Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 Paper ISSN 2225-093X Online Vol 3, No 3
2013
3 | P a g e
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Indigenous Vesicular Arbuscular Mycorrhizal VAM Fungi in Cashew nut
Anacardium occidentale
L. Plantation of North East-Bali Island - Indonesia
Meitini W. Proborini
1+
, Made Sudana
2
, Wayan Suarna
3
, N. P. Ristiati
4
1. Post Graduate student of Agriculture faculty and
+
Department of Biology, Math and Nature Science Faculty Udayana University, Campus Bukit Jimbaran Bali
Indonesia 2. Faculty of Agriculture, Udayana University, Campus Soedirman Denpasar Bali Indonesia
3. Faculty of Husbandery, Udayana University, Campus Soedirman Denpasar Bali Indonesia 4. Ganesha Education University, Campus Singaraja Bali Indonesia
E-mail of the corresponding author: pmeitiniyahoo.com
The resea rch is financed. by Directorate General of Higher Education, Ministry of Education and Culture of Republic Indonesia grant of Doctorate student and University of Udayana Bali.
Abstract
A study on indigenous VA-mycorrhizal fungi associate with “cashew nut” plants
anacardium occidentale
L. was carried out at arid areas of North-East of Bali Indonesia from April 2011 up to February, 2012 representing dry and rainy seasons consecutively.
Soil samples were randomly collected from rhizosphere of either seedling or adult cashew-nut plants with 2 months interval. Spores of myccorhizal fungi were extracted from the soil by wet-sieving and
decanting method and morfologically identified. This study found 5 genera consisiting of 13 species i.e.
Acaulospora
4 species,
Gigaspora 2 species, Scutellospora
1 species
, Entrophospora
1 species and
Glomus
5 species. The most frequent genera found was
Glomus
and the least was
Entrophospora
. The number of spore varied temporally and showed opposite trend to the monthly total of rain fall. The average of spore number was 52 to 91 per 250
g soil sample.
Keywords: Arbuscular mycorrhizae,
A. occidentale
L
.
Arid area, Bali
Island
1. Introduction
North-East region of Bali island is one of the three dry areas in Bali. The region is characterised by sandy soil structure thought it has been washed away since it erupted at 1960s, lack of water and
extreme temperature during dry season. All those lead to low productivity farming due to limitation of soil water Antara, 2004 ; Aditya,2011. The local people relies onto the limited farming during rainy
season and cashew nut plantation that grow and adapted to such condition. Currently The cashew farming, however, is facing a serious problem caused by infection of
Rhidigosporus
sp “white root fungi” and
Phytopthora sp
Proborini, unpublished. In accordance with the potential of VAM fungi as biofertiliser for plant suggested by many
studies and organic farming program promoted by the Government of Bali province, VAM fungi has been applied and developed for plant farming. The premises were that Vesicular Arbuscular Mycorrhizal VAM
fungi are abundant in almost all natural terrestrial communities and form obligate symbiotic associations with over than 80 of vascular plants Smith
et al
, 2010. VAM- fungi takes roles in maintaining ecosystem processes by promoting plant fitness through a range of mechanisms Brundrett
et al
, 2008; protecting plant host from soil pathogens Smith, 2000 and improving soil structure, enhancing water and
Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 Paper ISSN 2225-093X Online Vol 3, No 3
2013
4 | P a g e
www.iiste.org nutrient uptake Smith
et al
. 2010; increase the efficiency of fertilizer use and plant growth Douds
et al
. 2010.
Regarding those above, some efforts have been undertaken to raise up farming productivity in Bali by applying michorizal fungi on nut and corn farming in field as a pilot project Sukasta and Proborini ,
unpublished data .
The result was not optimum yet which might be due to either less adapted mycorrizhal spore or ineffective spore dose. Similarly goverment regional technical assistance unit of Bali province is
undergoing a research to diminish the impact of “ white root disease “. These efforts applied commercial
mycorrhizal fungi explored from other region, instead of indigenous mycorhizae .
The indigenous michorizal spore, however, has not been developed yet. The other part of this study showed that local plants
at North-East Bali were highly associate with VAM fungi indicated by hyphal infection in plant root. Therefore it is urgent to explore the diversity of indigenous micorizha in Cashew plantation rizosphere.
2. Material and Methods
