Isolation and Molecular identification with 18SRDNA leaf Base Root Desease on Aloe barbadensis in Bali.
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Gonference Programs
r_
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2015 (ICPD)
sth lnternational Conference on Plant Diversity
Taxonomy
11th Gongress on lndonesian Society of Plant
L.
).-,-r,
Theme: plant Diversity: A Global challenge
I-.-
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TU
LIPI
Graha WidYatama
U niversitas Jenderal Soedirman
Thursday' Friday 20'21 August 2015
!
a-
..S'
e
;e.+ "s
-
lnternational Conference On Plant Diverslty 2015
- 14.4s
14.45 - 15.00
14.30
14.45
-
15.00
15.00- 1s.15
Iime (WlB)
javanicum Miq, And 5. Aqueum ( Burm.F. ) . Alston) Based
On Morpholosv Characteristic
Dewi Susan, Aohvllophorales Funei from Enesano lsland
Bambang Heliyanto, Fatkhur Rochman, Abdurrahman,
Collection Of Sugarcane Genetic Resources ln Sarmi Paoua
Dewi Komariah, Alex Hartana, Morphological Variation Of
Kabau (Arhidendron bubalinum) ln Sumatra
Coffea Break
Presenter of Session 2
Sisunandar, Alkhikmah, Teguh Julianto, Capturing Genetic
Diversity For Ex Situ Conservation Of Kopyor Coconut ln
Graha Widvatama 05
Graha Wiciyatama 01
Graha Widyatama 05
Room
15.15
-
15.30
15.L5
-
15.30
Retno kawuri, lsolation And Molecular ldentification With
18s rDNA Leaf Base Rot Desease On Aloe Barbadensis ln
15.30
-
15.45
Sarah Febriani, Characterization and Standarization of
Cashew cultivars (Anacardium occidentale L)
Graha Widyatama 01
15.30
-
15.45
Graha Widyatama 05
15.4s
-
16.00
15.45
-
16.00
Kartini Kramadibrata, Diversity of Arbuscular Fungi in
Batukau Nature Reserve and Mesehe Mountain. Bali
Murni Sapta Sari, Application of Project-Based Strategy on
Algae Diversity Material as an Attempt to lmprove Biology
Teacher Candidates Metacosnitive Skills
Pudji Widodo, Four New Subspecies Of Syzygium
Graha Widyatama
01.
Banyumas Areas
Graha Widyatama 05
Bali
15.00
15.00
-
16.1s
15.15
-
16.30
15.15
-
15.30
16.30
-
15.45
16.ls
Samarangense (Myrtaceae) From lndonesia
Niken Kusumarini, Diversity of Cubeb in Java
Endang Katikowati, Effect Of Combinations Various Plant
Spacing and Number of Seeds on Plant Growth and Rice
Yield Ciherang Varieties
Tatik Chikmawati, Genetic diversity of wild rambutans
(Nephelium) in Sanggau, West Kalimantan based on SSR
and ISSR markers
Merry Antralina, The effect of Bokashi Dosage on the
Growth of Arabica Coffee Seedling (Coffea arabica L.)
Cultivar Andungsari l.
