UJI AKTIVITAS ANALGESIK EKSTRAK ETANOL 80% DAUN Marsilea crenata Presl. DENGAN METODE GELIAT PADA MENCIT Repository - UNAIR REPOSITORY

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  LAMPIRAN LAMPIRAN I Tabel Konversi Dosis Hewan Uji dengan Manusia (Laurence, 2008)

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  3

  2

  2 = = = ଼଴ ଼଴ ଼଴ ି ି ି ଶଷ ଶଷ ଶଷ

  ଼଴ ଼଴ ଼଴ š š š š ͳͲ ͳͲ ͳͲ Ͳ Ͳ ͲΨ ͲΨ ͲΨ ͲΨ Ͳ ൌ ൌ ൌ ͳ ͳ ͳ ͳ ͳ ǡ ʹͷ ʹͷ ʹͷ ʹ Ψ Ψ Ψ Ψ

  % Pr r ot ot ot ot o ek ek ek k ek ek si si si si i s Ke Ke Ke Ke K K lo lo o lo lo lo mp mp mp mpok ok ok ok Ek Ek Ek Ek Ek E st st st t ra r rak k k k k Do Do Do si si si si s s s s

  3

  3

  3 3 = = = ଼଴ ଼଴ ଼଴ ଴ି ି ି ି ଵ ଵଽ ଵଽ ଽ ଵ ଽ ଼ ଴ ଼଴ ଼ ଼଴

  ʹǡ ǡ ͷͲ ͷ ͷͲ Ͳ ͷͲ ͷͲ Ψ Ψ Ψ Ψ

  š š š š ͳͲ ͳͲ ͳͲ ͳͲ ͳͲ Ͳ ͲΨ ͲΨ Ψ ͲΨ ͲΨ ൌ ͸ǡʹͷΨ Ψ Ψ Ψ Ψ

  % ProteksiKelomp mp p p p ok ok ok ok o Ek Ek E Ek Ek k st st st st ra ra ra r kD kD kD k k os os os o o is is s i i

  4

  4

  4 4 4 = = = ଼଴ ଼଴ ଼଴ ଼଴ ଴ ି ି ି ି ଵଷ ଵଷ ଵଷ ଵଷ ଷ

  ଼଴

  š ͳͲͲΨ ൌ

  % Pr Pr Pr r r r ot o ot o ek ek ek ek ek ek si si si s Ke Ke Ke Kelo lo lo lo mp mp mp p ok ok ok Ek Ek Ek Ek s st s rak k k k Dos si s s 2

  LAMPIRAN II Perhitungan Persentase Proteksi

  % Proteksi Kontrol Positif =

  š ͳͲͲΨ ൌ ͹͸ǡʹͷΨ % ProteksiKelompokEkstrakDosis 4 =

  ଼଴ିଵଽ ଼଴

  š ͳͲͲΨ ൌ ͹͸ǡʹͷΨ % Proteksi Kelompok Ekstrak Dosis 1 =

  ଼଴ିଷ଴ ଼଴

  š ͳͲͲΨ ൌ ͸ʹǡͷͲΨ % Proteksi Kelompok Ekstrak Dosis 2 =

  ଼଴ିଶଷ ଼଴

  š ͳͲͲΨ ൌ ͹ͳǡʹͷΨ % Proteksi Kelompok Ekstrak Dosis 3 =

  ଼଴ିଵଽ ଼଴

  ଼଴ିଵଷ ଼଴

  1 = = = =

  š ͳͲͲΨ ൌ ͺ͵ǡ͹ͷΨ

  % Proteksi = Rata-rata geliat kelompok kontrol negatif – Rata-rata geliat kelompok uji × 100% Rata-rata geliat kelompok kontrol negatif

  % Proteksi Ko Ko Ko o o nt nt nt tt ro ro ro ro l l l l l l Po Po Po P P o si si si i ti ti ti i f f f = = = =

  ଼଴ ଼ ଼ ି ି ଵ ଵଽ ଵ ଼ ଼ ଼଴ ଼ ଼ ଴

  š š š š š ͳͲ ͳͲ ͳͲͲΨ ͲΨ ͲΨ Ψ ͲΨ Ψ ൌ ൌ ൌ ൌ ൌ ͹͸ ͹ ͹ ͹ ǡʹͷΨ % Pr r r ot ot ot t ot ek ek ek ek si si si i Ke Ke Ke K lo lo lo lo o mp mp mp pok ok ok ok k k k Ek Ek Ek Ek Ek k Ek st st st st ra ra ra ra k k k k Do Do Do Do si si si sis s s s

