAN NANOPARTICLES FOR
OiEMOPREVENTION
Lira lMnarti, Lusla Oktora Ruma Kumala Sari
t70
IE'.ATIONSHIP OF KNOWLEDGE AND PATIENT BEHAVIOR ON SELF MEDICATION PIROXICAM
i Sludies of Pharmacy in Sukun District, Malang City)
Ljza Pristianty, Reshtia Erlana Putd, Hldayah Rachmawatl
t7t
iFFECT OF CHRONIC USE OF ENEBGY DBI'IX ON XIDNEY
tlahardlan Rahmadi, Zamtotul lzzah, Mareta Rindant A, Aniek Setya
SCREENING OF SURFACE MODIFIERS TO PRODUCE
GUIDANCE
Mada Lucia Ardhani
B,
Suhadono ........... 176
SIABE NANOSUSPENSION : A GENERAI
llxi l€stari ..,......
............,......... 179
,EVETOPMENT OF SIMPLE POLYPHENOL SENSOR BASED ON SODIUM META PERIODATE AND 3.
METHYT-2.BENZOTHIAZOLINONE HYDRAZONE FOR COFFEE SAMPLES
t
och. Amrun
Hidayat
indya Pusp'rtaningtyas, Agus Abdul Ganl, Bambant Kuswandi ...,.. 181
VALIDATION OF AN HPLC ANALYTICAL METHOD FOR DETERMINATION OF LEVOFLOXACIN IN
OPHTHALM IC PREPARATIONS
Mochamad Yuwono, Riesla Primaharlnastiti, Ateng Teguh Wbrdoyo
VAUDATION OF SPECTROPHOTOMETRIC
IN TABLET DOSAGE FORM
Nia Xrlsfnlntrum, Diah Yuli ParyEsti
184
M€THOD FOR ESTIMATION OF EPERISONE HYDROCHLORIDE
...
..,...,.......,..... 187
ANNOSTEOPOROTIC ACNV|TY OF 96% ETHANOUC EXTRACTS OF ABELMOSCHUS MANIHOT LMEDIK
LEAVES AND EXERCISE ON INCREASING BONE DENSITY OF FEMALE MICES FEMORALTRABECULAR
190
Niliestria Ayu Faramitha Sholikhah
EFFECT OF {YCTODETTRIN ON sPF VALUE AND INHIBITION OF KO.,IC ACIDSTYROSINASE ACTIVTTY
IN VANIsHING CREAM BASE FORMUTATION (ON SUNSCREEN PRODUCT CONTAINED OXYBENZONE)
Noorma Rosita, Diana, Diana winarlta, Tristiana Erawati, Wldji sroeratrl
193
ANTIMICROBIAL ACTIVITY OF LACTOBACILLI PROBIOTIC MILK AND GUAVA I FAF ETHANOLIC EXTRACT
(Psidium guajava) COMBINATION AGAINST BACTERIAI CAUSE OF DIARRHEA
197
ur Putri Ranti,lsnaeni, Juniar Moechtar, Febri Annunranti
THE INTTUENCES OF PARTICTE SIZE AND SHAPE ON ZEIA POTENTIAL OF COENZYME Q1O
NANOSUSPENSION
Nuttakom
Baisaeng
.......'.'.'....'... 200
SYNTHESIS, MOIECUTAR DOCKING, AND ANTITUMOS ACTIVITY OF
Dimethylurea AGAINST HUMAN BREAST CANCER CELL LINE (MCF-7)'\I,NLDibENZOYI-N,NL
..".'......'........ 203
Nuzul walryuning Diyah
.........,..,....,....
EXPRESSION OF ANII-EGFRVIII SINGLE CHAIN FRAGMENT ANTIBODY (SCFV)ON THE SURFACE OF
PICHIA PASTORIS
hlka vio&rfi,
Asrut Muh.m.d Fuad,
tuftarsono
.'...'..'.'.---.'.
