Uji In Vitro Aktivitas Antelmintik Ekstrak Etanol Daun Pugun Tanoh [Curanga fel-terrae (Lour.) Merr.]
Lampiran 1. Hasil identifikasi tumbuhan
55
Universitas Sumatera Utara
Lampiran 2. Hasil identifikasi hewan
56
Universitas Sumatera Utara
Lampiran 3. Surat albendazole
57
Universitas Sumatera Utara
Lampiran 3. (Lanjutan)
Sertifikat analisis albendazole
58
Universitas Sumatera Utara
Lampiran 4. Gambar tanaman dan daun pugun tanoh [Curanga fel-terrae
(Lour.) Merr.]
Tanaman pugun tanoh
Daun pugun tanoh
59
Universitas Sumatera Utara
Lampiran 5. Gambar simplisia daun pugun aanoh dan serbuk simplisia daun
pugun tanoh
Simplisia daun pugun tanoh
Serbuk simplisia daun pugun tanoh
60
Universitas Sumatera Utara
Lampiran 6. Hasil pemeriksaan mikroskopik daun pugun tanoh
a
b
a
c
d
e
f
g
h
i
Keterangan:
a. Kristal kalsium oksalat bentuk prisma
b. Tulang daun
c. Rambut berkelenjar
d. Pembuluh angkut bentuk spiral
e. Stomata tipe anomositik
f.lStomata tipe diasitik
g. Sel tetangga
h. Epidermis bergaris
i. Trikoma
61
Universitas Sumatera Utara
Lampiran 7. Gambar seperangkat alat sokslet
Keterangan : 1: Air keluar, 2: Selang air keluar, 3: Pendingin bola, 4: Air masuk,
5: Statif, 6: Siphon sokslet, 7: Serbuk simplisia yang dibungkus
dengan kertas saring dan diikat dengan benang, 8: Selang air masuk,
9: Ember berisi air dan pompa air, 10: Labu alas bulat 500 ml, 11:
Pelarut dan batu didih, 12: Heating mantle, 13: Cok listrik, 14: Klem.
62
Universitas Sumatera Utara
Lampiran 8. Ekstrak etanol daun pugun tanoh
Lampiran 9. Pheretima posthuma
63
Universitas Sumatera Utara
Lampiran 10. Uji pengaruh etanol terhadap Pheretima posthuma
Cacing Pheretima posthuma
Tanpa perlakuan
4 % Mati
0,5 % Hidup
1% Hidup
6 % Mati
8% Mati
2% Mati
10% Mati
64
Universitas Sumatera Utara
Lampiran 11. Uji aktivitas antelmintik terhadap Pheretima posthuma
1
2
3
4
5
6
7
Keterangan : 1: dalam salin (cacing hidup), 2: dalam etanol 0,5 % (cacing hidup),
3: dalam EEDPT 5 mg/ml (cacing mati), 4: dalam EEDPT 10
mg/ml (cacing mati), 5: dalam EEDPT 20 mg/ml (cacing mati), 6:
dalam EEDPT 30 mg/ml (cacing mati), 7: Albendazole 20 mg/ml
(cacing mati).
65
Universitas Sumatera Utara
Lampiran 12. Larutan uji aktivitas antelmintik terhadap Pheretima posthuma
Keterangan: EEDPT 5 mg/ml, 10 mg/ml, 20 mg/ml, dan 30 mg/ml, larutan salin
larutan etanol 0,5 %, suspensi albendazole 20 mg/ml.
66
Universitas Sumatera Utara
67
Universitas Sumatera Utara
Lampiran 14. Perhitungan kadar sari larut air simplisia daun pugun tanoh
x 100%
% Kadar sari larut dalam air =
No.
1.
2.
3.
Berat sampel (g)
5,026
5,046
5,078
Berat sari (g)
0,173
0,175
0,164
a. % Kadar sari
=
0,173 100
x 100% 17,21%
x
20
5,026
b.% Kadar sari
=
0,175 100
x
x 100% 17,34%
5,046
20
c.% Kadar sari
=
0,164 100
x
x 100% 16,15%
5,078
20
% Rata-rata kadar sari larut dalam air =
17,21% 17,34% 16,15%
16,90%
3
68
Universitas Sumatera Utara
Lampiran 15. Perhitungan kadar sari larut etanol simplisia daun pugun tanoh
x
% Kadar sari larut dalam etanol =
No.
1.
2.
3.
Berat sampel (g)
5,011
5,018
5,008
100
20
x 100%
Berat sari (g)
0,272
0,213
0,209
a. % Kadar sari
=
0,227 100
x 100% 22,65%
x
20
5,011
b. % Kadar sari
=
0,213 100
x
x 100% 21,22%
5,018
20
c. % Kadar sari
=
0,209 100
x
x 100% 20,87%
5,008
20
%Rata-rata kadar sari larut dalam etanol =
22,65% 21,22% 20,87%
21,58%
3
69
Universitas Sumatera Utara
Lampiran 16. Perhitungan kadar abu total simplisia daun pugun tanoh
x 100%
% Kadar abu total =
No.
1.
2.
3.
Berat sampel (g)
2,0011
2,0004
2,0057
Berat abu (g)
0,0498
0,0547
0,0586
a. % Kadar abu total
=
0,0498
x 100% 2,49%
2,0011
b. % Kadar abu total
=
0,0547
x 100% 2,73%
2,0004
c. % Kadar abu total
=
0,0586
x 100% 2,92%
2,0057
% Rata-rata kadar abu total
=
= 2,71%
70
Universitas Sumatera Utara
Lampiran 17. Perhitungan kadar abu tidak larut asam simplisia daun pugun tanoh
x 100%
% Kadar abu total =
No.
1.
2.
3.
a.
Berat sampel (g)
2,0011
2,0004
2,0057
% Kadar abu total
Berat abu (g)
0,0096
0,0089
0,0077
=
0,0096
x 100% 0,48%
2,0011
b.
% Kadar abu total =
0,0089
x 100% 0,44%
2,0004
c.
% Kadar abu total =
0,0077
x 100% 0,38%
2,0057
% Rata-rata kadar abu total =
0,48% 0,44% 0,38%
0,43%
3
71
Universitas Sumatera Utara
Lampiran 18. Perhitungan kadar air EEDPT
% Kadar air ekstrak =
No.
1.
2.
3.
