61
3.1.2 Reagents Used
3.1.2.1 Potassium Permanganate KMnO
4
Solution
KMnO
4
solution was used for the determination of un-reacted H
2
O
2
during UVH
2
O
2
oxidation process. The standard KMnO
4
solution was prepared by dissolving 4 gram of KMnO
4
into 1 liter of distilled water, which was standardized using 0.1 N sodium oxalate solution [124].
3.1.2.2 HPLC Mobile Phase
Two mobile phases were used in the HPLC analysis i.e. mobile phase for YMC-Pack PolymerC18 column and mobile phase for Transgenomic column. The HPLC mobile
phase for YMC-Pack PolymerC18 column was a mixture of 0.1 M Na
2
HPO
4
and 0.1 M NaOH solutions in water. Na
2
HPO
4
solution 0.1 M was prepared by
dissolving 14.19 grams of Na
2
HPO
4
into 1 liter of distilled water, while 0.1 M NaOH was prepared by dissolving 4 grams of NaOH into 1 liter of distilled water. The ratio
of the mixture used was 60 of Na
2
HPO
4
and 40 of NaOH at pH of 12. The mobile phase for Transgenomic column used was 0.01 N H
2
SO
4
, which was prepared by dissolving 0.275 ml of 98 H
2
SO
4
in 1 liter of distilled water. Before use, the stock solutions were filtered
using paper filter of 0.45 m pore diameter. This filtration was conducted to eliminate the presence of solid particles in the stock solution, which will
create disturbancesuncertainties during HPLC analysis.
3.1.2.3 Calcium Hydroxide CaOH
2
Solution
Generally, water H
2
O and carbon dioxide gas CO
2
are produced duringend of the oxidation of organic compound. Identification of CO
2
liberated during UVH
2
O
2
oxidation process of MDEA was conducted using CaOH
2
. The CaOH
2
solution
62 was prepared by dissolving 1.5 grams of CaOH
2
into 1 liter of distilled water and kept overnight for allowing the solid particle to settle down and the resulted clear
solution from the mixture was used to absorb the liberated CO
2
into the solution.
3.1.2.4 Sodium Hydroxide NaOH Solution
Sodium hydroxide 1 M NaOH solution was prepared by dissolving 40 gram of NaOH into 1 liter of distilled water. Since the sample was a strong solution, a
concentrated NaOH solution was used for initial pH adjustment.
3.1.2.5 Sulphuric Acid H
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