Pewarisan sifat ketahanan cabai merah terhadap cucumber mosaic virus (CMV) dan analisis keterpautan marka RAPD dengan gen ketahanan terhadap CMV

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ELNANTI. Inheritance of Cucumber mosaic virus (CMV)-Resistance

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Characters in Hot Peppier (Capsicum annuum L.) and A Genetic Linkage analysis
between RAPD Markers and CMV-Resistance Gene(s). Supemsing committee :
SUDARSONO (chairman), RUSMILAH SUSENO and HAJRIAL
ASWII)lNNOOR (members).

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Information regarding the gene@) controlling CMV resistance and its
inheritance is important for breeding CMV resistance in hot pepper. CMV is the
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most widwspread virus infecting hot pepper in Indonesia, and its infection has
$ caused major yield reduction. Cumntly, such information is limitted, especially
3. for commercially grown hot pepper in Iqdonesia. Therefore, fbrther studies on
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these subject are necessary, The allelism study can be detected through crossing
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among CMV-resistance genotypes, and this information is related to breeding
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UI strategy to develop high-yielding and CMV-resistance hot pepper cultivars. The
use of hybrid genotype is one of the alternatives in increasing the national
g production of hot pepper. However, the limited availability of local hybrid has
5 caused our pepper producers rely on and suffer from the high price of imported
2 seeds. For this reason, the development of local hybrid of hot pepper was done.
2J Molecular marker such as RAPD (Random Amplified Polymorphic DNA) has
r been used to tag certain desirable gene(s) in the plant genome by genetic linkage

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This research consisted of four experiments; (1) Inheritance of Cucumber
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-4 mosaic virus (CMV) resistance characters in hot pepper. This experiment aimed
to study the action of gene(s) controlling the CMV-resistance in hot pepper, to
estimate the number of resistance genes, and to estimate the heritability values.
(2) The allelism among genes associated with CMV resistance in three CMVresistance genotypes of hot pepper. This research aimed to study the allelism
among genes associated with CMV resistance from different donor parents. (3)
Combining ability and heterosis in developing hybrids of hot pepper. This
research aimed to investigate the growth and productivity, general and specific
combining ability (GCA and SCA), and heterosis in order to develop the highyielding and CMV-resistance in hot pepper and (4) Linkage analysis between
RAPD markers and CMV-resistance gene(s) in hot pepper using bulk segregant
0 analysis, that aimed to develop genetic linkage analysis and identi@ specific
a RAPD marker linked to CMV resistance in hot pepper.
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The experiments used nine sets of crossing between three CMV-resistance
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(PBC 375, PBC 398 and PBC 535) and three CMV-susceptible genotypes (PBC
0 378, PBC 1354 and Rawit). The analysis was done using Mendelian genetic test,

I. by comparing the ratio of phenotypic frequency on segregated F2 population
0 against Mendelian ratio through Chi-square test, The identification of
c polymorphic RAPD marker for CMV resistance using Bulk Segregant Analysis
(BSA) was conducted by amplifying DNA in resistance and susceptible pools
using a number of random primers. Resistance and susceptible DNA pools w e n
obtained by mapping the segregated population (F2) resulted from crossing
C between resistance parent "PBC 375" and susceptible parent "Rawit".
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