Berat sampel (g)
5,007
5,019
5,015
x 100%
Volume awal (ml)
1,20
1,40
1,60
% Kadar air
x 100%
=
a.% Kadar air
=
b.% Kadar air
=
c.% Kadar air
=
% Rata-rata kadar air
Volume akhir (ml)
1,40
1,60
1,80
=
1,40 - 1,20
x 100% 3,99 %
5,007
1,60 1,40
x 100% 3,98%
5,019
1,80 - 1,60
x 100% 3,99%
5,015
3,99% 3,98% 3,99%
3,99%
3
72
Universitas Sumatera Utara
Lampiran 19. Perhitungan kadar sari larut air EEDPT
x 100%
% Kadar sari larut dalam air =
No.
1.
2.
3.
Berat sampel (g)
5,007
5,016
5,011
Berat sari (g)
0,584
0,591
0,589
a.% Kadar sari
=
0,584 100
x
x 100% 58,32%
5,007
20
b.% Kadar sari
=
0,591 100
x 100% 58,91%
x
20
5,016
c.% Kadar sari
=
0,589 100
x 100% 58,77%
x
20
5,011
% Rata-rata kadar sari larut dalam air
=
58,32% 58,91% 58,77%
58,67%
3
73
Universitas Sumatera Utara
Lampiran 20. Perhitungan kadar sari larut etanol EEDPT
% Kadar sari larut dalam etanol =
No.
1.
2.
3.
Berat sampel (g)
5,025
5,012
5,030
x 100%
Berat sari (g)
0,718
0,709
0,712
a.
% Kadar sari
=
0,718 100
x
x 100% 71,44%
5,025
20
b.
% Kadar sari
=
0,709 100
x 100% 70,73%
x
20
5,012
c.
% Kadar sari
=
0,712 100
x 100% 70,77%
x
20
5,030
%Rata-rata kadar sari larut dalam etanol =
71,44% 70,73% 70,77%
70,98%
3
74
Universitas Sumatera Utara
Lampiran 21. Perhitungan kadar abu total EEDPT
x 100%
% Kadar abu total =
No.
1.
2.
3.
Berat sampel (g)
2,0050
2,0032
1,9979
Berat abu (g)
0,0384
0,0366
0,0313
0,0384
x 100% 1,91%
2,0050
a.
% Kadar abu total
=
b.
% Kadar abu total
=
0,0366
x 100% 1,83%
2,0032
c.
% Kadar abu total
=
0,0313
x 100% 1,57%
1,9979
% Rata-rata kadar abu total
=
= 1,77%
75
Universitas Sumatera Utara
Lampiran 22. Perhitungan kadar abu tidak larut asam EEDPT
x 100%
% Kadar abu total =
No.
1.
2.
3.
Berat sampel (g)
2,0050
2,0032
1,9979
Berat abu (g)
0,0070
0,0052
0,0074
a. % Kadar abu total
=
0,0070
x 100% 0,35%
2,0050
b. % Kadar abu total
=
0,0052
x 100% 0,25%
2,0032
c. % Kadar abu total
=
0,0074
x 100% 0,37%
1,9979
% Rata-rata kadar abu total
=
0,35% 0,25% 0,37%
0,32%
3
76
Universitas Sumatera Utara
Lampiran 23. Perhitungan pengenceran etanol
Pengenceran etanol 96% menjadi etanol 0,5%, 1%, 2%, 4%, 6%, 8% dan 10%
dalam 20 ml.
V1.N1=V2.N2
V2.N2
V1=
N1
20 ml x 0,5%
- Etanol 0,5 % =
0,1 ml
96%
0,1 ml etanol 96% diencerkan dengan 19,9 ml aquadest
20 ml x 1%
0,2 ml
96%
0,2 ml etanol 96% diencerkan dengan 19,8 ml aquadest
-Etanol 1 % =
20 ml x 2%
0,4 ml
96%
0,4 ml etanol 96% diencerkan dengan 19,6 ml aquadest
-Etanol 2% =
20 ml x 4%
0,8 ml
96%
0,8 ml etanol 96% diencerkan dengan 19,2 ml aquadest
20 ml x 6%
-Etanol 6 % =
1,25 ml
96%
1,25 ml etanol 96% diencerkan dengan 18,75 ml aquadest
20 ml x 8%
-Etanol 8% =
1,6 ml
96%
-Etanol 4 % =
1,6 ml etanol 96% diencerkan dengan 18,4 ml aquadest
20 ml x 10%
-Etanol 10% =
2 ml
96%
2 ml etanol 96% diencerkan dengan 18 ml aquadest
77
Universitas Sumatera Utara
Lampiran 24. Uji aktivitas antelmintik EEDPT
Waktu Paralisis Pheretima posthuma
Waktu paralisis uji aktivitas
Konsentrasi
Rata-rata
antelmintik (menit)
(mg/ml)
(menit)
I
II
II
Ekstrak 5
162
158
152
157,33
Ekstrak 10
88
95
82
88,33
Ekstrak 20
45
49
48
47,33
Ekstrak 30
50
47
51
49,33
Albendazole
74
78
80
77,33
20
Konsentrasi
(mg/ml)
Ekstrak 5
Ekstrak 10
Ekstrak 20
Ekstrak 30
Albendazole 20
Waktu kematian Pheretima posthuma
Waktu kematian uji aktivitas
Rata-rata
antelmintik (menit)
(menit)
I
II
II
238
245
232
238,33
160
162
152
158
82
92
86
86,67
56
51
58
55
215
204
196
205
SD
5,033
6,506
2,082
2,082
3,005
SD
6,506
5,292
5,033
3,605
9,539
78
Universitas Sumatera Utara
Lampiran 25. Perhitungan penyiapan ekstrak
Perhitungan konsentrasi ekstrak kental daun pugun tanoh dalam 20 ml:
-
EEDPT 5 mg/ml = 5 mg/ml x 20 ml = 100 mg
-
EEDPT 10 mg/ml = 10 mg/ml x 20 ml = 200 mg
-
EEDPT 20 mg/ml = 20 mg/ml x 20 ml = 400 mg
-
EEDPT 30 mg/ml = 30 mg/ml x 20 ml = 600 mg
-
Albendazole 20 mg/ml = 20 mg/ml x 20 ml = 400 mg
-
0,5 Tween 80 = 0,5 % x 20 ml = 0,1 ml
Keterangan : EEDPT = Ekstrak Etanol Daun Pugun Tanoh
79
Universitas Sumatera Utara
Lampiran 26. Data statistik paralisis Pheretima posthuma
Ringkasan Jumlah Sampel
Cases
Valid
Konsentrasi
N
Missing
Percent
N
Total
Percent
N
Percent
waktu_paralisis_uji_akti
ekstrak 5 mg/ml
3
100,0% 0
0,0%
3
100,0%
vitas_antelmintik
ekstrak 10 mg/ml
3
100,0% 0
0,0%
3
100,0%
ekstrak 20 mg/ml
3
100,0% 0
0,0%
3
100,0%
ekstrak 30 mg/ml
3
100,0% 0
0,0%
3
100,0%
albendazole 20 mg/ml
3
100,0% 0
0,0%
3
100,0%
Deskriptif
Std.
