Scfa Profile Of Rice Rs Fermentation By Colonic Microbiota, Clostridium Butyricum Bcc B2571, Or Eubacterium Rectale Dsm 17629
SCFA PROFILE OF RICE RS FERMENTATION BY COLONIC
MICROBIOTA, Clostridium butyricum BCC B2571, or Eubacterium rectal
DSM 17529
DONALD JOHN CALVIEN HUTABARAT
GRADUATE SCHOOL
BOGOR AGRICULTURAL UNIVERSITY
BOGOR
2017
STATEMENT LETTER OF MANUSCRIPT AND SOURCE OF
INFORMATION*
I declare that this manuscript entitled SCFA Profile of Rice RS
Fermentation by Colonic Microbiota, Clostridium butyricum BCC B2571, or
Eubacterium rectal DSM 17529 is my own work with guidance of the advisors
and has not been submitted in any form at any college, except at Bogor
Agricultural University. Sources of information derived and quoted from
published and unpublished works of other authors mentioned in the text and
included in the reference chapter.
Herewith I bestow the copyright of this manuscript to Bogor Agricultural
University.
Bogor, January 2017
Donald John Calvien Hutabarat
F251124091
RINGKASAN
DONALD JOHN CALVIEN HUTABARAT. SCFA Profile of Rice RS
Fermentation by Colonic Microbiota, Clostridium butyricum BCC B2571, or
Eubacterium rectale DSM 17629. Supervised by FRANSISCA R. ZAKARIA
MAGGY T. SUHARTONO and ENDANG Y. PURWANI.
Pati resisten tipe 3 (RS3) yang dihasilkan dari berbagai sumber makanan
beramilosa tinggi melalui retrogradation atau proses enzimatik dikenal memiliki
fungsi fisiologis sebagai serat makanan. Fermentasi RS3 oleh mikrobiota kolon
menghasilkan SCFA (asetat, propionat, dan butirat), yang dapat menjaga
kesehatan usus, keseimbangan usus mikrobiota, mencegah penyakit radang usus
IBD) dan kanker kolon. RS3 dalam penelitian ini dibuat dari beras patah IR-42
dan Inpari-16 melalui perlakuan enzimatik (kombinasi amilase-pullulanase). Pati
resisten difermentasi selama 12 dan 24 jam dengan mikrobiota kolon,
Clostiridium butyricum BCC-B2571, atau Eubacterium rectale DSM 17629.
SCFA yang dihasilkan dianalisis dengan kromatografi gas.
Hasil penelitian menunjukkan bahwa setelah proses enzimatik, kadar RS3
dari IR-42 (41,13%) tidak berbeda nyata (p
MICROBIOTA, Clostridium butyricum BCC B2571, or Eubacterium rectal
DSM 17529
DONALD JOHN CALVIEN HUTABARAT
GRADUATE SCHOOL
BOGOR AGRICULTURAL UNIVERSITY
BOGOR
2017
STATEMENT LETTER OF MANUSCRIPT AND SOURCE OF
INFORMATION*
I declare that this manuscript entitled SCFA Profile of Rice RS
Fermentation by Colonic Microbiota, Clostridium butyricum BCC B2571, or
Eubacterium rectal DSM 17529 is my own work with guidance of the advisors
and has not been submitted in any form at any college, except at Bogor
Agricultural University. Sources of information derived and quoted from
published and unpublished works of other authors mentioned in the text and
included in the reference chapter.
Herewith I bestow the copyright of this manuscript to Bogor Agricultural
University.
Bogor, January 2017
Donald John Calvien Hutabarat
F251124091
RINGKASAN
DONALD JOHN CALVIEN HUTABARAT. SCFA Profile of Rice RS
Fermentation by Colonic Microbiota, Clostridium butyricum BCC B2571, or
Eubacterium rectale DSM 17629. Supervised by FRANSISCA R. ZAKARIA
MAGGY T. SUHARTONO and ENDANG Y. PURWANI.
Pati resisten tipe 3 (RS3) yang dihasilkan dari berbagai sumber makanan
beramilosa tinggi melalui retrogradation atau proses enzimatik dikenal memiliki
fungsi fisiologis sebagai serat makanan. Fermentasi RS3 oleh mikrobiota kolon
menghasilkan SCFA (asetat, propionat, dan butirat), yang dapat menjaga
kesehatan usus, keseimbangan usus mikrobiota, mencegah penyakit radang usus
IBD) dan kanker kolon. RS3 dalam penelitian ini dibuat dari beras patah IR-42
dan Inpari-16 melalui perlakuan enzimatik (kombinasi amilase-pullulanase). Pati
resisten difermentasi selama 12 dan 24 jam dengan mikrobiota kolon,
Clostiridium butyricum BCC-B2571, atau Eubacterium rectale DSM 17629.
SCFA yang dihasilkan dianalisis dengan kromatografi gas.
Hasil penelitian menunjukkan bahwa setelah proses enzimatik, kadar RS3
dari IR-42 (41,13%) tidak berbeda nyata (p