z)5
SUBCLONING OF csf3syn (COLONY SnMULATING FACTOR-3) GENE INTO pGAPZ? AND
TRANSFORMATION OF RECOMBINANT VECTOR INTO PICHIA PASTORIS
Prety lhda Arfia, Asrul Muhamad
Fuad
,...............,...212
THE USE OF PERICARP MANGOSTEEN (Garcifiia mangostana
CREAM-TYPE O
L) EXIRACr lN
FORMUTATION OF
/W
Rahmah Elfiyanl, ilaniek setladi Radjab, Mla Sadta soffan ,.,.,.....,.,...,,.........,...,...,,..,...,... 218
CHITOSAN BASED PARTICULATE CARRIER OF DITIRPENE LACTON OF SAMBILOTO PREPARED BY IONIC
GELATION-SPRAY DRYING :EFFECT oF STIRRING RATE AND NOZI..E DIAMETER
Retno Sad l-run Suhartanti, Ihxt Setyawan, Esd Hendrrdl, WUI Soeratrt.-.--
*--.*---.....2X2
GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHOD VALIDATION FOR PESTIODES RESIDUES
ANALYSIS IN FOOD USING QUEChERS KIT
22s
Rlesta Prlmaharinastid, Setyo Prlhatinlngty'.s, Mochammad Yuwono
CHARACIERIZATION OF PARACETAMOL ORAUY DISINTEGRATING TABLET USING GEI.ATIN 1% ANO 2%
AS BINDER AND POLYPTASDONE XL-1O 10% AS DISINIEGRANT (Prepared by Freeze Drying Method)
Roisah Nawadla, D{ri Setyawan. Bambant wldjaja
230
ANTIox|DANT STUDY oF COSOTVENT SOLUTION OF MANGOSrEEN (Garcinia manBostana L) RIND
EXTRACT IN RATS BY USING MDA PARAMETER
Ros Sumamy, Liliek ,{urhldafad, Yat Sumlyati, Fransiska Dlana Sanii...........,......,.,...,.,.,.,. 233
SCREENING OF SELECTED PHILIPPINE ROOT CROPS FOR ?-Glucosidase INHIBITION
SaEh Jane
S.
Almazora, lrran L Lawat
...
............,...... 236
NIOSOME EMULGEL FORMUTATION AND STABLITY TEST OF CINNAMON (Gnnamomum burmanii
Nees & Th. Nees) Eark ETHANOIIC EXfRACf AS ANTIoXIDANT
Sasand Dariianto, Fldrhni |rda, Widhita
Pff
239
THEOPHYLTINE RELEASE FROM SUSTAINED RELEASE TABLET USING LACTOSA AND PVP K3O A5 A
CHANETIING AGENT
Sugiyartono, Retno sari, Agus Syamsur Rlral, TriMuhanl, Atustin Maharani
242
EFFORTS TO PRODUCE 1-(EENZOYLOXY)UREA
AS ANTICANCER DRUG CANDIDATE
suko Hardjono
245
DEVELOPMENT OF PEGYIAtEd RAPAMYCIN LITHOCHOTIC ACID MICELLE FOR CANCER THERAPY
Ann Tapsirl, Kanokwan Jaiprasert, Rungtip Nooma, Awadseda Sukgas€m, Supang Xhondee
2Q
FEASIBILITY OF ORAL IMMUNIZATION AGAINST JAPANESE ENCEPHALITIS VIRUS USING CHITOSAN
PARTICLES
Supavadee Boontha, Worawan Boonyo, Hans E. Juntinger, Tasana Pitaksuteepong,
Neti Waranuch, Assadant Polnok, Narong Nitatpattana, Sutee Yokan
251
AT ORTHO ( o ) AND PARA ( p ) POSITIONS
MET1LBENZOAT AGAINST SYNTHESIS OF HIDROKSIBENZOHIDRAZIDA DERIVATIVES
THE INFLUENCE OF HYDROXY GROUP
&.Eana, Adita
& Melanny lla
.S.
oN
Junl Etowati, Marcellino Rudyanto, Hadl Po€rwono,
Tutuk Budiati
254
ANTIOXIDANT ACTIVITY TEST OF BEE PROPOLIS EXTRACT (Apis mellitura L.) USING DPPH (1,1diphenyl-2-picrilhidrazyl) FREE RADICALS SCAVENGING ACnVIW
fitiek Martati, Shahyawidya
CAPSULE FORMUTATION
and
258
EVALUATION COMBINATION OF AQUEOUS EXTRACT OF Phaleria's
(Phaleria macrocarpa (scheff Boero) FRUITS and LEAVES as ANTIHIPERTENSIVE AGENT
IlTlSli:T-Y:Y-::::11:]:1::**:::::::::Y:::::::::l;
MANUfACTURE AND CHARACTERIZATIOI{ OF SOTIO OISPERSION GLIKTAZI} PVP K9O
,......,.,......,.. 264
H Sutama, Fikri Alatas, Clcih Aw ,{lngslh
...................