Eko Sri Sulasmi, Spore Characters On Some Genus Of
Graha Widyatama 01
Graha Widyatama 05
Graha Widyatama 01
Graha Widyatama 05
Graha Widyatama 01
Graha Widyatama 05
Graha Widyatama 01
Filicales Allies
16.30
-
16.45
16.45
-
17.00
L6.45
-
L7.OO
17.00
-
17.15
Suratman,Analysis of Genetic Variability in Avocado (Persea
americana Mill.) Population ln Central Java Based on
Morphological and Molecular Characters
Mariana, Feliatra Felix, Sukendi, Syafruddin Nasution,
Diversity of Mangrove Vegetation in Kuala tndragiri Coastal
Area Riau Province - lndoneisa
Titien Ngatinem Praptosuwiryo, The Diversity of Ferns and
Fern Allies in Bogor Botanical Gardens
Nurainas, Preliminary Study of the Genus Zingiber
L7.00
*
77.15
(Zingiberaceae) in Sumatra
Nunik Sri Ariyanti, The genus Bazzania of Mount Sibayak,
North Sumatra
17.t5
-
17.30
Wisnu Handoyo Ardi, Daniel Caspar Thomas, Mark
Graha Widyatama 05
Graha Widyatama 01
Graha Widyatama 05
Graha Widyatama 01
Graha WidVatama 05
Graha Widvatama 01
Hughes, Studies Begonia from Molucca lll: Reinstatement
of two Begonia muricata svnonVms
vi I enrsrrurnloN scHEDULE
Fakultas Biologi I Universitas Jenderal Soedirman
l-
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q:r: rlir * .r
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lnternational Conference On plant Diversity 2015
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lsolation And Morecurar tdentification with 1gs rDNA
Leaf Base Rot Desease
On Aloe Barbadensis tn Bali
E
Retno Kawuri
E
Laboratory Microbiorogy Facurty Mathematic and science
Udayana
Em ail: microbiologylaboratory@yahoo.com
ABSTRACT
b
Aloe vera plants (A.barbadensis Miller) has been planted
in Bali since 2006 and spread out n five
regencies, i'e' Buleleng, Karangasem, Bangli, Gianyar
and Badung; invotving 13g farmers, with
total area 170 hectare. Disease that h'ave oestroyeo
Atoe vera L reif base rol witn the symptom
of rotten base
leaf' The objectives of this researih were to know the occurrence
of pathogen in
Bali and to identify the cauCal agent that responsible
for the leaf base rot disease on atoe. survey
on leaf base rot disease in Aloe vera piants was carrieJ out
in 5 regencies in Bali, i.e.
Karangasem, Buleteng,. Bangli, Gianyar and Badung.
eainogen isolation on sample was
conducted until pure culture was obtained, and then wal
inoculated on healthy Aloe vera plants
to confirm that the same symptom occurred- il tlre
eostulate test). The causal agent
irocrr
1en
was identified by molecular identification of 18s rDNA.
Results showed that the leaf base rot
disease was distributed.in all five regencies in Bali
wiih
causal agent of the leaf base rot disease was Fusaium
b
"r;;rg;incidence
ir4"iZ*^.
was 13.43% and the
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lnternational Conference On plant Diversity 20L5
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Acknowledgements
we have been supported by grant from Rector of Unsoed,
who has given us permission ot use
this building for free' Dean and Vice Deans of
Fakultas Biologi Unsoed are thanked for support.
we also would like to thank Mr wisnu as the donatur. we
would like to thank prof. Mien A.
Rifai' and Prof' Dr' Dedy Darnaedi, who have
trust us to be the committee of the 5th
lnternational conference on Plant Diversity lcPD
2015 at Unsoed purwokerto. prof Ferry slik
from the Herbarium of University Brunei Darussalam,
Dr. Chandrakant Shalunke from Botany
lndia and Dr campbell webb (Harvard University
Herbaria), are acknowledged for their willing
to be the keynote speakers. special thank we due to vijayasankar
Raman phD (Univ. of
Mississippi usA) for critically reviewing some papers.
I wourd like to express my gratitude to
Prof' Dr Estri Laras Arumingtyas for assistance and
advice. we are grateful to Dr Titi
Rugayah
and Mrs Yessi santika MSi who has coordinated
this program from Bogor. we also would tike
to thank all participants who have come to Purwokerto
for your presentation. , ,ro*i"
.,
the committee members of the lcPD 2015 who have
worked hard for the success of this
conference' Finally, we hope that this conference can
increase our awareness on the
importance of prant Diversity for our better future.
Furthermore, we ;;;" ;;a by this
conference we can increase our collaboration
amongst all participants and keynote speakers.
Thank you.
Purwokerto,
August 20,2OlS
Committee
u2
l
.&
r-4
a,--
l_
H
l_
).,..l_
Gonference Programs
r_
)-.-
2015 (ICPD)
sth lnternational Conference on Plant Diversity
Taxonomy
11th Gongress on lndonesian Society of Plant
L.
