Sampel Berwarna Biru Sampel Bening
Lampiran A. Peta Lokasi Keterangan:
Stasiun 1 : Daerah Perkebunan Stasiun 2 : Daerah Pariwisata Stasiun 3 : Daerah Hilir Sungai
Lampiran B. Bagan Kerja Metode Winkler untuk Mengukur DO Sampel Air
1 ml MnSO
4
1 ml KOHKI Dikocok Didiamkan
Sampel Endapan
Puith/Cokelat1 ml H SO
2
4 Dikocok
Didiamkan
Larutan Sampel
Berwarna Cokelat
Diambil 100 ml Dititrasi Na
2 S
2 O 3 0,00125 N
Sampel Berwarna
Kuning PucatDitambah 5 tetes Amilum
Sampel
Berwarna Biru
Dititrasi dengan Na2S203 0,00125N
Sampel Bening
Dihitung volume Na
2 S
2 O
3 Yang terpakai
Hasil (Suin, 2002)
Lampiran C. Bagan Kerja Metode Winkler untuk Mengukur BOD
5
(Suin, 2002)
diinkubasi selama 5 hari pada temperatur 20°C diinkubasi selama 5 hari pada temperatur 20°C dihitung nilai DO awal
Sampel Air Sampel Air DO Akhir DO Awal
Keterangan :
Penghitungan nilai DO awal dan DO akhir sama
dengan penghitungan Nilai DO
Nilai BOD = Nilai awal – Nilai DO akhir
Lampiran D. Tabel Kelarutan O
2 (Oksigen)
T˚C 0,0 0,1 0,2 0,3 0,4 0,5 0,6 0,7 0,8 0,9
1 13,77 13,74 13,70 13,66 13,63 13,59 13,55 13,51 13,48 13,44
2 13,40 13,37 13,33 13,30 13,26 13,22 13,19 13,15 13,12 13,08
17 9,37 9,35 9,33 9,31 9,30 9,28 9,26 9,24 9,22 9,20
18 9,18 9,18 9,15 9,13 9,12 9,10 9,08 9,06 9,04 9,03
19 9,01 8,99 8,98 8,96 8,94 8,93 8,91 8,89 8,88 8,86
(Barus, 2004)
24 8,25 8,23 8,22 8,21 8,19 8,18 8,17 8,15 8,14 8,13
25 8,11 8,10 8,09 8,07 8,06 8,05 8,04 8,02 8,01 8,00
26 7,99 7,97 7,96 7,95 7,94 7,92 7,91 7,90 7,89 7,88
27 7,86 7,85 7,84 7,83 7,82 7,81 7,79 7,78 7,77 7,76
28 7,75 7,74 7,72 7,71 7,70 7,69 7,68 7,67 7,66 7,65
29 7,64 7,62 7,61 7,60 7,59 7,58 7,57 7,56 7,55 7,54
30 7,53 7,52 7,51 7,50 7,48 7,47 7,46 7,45 7,44 7,43
8,38 8,37 8,36 8,34 8,33 8,32 8,30 8,29 8,27 8,26
23
21 8,68 8,67 8,65 8,64 8,62 8,61 8,59 8,58 8,56 8,55
22 8,53 8,52 8,50 8,49 8,47 8,46 8,44 8,43 8,41 8,40
8,84 8,83 8,81 8,79 8,78 8,76 8,75 58,73 8,71 8,70
20
9,56 9,54 9,52 9,50 9,48 9,46 9,45 9,43 9,41 9,39
3
14,6 14,12 14,08 14,04 14,00 13,97 13,93 13,89 13,85 13,81
14 9,98 9,95 9,93 9,91 9,89 9,87 9,85 9,83 9,81 9,78
15 9,76 9,74 9,72 9,70 9,68 9,66 9,64 9,62 9,60 9,58
10,20 10,17 10,15 10,13 10,11 10,09 10,06 10,04 10,02 10,00
13
10 10,92 10,90 10,87 10,85 10,82 10,80 10,77 10,75 10,72 10,70
11 10,67 10,65 10,62 10,60 10,57 10,55 10,53 10,50 10,48 10,45
12 10,43 10,40 10,38 10,36 10,34 10,31 10,29 10,27 10,24 10,22
4 12,70 12,67 12,64 12,60 12,57 12,54 12,51 12,47 12,44 12,41