T.ita
ANTI]NFIAMMATORY ACTIVITY OF PARA METHOXY CINNAMIC AOO (PMCA) IN NANOEMUTSION
USING SOYBEAN OIL
Tristiana Erawati M, Annele lndraswarl P, anda Ghemasih .c, t{oorma Roslta, suwaldl
M
26
artodihardio, Widii Soerattl
PHYSICAT CHARACTERISNCS AND PENETRATION OF DICLOFENAC SODIUM NIOSOMAT SYSTEM
USING SPAN 20 AND SPAN 60
Tutiek Pururand, Esti Hendndl, Noverlka A. Putrl, ,{urtya r,
Dfli ......,.......,...,......,......,..,... 271
FORMULATION AND CHARACTER,SZATION OF JUICE OF LIME GEL USING CMC.NA BASE
275
Uswatun Chasanah, Estl Hendradl, lnayah
HEPATOPROTECTIVE ACTIVITY OF Bidens pilosa L. lN CARBON TETRACHLORIDE INDUCED
H
EPATOTOXICITY IN RATS
274
vina Alvionita soesilo, CJ. Soegihardjo
CYTOTOXIC ACTIVITY ASSAY AGAINTS HEIA CELL LINES oF NoVEt ANTICANCER DRUG i N(PHENYLCARBAMOYT)ISOEUTIRAM IDE
wlmzy Rlzgy Prabhata, Tri widiandani, Siswandono
........
.,...........,...... 284
SIMPLE STEPS PURIFICATION OF RECOMBINANT HUMAN ERYTHROPOIETIN PROOUCED IN CHINESE
HAMSTER OVARY CELL CULTURE
Yana Rubiyana, Endah PuJl Septisetyani, Adi
Santoso,......
.......'.'....'..... 287
KINETICS STUDY COCRYSTALS KETOCONAZOIE.SUCCINIC ACID PREPARED WITH SLURRY METHOD
BASED ON POWDER X-RAY DIFFRACTION (PXRD)
Yuli Ainun Naiih, Dwi Setyawan, Achmad Radjaram
.............
.........".'.'. 29o
CONSTRUCNON OF RECOMBINANT IMMUNOTOXIN ANti.EGFRVIII 5CFV::HPR FUSION PROTEIN AND
INDUCIBLE EXPRESSION lN Pichia pastoris AS A TARGETED DRUG cANDIDATE
Yullawatl, Asrul Muhamad Fuad
.........
Of EVOFTOXACIN BY COADMINISTRATION
zamrotul lzzah, Toetik Aryani, Amalia llllyyln, Budl Supraptl
ATTERED PHARMACOXINETIC
..----..'....'.'
297
OF ATTAPULGITE
303
j
@wffio*
Proceeding
Itelohrtenradonal
Gonfiercnce
on Pharma€euti€s & Pharmaceutical Sciences
IN VITRO ANTIMATARIAT ACTIVIW OF CHTOROFORM SUBFRACTION
OF SALAM BADAK TEAVES (Acmena acuminatissima)
Erna Cahvaninrsih. Akademi Farmasi Saraswati, Denpasat Eali; A(bn3C-flltC-lhfd. Depanment of Pharmacognosy and Phytochemistry Faculty of Pharmary, Univecitas Airlangga, Surabaya 60826, lnstitute of Tropical
Disease, Universitas Airlaogga, Surabaya 60115; AElflidaBllyeli., Department of Pharmacognosy and Phytochemistry Faculty of Pharmacy, Universttas Airlangga, Surabaya 50826, lnstitute of Tropical Disease, Universitas
Airlangga, Surabaya 50115, [email protected]
INTRODUCTION
Malaria is still an endemic disease in more than
Fractionatlon
90 countries, mainly in developing countries
the fractionation
(Sanchez et a1.,2004). Patients infected with
malaria have doubled in the last two decades.
A.acuminatissima leaves was separated by
vacum liquid chromatography (VLC) using
heune, chloroform, and ethanol at gradient
condition. This separation was produced six
subfractions, then evaporated using a vacum
evaporator and dried. These six subfractions
was then analyzed by TLC and tested for anti-
This occurred mainly due to the emergence
of resistant strains of Plasmodium falciparum
malaria to the chloroquine and its derivatives
(Trape et al.,2OO2l. Perez et al. (1997) stated
that the global spread of malaria parasites
that are resistant to current antimalarial drug
is a major health problem. Therefore, it is need
to find new antimalarial substances to replace
current drugs that are not sensitive anymore.
