Identification and characterization of genes induced during sexual differentiation in schizosaccharomyces pombe
Identification and Characterization of Genes Induced During Sexual
Differentiation in Schizosaccharomyces pombe
Shusei Sato1, 4, Hideki Suzuki2, Utut Widyastuti1, 3, Yasuo Hotta1 and Satoshi Tabata1, 4
(1) Department of Biology, School of Science, Nagoya University, Furoh-cho, Chikusa-ku, 46401 Nagoya, Japan; (2) Research Institute for Biological Sciences, Ajinomoto Co. LTD, Maedacho, Totsuka-ku, 244 Yokohama, Japan; (3) Department of Biology, Faculty of Mathematics and
Science, Bogor Agriculture Institute, Pajajaran, 16143 Bogor, Indonesia; (4) Present address:
Kazusa DNA Research Institute, 473-2 Okanazawa Midori-ku, 266 Chiba, Japan
Received: 25 October 1993; Accepted: 13 December 1993
Abstract
Five cDNA clones, harboring genetic messages preferentially expressed during the sexual
differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by
subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was
dependent on nitrogen starvation and their induction occurred at several stages of spore
formation. Analysis of the cDNA prmary structures revealed a capacity for the coding of
polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The
translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to
those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively.
Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect
on spore formation.
Keywords: Schizosaccharomyces pombe-Sexual differentiation-Stage-specific genes Subtractive
screening
http://www.springerlink.com/content/x414388018544382/
Differentiation in Schizosaccharomyces pombe
Shusei Sato1, 4, Hideki Suzuki2, Utut Widyastuti1, 3, Yasuo Hotta1 and Satoshi Tabata1, 4
(1) Department of Biology, School of Science, Nagoya University, Furoh-cho, Chikusa-ku, 46401 Nagoya, Japan; (2) Research Institute for Biological Sciences, Ajinomoto Co. LTD, Maedacho, Totsuka-ku, 244 Yokohama, Japan; (3) Department of Biology, Faculty of Mathematics and
Science, Bogor Agriculture Institute, Pajajaran, 16143 Bogor, Indonesia; (4) Present address:
Kazusa DNA Research Institute, 473-2 Okanazawa Midori-ku, 266 Chiba, Japan
Received: 25 October 1993; Accepted: 13 December 1993
Abstract
Five cDNA clones, harboring genetic messages preferentially expressed during the sexual
differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by
subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was
dependent on nitrogen starvation and their induction occurred at several stages of spore
formation. Analysis of the cDNA prmary structures revealed a capacity for the coding of
polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The
translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to
those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively.
Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect
on spore formation.
Keywords: Schizosaccharomyces pombe-Sexual differentiation-Stage-specific genes Subtractive
screening
http://www.springerlink.com/content/x414388018544382/