  1

  1

  ଼଴ ଼ ଼଴ ଴ ଼ ି ି ି ି ଷ଴ ଷ଴ ଷ଴ ଼଴ ଼଴ ଼଴ ଼ š š š š ͳͲ ͳͲ ͳͲ ͳ Ͳ ͲΨ ͲΨ ͲΨ ͲΨ ͲΨ ൌ

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  1

  4 4 = = = ଻ହΨ

  4

  4

  š š š š ͳͲ ͳͲ ͳͲ ͳͲ ͳͲ ͲΨ ͲΨ ͲΨ Ψ ͲΨ ͲΨ ൌ ൌ ൌ ൌ ൌ ͳͲ ͳͲ Ͳ Ͳ ͳͲ ͳͲ ͳͲ ͳͲ ͳ ͲΨ ͲΨ ͲΨ ͲΨ % Efektivitas Ke e lo lo lo o o mp mp mp m m ok ok ok ok k Ek Ek Ek Ek st st st s ra ra ra a k k k k k Do Do Do o Do si si i si s s s s

  3 = = = = ǡଶହΨ Ψ ǡଶହΨ

  3

  3

  š ͳͲ ͳͲ ͳͲ ͳͲ ͳ ͲΨ ͲΨ Ψ ͲΨ ൌ ൌ ൌ ͻ͵ ͻ͵ ͻ ͻ͵ ǡ ͺͷ ͺͷ Ψ Ψ Ψ % Ef Ef f f ek ek ek ek k ti ti ti t i vi vi vi v v ta ta ta a a s s s s Ke Ke K K Ke K K lo lo lo l mp mp mp m ok ok ok ok Ek Ek Ek E st st stra ra a k k k Do Do D Dosi si si i s s s

  2 2 = = = ǡ ǡ ଶହΨ ଶହΨ ଶହΨ ଶହ ଶ ହ ǡ ଶହΨ ଶ ହΨ ଶହΨ ଶହΨ

  % % % Ef Ef Ef Ef Ef E ek ek ek ek ti ti ti ti t vi vi vi vi i v ta ta a a s s ss Ke Ke Ke Ke lo lo lo lo mp mp mp m ok ok ok ok k E E Ek E E stra r r k D D Do D si i ii s 2

  š š š ͳͲ ͳͲ ͳͲ ͲΨ ͲΨ ͲΨ Ͳ Ψ Ͳ Ψ Ψ Ψ ൌ ൌ ൌ ൌ ൌ ͺͳ ͺ ͺ ǡ ǡͻ ͻ͹ ͻ ͻ ͻ Ψ Ψ Ψ Ψ Ψ Ψ Ψ

  1 = = = ǡହ଴Ψ ଴Ψ ଴Ψ ଴Ψ ǡଶହΨ ଶହΨ ହΨ ହΨ

  1

  LAMPIRAN III Perhitungan Persentase Efektivitas

  % Efektivitas Kontrol Positif =

  ଻଺ ଺ ଻଺ ଻଺ ଻ ǡ ଶହΨ ଶହΨ ଶହΨ ଶହΨ Ψ ଻଺ ଺ ଺ ଺ ǡ ǡ ଶହΨ ଶହΨ ଶହΨ Ψ ଶହΨ ହΨ ଶହΨ š š š ͳͲ ͳͲ ͳͲ ͳͲ Ͳ Ͳ ͲΨ Ψ ͲΨ ͲΨ ͲΨ ൌ ͳͲͲΨ

  % Efektivitas s s Ko Ko Ko Ko nt nt nt nt nt t ro ro ro ro ro l l l Po Po Po P P s si si s ti ti ti ti f f f f = = = =

  % Efektivitas = % Proteksi Kelompok Uji × 100%

% Proteksi Kontrol Positif

  š ͳͲͲΨ ൌ ͳͲͺǡʹ͵Ψ

  ଼ଷǡ଻ହΨ ଻଺ǡଶହΨ

  š ͳͲͲΨ ൌ ͳͲͲΨ % Efektivitas Kelompok Ekstrak Dosis 4 =

  ଻଺ǡଶହΨ ଻଺ǡଶହΨ

  š ͳͲͲΨ ൌ ͻ͵ǡͺͷΨ % Efektivitas Kelompok Ekstrak Dosis 3 =

  ଻ଵǡଶହΨ ଻଺ǡଶହΨ

  š ͳͲͲΨ ൌ ͺͳǡͻ͹Ψ % Efektivitas Kelompok Ekstrak Dosis 2 =

  ଺ଶǡହ଴Ψ ଻଺ǡଶହΨ

  š ͳͲͲΨ ൌ ͳͲͲΨ % Efektivitas Kelompok Ekstrak Dosis 1 =

  ଻଺ǡଶହΨ ଻଺ǡଶହΨ

  % Ef f f ek ek ek k e k ti ti ti i ti vi vi vi vi v ta ta ta a ta a s s s K K K K K K el el e el e om om om ompo po po o po o o o k k k k Ek Ek Ek Ekst st st stra ra ra ra k k k k Do Do Do Do si si si s s s s s 1