).-,-r,
Theme: plant Diversity: A Global challenge
I-.-
l_.
r--a
L
--.L
rlr
TU
LIPI
Graha WidYatama
U niversitas Jenderal Soedirman
Thursday' Friday 20'21 August 2015
!
a-
..S'
e
;e.+ "s
-
lnternational Conference On Plant Diverslty 2015
- 14.4s
14.45 - 15.00
14.30
14.45
-
15.00
15.00- 1s.15
Iime (WlB)
javanicum Miq, And 5. Aqueum ( Burm.F. ) . Alston) Based
On Morpholosv Characteristic
Dewi Susan, Aohvllophorales Funei from Enesano lsland
Bambang Heliyanto, Fatkhur Rochman, Abdurrahman,
Collection Of Sugarcane Genetic Resources ln Sarmi Paoua
Dewi Komariah, Alex Hartana, Morphological Variation Of
Kabau (Arhidendron bubalinum) ln Sumatra
Coffea Break
Presenter of Session 2
Sisunandar, Alkhikmah, Teguh Julianto, Capturing Genetic
Diversity For Ex Situ Conservation Of Kopyor Coconut ln
Graha Widvatama 05
Graha Wiciyatama 01
Graha Widyatama 05
Room
15.15
-
15.30
15.L5
-
15.30
Retno kawuri, lsolation And Molecular ldentification With
18s rDNA Leaf Base Rot Desease On Aloe Barbadensis ln
15.30
-
15.45
Sarah Febriani, Characterization and Standarization of
Cashew cultivars (Anacardium occidentale L)
Graha Widyatama 01
15.30
-
15.45
Graha Widyatama 05
15.4s
-
16.00
15.45
-
16.00
Kartini Kramadibrata, Diversity of Arbuscular Fungi in
Batukau Nature Reserve and Mesehe Mountain. Bali
Murni Sapta Sari, Application of Project-Based Strategy on
Algae Diversity Material as an Attempt to lmprove Biology
Teacher Candidates Metacosnitive Skills
Pudji Widodo, Four New Subspecies Of Syzygium
Graha Widyatama
01.
Banyumas Areas
Graha Widyatama 05
Bali
15.00
15.00
-
16.1s
15.15
-
16.30
15.15
-
15.30
16.30
-
15.45
16.ls
Samarangense (Myrtaceae) From lndonesia
Niken Kusumarini, Diversity of Cubeb in Java
Endang Katikowati, Effect Of Combinations Various Plant
Spacing and Number of Seeds on Plant Growth and Rice
Yield Ciherang Varieties
Tatik Chikmawati, Genetic diversity of wild rambutans
(Nephelium) in Sanggau, West Kalimantan based on SSR
and ISSR markers
Merry Antralina, The effect of Bokashi Dosage on the
Growth of Arabica Coffee Seedling (Coffea arabica L.)
Cultivar Andungsari l.
Eko Sri Sulasmi, Spore Characters On Some Genus Of
Graha Widyatama 01
Graha Widyatama 05
Graha Widyatama 01
Graha Widyatama 05
Graha Widyatama 01
Graha Widyatama 05
Graha Widyatama 01
Filicales Allies
16.30
-
16.45
16.45
-
17.00
L6.45
-
L7.OO
17.00
-
17.15
Suratman,Analysis of Genetic Variability in Avocado (Persea
americana Mill.) Population ln Central Java Based on
Morphological and Molecular Characters
Mariana, Feliatra Felix, Sukendi, Syafruddin Nasution,
Diversity of Mangrove Vegetation in Kuala tndragiri Coastal
Area Riau Province - lndoneisa
Titien Ngatinem Praptosuwiryo, The Diversity of Ferns and
Fern Allies in Bogor Botanical Gardens
Nurainas, Preliminary Study of the Genus Zingiber
L7.00
*
77.15
(Zingiberaceae) in Sumatra
Nunik Sri Ariyanti, The genus Bazzania of Mount Sibayak,
North Sumatra
17.t5
-
17.30
Wisnu Handoyo Ardi, Daniel Caspar Thomas, Mark
Graha Widyatama 05
Graha Widyatama 01
Graha Widyatama 05
Graha Widyatama 01
Graha WidVatama 05
Graha Widvatama 01
Hughes, Studies Begonia from Molucca lll: Reinstatement
of two Begonia muricata svnonVms
vi I enrsrrurnloN scHEDULE