Konsentrasi
waktu_paralisis_uji_a
ekstrak 5 mg/ml
ktivitas_antelmintik
Mean
95% Confidence
Interval for Mean
Statistic
157,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
169,8366
158,0000
Variance
25,333
Std. Deviation
5,03322
Minimum
152,00
Maximum
162,00
Range
10,00
Interquartile Range
ekstrak 10 mg/ml
144,8301
.
Median
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
.
-,586
.
88,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
104,4961
.
88,0000
Variance
42,333
Std. Deviation
6,50641
Minimum
82,00
Maximum
95,00
Range
13,00
Skewness
Kurtosis
1,225
.
3,75648
72,1705
Median
Interquartile Range
Error
2,90593
.
,230
.
1,225
.
80
Universitas Sumatera Utara
ekstrak 20 mg/ml
Mean
95% Confidence
Interval for Mean
47,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
Median
.
2,08167
Minimum
45,00
Maximum
49,00
Range
4,00
Interquartile Range
.
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
-1,293
.
49,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
Median
44,1622
54,5045
.
4,333
Std. Deviation
2,08167
Minimum
47,00
Maximum
51,00
Range
4,00
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
.
-1,293
.
77,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
84,9225
.
78,0000
9,333
3,05505
74,00
Maximum
80,00
Range
6,00
Skewness
Kurtosis
1,225
.
1,76383
69,7442
Minimum
Interquartile Range
1,225
.
1,20185
50,0000
Variance
mg/ml
52,5045
4,333
Std. Deviation
albendazole 20
42,1622
48,0000
Variance
ekstrak 30 mg/ml
1,20185
.
-,935
.
1,225
.
Uji Normalitas
81
Universitas Sumatera Utara
Konsentrasi
Kolmogorova
Smirnov
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
waktu_paralisis_uji_a
ekstrak 5 mg/ml
,219
3
.
,987
3
,780
ktivitas_antelmintik
ekstrak 10 mg/ml
,187
3
.
,998
3
,915
ekstrak 20 mg/ml
,292
3
.
,923
3
,463
ekstrak 30 mg/ml
,292
3
.
,923
3
,463
albendazole 20 mg/ml
,253
3
.
,964
3
,637
a. Lilliefors Significance Correction
Deskriptif
95% Confidence
Interval for Mean
Std.
N
Mean
ekstrak 5 mg/ml
3
ekstrak 10 mg/ml
Lower
Upper
Bound
Bound
Min
Max
Deviation
Std. Error
157,3333
5,03322
2,90593
144,8301
169,8366
152,00
162,00
3
88,3333
6,50641
3,75648
72,1705
104,4961
82,00
95,00
ekstrak 20 mg/ml
3
47,3333
2,08167
1,20185
42,1622
52,5045
45,00
49,00
ekstrak 30 mg/ml
3
49,3333
2,08167
1,20185
44,1622
54,5045
47,00
51,00
3
77,3333
3,05505
1,76383
69,7442
84,9225
74,00
80,00
83,9333
41,51844
10,72002
60,9412
106,9255
45,00
162,00
albendazole 20
mg/ml
Total
1
5
ANOVA
Sum of
Squares
Uji Homogenitas
Mean
df
Square
F
Sig.
Levene
Statistic
Between
Groups
Within Groups
Total
23961,600
4
5990,400
171,333
10
17,133
24132,933
14
349,634
,000
1,107
df1
df2
4
10
Sig.
,405
Perbandingan
Tukey HSD
(I)
Konsentrasi
(J) Konsentrasi
Mean
Difference
(I-J)
Std. Error
Sig.
95% Confidence
Interval
Lower
Upper
Bound
Bound
82
Universitas Sumatera Utara
ekstrak 5
mg/ml
ekstrak 10
mg/ml
ekstrak 20
mg/ml
ekstrak 30
mg/ml
albendazole
20 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
*
69,00000
*
110,00000
*
108,00000
*
80,00000
*
-69,00000
*
41,00000
*
39,00000
11,00000
*
-110,00000
*
-41,00000
-2,00000
*
-30,00000
*
-108,00000
*
-39,00000
2,00000
*
-28,00000
*
-80,00000
-11,00000
*
30,00000
*
28,00000
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
,000
57,8772
,000
98,8772
,000
96,8772
,000
68,8772
,000
-80,1228
,000
29,8772
,000
27,8772
,053
-,1228
,000 -121,1228
,000
-52,1228
,973
-13,1228
,000
-41,1228
,000 -119,1228
,000
-50,1228
,973
-9,1228
,000
-39,1228
,000
-91,1228
,053
-22,1228
,000
18,8772
,000
16,8772
80,1228
121,1228
119,1228
91,1228
-57,8772
52,1228
50,1228
22,1228
-98,8772
-29,8772
9,1228
-18,8772
-96,8772
-27,8772
13,1228
-16,8772
-68,8772
,1228
41,1228
39,1228
*. The mean difference is significant at the 0.05 level.
Tukey HSD
Konsentrasi
ekstrak 20 mg/ml
a
Subset for alpha = 0.05
1
2
3
N
3
47,3333
ekstrak 30 mg/ml
3
49,3333
albendazole 20 mg/ml
3
77,3333
ekstrak 10 mg/ml
3
88,3333
ekstrak 5 mg/ml
3
Sig.
157,3333
,973
,053
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
83
Universitas Sumatera Utara
Lampiran 27. Data statistik kematian Pheretima posthuma
Ringkasan Jumlah Sampel
Cases
Valid
konsentrasi
N
Percent
Missing
N
Total
Percent
N
Percent
Waktu_kematian_uji_
ekstrak 5 mg/ml
3
100,0%
0
0,0%
3
100,0%
aktivitas_antelmintik
ekstrak 10 mg/ml
3
100,0%
0
0,0%
3
100,0%
ekstrak 20 mg/ml
3
100,0%
0
0,0%
3
100,0%
ekstrak 30 mg/ml
3
100,0%
0
0,0%
3
100,0%
albendazole 20 mg/ml
3
100,0%
0
0,0%
3
100,0%
Deskriptif
Konsentrasi
Waktu_kem
atian_uji_a
ktivitas_ant
elmintik
ekstrak 5 mg/ml
Mean
95% Confidence Interval for
Mean
Statistic
238,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
254,4961
238,0000
Variance
42,333
Std. Deviation
6,50641
Minimum
232,00
Maximum
245,00
Range
13,00
Interquartile Range
.
