Aktifitas antibakteri dan efek terapeutik ekstrak cacinc tanah Lumbricus rabellus secara in vitro dan in vivo pada mencit berdasarkan gambaran patologi anatomi dan histopatologi
Volume b7J No. 2 Th. 2001
Cakutyoku
AKTIFITAS ANTIBAKTERl DAN EFEK TERAPEUTIK EKSTRGK CACINC
TANAH Lumbricus rubellus SECARA IN VITRO DAN IN WVO PADA MENCIT
BERDASAEUCAN GAMBARAN PATOLOGI ANATOMI DAN HISTOPATOLOGI
anaembik dan
Akh fase
5
Bambang Pontjo ~ r i o s o e r ~ a n t o "Masniari
,
~oeloen~an*',
Risa ~iurin",
Magdaleua Pancaningtyas utarni1),Yelly Afrita llyasl) dan Hendm Prasetyo ~ t o r o ' )
Laboratoriuni ~ a t o l o ~ iLaboratoriurn
'),
14elmintologi3),Fakultas Kedokteran Hewan,
Institut Pertanian Bogor, Jalan Agatis Kampus IPB Darmaga Bogor;
Laboratorium Bakteriologi, Balai Penelitian Veteriner (BALITVET), l3ogo?).
d
horn
neering. 1 5 1 160.
AJ
ABSTRACT
€A.
or
polyhydroxyalkanotes.
Mino,
50-472.
Matuso.
Satoh,
bolism
Mela-
bacterials
mhanccd
Wat.
Sci. Tech.
McCd
Feranadeq
Li.N
Polyhydroxyalkanoat
poliferation
wegarerium.
Letters 138
I
Sudiana, I.M. Mino, T.Satoh..
md Matsuo,
rRNA targeted
i
!
re-
md
nova1
biemial
:onfertnct,
G
58prosiding remu ilmiah V I PPI
20
Kaseno
1
fu
Qndreasen
iludge.
iiology
%ma
substrate
activa-ted
(9),
Wetnzel
Marais
3VR,
lumient removal
~xic
syslem:
Wafer S
an22(2),
1 19-126.
Wanner
,f
Novak
(1990).
bulk-
lum-
jur
i82.
Warer
Key words : Anllbacley,earth worm, Lumbrlcus rubellus, Airtopathology
PENDAHULUAN
?erhimpunan Pelajar
nicroauthoradiography
~ptake
filamentous
The aim of this reseach is to observe the anrtbacierial activiw and e f e c t i v i ~of earth worm Lumbricus
rubellus estrocl in vttro against Escherichia coli. S~aphylococms aurues. S~aphylococcmepidermidis,
Salmonella pullor urn and Salmonella @phi on agar cultwe, prevention and beotment efects on Salmonella
vphi-infected mice base on the macro and microscopic lesion examination of the liver, spleen, kidney, lung,
intestine, heart and brain. In vitro observation indicated [hot there was a growth inhibition efect on a11
bacteria and the highest inhibition was occured on Salmonella pulorum. The optimum worm exract concentralion on growth inhrbition wcrs 50%. The prevent ion effect was done by using the worm exrracr concentration of 12.5%; 25% and 50% to the mouse before infection with Salmonella typhi. His(opa~hologiculIy,
there was a rissue damage inhibition in all organs exomrned on all extract concenrrurion compared to the
infected-mice w ithour extract (PCU. 05) and 50% ~Jextractconcenkation gave the highesr damage inhibirion.
On the treutmenr effect a s s q , mice were infected with SalmoneiIu @phi and then treated with worm extract
Treatmen1 with worm extract could decreased the degree oftissue damaged in all concerttration compared
to the infected-mice without extract (P
Cakutyoku
AKTIFITAS ANTIBAKTERl DAN EFEK TERAPEUTIK EKSTRGK CACINC
TANAH Lumbricus rubellus SECARA IN VITRO DAN IN WVO PADA MENCIT
BERDASAEUCAN GAMBARAN PATOLOGI ANATOMI DAN HISTOPATOLOGI
anaembik dan
Akh fase
5
Bambang Pontjo ~ r i o s o e r ~ a n t o "Masniari
,
~oeloen~an*',
Risa ~iurin",
Magdaleua Pancaningtyas utarni1),Yelly Afrita llyasl) dan Hendm Prasetyo ~ t o r o ' )
Laboratoriuni ~ a t o l o ~ iLaboratoriurn
'),
14elmintologi3),Fakultas Kedokteran Hewan,
Institut Pertanian Bogor, Jalan Agatis Kampus IPB Darmaga Bogor;
Laboratorium Bakteriologi, Balai Penelitian Veteriner (BALITVET), l3ogo?).
d
horn
neering. 1 5 1 160.
AJ
ABSTRACT
€A.
or
polyhydroxyalkanotes.
Mino,
50-472.
Matuso.
Satoh,
bolism
Mela-
bacterials
mhanccd
Wat.
Sci. Tech.
McCd
Feranadeq
Li.N
Polyhydroxyalkanoat
poliferation
wegarerium.
Letters 138
I
Sudiana, I.M. Mino, T.Satoh..
md Matsuo,
rRNA targeted
i
!
re-
md
nova1
biemial
:onfertnct,
G
58prosiding remu ilmiah V I PPI
20
Kaseno
1
fu
Qndreasen
iludge.
iiology
%ma
substrate
activa-ted
(9),
Wetnzel
Marais
3VR,
lumient removal
~xic
syslem:
Wafer S
an22(2),
1 19-126.
Wanner
,f
Novak
(1990).
bulk-
lum-
jur
i82.
Warer
Key words : Anllbacley,earth worm, Lumbrlcus rubellus, Airtopathology
PENDAHULUAN
?erhimpunan Pelajar
nicroauthoradiography
~ptake
filamentous
The aim of this reseach is to observe the anrtbacierial activiw and e f e c t i v i ~of earth worm Lumbricus
rubellus estrocl in vttro against Escherichia coli. S~aphylococms aurues. S~aphylococcmepidermidis,
Salmonella pullor urn and Salmonella @phi on agar cultwe, prevention and beotment efects on Salmonella
vphi-infected mice base on the macro and microscopic lesion examination of the liver, spleen, kidney, lung,
intestine, heart and brain. In vitro observation indicated [hot there was a growth inhibition efect on a11
bacteria and the highest inhibition was occured on Salmonella pulorum. The optimum worm exract concentralion on growth inhrbition wcrs 50%. The prevent ion effect was done by using the worm exrracr concentration of 12.5%; 25% and 50% to the mouse before infection with Salmonella typhi. His(opa~hologiculIy,
there was a rissue damage inhibition in all organs exomrned on all extract concenrrurion compared to the
infected-mice w ithour extract (PCU. 05) and 50% ~Jextractconcenkation gave the highesr damage inhibirion.
On the treutmenr effect a s s q , mice were infected with SalmoneiIu @phi and then treated with worm extract
Treatmen1 with worm extract could decreased the degree oftissue damaged in all concerttration compared
to the infected-mice without extract (P