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  LAMPIRAN IV Perhitungan Dosis Ekstrak Pada penelitian terdahulu dosis ekstrak etanol 96% daun M. crenata sebesar 2,4 mg/20 g BB mencit.

  Rendemen ekstrak etanol 96% = 19,17% (Laswati, 2007) aswa , 007)

  M M M M. M. M c c c c c re re re re e n n na na na a ta ta ta ta

  Rendemen ekstrak etanol 80% daun M. crenata = 18% endemen n n ek ek ek ek k st st st st st ra ra ra k k k k k k et et e et an an an an ol o ol ol ol ol l

  8

  8 0% 0% 0% 0% 0% d d d d d d d a a au au au n n n n = = = 18% Maka, Maka, dosis pada ekstrak etanol 80% adalah: , do do do do do si si si s s s s s pa pa pa a da da d da da ek ek ek ek ek ek k st st st r k ra ra ra k k k et et et an an an ol ol ol 8 ol

  8

  8

  8 0% 0% a 0% 0% a a ada da da da da da da la la l la la la h h h

  8

  8

  h:

  h:

  h: ͳͻǡͳ͹Ψ ͳͻ ͳͻ ͳͻ ͻ ǡ ǡǡ ͳ͹ ͳ͹ ͳ͹ ͳ͹ Ψ Ψ Ψ Ψ ʹ ʹ ʹǡͶ ʹ ǡ Ͷ Ͷ Ͷ Ͷ

  ൌ ൌ ൌ ൌ ൌ

  ͳͺ ͳ ͳ Ψ ͳ ͳͺΨ Ψ Ψ ݔ ݔ ݔ ݔ ݔ

  ଶǡସ ଶ ଶ ଶ ǡ ǡସ ସ ସ

  ൈ ൈ ൈ ൈ ͳͺ ͳͺ ͳͺ ͺ ͺ ͺ Ψ Ψ Ψ Ψ Ψ Ψ

  = = = = = ൈ ͳͺΨ ଵ ଵଽǡଵ଻Ψ ଵଽ ଵ ǡଵ଻Ψ Ψ Ψ Ψ

  = = = = = 2,25 2, 2, 2, 2, 2,

  25

  25

  2

  25 m m m m mg m g g g g g M M M. M M crenata

  25

  25

  Maka, dosis Maka, dosis 1 untuk ekstrak etanol 80% daun M. crenata

  1

  1

  1

  1 un un un un tu tu tu tu u k ek k k ek ek st s s s s t r r r ra k k k et et et et an an an an n o ol ol ol ol

  8

  8

  8

  8 0% 0% 0% 0% % % d d d d d aau au au au n n n n n n

  adalah sebesar 2,25 mg/20 g BB mencit. dalah sebesar 2,25 m m m g/ g/ g/ g/ /

  20 20 g g g g g B B B B B B B B B m me me me me nc n nc nc nc it. it it

  20

  20 ariable: juml ah ah ah h h ge ge ge g g li li li l i at at at at a

  mparisons ns ns s s s

  71

  30

  30

  3

  3

  3

  3

  3

  33

  71 ,8 ,8 ,8 ,8

  71

  71

  30

  2

  6

  6

  26

  26

  2

  26

  ps s s

  4 ,5

  41

  4

  30

  89

  8

  2

  3

  35

  35

  3

  75 75 7 0

  4 4,75

  15 4, 4,

  15

  15

  15

  1

  89

  1

  1

  2

  2

  21

  1

  1

  6

  61

  61

  89 9,0 ,0 ,0 ,061

  4

  83

  8 48 2, 2, 2 2, 2 ,

  Sum of of o of of Sq Sq Sq q q q ua ua u ua a re re re s s s df df f df df

  48

  48

  48

  8

  18

  18

  18

  18

  Me M Me Me Me an a a a Sq Sq Sq Sq S ua a a a a re re re re re F

  LAMPIRAN V Analisis Statistika Oneway Anova

ANOVA

  83

  Upper Bound kontrol negatif kontrol positif

  Lower Bound

  95% Confidence Interval

  Std. Error Sig.