Skewness
Kurtosis
Mean
95% Confidence Interval for
Mean
,230
.
158,0000
5% Trimmed Mean
Median
Lower
Bound
Upper
Bound
171,1448
.
160,0000
28,000
Std. Deviation
5,29150
Minimum
152,00
Maximum
162,00
Range
10,00
Skewness
Kurtosis
Mean
1,225
.
3,05505
144,8552
Variance
Interquartile Range
ekstrak 20 mg/ml
222,1705
.
Median
ekstrak 10 mg/ml
Std.
Error
3,75648
.
-1,458
.
86,6667
1,225
.
2,90593
84
Universitas Sumatera Utara
95% Confidence Interval for
Mean
Lower
Bound
Upper
Bound
5% Trimmed Mean
ekstrak 30 mg/ml
99,1699
.
Median
86,0000
Variance
25,333
Std. Deviation
5,03322
Minimum
82,00
Maximum
92,00
Range
10,00
Interquartile Range
.
Skewness
Kurtosis
Mean
95% Confidence Interval for
Mean
,586
.
55,0000
Lower
Bound
Upper
Bound
5% Trimmed Mean
albendazole 20
mg/ml
74,1634
46,0433
63,9567
.
Median
56,0000
Variance
13,000
Std. Deviation
3,60555
Minimum
51,00
Maximum
58,00
Range
7,00
Interquartile Range
.
Skewness
Kurtosis
Mean
95% Confidence Interval for
Mean
-1,152
.
205,0000
5% Trimmed Mean
Median
Lower
Bound
Upper
Bound
228,6972
.
204,0000
91,000
Std. Deviation
9,53939
Minimum
196,00
Maximum
215,00
Range
19,00
Skewness
Kurtosis
1,225
.
5,50757
181,3028
Variance
Interquartile Range
1,225
.
2,08167
.
,467
.
1,225
.
Uji Normalitas
85
Universitas Sumatera Utara
KolmogorovSmirnov
a
Shapiro-Wilk
Sig
Konsentrasi
Statistic
df
.
Statistic
df
Sig.
Waktu_kematian_uji_aktivitas
ekstrak 5 mg/ml
,187
3
.
,998
3
,915
_antelmintik
ekstrak 10 mg/ml
,314
3
.
,893
3
,363
ekstrak 20 mg/ml
,219
3
.
,987
3
,780
ekstrak 30 mg/ml
,276
3
.
,942
3
,537
,208
3
.
,992
3
,826
albendazole 20
mg/ml
a. Lilliefors Significance Correction
Sig >0,05 maka dapat disimpulkan bahwa data kematian berdistribusi normal
Deskriptif
95% Confidence
Interval for Mean
Std.
N
Mean
Deviation
Std. Error
Lower
Upper
Bound
Bound
Min
Max
ekstrak 5 mg/ml
3
238,3333
6,50641
3,75648
222,1705
254,4961
232,00
245,00
ekstrak 10 mg/ml
3
158,0000
5,29150
3,05505
144,8552
171,1448
152,00
162,00
ekstrak 20 mg/ml
3
86,6667
5,03322
2,90593
74,1634
99,1699
82,00
92,00
ekstrak 30 mg/ml
3
55,0000
3,60555
2,08167
46,0433
63,9567
51,00
58,00
albendazole 20 mg/ml
3
205,0000
9,53939
5,50757
181,3028
228,6972
196,00
215,00
Total
15
148,6000
71,79017
18,53614
108,8439
188,3561
51,00
245,00
ANOVA
Sum of
Squares
Between Groups
Within Groups
Total
Uji Homogenitas
Levene
Mean
df
Square
F
71754,267
4
17938,567
399,333
10
39,933
72153,600
14
449,213
Sig.
,000
Statistic
,673
df1 df2
4
10
Sig.
,626
Perbandingan
86
Universitas Sumatera Utara
Tukey HSD
(I)
konsentrasi
ekstrak 5
mg/ml
ekstrak 10
mg/ml
ekstrak 20
mg/ml
(J) konsentrasi
ekstrak 10 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 30
mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
albendazole 20 mg/ml
albendazole
20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
Mean
Difference
Std.
(I-J)
Error
*
80,33333 5,15967
*
151,66667 5,15967
*
183,33333 5,15967
*
33,33333 5,15967
*
-80,33333 5,15967
*
71,33333 5,15967
*
103,00000 5,15967
*
-47,00000 5,15967
5,15967
*
151,66667
*
-71,33333 5,15967
*
31,66667 5,15967
5,15967
*
118,33333
5,15967
*
183,33333
5,15967
*
103,00000
*
-31,66667 5,15967
5,15967
*
150,00000
*
-33,33333 5,15967
*
47,00000 5,15967
*
118,33333 5,15967
*
150,00000 5,15967
Sig.
,000
,000
,000
,001
,000
,000
,000
,000
95% Confidence
Interval
Lower
Upper
Bound
Bound
63,3524
97,3142
134,6858 168,6476
166,3524 200,3142
16,3524
50,3142
-97,3142
-63,3524
54,3524
88,3142
86,0191 119,9809
-63,9809
-30,0191
,000 -168,6476 -134,6858
,000
,001
-88,3142
14,6858
-54,3524
48,6476
,000 -135,3142 -101,3524
,000 -200,3142 -166,3524
,000 -119,9809
-86,0191
,001
-14,6858
-48,6476
,000 -166,9809 -133,0191
,001
,000
,000
,000
-50,3142
30,0191
101,3524
133,0191
-16,3524
63,9809
135,3142
166,9809
*. The mean difference is significant at the 0.05 level.
Tukey HSD
Konsentrasi
ekstrak 30 mg/ml
N
3
3
ekstrak 10 mg/ml
3
albendazole 20
mg/ml
ekstrak 5 mg/ml
Sig.
Subset for alpha = 0.05
2
3
4
1
ekstrak 20 mg/ml
a
5
55,0000
86,6667
158,0000
3
205,0000
3
238,3333
1,000
1,000
1,000
1,000
1,000
Means for groups in homogeneous subsets are displayed.
