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4.1.4.4 Effect on height and stem diameter of RKN free plants
The data indicates that all the tested endophytic fungi isolates generally showed significant increase in plant height every week during the four week period of
observation in comparison to the control treatments, the plant heights varied depending on the different species used, the most effective endophytic fungi were
Chrysosporium sp, Fusarium chlamydosporum, isolate XP 9, Nigrospora sp, Torula
sp, Trichoderma hamatum, Fusarium oxysporum and Sterile black 2 while the least significant species were Ulocladium sp, and Fusarium sp 3. Table 8 and appendix 3
On the same note all endophytic fungi isolates used showed significant effects in increasing stem diameter every week during the four week period of observation in
comparison to the control treatments, the stem diameter varied depending on the different species of endophytic fungi, the most effective endophytic fungi isolate in
increasing the plant diameter were Trichoderma pseudokoningii, Nigrospora sp, Torula
sp, Sterile black 1, isolate XP 9, Fusarium oxysporum and Trichoderma hamatum
while the least significant endophytic fungi in increasing stem diameter were Ulocladium sp, and Fusarium sp3 Table 9.
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Table 8. Effect of endophytic fungi on plant height of RKN free tomato plant Treatment
Mean Plant height cm 8 th Week
9 th Week 10 th Week
11 th Week Chrysosporium
sp 65.08 ± 2.81 a
69.92 ± 2.78 a 73.58 ± 2.64 a
77.33 ± 2.27 a Fusarium chlamydosporum
63.75 ± 3.84 ab 65.00 ± 3.02 bc
69.33 ± 2.96 b 73.25 ± 2.49 b
Isolate XP 9 63.00 ± 3.30 ab
69.00 ± 3.50 a 72.17 ± 2.95 ab
75.17 ± 3.16 ab Nigrospora
sp 62.92 ± 4.12 ab
69.50 ± 2.91 a 73.33 ± 2.27 a
77.17 ± 2.55 a Torula
sp 62.58 ± 4.25 ab
64.33 ± 2.81 c 66.42 ± 2.84 c
69.92 ± 2.58 c Trichoderma hamatum
62.25 ± 3.08 ab 64.25 ± 1.91 c
65.92 ± 2.23 c 68.83 ± 2.32 c
Fusarium oxysporum 61.33 ± 2.06 ab
60.83 ± 2.29 d 62.67 ± 1.61 d
65.75 ± 1.66 d Sterile black 2
61.17 ± 3.22 ab 63.08 ± 2.11 cd
65.17 ± 1.95 cd 69.75 ± 1.66 c
Sterile black 1 60.67 ± 3.63 b
62.83 ± 1.95 cd 65.00 ± 1.41 cd
68.83 ± 1.90 c Trichoderma pseudokoningii
60.25 ± 3.57 b 67.58 ± 3.15 ab
71.00 ± 2.13 ab 74.00 ± 2.37 b
Fusarium sp 3
41.92 ± 6.82 c 43.25 ± 6.33 e
46.00 ± 6.27 e 49.08 ± 5.80 e
Ulocladium sp
38.83 ± 8.04 c 39.33 ± 7.92 f
40.25 ± 7.63 f 41.17 ± 7.61 f
Control Without Endophytic fungi 24.08 ± 3.26 d
25.42 ± 2.75 g 26.58 ± 2.43 g
28.33 ± 2.61 g
Mean ± Standard deviation Means in the same column followed by the same letter are not significantly different at p0.05, according to Duncan’s Multiple Range Test
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Table 9. Effect of endophytic fungi on stem diameter of RKN free tomato plant Treatment
Mean stem diameter cm 8 th week
9 th week 10 th week
11 th week Trichoderma pseudokoningii
0.90 ± 0.13 a 0.90 ± 0.131 a
0.93 ± 0.13 a 0.95 ± 0.13 a
Nigrospora sp 0.84 ± 0.11 ab
0.87 ± 0.07 ab 0.90 ± 0.06 ab
0.93 ± 0.07 ab Torula sp
0.83 ± 0.09 ab 0.85 ± 0.08 abc
0.87 ± 0.08 abc 0.89 ± 0.08 abc
Sterile black 1 0.83 ± 0.08 ab
0.83 ± 0.08 abcd 0.85 ± 0.07 bcd 0.88 ± 0.08 abc
Isolate XP 9 0.83 ± 0.12 ab
0.83 ± 0.12 abcd 0.85 ± 0.12 bcd 0.87 ± 0.13 bcd
Fusarium oxysporum 0.83 ± 0.10 ab
0.83 ± 0.09 abcd 0.84 ± 0.07 bcd 0.86 ± 0.08 bcde
Trichoderma hamatum 0.82 ± 0.09 ab
0.82 ± 0.09 bcd 0.84 ± 0.08 bcd 0.86 ± 0.08 bcde
Chrysosporium sp 0.79 ± 0.06 b
0.80 ± 0.06 bcd 0.82 ± 0.06 cd 0.83 ± 0.06 cde
Fusarium chlamydosporum 0.78 ± 0.07 b
0.78 ± 0.07 cd 0.78 ± 0.07 d
0.79 ± 0.06 e Sterile black 2
0.76 ± 0.05 b 0.76 ± 0.05 d
0.78 ± 0.05 d 0.80 ± 0.05 de
Fusarium sp 3
0.57 ± 0.05 c 0.57 ± 0.05 e
0.57 ± 0.04 e 0.58 ± 0.05 f
Ulocladium sp 0.56 ± 0.04 c
0.56 ± 0.04 e 0.57 ± 0.05 e
0.58 ± 0.04 f Control Without endophytic fungi
0.48 ± 0.09 d 0.48 ± 0.09 f
0.48 ± 0.08 f 0.48 ± 0.08 g
Mean ± Standard deviation Means in the same column followed by the same letter are not significantly different at p0.05, according to Duncan’s Multiple Range Test
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4.1.4.5 Effect on plant fresh and dry weight of RKN free plants
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