isolation and Selection of Extremophillic Cellulase Producing Bacteria Isolatedfrom Black Water Ecosystem
Mikrobiologi Indonesia. September 2000, hIm. 48-53
ISSN 0853-358X
Jurnal
Vol.5, No.2
Isolasi dan Seleksi Bakteri Penghasil Selulase Ekstremoffl dan
Ekosistem Air Hitam
isolation and Selection ofExtremophillic Cellulase Producing
Bacteria Isolatedfrom Black Water Ecosystem
FIKRINDAU, ISWANDI ANAS2, TRESNAWATI PURWADAIUA3 & DWI ANDREAS SANTOSA2*
`FakulMs Pertanian Universi.tas Syiah Kuala, Banda Aceh 23111
2Fakultas Pertanian, !nstiuS Pertanwn Bogor, Jalan Raya Pajajaran 1, Bogor 16144; dan
indonesian Centerfor Bi.odiversisy and Biotechnology, Jalan RE. Martadinata 8, Bogor
3Balai Penelilian Ternak Ciaw4 Bogor 16002
The cellulolytic bacteria are potential organisms for biotechnological applications such as in decomposition of
lignocellulose materials and enzyme technology. The black water ecosystem is a unique ecosystem because of its high
diversity of flora, fauna, and microbes. The aims of this experiment were to isolate cellulolytic bacteria from the
ecosystem of black water and to select their ability to grow at the extrem conditions low and high plls and high
temperature. The total number of cellulolytic bacteria isolated at 30°C with pH 4 or 7 and at 50°C with pH 4 or 7 was
1.263. In further screening at higher temperatures, 30 bacteria were obtained at 60°C while at 70°C only 16 bacteria
were selected. The isolates were furthter screened at different pH based on their optimal growth temperature, and
they were able to grow at pH 3-11. Besides bacteria that grow at normal temperature 4-40°C and at p11 neutral 5.8.5
there at high temperatures 60 and 70°C and low and high pils 3 and 10, 11 showing that there are high diversity in
the ecosystem of black water.
Key words: cellulolytic bacteria, black water ecosystem, extreme condition
Selulosa sebagai senyawa paling banyak di bumi tersusun
atas 8 000-12 000 unit glukosa yang dihubungkan oleh ikatan
-1,4-glukosida. Ikatan -l,4-glukosida pada serat selulosa
dapat dipecah menjadi monomer glukosa oleh selulase, yaitu
suatu sistem enzim yang terdiri atas tiga tipe enzim utama yaitu
endo- 1 A- -glukanase EC 3.2.1.1; ekso- 1,4- -glukanase EC
3.2.1.91 atau selobiohidrolase; dan f3 -D-glukosidase EC
3.2. 1.21.
Produksi selulase dalam teknologi enzim memerlukan
bakteri, aktinomiset maupun cendawan, yang menghasilkan
enzim ekstrasel. Meskipun berasal dan lingkungan normal
suhu >4°C atau 5 atau
ISSN 0853-358X
Jurnal
Vol.5, No.2
Isolasi dan Seleksi Bakteri Penghasil Selulase Ekstremoffl dan
Ekosistem Air Hitam
isolation and Selection ofExtremophillic Cellulase Producing
Bacteria Isolatedfrom Black Water Ecosystem
FIKRINDAU, ISWANDI ANAS2, TRESNAWATI PURWADAIUA3 & DWI ANDREAS SANTOSA2*
`FakulMs Pertanian Universi.tas Syiah Kuala, Banda Aceh 23111
2Fakultas Pertanian, !nstiuS Pertanwn Bogor, Jalan Raya Pajajaran 1, Bogor 16144; dan
indonesian Centerfor Bi.odiversisy and Biotechnology, Jalan RE. Martadinata 8, Bogor
3Balai Penelilian Ternak Ciaw4 Bogor 16002
The cellulolytic bacteria are potential organisms for biotechnological applications such as in decomposition of
lignocellulose materials and enzyme technology. The black water ecosystem is a unique ecosystem because of its high
diversity of flora, fauna, and microbes. The aims of this experiment were to isolate cellulolytic bacteria from the
ecosystem of black water and to select their ability to grow at the extrem conditions low and high plls and high
temperature. The total number of cellulolytic bacteria isolated at 30°C with pH 4 or 7 and at 50°C with pH 4 or 7 was
1.263. In further screening at higher temperatures, 30 bacteria were obtained at 60°C while at 70°C only 16 bacteria
were selected. The isolates were furthter screened at different pH based on their optimal growth temperature, and
they were able to grow at pH 3-11. Besides bacteria that grow at normal temperature 4-40°C and at p11 neutral 5.8.5
there at high temperatures 60 and 70°C and low and high pils 3 and 10, 11 showing that there are high diversity in
the ecosystem of black water.
Key words: cellulolytic bacteria, black water ecosystem, extreme condition
Selulosa sebagai senyawa paling banyak di bumi tersusun
atas 8 000-12 000 unit glukosa yang dihubungkan oleh ikatan
-1,4-glukosida. Ikatan -l,4-glukosida pada serat selulosa
dapat dipecah menjadi monomer glukosa oleh selulase, yaitu
suatu sistem enzim yang terdiri atas tiga tipe enzim utama yaitu
endo- 1 A- -glukanase EC 3.2.1.1; ekso- 1,4- -glukanase EC
3.2.1.91 atau selobiohidrolase; dan f3 -D-glukosidase EC
3.2. 1.21.
Produksi selulase dalam teknologi enzim memerlukan
bakteri, aktinomiset maupun cendawan, yang menghasilkan
enzim ekstrasel. Meskipun berasal dan lingkungan normal
suhu >4°C atau 5 atau