87
Universitas Sumatera Utara
55
Universitas Sumatera Utara
Lampiran 2. Hasil identifikasi hewan
56
Universitas Sumatera Utara
Lampiran 3. Surat albendazole
57
Universitas Sumatera Utara
Lampiran 3. (Lanjutan)
Sertifikat analisis albendazole
58
Universitas Sumatera Utara
Lampiran 4. Gambar tanaman dan daun pugun tanoh [Curanga fel-terrae
(Lour.) Merr.]
Tanaman pugun tanoh
Daun pugun tanoh
59
Universitas Sumatera Utara
Lampiran 5. Gambar simplisia daun pugun aanoh dan serbuk simplisia daun
pugun tanoh
Simplisia daun pugun tanoh
Serbuk simplisia daun pugun tanoh
60
Universitas Sumatera Utara
Lampiran 6. Hasil pemeriksaan mikroskopik daun pugun tanoh
a
b
a
c
d
e
f
g
h
i
Keterangan:
a. Kristal kalsium oksalat bentuk prisma
b. Tulang daun
c. Rambut berkelenjar
d. Pembuluh angkut bentuk spiral
e. Stomata tipe anomositik
f.lStomata tipe diasitik
g. Sel tetangga
h. Epidermis bergaris
i. Trikoma
61
Universitas Sumatera Utara
Lampiran 7. Gambar seperangkat alat sokslet
Keterangan : 1: Air keluar, 2: Selang air keluar, 3: Pendingin bola, 4: Air masuk,
5: Statif, 6: Siphon sokslet, 7: Serbuk simplisia yang dibungkus
dengan kertas saring dan diikat dengan benang, 8: Selang air masuk,
9: Ember berisi air dan pompa air, 10: Labu alas bulat 500 ml, 11:
Pelarut dan batu didih, 12: Heating mantle, 13: Cok listrik, 14: Klem.
62
Universitas Sumatera Utara
Lampiran 8. Ekstrak etanol daun pugun tanoh
Lampiran 9. Pheretima posthuma
63
Universitas Sumatera Utara
Lampiran 10. Uji pengaruh etanol terhadap Pheretima posthuma
Cacing Pheretima posthuma
Tanpa perlakuan
4 % Mati
0,5 % Hidup
1% Hidup
6 % Mati
8% Mati
2% Mati
10% Mati
64
Universitas Sumatera Utara
Lampiran 11. Uji aktivitas antelmintik terhadap Pheretima posthuma
1
2
3
4
5
6
7
Keterangan : 1: dalam salin (cacing hidup), 2: dalam etanol 0,5 % (cacing hidup),
3: dalam EEDPT 5 mg/ml (cacing mati), 4: dalam EEDPT 10
mg/ml (cacing mati), 5: dalam EEDPT 20 mg/ml (cacing mati), 6:
dalam EEDPT 30 mg/ml (cacing mati), 7: Albendazole 20 mg/ml
(cacing mati).
65
Universitas Sumatera Utara
Lampiran 12. Larutan uji aktivitas antelmintik terhadap Pheretima posthuma
Keterangan: EEDPT 5 mg/ml, 10 mg/ml, 20 mg/ml, dan 30 mg/ml, larutan salin
larutan etanol 0,5 %, suspensi albendazole 20 mg/ml.
66
Universitas Sumatera Utara
67
Universitas Sumatera Utara
Lampiran 14. Perhitungan kadar sari larut air simplisia daun pugun tanoh
x 100%
% Kadar sari larut dalam air =
No.
1.
2.
3.
Berat sampel (g)
5,026
5,046
5,078
Berat sari (g)
0,173
0,175
0,164
a. % Kadar sari
=
0,173 100
x 100% 17,21%
x
20
5,026
b.% Kadar sari
=
0,175 100
x
x 100% 17,34%
5,046
20
c.% Kadar sari
=
0,164 100
x
x 100% 16,15%
5,078
20
% Rata-rata kadar sari larut dalam air =
17,21% 17,34% 16,15%
16,90%
3
68
Universitas Sumatera Utara
Lampiran 15. Perhitungan kadar sari larut etanol simplisia daun pugun tanoh
x
% Kadar sari larut dalam etanol =
No.
1.
2.
3.
Berat sampel (g)
5,011
5,018
5,008
100
20
x 100%
Berat sari (g)
0,272
0,213
0,209
a. % Kadar sari
=
0,227 100
x 100% 22,65%
x
20
5,011
b. % Kadar sari
=
0,213 100
x
x 100% 21,22%
5,018
20
c. % Kadar sari
=
0,209 100
x
x 100% 20,87%
5,008
20
%Rata-rata kadar sari larut dalam etanol =
22,65% 21,22% 20,87%
21,58%
3
69
Universitas Sumatera Utara
Lampiran 16. Perhitungan kadar abu total simplisia daun pugun tanoh
x 100%
% Kadar abu total =
No.
1.
2.
3.
Berat sampel (g)
2,0011
2,0004
2,0057
Berat abu (g)
0,0498
0,0547
0,0586
a. % Kadar abu total
=
0,0498
x 100% 2,49%
2,0011
b. % Kadar abu total
=
0,0547
x 100% 2,73%
2,0004
c. % Kadar abu total
=
0,0586
x 100% 2,92%
2,0057
% Rata-rata kadar abu total
=
= 2,71%
70
Universitas Sumatera Utara
Lampiran 17. Perhitungan kadar abu tidak larut asam simplisia daun pugun tanoh
x 100%
% Kadar abu total =
No.
1.
2.
3.
a.
Berat sampel (g)
2,0011
2,0004
2,0057
% Kadar abu total
Berat abu (g)
0,0096
0,0089
0,0077
=
0,0096
x 100% 0,48%
2,0011
b.
% Kadar abu total =
0,0089
x 100% 0,44%
2,0004
c.
% Kadar abu total =
0,0077
x 100% 0,38%
2,0057
% Rata-rata kadar abu total =
0,48% 0,44% 0,38%
0,43%
3
71
Universitas Sumatera Utara
Lampiran 18. Perhitungan kadar air EEDPT
% Kadar air ekstrak =
No.
1.
2.
3.
Berat sampel (g)
5,007
5,019
5,015
x 100%
Volume awal (ml)
1,20
1,40
1,60
% Kadar air
x 100%
=
a.% Kadar air
=
b.% Kadar air
=
c.% Kadar air
=
% Rata-rata kadar air
Volume akhir (ml)
1,40
1,60
1,80
=
1,40 - 1,20
x 100% 3,99 %
5,007
1,60 1,40
x 100% 3,98%
5,019
1,80 - 1,60
x 100% 3,99%
5,015
3,99% 3,98% 3,99%
3,99%
3
72
Universitas Sumatera Utara
Lampiran 19. Perhitungan kadar sari larut air EEDPT
x 100%
% Kadar sari larut dalam air =
No.