  Mean Differenc e (I-J)

  (I) kelompok (J) kelompok

  35 Multiple Comparisons Dependent Variable: jumlahgeliat LSD

  2671,833 30 89,061 Total 21154,750

  18482,917 5 3696,583 41,506 ,000 Within Groups

  Between Groups

  48

  9

  jumlahgeliat Sum of

  5

  8

  9 9 ,5 ,5 ,583

  6

  6

  96

  96

  96

  36 3696

  36

  36

  5

  91

  5

  5

  7

  7

  7

  1

  9

  9

  91

  91

  Squares df Mean Square F Sig.

• 5,449 ,000 49,37 71,63 dosis (1) 53,333

  60,500

• 5,449 ,000 42,21 64,46 dosis (2) 56,333
• 5,449 ,000 45,21 67,46

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  kontrol positif kontrol negatif

• 5,449 ,000 -71,63 -49,37 dosis (1) -7,167 5,449 ,198 -18,29 3,96 dosis (2) -4,167 5,449 ,450 -15,29 6,96 dosis (3) ,667 5,449 ,903 -10,46 11,79 dosis (4) 6,667 5,449 ,231 -4,46 17,79 dosis (1) kontrol negatif
• 60,500
• 5,449 ,000 -64,46 -42,21 kontrol positif
• 53
• 53,333
• 5, 5,
• 5,449 ,017 2,71 24,96 dosis (2) kontrol negatif

  7,167 5,449 ,198 -3,96 18,29 dosis (2) 3,000 5,449 ,586 -8,13 14,13 dosis (3) 7,833 5,449 ,161 -3,29 18,96 dosis (4) 13,833

  6

  44

  44 449

  9 9 ,4 ,4 ,4

  4

  4

  4

  4

  50

  50

  50

  50 50 -6 6,

  6

  7 7 5, 5, 5, 5,

  6

  9

  do do do d do do si s si s s s s (1 (1 (1 ( ( ) ) ) ) - - - - 3, 3, 3,

  00

  

00

00 5, 5, 5,

  5

  44

  44

  4

  4

  44

  44

  44

  7

  9

  00 00 - -

  

33

  

33

  33

  44

  44

  44

  49

  9

  9

  9 ,0 ,0 ,0

  00

  67

  7

  67

  67

  67 7 ,4

  4

  4

  4 ko ko ko ko ko nt nt n nt t n ro r ro o o ro o l l ll

  po po po po po si si si i ti ti ti ti t f f f f 4, 4, 4,

  4

  

16

  

16

  6

  16

  9

  9

  56

  

8

  3

  3

  82

  2

  82

  82

  82 82 -6,2

  dosis (4)

  10

  10

  10 10 ,8 ,8 ,8 ,8

  33

  9 9 ,3 ,3 ,3

  

33

  

33

3 5, 5, 5, 5,

  5

  44

  4

  44

  44

  4

  4

  9

  9

  9 9 ,056 -,2

  3

  9

  9

  

83

  9 9 ,5

  5

  5

  5

  86

  6

  6

  6 6 -14,1

  dosi si sis s s s s s (3 (3 (3 (3 (3 ( ) ) ) ) 4, 4, 4,

  4

  3

  

3

  

8

  9

  

83

  

83

  3

  3

  3

  3 3 5, 5, 5 5,

  44

  44

  44

  44

  4

  9

  

6 ,3 ,3 ,3 ,333

  ko ko ko ko k ko n n nt t t n n ro ro ro ro l l l ne ne ne n g ga ga a g a g ti ti t ti f f f f

  4,167 5,449 ,450 -6,96 15,29 dosis (1) -3,000 5,449 ,586 -14,13 8,13 dosis (3) 4,833 5,449 ,382 -6,29 15,96 dosis (4) 10,833 5,449 ,056 -,29 21,96 dosis (3) 7,2 mg kontrol negatif

  9 9 ,1 ,1 1 ,1

  5 5, 5 5, 5,

  44

  44

  44

  44

  4

  4

  4

  9

  9

  9

  1

  7

  98

  98

  9

  9 8 -3,9

  do do do o si si si s s s s s s (2 (2 (2 (2 (2

  2 2) ) ) ) 3, 3, 3, 3,

  3

  00

  

00

  00

  00 5, 5, 5, 5,

  44

  7 5 5,

  7

  44

  5

  kontrol negatif f f

  53

  5

  53 53 ,3 ,3 ,3 ,3 ,3

  

