In vitro degradation of Acacia villosa by ruminal microbes of adapted and non-adapted sheep to acacia feeding
Jurnal Peternakan dan Lingkungan
Vol.09 No.2 ISSN : 0852 - 4092 Akreditasi Nomor : 134/Dikti/Kep/2001
4()
IN VITRO DEGRADATION OF ACACIA VILLOSA BY RUMINAL
MICROBES OF ADAPTED AND NON-ADAPTED SHEEP TO
ACACIA FEEDING
Komang G. Wiryawan·, Suryahadt and Anita S. Tjalcradidjaja·
Abstrak
Akasia berpotensi digunakan sebagai suplemen pakan karena kandungan
proteinllya cllkup tinggi (13-32% dari bahan kering), tetapi penggunaannYll dibatasi oleh
acianya alltin IItrisi dan toksin. Salah satu pendekatan yang dapat dilakukan lmtuk
menglIHlIlgi pengarllh negatif dari antinutrisi dan toksin tersebut adalah dt!ngan
menggunakan mikroba rumen dari temak yang secara alami sudah mengkonsums; ukasia.
Tujuan penelitian ini adalah untuk mengetahui kemampuan mikroba rumen dan ternak
yang sudah diadaptasi maupun yang belum diadaptasi dengan pakan akasia dalam
mendegradasi leguminosa Acacia villosa, Calliandra calothyrsus dan Leltc(lena
lellcocephala. Hasil penelitian menunjukkan bahwa ada perbedaan antara kemampuan
mikroba rllmen yang diadaptasi dengan akasia dibandingkan dengan yang tidak diadaptasi
dalam mendegradasi ketiga leguminosa tersebut. Akasia dan kaliandra kurang dapat
dicerna dibandingkan dengan lamtoro. Hal ini kemungkinan disebabkan adanya perbedaan
kandungan antinutrisi (tanin) yang berbeda dian tara ketiga leguminosa tersebut. Dari
hasil penelitian ini dapat disimpulkan bahwa temak yang diadaptasi dengan pakan akasia
mempunyai mikroba rumen yang toleran terhadap antinutrisi tanin yang ada pada akasia
dan membuka peluang dilakukannya isolasi terhadap mikroba tersebut.
Komang G. Wiryawan, S
Research Method
In Vitro Digestibility 1
Each legume leave
weight) was incubated
dia containing McOou
and each type of rum
different sheep. Rume
from the rumen of ｳｨｾ＠
cia from Kupang (Ku:
to acacia feeding fron
awi) and non-adapted
maga (Oarmaga). The
shed with CO 2 to maix:
tion, and then incubate
dability study, the inc
ted for 0, 3 and 6 h; al
and VF A production
measured. respective)
sion Conway and stt
matter (OM) and orgl
gestibilities were det
two-stage procedure
(1963); for these mea:
was carried out for 2 x
Statistical Analysis
A factorial 3x3 1
block design with t1
applied in the experiI
VF A productions wi
fluids and legumes, n
and II. A similar ex}:
Kata kllnci: akasia, adaptasi, antinutrisi, mikroba rumen
Introduction
Acacia sp. can be used as supplements
to improve ruminant production in Indonesia and other tropical areas due to its high
concentration of protein (13-32 % OM
basis) (Praptiwi, 1985; Tangendjaja and
Wina, 2000; Norton, 2000). However, its
utilization is limited by the presence of antinutritional and toxic compounds such as
tannins, toxic non-protein amino acids (2amino-4-acetytaminobutyric acid-ADAB;
2,4-diaminobutyric acid-OABA; and oxalylalbizzine), and mimosine (Ahn et al., 1989;
Evans et al., 1993).
Ruminants that naturally adapt to feed
containing the antinutrients are able to digest those legumes due to its rumen microorganisms have evolved to tolerate the
toxins. One approach to overcome the negative effects of antinutrients is by using
those rumen microbes. This approach was
evaluated in this experiment to obtain informations about the ability of rumen microbes of the adapted and non-adapted sheep to
acacia feeding to degrade Acacia vi/losa,
Calliandra calothyrsus and Leucael/a leucocephala.
Table I. Dry Matte
Rumen FIt
ｾ＠
ｴ ｾ
..ﾷ ｾ ｯＮ
_ _ __
Legumes
Acacia villosa
Cal/iandra calothyr:
Leucaenaleucocep/'
Staf Pengajar Fakultas Petemakan Institut Pertanian Bogor
Mean ± SE
Different superscript me
40
lYRUMINAL
,HEEPTO
n karena kandllngan
unaannya dibatasi oleh
tapal dilakllkan IlIItuk
·sebut adalah dt!l1gan
I mengkonsumsi (/kasia.