1.
2.
3.
Berat sampel (g)
5,007
5,016
5,011
Berat sari (g)
0,584
0,591
0,589
a.% Kadar sari
=
0,584 100
x
x 100% 58,32%
5,007
20
b.% Kadar sari
=
0,591 100
x 100% 58,91%
x
20
5,016
c.% Kadar sari
=
0,589 100
x 100% 58,77%
x
20
5,011
% Rata-rata kadar sari larut dalam air
=
58,32% 58,91% 58,77%
58,67%
3
73
Universitas Sumatera Utara
Lampiran 20. Perhitungan kadar sari larut etanol EEDPT
% Kadar sari larut dalam etanol =
No.
1.
2.
3.
Berat sampel (g)
5,025
5,012
5,030
x 100%
Berat sari (g)
0,718
0,709
0,712
a.
% Kadar sari
=
0,718 100
x
x 100% 71,44%
5,025
20
b.
% Kadar sari
=
0,709 100
x 100% 70,73%
x
20
5,012
c.
% Kadar sari
=
0,712 100
x 100% 70,77%
x
20
5,030
%Rata-rata kadar sari larut dalam etanol =
71,44% 70,73% 70,77%
70,98%
3
74
Universitas Sumatera Utara
Lampiran 21. Perhitungan kadar abu total EEDPT
x 100%
% Kadar abu total =
No.
1.
2.
3.
Berat sampel (g)
2,0050
2,0032
1,9979
Berat abu (g)
0,0384
0,0366
0,0313
0,0384
x 100% 1,91%
2,0050
a.
% Kadar abu total
=
b.
% Kadar abu total
=
0,0366
x 100% 1,83%
2,0032
c.
% Kadar abu total
=
0,0313
x 100% 1,57%
1,9979
% Rata-rata kadar abu total
=
= 1,77%
75
Universitas Sumatera Utara
Lampiran 22. Perhitungan kadar abu tidak larut asam EEDPT
x 100%
% Kadar abu total =
No.
1.
2.
3.
Berat sampel (g)
2,0050
2,0032
1,9979
Berat abu (g)
0,0070
0,0052
0,0074
a. % Kadar abu total
=
0,0070
x 100% 0,35%
2,0050
b. % Kadar abu total
=
0,0052
x 100% 0,25%
2,0032
c. % Kadar abu total
=
0,0074
x 100% 0,37%
1,9979
% Rata-rata kadar abu total
=
0,35% 0,25% 0,37%
0,32%
3
76
Universitas Sumatera Utara
Lampiran 23. Perhitungan pengenceran etanol
Pengenceran etanol 96% menjadi etanol 0,5%, 1%, 2%, 4%, 6%, 8% dan 10%
dalam 20 ml.
V1.N1=V2.N2
V2.N2
V1=
N1
20 ml x 0,5%
- Etanol 0,5 % =
0,1 ml
96%
0,1 ml etanol 96% diencerkan dengan 19,9 ml aquadest
20 ml x 1%
0,2 ml
96%
0,2 ml etanol 96% diencerkan dengan 19,8 ml aquadest
-Etanol 1 % =
20 ml x 2%
0,4 ml
96%
0,4 ml etanol 96% diencerkan dengan 19,6 ml aquadest
-Etanol 2% =
20 ml x 4%
0,8 ml
96%
0,8 ml etanol 96% diencerkan dengan 19,2 ml aquadest
20 ml x 6%
-Etanol 6 % =
1,25 ml
96%
1,25 ml etanol 96% diencerkan dengan 18,75 ml aquadest
20 ml x 8%
-Etanol 8% =
1,6 ml
96%
-Etanol 4 % =
1,6 ml etanol 96% diencerkan dengan 18,4 ml aquadest
20 ml x 10%
-Etanol 10% =
2 ml
96%
2 ml etanol 96% diencerkan dengan 18 ml aquadest
77
Universitas Sumatera Utara
Lampiran 24. Uji aktivitas antelmintik EEDPT
Waktu Paralisis Pheretima posthuma
Waktu paralisis uji aktivitas
Konsentrasi
Rata-rata
antelmintik (menit)
(mg/ml)
(menit)
I
II
II
Ekstrak 5
162
158
152
157,33
Ekstrak 10
88
95
82
88,33
Ekstrak 20
45
49
48
47,33
Ekstrak 30
50
47
51
49,33
Albendazole
74
78
80
77,33
20
Konsentrasi
(mg/ml)
Ekstrak 5
Ekstrak 10
Ekstrak 20
Ekstrak 30
Albendazole 20
Waktu kematian Pheretima posthuma
Waktu kematian uji aktivitas
Rata-rata
antelmintik (menit)
(menit)
I
II
II
238
245
232
238,33
160
162
152
158
82
92
86
86,67
56
51
58
55
215
204
196
205
SD
5,033
6,506
2,082
2,082
3,005
SD
6,506
5,292
5,033
3,605
9,539
78
Universitas Sumatera Utara
Lampiran 25. Perhitungan penyiapan ekstrak
Perhitungan konsentrasi ekstrak kental daun pugun tanoh dalam 20 ml:
-
EEDPT 5 mg/ml = 5 mg/ml x 20 ml = 100 mg
-
EEDPT 10 mg/ml = 10 mg/ml x 20 ml = 200 mg
-
EEDPT 20 mg/ml = 20 mg/ml x 20 ml = 400 mg
-
EEDPT 30 mg/ml = 30 mg/ml x 20 ml = 600 mg
-
Albendazole 20 mg/ml = 20 mg/ml x 20 ml = 400 mg
-
0,5 Tween 80 = 0,5 % x 20 ml = 0,1 ml
Keterangan : EEDPT = Ekstrak Etanol Daun Pugun Tanoh
79
Universitas Sumatera Utara
Lampiran 26. Data statistik paralisis Pheretima posthuma
Ringkasan Jumlah Sampel
Cases
Valid
Konsentrasi
N
Missing
Percent
N
Total
Percent
N
Percent
waktu_paralisis_uji_akti
ekstrak 5 mg/ml
3
100,0% 0
0,0%
3
100,0%
vitas_antelmintik
ekstrak 10 mg/ml
3
100,0% 0
0,0%
3
100,0%
ekstrak 20 mg/ml
3
100,0% 0
0,0%
3
100,0%
ekstrak 30 mg/ml
3
100,0% 0
0,0%
3
100,0%
albendazole 20 mg/ml
3
100,0% 0
0,0%
3
100,0%
Deskriptif
Std.