5 12,37 12,34 12,31 12,28 12,25 12,22 12,18 12,15 12,12 12,09
6 12,06 12,03 12,00 11,97 11,94 11,91 11,88 11,85 11,82 11,79
7 11,76 11,73 11,70 11,67 11,64 11,61 11,58 11,55 11,52 11,50
8 11,47 11,44 11,41 11,38 11,36 11,33 11,30 11,27 11,25 11,22
9 11,19 11,16 11,14 11,11 11,08 11,06 11,03 11,00 10,98 10,95
13,05 13,01 12,98 12,94 12,91 12,87 12,84 12,81 12,77 12,74
16
Lampiran E. Bagan Kerja Pengukuran Nitrat (NO 3 ) 5 ml sampel air
1 ml NaCl (pipet volum) 5 ml H
2 SO
4
4 tetes Brucine Sulfat Sulfanic Acid
Larutan
Dipanaskan selama 25 menit
Larutan
Didinginkan Diukur dengan
Spektrofotometer pada λ = 410 nm
Hasil
Lampiran F. Bagan Kerja Pengukuran Posfat (PO
4 3- ) 5 ml sampel air
1 ml Amstrong Reagent 1 ml Ascorbic Acid
Larutan
Dibiarkan selama 20 menit Diukur dengan
Hasil
Spektrofotometer pada λ = 880 nm
Lampiran G. Data Mentah Plankton Stasiun 1 Stasiun 2 Stasiun 3
No Takson U1 U2 U3 U1 U2 U3 U1 U2 U3
FITOPLANKTONI Bacillariophyceae A Coscinodiscaceae
1.Coscinodiscus
2
1 2 - - - - - - - - B Diatomaceae .
2.Diatoma 10 - 6 - - - - - -
C Eunotiaceace
3. Eunotia
3
2 - - - - - 1 -
D Fragillariaceae
4. Asterionella
3
2 - - -
1
1 3 -
5.Fragillaria
2 1 - - - - - -
2 E Gomphonemaceae
6. Gomphonema
1 2 - - - - -
2
1 F Naviculaceae
7. Pinnularia
- 2 -
- 3 - - 1 -
8.Navicula
3
8
1
2
2 - G Surirellaceae
9.Cymatopleura -
- 2
2 -
3 - - -
10. Surirella
3
2 - 1 - - - - -
II Chlorophyceae H Oocystaceae
11. Ankistrodesmus
1 2 - - - - - - -
12. Tetraedron
1 - - - - - -
2
1 I Zygnemataceae
13. Spyrogira
17
5
33
25
9 - -
17
10 III Cyanophyceae J
Rivulariaceae
14. Calothrix
2
1 - - - - - 4 - K Stephanodiscaceae
15. Cyclotella
2
1
1 - - - - - -
IV Dinophyceae L Peridineaceae
16. Peridinium
1
1 - - - - - - -
V Euglenophyceae M Euglenaceae
17. Phacus
1 - - - - - - -
1
ZOOPLANKTON
VI Centrohelea N Raphidiophryidae
18. Raphidiophrys 1 -
VII Ciliophora O Spathiidae
19. Spathiodides
2 1 - - - - - - -
VIII Copepoda P Calanoida
20.Nauplius
2
1 - - - - - -
1 IX Cladocera Q Daphnidae
21.Daphnia
1
X Crustaceae R Cyclopidae
22. Diacyclops
2 1 -
23. Megacyclops
1 - 1 - - - - - - S Diaptomidae
24. Diaptomus 1 - - - - - - - -
XI Monogonanta T Brachionidae
25. Brachionus
- 1 - - - - -
26. Keratella
2
Lampiran H. Foto Beberapa Plankton yang Diperoleh Pada Stasiun Penelitian
I. Beberapa Jenis Fitoplankton
Pinnularia Spirogyra Fragilaria Surirella
Navicula Asterionella
Diatoma Peridinium Coscinodiscus Gomphonema.