oba rumen dari lernak
l pakan akasia dalam
Iyrsus dan Lellcaena
Ian antara kemalllpuan
In yang tidak diadaptasi
aliandra kurang dapat
bkan adanya perbedaan
uminosa tersebllf. Dari
'si dengan pakal/ akasia
1 yang ada pada akasia
rbut.
naturally adapt to feed
lUtrients are able to didue to its rumen microvolved to tolerate the
ch to overcome the nentinutrients is by using
bes. This approach was
periment to obtain inforability of rumen microInd non-adapted sheep to
egrade Acacia \'illosa,
"sus and Leucael/a leu-
41
Komang G. Wiryawan. Suryahadi and Anita S. Tjakradidjaja
Research Method
In Vitro Digestibility Trial
Each legume leaves (1 g of dry-ground
weight) was incubated in three types of media containing McDougall solution (12 ml)
and each type of rumen fluid (8 ml) from
different sheep. Rumen fluid was obtained ·
from the rumen of sheep naturally fed acacia from Kupang (Kupang), sheep adapted
to acacia feeding from Balitnak-Ciawi (Ciawi) and non-adapted sheep from IPB-Darmaga (Darmaga). These mixtures were flushed with CO 2 to maintain anaerobic condition, and then incubated at 390 C. For degradability study, the incubation was conducted for 0, 3 and 6 h; ammonia concentration
and VF A production in supernatants were
measured, respectively using microdiffusion Conway and steam distillation. Dry
matter (DM) and organic matter (OM) digestibilities were determined following a
two-stage procedure of Tilley and Terry
(1963); for these measurements, incubation
was carried out for 2 x 24 h.
Statistical Analysis
A factorial 3x3 randomised complete
block design with three replications was
applied in the experiment of ammonia and
VF A productions with sources of rumen
fluids and legumes, respectively as factor I
and II. A similar experimental design was
also applied in DM and OM digestibility
study in which factor I was sources of rumen fluids and factor II was legumes. To
determine the effects of treatment, data
were analysed with analysis of variance
(Steel and Torrie, 1995).
Results and Discussion
Rumen fluid from three different animals differed in their abilities to degrade
the legumes, but the effects were influenced
by species of legumes and the incubation
period. The rumen fluid from the sheep naturally eat acacia (Kupang) degraded all the
legumes at a similar ,extent throughout the
incubation period. Acacia was degraded at a
lower extent than calliandra and leucaena
by the rumen fluid of adapted sheep from
Ciawi and by the rumen liquour of nonadapted sheep from Darmaga.
The results of in vitro I;>M and OM
digestibilities demonstrated that the rumen
fluid of Kupang was more capable (P
Vol.09 No.2 ISSN : 0852 - 4092 Akreditasi Nomor : 134/Dikti/Kep/2001
4()
IN VITRO DEGRADATION OF ACACIA VILLOSA BY RUMINAL
MICROBES OF ADAPTED AND NON-ADAPTED SHEEP TO
ACACIA FEEDING
Komang G. Wiryawan·, Suryahadt and Anita S. Tjalcradidjaja·
Abstrak
Akasia berpotensi digunakan sebagai suplemen pakan karena kandungan
proteinllya cllkup tinggi (13-32% dari bahan kering), tetapi penggunaannYll dibatasi oleh
acianya alltin IItrisi dan toksin. Salah satu pendekatan yang dapat dilakukan lmtuk
menglIHlIlgi pengarllh negatif dari antinutrisi dan toksin tersebut adalah dt!ngan
menggunakan mikroba rumen dari temak yang secara alami sudah mengkonsums; ukasia.
Tujuan penelitian ini adalah untuk mengetahui kemampuan mikroba rumen dan ternak
yang sudah diadaptasi maupun yang belum diadaptasi dengan pakan akasia dalam
mendegradasi leguminosa Acacia villosa, Calliandra calothyrsus dan Leltc(lena
lellcocephala. Hasil penelitian menunjukkan bahwa ada perbedaan antara kemampuan
mikroba rllmen yang diadaptasi dengan akasia dibandingkan dengan yang tidak diadaptasi
dalam mendegradasi ketiga leguminosa tersebut. Akasia dan kaliandra kurang dapat
dicerna dibandingkan dengan lamtoro. Hal ini kemungkinan disebabkan adanya perbedaan
kandungan antinutrisi (tanin) yang berbeda dian tara ketiga leguminosa tersebut. Dari
hasil penelitian ini dapat disimpulkan bahwa temak yang diadaptasi dengan pakan akasia
mempunyai mikroba rumen yang toleran terhadap antinutrisi tanin yang ada pada akasia
dan membuka peluang dilakukannya isolasi terhadap mikroba tersebut.