Konsentrasi
waktu_paralisis_uji_a
ekstrak 5 mg/ml
ktivitas_antelmintik
Mean
95% Confidence
Interval for Mean
Statistic
157,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
169,8366
158,0000
Variance
25,333
Std. Deviation
5,03322
Minimum
152,00
Maximum
162,00
Range
10,00
Interquartile Range
ekstrak 10 mg/ml
144,8301
.
Median
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
.
-,586
.
88,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
104,4961
.
88,0000
Variance
42,333
Std. Deviation
6,50641
Minimum
82,00
Maximum
95,00
Range
13,00
Skewness
Kurtosis
1,225
.
3,75648
72,1705
Median
Interquartile Range
Error
2,90593
.
,230
.
1,225
.
80
Universitas Sumatera Utara
ekstrak 20 mg/ml
Mean
95% Confidence
Interval for Mean
47,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
Median
.
2,08167
Minimum
45,00
Maximum
49,00
Range
4,00
Interquartile Range
.
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
-1,293
.
49,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
Median
44,1622
54,5045
.
4,333
Std. Deviation
2,08167
Minimum
47,00
Maximum
51,00
Range
4,00
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
.
-1,293
.
77,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
84,9225
.
78,0000
9,333
3,05505
74,00
Maximum
80,00
Range
6,00
Skewness
Kurtosis
1,225
.
1,76383
69,7442
Minimum
Interquartile Range
1,225
.
1,20185
50,0000
Variance
mg/ml
52,5045
4,333
Std. Deviation
albendazole 20
42,1622
48,0000
Variance
ekstrak 30 mg/ml
1,20185
.
-,935
.
1,225
.
Uji Normalitas
81
Universitas Sumatera Utara
Konsentrasi
Kolmogorova
Smirnov
Statistic
df
Shapiro-Wilk
Sig.
Statistic
df
Sig.
waktu_paralisis_uji_a
ekstrak 5 mg/ml
,219
3
.
,987
3
,780
ktivitas_antelmintik
ekstrak 10 mg/ml
,187
3
.
,998
3
,915
ekstrak 20 mg/ml
,292
3
.
,923
3
,463
ekstrak 30 mg/ml
,292
3
.
,923
3
,463
albendazole 20 mg/ml
,253
3
.
,964
3
,637
a. Lilliefors Significance Correction
Deskriptif
95% Confidence
Interval for Mean
Std.
N
Mean
ekstrak 5 mg/ml
3
ekstrak 10 mg/ml
Lower
Upper
Bound
Bound
Min
Max
Deviation
Std. Error
157,3333
5,03322
2,90593
144,8301
169,8366
152,00
162,00
3
88,3333
6,50641
3,75648
72,1705
104,4961
82,00
95,00
ekstrak 20 mg/ml
3
47,3333
2,08167
1,20185
42,1622
52,5045
45,00
49,00
ekstrak 30 mg/ml
3
49,3333
2,08167
1,20185
44,1622
54,5045
47,00
51,00
3
77,3333
3,05505
1,76383
69,7442
84,9225
74,00
80,00
83,9333
41,51844
10,72002
60,9412
106,9255
45,00
162,00
albendazole 20
mg/ml
Total
1
5
ANOVA
Sum of
Squares
Uji Homogenitas
Mean
df
Square
F
Sig.
Levene
Statistic
Between
Groups
Within Groups
Total
23961,600
4
5990,400
171,333
10
17,133
24132,933
14
349,634
,000
1,107
df1
df2
4
10
Sig.
,405
Perbandingan
Tukey HSD
(I)
Konsentrasi
(J) Konsentrasi
Mean
Difference
(I-J)
Std. Error
Sig.
95% Confidence
Interval
Lower
Upper
Bound
Bound
82
Universitas Sumatera Utara
ekstrak 5
mg/ml
ekstrak 10
mg/ml
ekstrak 20
mg/ml
ekstrak 30
mg/ml
albendazole
20 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
*
69,00000
*
110,00000
*
108,00000
*
80,00000
*
-69,00000
*
41,00000
*
39,00000
11,00000
*
-110,00000
*
-41,00000
-2,00000
*
-30,00000
*
-108,00000
*
-39,00000
2,00000
*
-28,00000
*
-80,00000
-11,00000
*
30,00000
*
28,00000
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
3,37968
,000
57,8772
,000
98,8772
,000
96,8772
,000
68,8772
,000
-80,1228
,000
29,8772
,000
27,8772
,053
-,1228
,000 -121,1228
,000
-52,1228
,973
-13,1228
,000
-41,1228
,000 -119,1228
,000
-50,1228
,973
-9,1228
,000
-39,1228
,000
-91,1228
,053
-22,1228
,000
18,8772
,000
16,8772
80,1228
121,1228
119,1228
91,1228
-57,8772
52,1228
50,1228
22,1228
-98,8772
-29,8772
9,1228
-18,8772
-96,8772
-27,8772
13,1228
-16,8772
-68,8772
,1228
41,1228
39,1228
*. The mean difference is significant at the 0.05 level.
Tukey HSD
Konsentrasi
ekstrak 20 mg/ml
a
Subset for alpha = 0.05
1
2
3
N
3
47,3333
ekstrak 30 mg/ml
3
49,3333
albendazole 20 mg/ml
3
77,3333
ekstrak 10 mg/ml
3
88,3333
ekstrak 5 mg/ml
3
Sig.
157,3333
,973
,053
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
83
Universitas Sumatera Utara
Lampiran 27. Data statistik kematian Pheretima posthuma
Ringkasan Jumlah Sampel
Cases
Valid
konsentrasi
N
Percent
Missing
N
Total
Percent
N
Percent
Waktu_kematian_uji_
ekstrak 5 mg/ml
3
100,0%
0
0,0%
3
100,0%
aktivitas_antelmintik
ekstrak 10 mg/ml
3
100,0%
0
0,0%
3
100,0%
ekstrak 20 mg/ml
3
100,0%
0
0,0%
3
100,0%
ekstrak 30 mg/ml
3
100,0%
0
0,0%
3
100,0%
albendazole 20 mg/ml
3
100,0%
0
0,0%
3
100,0%
Deskriptif
Konsentrasi
Waktu_kem
atian_uji_a
ktivitas_ant
elmintik
ekstrak 5 mg/ml
Mean
95% Confidence Interval for
Mean
Statistic
238,3333
Lower
Bound
Upper
Bound
5% Trimmed Mean
254,4961
238,0000
Variance
42,333
Std. Deviation
6,50641
Minimum
232,00
Maximum
245,00
Range
13,00
Interquartile Range
.