33

  

3

  

3

  

33

  33

  

3

  5, , 5, 5,

  44

  

16

  44

  44

  4

  4

  4

  9

  9

  9 9 ,000 -64,4

  ko o o nt nt nt ro r ro ro ro l l l l

  po po po po p si si si s ti t ti ti t f f f 7, 7 7, 7,

  16

  

16

  44

  44

  7

  4 4 9

  6 1 - - - 3, 3, 3, 3,

  2 do do do d d s si s i i s s s s s (4 (4 (4 ) ) ) )

  13

  13

  13 13,8 ,8 ,

  33

  3

  

3

  5

  5

  44

  9

  1

  9

  9 ,0 ,0 ,0

  17

  17

  17 7 2,

  2

  2

  2

  2

  7

  7

  7

  1

  61

  9

  8

  9

  9

  9 ,5

  5

  5

  5 ,5 5 ,5 586

  86

  86

  6

  6 6 - - - 8,

  8

  8

  1 do do do do d si si si s si s s s s s s (3 (3 (3 3 ) ) ) ) 7, 7, , 7,

  6

  

83

  

83

  83 833

  3

  3

  5 5 5,

  44

  44

  44 449

  9

  9

  9 ,1 ,1 ,1

• 5,449 ,000 -67,46 -45,21 kontrol positif
• 5, 5 5, 5,
• 56
• 56,333
• 5,449 ,000 -72,29 -50,04 kontrol positif
• 61,167
• ,667 5,449 ,903 -11,79 10,46 dosis (1) -7,833 5,449 ,161 -18,96 3,29 dosis (2) -4,833 5,449 ,382 -15,96 6,29

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  dosis (4) 6,000 5,449 ,280 -5,13 17,13 dosis (4) 9,6 mg kontrol negatif

  

Descriptives

• 5,449 ,000 -78,29 -56,04 kontrol positif
• 67,167
• 6,667 5,449 ,231 -17,79 4,46 dosis (1) -13,833
• 5,449 ,017 -24,96 -2,71 dosis (2) -10,833 5,449 ,056 -21,96 ,29 dosis (3) -6,000 5,449 ,280 -17,13 5,13 *. The mean difference is significant at the 0.05 level.

  10

  9 9 ,2 ,2 ,2

  44

  44

  44

  4

  9

  9

  2

  5

  80

  8

  8 8 -17,1

  differen n en e ce ce e ce e i i is s s s si si si i s s gn gn gn if if if i ic i ic ic c an an a an n an a t t t t t t t t at at at t t t t t he he h he he he he h 0.0 .0 .0

  5

  5

  5 5 le le le le l ve ve ve v v l. l. l.

  5

  

00

00 5, 5, 5,

  10

  5

  10 10 ,8 ,8 ,8

  

8

8 ,8

  

33

  

33

  

33

  

33

33 5, 5, 5, 5,

  44

  

00

  44

  4

  4

  4

  9 ,056 -21,9 dosis (3 (3 (3

  3 ( ) ) ) ) -6, 6, 6, 6, ,

  dosis (2) -10

  00

  

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

Statisti Std.

  kelompok c Error jumlahgel kontrol Mean 79,83 8,619 iat negatif

  95% Confidence Lower 57,68

  Interval for Mean Bound Upper

  101,99 Bound Bound

  5% 5% 5% 5% 5% Trimmed Mean 5% % T T T T ri ri ri ri mm mm mm mm m ed ed ed ed e M M M ea ea ea e ea n n n 80,15 80, Me Me Me Median e di d dii di di an a an an an n n n 80,00 80,

  V Va V a ri ri ri an a a a an an an c c ce ce ce ce c 44 445,76

  Variance Va Va

  4 44 5,

  44

  7 Std. Deviation S d. St St St d.

d. D D D evia e ev ia ia ti ti ti ion on on n

  21

  21 21 21,113 1 ,1 ,1 ,1

  21

  2

  1 Minimum Miini Mi Mi n ni imu mum mu m m m

  1

  1

  50 Ma Ma Ma Maximum Ma x xi x xi mu mu m m m m

  1 104

  Range R ng Ra Ra Ra ng ng e e e e

  54 In

  I In In Interquartile Range te te te te rq rq rq u ua ua ua rt rt r rt il i il e e Ra Ra R Ra ng ng ng ng e e e e

