-0.30 DMT1 0.52 -0.58 0.50 0.84 0.64 0.32 0.34 0.272 The leaf numbers and root diameter of Pule Pandak (Raufolvia serpentina Benth.) influence the root-dry weight and reserpine content.

ISSN: 2087-0183 33 The increase of air humidity would increase the soil water and decrease the soil temperature. Monteith et al. 7 stated that the shading increases the efficiency of water used by the plants below, by decreasing the evapo- transpiration of the plants below. The soil chemical character of those three research location were not so much different and it could be categorized into less fertile soil Table 4. Soil in Tekil, Sawangan and Saradan were latosol. NPK content and organic substances in those three research field showed the low availability of the elements. However, KTK in Saradan was higher than that of in Tekil and Sawangan. Table 2. Correlation coefficient of Pule Pandak from Tekil forest habitat TT1 JMD1 LSD1 PA1 JA1 DMT1 BKA1 JMD1 -0.38 LSD1 -0.64 0.73 PA1 0.35

0.27 -0.30

JA1 -0.04 -0.04 -0.30

0.55 DMT1

0.11 0.52

0.35 -0.58

-0.50 BKA1

0.35 0.50

0.06 0.84

0.08 0.64

Rsp1 0.07

0.05 0.32

0.19 0.34

0.78 0.272

Note: TT = height of plant; JMD = number of leaf; LSD = width of leaf; PA = root’s length; JA = number of root; DA = root’s diameter; BKA = root’s dry weight; RSP = Reserpine Table 3. The environment variable on research location Location Place Height m asl Rainfall myrs Climate Type Light Intensity fc Air Temperatur e ˚C Air Humidity Soil Water Tekil 400 1.966.40 C 11.873 27.46 77.67 36.48 Sawangan 790 2980.50 C 10.928 24.37 88.44 39.18 Saradan 435 1854.45 C 12.870 27.64 78.24 38.48 Table 4. Analysis of soil sample on research location Soil Character Tekil Sawangan Saradan Chemical Characters Total N 0.31 0.48 0.43 Provided P ppm 5.53 16.23 11.76 Exchanged K me100g 0.18 0.19 0.24 Ca me100g 19.25 10.42 19.48 KTK me100 g 18.60 19.37 30.15 Organic matter 3.05 4.02 3.39 PH 5.92 5.46 5.93 Textures: dust 39.20 32.43 33.07 clay 42.36 52.34 52.76 silk 18.44 15.23 14.17 Water capacity 44.96 Latosol 43.77 Latosol 42.37 Latosol Generally, the dose of the organic fertilizer had an effect on the entire growth and yield parameter in Sawangan and Saradan but of the plant height, root length and reserpina in Sawangan Table 5.The increase of the root result was not always enclosed by the increase of secondary metabolite reserpine Figure 1. 34 Herms and Mattson 8 stated that there is a dilemma for the plant growth and the alkaloid forming. To stimulate the forming of defense function like the secondary metabolite it would needs an environment pressure condition. However, enough fertilization and soil water is able to increase the alkaloid production 9 , therefore the fertilization could affects the reserpine amount as proved by the present data. We found a direct relationship between the leaf number and the root dry weight r = 0.49. The increase of the leaf number was followed by the increase of the root dry weight Table 6. Tabel 5. Analysis of variance of growth and yield parameter of Pule Pandak KT Location TT JMD LSD PA JA DA BKA RSP Sawangan P ns ns ns Saradan P Note: P= Fertilization; TT = height of plant; JMD = number of leaf; LSD = width of leaf; PA = length of root; JA = number of root; DA = diameter of root; BKA = dry weight of root; RSP = reserpine Figure 1. Correlation of the fertilizer doses with the dry weight of the root and the reserpina content of Pule Pandak Table 6. The cross – component correlation of growth and root with dry weight of root Sawangan Parameter JMD LSD PA JA DA r BKA JMD

0.49 -0.14