Skewness
Kurtosis
Mean
95% Confidence Interval for
Mean
,230
.
158,0000
5% Trimmed Mean
Median
Lower
Bound
Upper
Bound
171,1448
.
160,0000
28,000
Std. Deviation
5,29150
Minimum
152,00
Maximum
162,00
Range
10,00
Skewness
Kurtosis
Mean
1,225
.
3,05505
144,8552
Variance
Interquartile Range
ekstrak 20 mg/ml
222,1705
.
Median
ekstrak 10 mg/ml
Std.
Error
3,75648
.
-1,458
.
86,6667
1,225
.
2,90593
84
Universitas Sumatera Utara
95% Confidence Interval for
Mean
Lower
Bound
Upper
Bound
5% Trimmed Mean
ekstrak 30 mg/ml
99,1699
.
Median
86,0000
Variance
25,333
Std. Deviation
5,03322
Minimum
82,00
Maximum
92,00
Range
10,00
Interquartile Range
.
Skewness
Kurtosis
Mean
95% Confidence Interval for
Mean
,586
.
55,0000
Lower
Bound
Upper
Bound
5% Trimmed Mean
albendazole 20
mg/ml
74,1634
46,0433
63,9567
.
Median
56,0000
Variance
13,000
Std. Deviation
3,60555
Minimum
51,00
Maximum
58,00
Range
7,00
Interquartile Range
.
Skewness
Kurtosis
Mean
95% Confidence Interval for
Mean
-1,152
.
205,0000
5% Trimmed Mean
Median
Lower
Bound
Upper
Bound
228,6972
.
204,0000
91,000
Std. Deviation
9,53939
Minimum
196,00
Maximum
215,00
Range
19,00
Skewness
Kurtosis
1,225
.
5,50757
181,3028
Variance
Interquartile Range
1,225
.
2,08167
.
,467
.
1,225
.
Uji Normalitas
85
Universitas Sumatera Utara
KolmogorovSmirnov
a
Shapiro-Wilk
Sig
Konsentrasi
Statistic
df
.
Statistic
df
Sig.
Waktu_kematian_uji_aktivitas
ekstrak 5 mg/ml
,187
3
.
,998
3
,915
_antelmintik
ekstrak 10 mg/ml
,314
3
.
,893
3
,363
ekstrak 20 mg/ml
,219
3
.
,987
3
,780
ekstrak 30 mg/ml
,276
3
.
,942
3
,537
,208
3
.
,992
3
,826
albendazole 20
mg/ml
a. Lilliefors Significance Correction
Sig >0,05 maka dapat disimpulkan bahwa data kematian berdistribusi normal
Deskriptif
95% Confidence
Interval for Mean
Std.
N
Mean
Deviation
Std. Error
Lower
Upper
Bound
Bound
Min
Max
ekstrak 5 mg/ml
3
238,3333
6,50641
3,75648
222,1705
254,4961
232,00
245,00
ekstrak 10 mg/ml
3
158,0000
5,29150
3,05505
144,8552
171,1448
152,00
162,00
ekstrak 20 mg/ml
3
86,6667
5,03322
2,90593
74,1634
99,1699
82,00
92,00
ekstrak 30 mg/ml
3
55,0000
3,60555
2,08167
46,0433
63,9567
51,00
58,00
albendazole 20 mg/ml
3
205,0000
9,53939
5,50757
181,3028
228,6972
196,00
215,00
Total
15
148,6000
71,79017
18,53614
108,8439
188,3561
51,00
245,00
ANOVA
Sum of
Squares
Between Groups
Within Groups
Total
Uji Homogenitas
Levene
Mean
df
Square
F
71754,267
4
17938,567
399,333
10
39,933
72153,600
14
449,213
Sig.
,000
Statistic
,673
df1 df2
4
10
Sig.
,626
Perbandingan
86
Universitas Sumatera Utara
Tukey HSD
(I)
konsentrasi
ekstrak 5
mg/ml
ekstrak 10
mg/ml
ekstrak 20
mg/ml
(J) konsentrasi
ekstrak 10 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 30 mg/ml
albendazole 20 mg/ml
ekstrak 30
mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
albendazole 20 mg/ml
albendazole
20 mg/ml
ekstrak 5 mg/ml
ekstrak 10 mg/ml
ekstrak 20 mg/ml
ekstrak 30 mg/ml
Mean
Difference
Std.
(I-J)
Error
*
80,33333 5,15967
*
151,66667 5,15967
*
183,33333 5,15967
*
33,33333 5,15967
*
-80,33333 5,15967
*
71,33333 5,15967
*
103,00000 5,15967
*
-47,00000 5,15967
5,15967
*
151,66667
*
-71,33333 5,15967
*
31,66667 5,15967
5,15967
*
118,33333
5,15967
*
183,33333
5,15967
*
103,00000
*
-31,66667 5,15967
5,15967
*
150,00000
*
-33,33333 5,15967
*
47,00000 5,15967
*
118,33333 5,15967
*
150,00000 5,15967
Sig.
,000
,000
,000
,001
,000
,000
,000
,000
95% Confidence
Interval
Lower
Upper
Bound
Bound
63,3524
97,3142
134,6858 168,6476
166,3524 200,3142
16,3524
50,3142
-97,3142
-63,3524
54,3524
88,3142
86,0191 119,9809
-63,9809
-30,0191
,000 -168,6476 -134,6858
,000
,001
-88,3142
14,6858
-54,3524
48,6476
,000 -135,3142 -101,3524
,000 -200,3142 -166,3524
,000 -119,9809
-86,0191
,001
-14,6858
-48,6476
,000 -166,9809 -133,0191
,001
,000
,000
,000
-50,3142
30,0191
101,3524
133,0191
-16,3524
63,9809
135,3142
166,9809
*. The mean difference is significant at the 0.05 level.
Tukey HSD
Konsentrasi
ekstrak 30 mg/ml
N
3
3
ekstrak 10 mg/ml
3
albendazole 20
mg/ml
ekstrak 5 mg/ml
Sig.
Subset for alpha = 0.05
2
3
4
1
ekstrak 20 mg/ml
a
5
55,0000
86,6667
158,0000
3
205,0000
3
238,3333
1,000
1,000
1,000
1,000
1,000
Means for groups in homogeneous subsets are displayed.
87
Universitas Sumatera Utara