  40 Skewness Sk Sk Sk Sk Sk S ew ew ew ew ew ne ne ne e s ss ss ss -,237 -,2 ,845

  K Ku Ku Ku Kurtosis K rt rt rt t t os os os os os is is is is s -1,476 -1,4 1,741

  kontrol kontrol Me Me Me Mean M Me e an an an n 19,33 19, 2,525 positif 95% Confidence Lower

  12,84 Interval for Mean Bound

  Upper 25,83

  Bound 5% Trimmed Mean 19,37 Median 18,50

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  26

  29

  29

  2 9 , ,

  5%

  5

  5

  5 Trimm mm mm mmed ed ed ed M M M M ea ea ea ean n n n

  26

  26

  26

  29

  26 6 , , Me e Me Me di d an an an an

  27

  27

  27 , Va Va Va Varii ri ri an an an ce ce ce ce 6 6,

  6

  7 S S St St S d.

  d.

  d. d d D D D D D D D ev ev ev e e e e ia ia ia ti ti ti on on on 2,5 Mi Mi Mi M M ni ni ni n mu m mu mu u m m m m

  Ma a axi xi xi mu mu mu m mu u m m m m m

  29

  Variance 38,267 Std. Deviation 6,186 Minimum

  10 Maximum

  4 Skewness -1,245 ,845 Kurtosis ,991 1,741 dosis (2) Mean 23,50 ,847

  28 Range

  18 Interquartile Range

  10 Skewness -,096 ,845 Kurtosis ,317 1,741 dosis (1) Mean 26,50 1,057 95% Confidence Interval for Mean

  Lower Bound

  23,78 Upper Bound

  29,22 5% Trimmed Mean 26,61 Median 27,50 Variance 6,700 Std. Deviation 2,588 Minimum

  22 Maximum

  29 Range

  7 Interquartile Range

  Sk Sk k Sk k ew ew ew ew w ne e ne ne ss ss ss ss ss ss -,0 Ku Ku Kurt rt r r os os os os o is is is is is ,3 dosis s (1 (1 ( (1 (1 (1 ) ) ) )) Me Me Me e M a a a an an an 26,

  2 3 , Up Up Up Up pe pe pe per r r

  95

  95

  95

  5 % % % % % % Co Co Co Co Co Co Co onf nf nf nf id id id id en en en en ce ce ce ce

  In In In n te e e e rv rv rv v rv rv a al al al a a al f f f for or or or M M M M ea ea ea ea n n n

  Lo Lo L Lo we we e e we e w e r r r r

  Bo Bo B Bo un un un un d d d d

  23

  23

  23

  Bo Bo Bo B un un un u d d d d

  95% Confidence Interval for Mean

  do o o si si si si i s s s s (3 ( (3 (3 (3 ) ) ) ) Me e Me Me an an a

  2

  5

  5

  5

  5

  5

  18

  2 2,

  18 18 ,

  95

  95

  95 95% % % % Co Co C Co nf nf n nf id d d d en en en ce ce ce e

  In n n n n te te te t e rv rv rv rv rval al al l f f f or or or Me Me Me M an an an n Lo Lo Lo Lo w w we we we e we r r r r Bo Bo Bo B B un un un u un d d d

  14, Up Up Up Up Up pe pe pe pe pe pe r r r r Bo Bo Bo B o B und

  2

  Ku K K K rtosis s s s - 2,

  Lower Bound

  Lower Bound

  21,32 Upper Bound

  25,68 5% Trimmed Mean 23,50 Median 23,50 Variance 4,300 Std. Deviation 2,074 Minimum

  21 Maximum

  26 Range

  5 Interquartile Range

  4 Skewness ,000 ,845 Kurtosis -2,526 1,741 dosis (3) Mean 18,67 1,764 95% Confidence Interval for Mean

  14,13 Upper Bound

  Sk Sk Sk k ew ew ew ne ne ne ness s ,0

  23,20 5% Trimmed Mean 18,69 Median 18,50 Variance 18,667 Std. Deviation 4,320 Minimum

  13 Maximum

  24 Vari ri ri an an an n an ce ce ce ce e c 4,3 St t St St St St d

  d. d

  d. d D D D D ev ev ev v e ia a ia a i ti ti i i i t on o o on n 2,0 M Mi M M ni ni ni n n n mu mu mu m mu mu mu m m m m Ma Ma Ma Ma xi xi xi mu mu mu mu mu mu mu m m m m