One attempt to discover new antimalarial substances is through active exploration of the
natural sources,
It is known that some species of the family
Myrtaceae has an antimalarial activity. Previ-
ous $udies shown that ethanol extract and
chloroform fraction
of A. acuminatissima
leaves (Myrtaceael were exhibited antimalarial activity with lC50 value of 0.040 gglml and
0.005 pg/ml, respectively. Therefore, the separation ofthe chloroform fraction was done to
determine the antimalarial activity substances
of A.acuminatissima.
MATERIALS AND METHODS
Plant materials
A.acuminatissia leaves was obtained from
Purwodadi Botanical Garden on December
2012. Sample was authenticated by the authority of Purwodadi Botanical Garden, Pasuruan, East Java.
92
Chloroform fractions which obtained from
of
ethanol extract of
malarial activity.
ln vitro antimalarial activity test
Antimalarial in vitro test was performed based
on Budimulya et al. (1997). Sample prepared
in serial dilution at concentration of 0.01; 0.1;
1; 10 and 10O Eglml in microwells. Each microwell was added with 50O El parasite culture
(1% parasitemia, 5% haematocrit) and incubated for 48 hours in 37oC. After incubation,
thin blood smears were made and stained
using 20% giemsa dye. Percentage of parasitemia was determined by countinB infected
erythrocytes per L000 total eMhrocytes under microscope.
RESULTS
AND DISCUSSION
The separation results of A.acuminatissima
leaves chloroform fraction was produced
six subfractions (A-F). Each subfraction was
tested for in vitro antimalarial activity against
P falciparum (3D7). According to Rasoanaivo
et al. (2004), an extract is very active if lC50 <
5 E/mL, active 5 prglmL < lC50 < 50 gglml,
weakly active 50 Uglml < lC50 < 100 Uglml
and inactive lC50 > 100 Ug/mL. The test re-
koceeding
ft clo hteration* Gonfrrcr
@wffio*
on Pharmaceutirs & Pharmaceutical Sciences
sults showed that all chloroform subfractions
classified as very active and subfraction E was
the most active with lC50 value of 0.007 pgl
mL. The result of antimalarial activity test of
chloroform subfractions (A-F) can be seen in
Table 1.
Figure 1. TLC chromatogram of chloroform
subfraction using silica gel F254 as stationary phase and chloroform-methanol (98;2) as
mobile phase, viewed under UV light : (a) 254
nm; (b) 366 nm; (c) after sprayed with 10%
H2SO4 and heated at 1o5oc for 5 minutes. (d)
ldentification of chloroform subfraction was
performed by TLC method using silica gel
GF254 as stationary phase and chloroform:
methanol (98:2) as a mobile phase. Then observed under UV light in wavelength of 254
nm, 366 nm and sprayed with H25O4 10%. TLC
366 nm after sprayed with 10% H2SO4 and
heated at 105oC for 5 minutes; K = chloroform
fraction, A-F = subfraction.
chromatogram profile showed that allsubfractions contain purple spot (Fig.1). According to
sharifa et al. 120!21, terpenoid compounds
will form a pink to purple or violet after being
sprayed with lV/o HZSO4.lt can be considered
that all subfractions were containing terpe-
Subfraction A-F were exhibited antimalarial
activity (1c50 value of 0.007-1.469 Bglml) and
containing terpenoids substances. Based on
that result, it is possible to conclude that antimalarial activity of subfractions was derived
from terpenoids substances.
i---1
= considered to contain terpenoids.
noids.
coNctustoN
lc,
Sub
ogr1
(rahl)
lm
l0
I
r)
0l
0.01
100
100
15.32
6J,III
56.19
0.0t2
B
1fi,
81.39
63.95
47 69
24.32
0.158
C
86.41
83 94
41.38
l5.l?
20.40
I -469
D
9690
92.48
64.81
15.5
26.40
o.t7z
E
loo
E5
?0
''t0.42
69_E4
56.75
0.m1
F
97.19
er.1r 68rr
59 8t
54.61
0.0t4
I
Table 1. lC50 values of Chloroform subfractions against P falciparum 307
Iti
i\\ltcl)|l
c
h
Subfraction E of A.acuminatissima Ieaves was
the most activ€ as antimalarialwith lC50value
of 0.007 uglml. The antimalarial activity was
possible derived from terpenoids substances.
Further study needed to isolate and identify
the active compounds from A.acuminatissima
ACKNOWLEDGEMENT
The authors acknowledge Satreps Lab-Natural
Product Medicine Research Development Division, lnstitute of Tropical Dlseases Universitas Airlangga for suporting this research.
RIFERENCES
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l:-------.--t --'---
K-\
I
BCD
d'
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