Fakultas Biologi I Universitas Jenderal Soedirman
l-
*:;*-{f;*'=r
q:r: rlir * .r
I
ft
,**ii:f":,
lnternational Conference On plant Diversity 2015
l=
I
h
lsolation And Morecurar tdentification with 1gs rDNA
Leaf Base Rot Desease
On Aloe Barbadensis tn Bali
E
Retno Kawuri
E
Laboratory Microbiorogy Facurty Mathematic and science
Udayana
Em ail: microbiologylaboratory@yahoo.com
ABSTRACT
b
Aloe vera plants (A.barbadensis Miller) has been planted
in Bali since 2006 and spread out n five
regencies, i'e' Buleleng, Karangasem, Bangli, Gianyar
and Badung; invotving 13g farmers, with
total area 170 hectare. Disease that h'ave oestroyeo
Atoe vera L reif base rol witn the symptom
of rotten base
leaf' The objectives of this researih were to know the occurrence
of pathogen in
Bali and to identify the cauCal agent that responsible
for the leaf base rot disease on atoe. survey
on leaf base rot disease in Aloe vera piants was carrieJ out
in 5 regencies in Bali, i.e.
Karangasem, Buleteng,. Bangli, Gianyar and Badung.
eainogen isolation on sample was
conducted until pure culture was obtained, and then wal
inoculated on healthy Aloe vera plants
to confirm that the same symptom occurred- il tlre
eostulate test). The causal agent
irocrr
1en
was identified by molecular identification of 18s rDNA.
Results showed that the leaf base rot
disease was distributed.in all five regencies in Bali
wiih
causal agent of the leaf base rot disease was Fusaium
b
"r;;rg;incidence
ir4"iZ*^.
was 13.43% and the
b
.a
l-
taE
G
b
G
E'-
G
E-
)
H
)
F-i
I
)
E-r
)
bJ
-J
-J
:-J
FrJ
)
E+
J
ts-a
)
Fif
)
l-r
:J
I-r
-J
tJ
-J
lJ
-I
:J
tr
ao
I easrnacr
t)
g
-l
&ar
,:!rr# :iL g
!6;t'.:at:9
s;1r::ie::
r.#@b,
lnternational Conference On plant Diversity 20L5
fl *W&
ts4
Acknowledgements
we have been supported by grant from Rector of Unsoed,
who has given us permission ot use
this building for free' Dean and Vice Deans of
Fakultas Biologi Unsoed are thanked for support.
we also would like to thank Mr wisnu as the donatur. we
would like to thank prof. Mien A.
Rifai' and Prof' Dr' Dedy Darnaedi, who have
trust us to be the committee of the 5th
lnternational conference on Plant Diversity lcPD
2015 at Unsoed purwokerto. prof Ferry slik
from the Herbarium of University Brunei Darussalam,
Dr. Chandrakant Shalunke from Botany
lndia and Dr campbell webb (Harvard University
Herbaria), are acknowledged for their willing
to be the keynote speakers. special thank we due to vijayasankar
Raman phD (Univ. of
Mississippi usA) for critically reviewing some papers.
I wourd like to express my gratitude to
Prof' Dr Estri Laras Arumingtyas for assistance and
advice. we are grateful to Dr Titi
Rugayah
and Mrs Yessi santika MSi who has coordinated
this program from Bogor. we also would tike
to thank all participants who have come to Purwokerto
for your presentation. , ,ro*i"
.,
the committee members of the lcPD 2015 who have
worked hard for the success of this
conference' Finally, we hope that this conference can
increase our awareness on the
importance of prant Diversity for our better future.
Furthermore, we ;;;" ;;a by this
conference we can increase our collaboration
amongst all participants and keynote speakers.
Thank you.
Purwokerto,
August 20,2OlS
Committee
u2
l
.&