  R Ra R R ng ng ng g g n ng e e e e e e e e

  In n In n te te te rq rq rq qua ua u u rt t il il il e e e e Ra R R ng ng ng n e

  23,

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  Range

  11 Interquartile Range

  9 Skewness ,006 ,845 Kurtosis -1,481 1,741 dosis (4) Mean 12,67 1,856 95% Confidence Lower

  7,90 7, Interval for Mean In n n te te te e r rv rv rv v a al al al f f f f f or or or or or r M M M e ea ea ea ea ea n n n n Bo B B Bound B B und

  Up Up Up Upper p p p pe pe pe pe pe r r r r 17, 17,44

  Bound B Bo Bo Bo Bo o un un un un un d d d d d d 5% Trimmed Mean 5% 5% 5% % T T T T T T ri ri ri r r ri mm mm mm m m m m m ed ed ed ed M M M M ea ea ea ea n n n n

  12 12,74 , M d Median Me M M di d di di di di a an an a an an an an

  1

  12 1 12,50 ,

  12

  12

  V ri Va Va Variance Va ri ri an an an n ce c c 20,667

  20

  20

  20 ,6 ,6

  2

  6

  6 St St St Std. Deviation t

  6

  d. d d D D D ev e iation ev ev o o o 4,

  4

  4 4 4,546

  4

  5 M M nimum Minimum M Mi m m m

  5

  5

  6 Ma Ma Ma Maximum a x x xi mu mu mu mum m m m

  18 Ra Ra Range Ra Ra ng ng n ng e e e e

  12 In Interquartile Range In n n n t te te t te rq rq rq rq q q q q ua ua ua ua ua u a rt rt rt il il il e e e Ra Ra Ra ng ng n ng ng ng g e e e e e e e

  8 Sk Sk Sk S S Skewness ew ew ew e w ne ne ne ne ss ss ss -,2 -,270 ,845 Ku Kurtosis rt rt rt os os os os s i is is is s s -1,008 -1,0 1,741

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  

10

  00

  00

  00

  1

  1 1 . . .

  St St St t St t em em em e em em w w w i id d id id i i th th h th : :

  

10

  2, 2, 2,

  10 1 0

  Ea Ea a Ea Ea ch ch ch c ch c l l l l l l ea ea a af:

  f: f

  f:

  f: f 1 ca se (s) ) ) ) )

  umlah h h h h ge ge ge g g li li li li i l at at at a t S S S S S S te te te te te e m m m m- -an an and d d -Le Le Le eaf af a af af a P P P P l lo lo lo t t t t fo fo fo fo o r r r r elompok ok ok= = = = = ko ko ko ko o nt nt nt ro ro ro ro ro o l l l l po po po po p o si si s si i si ti ti ti i f f f f f Frequency S S S S S S te te te te e m m m m m & & L L L L L ea ea ea ea e f f f f f f

  2

  Uji Normalitas dan Homogenitas Stem-and-Leaf Plots

  jumlahgeliat Stem-and-Leaf Plot for kelompok= kontrol negatif Frequency Stem & Leaf ,00 0 . 4,00 0 . 5678 2,00 1 . 00 Stem width: 100 Each leaf: 1 case(s) jumlahgeliat Stem-and-Leaf Plot for kelompok= kontrol positif Frequency Stem & Leaf 1,00 1 . 0 3,00 1 . 789 1,00 2 . 4 1,00 2 . 8 Stem width: 10 Each leaf: 1 case(s)

  5

  elompok= kont nt t t ro ro o ro o l l l l l ne n n ne ne ga ga a a ga ti ti ti ti ti ti f f f f f Frequenc c nc c nc c y y y y S S S S S te te te e e t m m m m & & & & L L L L ea ea ea ea a f f f f f f ,0 ,0 ,0 . . .

  4, 4, 4,

  4 ,

  00

  00 00 . . .

  5

  5

  8

  5

  67

  67

  67

  67

  8

  8 jumlahgeliat Stem-and-Leaf Plot for kelompok= dosis (1) Frequency Stem & Leaf 1,00 2 . 2 5,00 2 . 57889 Stem width: 10 Each leaf: 1 case(s) jumlahgeliat Stem-and-Leaf Plot for kelompok= dosis (2) Frequency Stem & Leaf 3,00 2 . 122 3,00 2 . 556 Stem width: 10 Each leaf: 1 case(s) jumlahgeliat Stem-and-Leaf Plot for kelompok= dosis (3) Frequency Stem & Leaf

  5,00

  5

  1

  1

  22

  22

  22

  22

  3, 3, 3 3, 3, 3,

  00

  00

  00

  2

  2

  

2 . . . 5

  5

  2

2 . .

  56

  56

  56

  56

  6

  6 Stem wid d d d th th th h t : : : :

  1

  1

  1

  

1

  1 Each leaf:

  

1

  1

  1

  2

  2

  89

  2

  2

  2

2 .