Komang G. Wiryawan, S
Research Method
In Vitro Digestibility 1
Each legume leave
weight) was incubated
dia containing McOou
and each type of rum
different sheep. Rume
from the rumen of ｳｨｾ＠
cia from Kupang (Ku:
to acacia feeding fron
awi) and non-adapted
maga (Oarmaga). The
shed with CO 2 to maix:
tion, and then incubate
dability study, the inc
ted for 0, 3 and 6 h; al
and VF A production
measured. respective)
sion Conway and stt
matter (OM) and orgl
gestibilities were det
two-stage procedure
(1963); for these mea:
was carried out for 2 x
Statistical Analysis
A factorial 3x3 1
block design with t1
applied in the experiI
VF A productions wi
fluids and legumes, n
and II. A similar ex}:
Kata kllnci: akasia, adaptasi, antinutrisi, mikroba rumen
Introduction
Acacia sp. can be used as supplements
to improve ruminant production in Indonesia and other tropical areas due to its high
concentration of protein (13-32 % OM
basis) (Praptiwi, 1985; Tangendjaja and
Wina, 2000; Norton, 2000). However, its
utilization is limited by the presence of antinutritional and toxic compounds such as
tannins, toxic non-protein amino acids (2amino-4-acetytaminobutyric acid-ADAB;
2,4-diaminobutyric acid-OABA; and oxalylalbizzine), and mimosine (Ahn et al., 1989;
Evans et al., 1993).
Ruminants that naturally adapt to feed
containing the antinutrients are able to digest those legumes due to its rumen microorganisms have evolved to tolerate the
toxins. One approach to overcome the negative effects of antinutrients is by using
those rumen microbes. This approach was
evaluated in this experiment to obtain informations about the ability of rumen microbes of the adapted and non-adapted sheep to
acacia feeding to degrade Acacia vi/losa,
Calliandra calothyrsus and Leucael/a leucocephala.
Table I. Dry Matte
Rumen FIt
ｾ＠
ｴ ｾ
..ﾷ ｾ ｯＮ
_ _ __
Legumes
Acacia villosa
Cal/iandra calothyr:
Leucaenaleucocep/'
Staf Pengajar Fakultas Petemakan Institut Pertanian Bogor
Mean ± SE
Different superscript me
40
lYRUMINAL
,HEEPTO
n karena kandllngan
unaannya dibatasi oleh
tapal dilakllkan IlIItuk
·sebut adalah dt!l1gan
I mengkonsumsi (/kasia.
oba rumen dari lernak
l pakan akasia dalam
Iyrsus dan Lellcaena
Ian antara kemalllpuan
In yang tidak diadaptasi
aliandra kurang dapat
bkan adanya perbedaan
uminosa tersebllf. Dari
'si dengan pakal/ akasia
1 yang ada pada akasia
rbut.
naturally adapt to feed
lUtrients are able to didue to its rumen microvolved to tolerate the
ch to overcome the nentinutrients is by using
bes. This approach was
periment to obtain inforability of rumen microInd non-adapted sheep to
egrade Acacia \'illosa,
"sus and Leucael/a leu-
41
Komang G. Wiryawan. Suryahadi and Anita S. Tjakradidjaja
Research Method
In Vitro Digestibility Trial
Each legume leaves (1 g of dry-ground
weight) was incubated in three types of media containing McDougall solution (12 ml)
and each type of rumen fluid (8 ml) from
different sheep. Rumen fluid was obtained ·
from the rumen of sheep naturally fed acacia from Kupang (Kupang), sheep adapted
to acacia feeding from Balitnak-Ciawi (Ciawi) and non-adapted sheep from IPB-Darmaga (Darmaga). These mixtures were flushed with CO 2 to maintain anaerobic condition, and then incubated at 390 C. For degradability study, the incubation was conducted for 0, 3 and 6 h; ammonia concentration
and VF A production in supernatants were
measured, respectively using microdiffusion Conway and steam distillation. Dry
matter (DM) and organic matter (OM) digestibilities were determined following a
two-stage procedure of Tilley and Terry
(1963); for these measurements, incubation
was carried out for 2 x 24 h.
Statistical Analysis
A factorial 3x3 randomised complete
block design with three replications was
applied in the experiment of ammonia and
VF A productions with sources of rumen
fluids and legumes, respectively as factor I
and II. A similar experimental design was
also applied in DM and OM digestibility
study in which factor I was sources of rumen fluids and factor II was legumes. To
determine the effects of treatment, data
were analysed with analysis of variance
(Steel and Torrie, 1995).
Results and Discussion
Rumen fluid from three different animals differed in their abilities to degrade
the legumes, but the effects were influenced
by species of legumes and the incubation
period. The rumen fluid from the sheep naturally eat acacia (Kupang) degraded all the
legumes at a similar ,extent throughout the
incubation period. Acacia was degraded at a
lower extent than calliandra and leucaena
by the rumen fluid of adapted sheep from
Ciawi and by the rumen liquour of nonadapted sheep from Darmaga.
The results of in vitro I;>M and OM
digestibilities demonstrated that the rumen
fluid of Kupang was more capable (P