as carrier of different materials like hormones, fatty acids, and drugs in the blood. Also, because it holds approximately 18 mL of water per gram of HSA, it is used as a plasma
volume expander in the treatment of hemorrhage or shock through infusions. It is also used as a protein replacement in cases of very low protein levels in the serum. But there
are also other forms of albumin other than HSA, and are found in many other animals. Bovine serum albumin BSA, for example, is found in the blood of cows. Its primary
biological function has been associated with its lipid binding properties. BSA is cheap and plentiful due to the fact thats it is a natural byproduct of the cattle industry. It is often
used in restriction digestion to stabilize some specific enzymes during the digestion of DNA for studies, making it ideal for determining the quantities of other particular
proteins present. It is also often used in cell generation, e.g. regenerating plants from cultured guard cells, by feeding the cell a healthy amount of protein leading to faster
growth and improved healing. BSA is quite stable, which makes it ideal for scientific
measures, in vaccine production, medical research, and food additives.
In this study, the number of platelets of thrombocytopenic Mus musculus white mice will be determined after the infusions of bovine serum albumin. The BSA will be
experimented at different concentrations of BSA.
B. Objectives of the Study
This study aims to determine the percent increase in the number of platelets of thrombocytopenic Mus musculus white mice after the infusions of bovine serum
albumin.
Specifically, it aims: 1.
To compare the number of platelets in number of platelets per μL of the 1 thrombocytopenic and 2 normal white mice a before and b after the BSA
infusions 2.
To compare the percent increase in the number of platelets of the thrombocytopenic and normal white mice after the BSA infusions
C. Hypotheses of the Study
There will be a significant difference in the number of platelets of thrombocytopenic white mice and will increase in number after the BSA infusions.
D. Significance of the Study
The use of another type of albumin, bovine serum albumin, which is homologous to the human serum albumin, will be tested on the Mus musculus white mice to find its
capability in increasing the number of platelets of thrombocytopenic white mice. Thrombocytopenia is one of the major symptoms of Dengue Hemorrhagic Fever
DHF. In 2010, the number of cases has risen drastically in the Philippines. Platelet transfusions are done to treat some cases of DHF in order to increase the number of
platelets, which is gravely affected by the disease. However, post-complications are commonly present in patients who underwent platelet transfusions. Compatibility issues,
refractoriness, red cell alloimmunization, fever, granulocytopenia, and post-transfusion purpura were some of the complications present after platelet transfusion, as mentioned
in a review done by Kelton 1979 and in the Editorials of the New England Journal of
Medicine entitled The Perils of Platelet Transfusions 1997. Also, the high cost of the procedure was mentioned in the reviews.
Fortunately, it is known that the use of albumin transfusion, using fractionized human serum albumin, is acceptable to treat patients with shock and hemorrhage
– the loss of blood from the circulatory system by leaking through damaged or cut blood
vessels in the body. These continuous bleedings may be prolonged by issues concerning malfunctions in platelets, like increased destruction of platelets in the circulatory system.
However, fractionized human serum albumin is easily the most expensive volume expander ―Mollison‘s Blood Transfusion in Clinical Medicine‖, Klein and others 2005.
Still, this procedure is usually done instead of platelet transfusion. The present study aims to test the capability of bovine serum albumin in increasing the number of platelets in the
body when infused by determining the percent increase in the number of platelets of thrombocytopenic white mice after the infusions of bovine serum albumin.
E. Scope and Delimitation