  5

  5

  5

  5

  5

  78

  78

  78

  78

  89

  89 Stem wid id id d d d th th th th th t : :

  00

  10

  1

  10

  10 Each l l l ea ea a ea a

  f:

  f:

  f:

  

1

  1

  

1

1 ca ca ca ca se se se s e se s se (s (s (s s (s ) u ml ml ml l l ah ah h h h h ge g g e g g li li li liat at at S S Ste te te tem m m m - - - and d d d -L L Lea f f P P P P lo lo lot t t f fo o o o f r r r r e e e e lo lo lo lo l mp p p p p ok ok k k ok ok = = = do do do osi si si si s (2 (2 (2 2) ) ) )

  Fr r r r eq eq eq eq eq q ue ue ue u ue nc nc nc nc y y y y S te te te tem m m m & & & & L L L Lea ea ea eaf 3, 3, 3,

  3 ,

  00

  00

  1 ca ca ca ca c se se se se s (s (s s (s) ) ) )

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  1,00 1 . 3 3,00 1 . 589 2,00 2 . 34 Stem width: 10 Each leaf: 1 case(s) jumlahgeliat Stem-and-Leaf Plot for kelompok= dosis (4) Frequency Stem & Leaf 1,00 0 . 6 3,00 1 . 014 2,00 1 . 78 Stem width: 10 Each leaf: 1 case(s)

  1

  

1 1 . 7

  7

  7

  8 St St St S t em e e e e e w w w w w id i id d d i d i i d th th th th th th: : : :

  1

  

1

  f:

  1

  1

  f: f

  f:

  1

  

1

  

1

  1 ca ca ca a se se se s (s s (s s) ) ) )

  1

  00

  umlah h h h h ge ge ge g li li li li li l at at at t at S S S S S S te te te te t m m m m m m m -an an an d d

  00

  d- - - Le Le Le Le Le Le Le af af af af P P P P lo lo lo l t t t t t fo fo fo r r r r r

  elomp mp mp p ok ok ok o o = = = do do do d do si si si si si i si s s s (4 (4 (4 (4) ) ) Fr r r r eq eq eq eq ue ue ue u nc nc nc n n c y y y y y S S S S S te te e e e e e m m m & & & & L L L L ea ea ea ea f f f

  1, 1, 1 1,

  1

  00

  00 00 0 . 6 3, 3, 3, , 3,

  00

  00

  00

  1

  1

  1 1 . 0

  14

  4

  4

  2, 2 2, , 2 2,

1 Ea a a a ch ch ch ch h c l l l l l ea ea ea ea

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  LAMPIRAN VI Dokumentasi Gambar 1

  Ekstrak Etanol 80% daun M.

  crenata Gambar 2

  Proses Maserasi

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  Ga Ga Ga Ga G Gambar 3 a mb mb mb mb m b ar a ar ar

   3 Pr Pr P P Proses penyaringan r os os os o es es es s pe pe p pe pe ny ny ny ny ny ar ar ar ar ingan de de de de de ng n n ng ng g an an an an n co co co co co ro ro ro o o ng ng ng ng g bu b b b bu chner

  dengan corong buchner

  Gambar 4

  Proses penguapan ekstrak dengan rotary evaporator

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  Ga Ga G Ga Ga Gambar 5 mb mb mb m m ar 5

  Hewan uji mencit He He He He He H wa wa wa wa n n n n n uj uj uj uj u i i i i mencit

  b ba ba ba baa ba lb l lb lb lb b /c /c /c /c /c j j j j j j an an an n ta ta ta ta a n n n n

  balb/c jantan

  Ga Ga Ga Ga Gambar 6 mb mb m m b ar ar a ar r

   6

  6 Kandang mencit Ka Ka Ka nd nd nd nd a an an an an a g g g g me me me m n nc nc nc Gambar 7

  Timbangan mencit

ADLN-PERPUSTAKAAN UNIVERSITAS AIRLANGGA

  Gambar 8

  Atas : jarum injeksi IP Bawah : sonde oral mencit

  

Ga Ga Ga Gambar 9 Ga mbar mb mb mb ar a ar

   9

   9

   9

  9 P Pr Pros Proses injeksi intraperitonial pada Pr os os e es es es i i i nj nj nj nj ek ek ek ksi si si si in in in tr tr trap ap ap p p p er er er er e e it it it it t t onia on on ia a l l l pa l p pa pa pa da

m me me mencit nc nc nc it it it