Cymatopleura Eunotia
Keratella Diacyclops
Beberapa Jenis Zooplankton Diaptomus
Lampiran I. Contoh Perhitungan Plankton Plankton Coscinodiscus pada stasiun 1
1. Kelimpahan Plankton (K)
P/V K Ind/L
0,0196 W = ind/L K 122,45 Ind/L
Coscinodiscus =
2. Kelimpahan Relatif (KR)
KR = x 100% KR = x 100%
Coscinodiscus
KR Coscinodiscus = 2,59%
3. Frekuensi Kumulatif (FK)
100%
FK = x
= 100%
FK Coscinodiscus x FK Coscinodiscus = 66,66%
4. Indeks Keanekaragaman pi ln pi
H’ =
H’ = - {(3/102 ln 3/102) + ( 10/102 ln 10/102) + (3/102 ln 3/102) + (5/102 ln 5/102) + ((3/102 ln 3/102) + ( 3/102 ln 3/102) + (2/102 ln 2/102) + (12/102 ln 12/102) + (2/102 ln 2/102) + (3/102 ln 3/102) + (1/102 ln 1/102) + (55/102 ln 55/102) + ( 2/102 ln 2/102) + ( 2/102 ln 2/102) + (1/102 ln 1/102) + (1/102 ln 1/102) + ( 2/102 ln 2/102) + ( 2/102 ln 2/102) + (1/102 ln 1/102) + ( 2/102 ln 2/102) + (1/102 ln 1/102)
H’ = -(-2,25) H’ = 2,25
5. Indeks Keseragaman
E =
max '
H H
E = E = E = 0,74
6. Indeks Similaritas
IS =
100%
X b a 2c
IS = X 100%
IS= X 100%
IS = 0.81818
IS =81.818 %
51
3
3
3
3 kadar_phosfat Pearson Correlation -.584 .984 -.703 -.686 .565 -.907 .711 .956 1 -.179 -.997
- * Sig. (2-tailed) .603 .116 .503 .519 .618 .277 .497 .189 .885 .048 N
3
3
3
3
3
3
3
3
3
3 kadar_nitrat Pearson Correlation -.796 .888 -.880 -.868 .299 -.990 .474 1 .956 -.458 -.932 Sig. (2-tailed) .414 .304 .315 .330 .806 .089 .685 .189 .697 .236 N
3
3
3
3
3
3
3
3
3
3
3
3 kecepatan_aru s Pearson Correlation .156 .826 .000 .025 .982 -.348 1 .474 .711 .565 -.761 Sig. (2-tailed) .901 .381 1.000 .984 .121 .774 .685 .497 .618 .449 N
3
3
3
3
3
3
3 *. Correlation is significant at the 0.05 level (2-tailed).
3
3
3
3
3
3
3
3
3
3
1 Sig. (2-tailed) .650 .068 .551 .567 .570 .325 .449 .236 .048 .933 N
3 H Pearson Correlation .522 -.994 .648 .629 -.625 .873 -.761 -.932 -.997
- * .105
3
3
3
3
3
3
3
3
3
3
3 kejenuhan_oks igen Pearson Correlation .903 .002 .825 .839 .711 .577 .565 -.458 -.179 1 .105 Sig. (2-tailed) .283 .999 .382 .366 .497 .608 .618 .697 .885 .933 N
3
3
3
3
3
3
Lampiran J. Data Hasil Korelasi Sistem Komputerisasi SPSS Ver.16.00 Correlations DO BOD PH_air Intensitas_cahaya suhu penetrasi_cahaya kecepatan_arus kadar_nitrat kadar_phosfat kejenuhan_oksigen H DO Pearson Correlation 1 -.428 .988 .991 .339 .872 .156 -.796 -.584 .903 .522 Sig. (2-tailed) .718 .099 .084 .780 .325 .901 .414 .603 .283 .650 N
3
3
3
3
3
3
3 PH_air Pearson Correlation .988 -.564 1 1.000
- * .189 .938 .000 -.880 -.703 .825 .648 Sig. (2-tailed) .099 .619 .016 .879 .226 1.000 .315 .503 .382 .551 N
3
3
3
3
3
3
3
3
3
3
3 BOD Pearson Correlation -.428 1 -.564 -.543 .705 -.816 .826 .888 .984 .002 -.994 Sig. (2-tailed) .718 .619 .634 .502 .393 .381 .304 .116 .999 .068 N
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3 penetrasi_caha ya Pearson Correlation .872 -.816 .938 .929 -.164 1 -.348 -.990 -.907 .577 .873 Sig. (2-tailed) .325 .393 .226 .242 .895 .774 .089 .277 .608 .325 N
3
3
3
3
3
3
3
3
3
3
3 suhu Pearson Correlation .339 .705 .189 .213 1 -.164 .982 .299 .565 .711 -.625 Sig. (2-tailed) .780 .502 .879 .863 .895 .121 .806 .618 .497 .570 N
3
3
3
3
3
3
3
3
3
3
3 Intensitas_cah aya Pearson Correlation .991 -.543 1.000
- * 1 .213 .929 .025 -.868 -.686 .839 .629 Sig. (2-tailed) .084 .634 .016 .863 .242 .984 .330 .519 .366 .567 N
3
Universitas Sumatera Utara