41
4.1.4.5 Effect on plant fresh and dry weight of RKN free plants
The data indicated significant increase in plant fresh weight by endophytic fungi treatments, most effective treatments were Nigrospora sp, isolate XP 9 and
Trichoderma pseudokoningii, with significant mean plant fresh weight of 134.33
g, 131.33 g and 130.00 g respectively in that order, least mean plant fresh weights were observed in treatments with Fusarium sp3 and Ulocladium sp, that is 70.08 g
and 69.17 g respectively Table 10. The data also indicated significant increase in plant dry weight, most
effective treatments were Nigrospora sp, isolate XP 9 and Trichoderma pseudokoningii
with the highest mean dry weight of 43.91 g, 41.50 g. and 41.33 g, respectively in that order, least dry weights were observed in treatments with
Fusarium sp3 and Ulocladium sp, that is 18.67 cm and 15.67 g respectively
Table 10. Table 10. Effect of endophytic fungi on plant fresh and dry weight
Treatment Mean fresh weight Mean dry weight
Nigrospora sp
134.33 ± 14.75 a 43.91 ± 2.15 a
Isolate XP 9 131.33 ± 18.15 ab
41.50 ± 1.93 ab Trichoderma pseudokoningii
130.00 ± 19.98 ab 41.33 ± 3.70 ab
Fusarium oxysporum 121.33 ± 15.62 b
39.08 ± 3.62 bc Sterile black 1
107.25 ± 10.95 c 36.17 ± 4.76 c
Trichoderma hamatum 106.50 ± 15.05 c
37.92 ± 4.50 bc Torula
sp 104.08 ± 11.21 c
37.50 ± 4.54 c Chrysosporium
sp 103.83 ± 15.49 c
38.67 ± 3.60 bc Sterile black 2
102.17 ± 4.46 c 36.08 ± 4.54 c
Fusarium chlamydosporum 97.92 ± 17.05 c
38.17 ± 4.69 bc Fusarium
sp 3 70.08 ± 4.46 d
18.67 ± 4.70 d Ulocladium
sp 69.17 ± 4.28 d
15.67 ± 4.44 d Control Without endophytic fungi
27.58 ± 1.78 e 11.25 ± 2.83 e
Mean ± Standard deviation Means in the same column followed by the same letter are not significantly different at p0.05,
according to Duncan’s Multiple Range Test
4.1.4.6 Effect on number of fruits and root length RKN free plants
The data on mean number of fruits indicated higher significant increase in number of fruits by Nigrospora sp, isolate XP 9, Trichoderma pseudokoningii,
Trichoderma hamatum, and Chrysosporium sp with mean number of fruits
42 of 12.42, 12.42, 12.25, 12.00 and 11.83 respectively in that order, least mean
number of fruits were observed in treatments with Ulocladium sp, and Fusarium sp 3 that is 4.00 and 3.92 respectively.
The data also indicates that all endophytic fungi isolates used were highly significant in increasing root length development in comparison to the control
treatments, whereby Nigrospora sp, isolate XP 9, Trichoderma pseudokoningii had the highest mean root length of 41.58 cm, 41.00 cm and 39.42 cm
respectively, least root length was observed in treatments with Ulocladium sp, and Fusarium
sp3 that is 17.83 cm and 16.25 cm respectively Table 11. Table 11. Effect of endophytic fungi on number of fruits and root length
Treatments No. of fruits
Root length cm Nigrospora
sp 12.42 ± 1.44 a
41.58 ± 1.96 a Isolate XP 9
12.42 ± 2.43 a 41.00 ± 1.93 ab
Trichoderma pseudokoningii 12.25 ± 1.66 a
39.42 ± 3.70 abc Trichoderma hamatum
12.00 ± 1.86 ab 36.92 ± 4.15 cde
Chrysosporium sp
11.83 ± 1.90 abc 37.50 ± 4.50 bc
Torula sp
10.42 ± 2.16 bcd 33.50 ± 4.58 e
Fusarium chlamydosporum 10.08 ± 2.64 cd
38.25 ± 3.63 abcd Sterile black 2
10.08 ± 2.58 cd 33.42 ± 4.54 e
Sterile black 1 10.00 ± 2.70 d
34.75 ± 5.68 de Fusarium oxysporum
8.92 ± 2.28 d 38.75 ± 6.26 abc
Ulocladium sp
4.00 ± 1.41 e 17.83 ± 4.70 f
Fusarium sp 3
3.92 ± 1.31 e 16.25 ± 4.79 f
Control Without endophytic fungi 1.42 ± 1.31 f 8.92 ± 2.43 g
Mean ± Standard deviation Means in the same column followed by the same letter are not significantly different at p0.05,
according to Duncan’s Multiple Range Test
4.1.5 Antibiosis In vitro Test
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