PENGARUH MEDIA BUATAN (Ekstrak Daging Cacah dan Limbah Tahu Cair) TERHADAP PERTUMBUHAN BAKTERI ANTAGONIS Ralstonia solanacearum PENYEBAB PENYAKIT LAYU PADA TEMBAKAU (Nicotiana tabaccum)

PENGARUH MEDIA BUATAN (Ekstrak Daging Cacah dan Limbah Tahu
Cair) TERHADAP PERTUMBUHAN BAKTERI ANTAGONIS Ralstonia
solanacearum PENYEBAB PENYAKIT LAYU PADA TEMBAKAU
(Nicotiana tabaccum)
Oleh: ANDINA DESI NISFUL LAIL ( 05330038 )
Biology
Dibuat: 2010-06-07 , dengan 7 file(s).

Keywords: Kata kunci : Bakteri Antagonis, R. solanacearum
ABSTRACT
Tobacco is the plantation commodity that has a strategic role in national economy, which is a
source of State revenues through foreign exchange, customs, taxes, and farmers income sources
and can create jobs. Judging from the commercial aspect, these commodities are industrial raw
materials in the country, so its presence should be maintained and further improved. Like other
plants, one obstacle to increased production of tobacco plants is a bacterial wilt disease caused
by Ralstonia solanacearum, which is one of the important diseases in plants. The use of
antagonistic microorganisms in controlling plant diseases is one way of controlling the more
economical compared to chemical control. Bacillus is one of the antagonistic microorganisms
that can be used as a barrier to the growth of R. solanacearum.
This study aims to determine the effect of artificial media for bacterial growth and antagonistic
effectiveness against R. solanacearum causes wilt disease in tobacco (Nicotiana tabaccum).

Hypothesis in this study is expected that the artificial media have an influence on bacterial
growth and effectiveness of the antagonists R. solanacearum. This research was carried out in
Balittas (Balai Penelitian Tanaman Tembakau dan Serat), on 1 July to 30 August 2009. This kind
of research is a real experiment with treatment A (control), treatment B (which had been
inoculated tobacco media and ME2 Bacillus and Bacillus isolates BK1), and treatment C
(tobacco, which has been inoculated media and TS2 Bacillus, Bacillus isolates BK1). By using a
completely randomized design (RAL) 3 times as many tests, each test sample is 5 plants. The
parameters observed were plant height measurements were observed every week. Techniques of
data analysis is done by testing a single factor analysis of variance followed by Duncan's test of
5%.
From the analysis made known that the media affect the growth of antagonistic bacteria. The
results showed that the best treatment is the treatment of B (ME2 Bacillus and Bacillus isolates
BK1). For the extension was necessary for farmers to use antagonistic microorganisms can be
immediately applied, so as not to cause so much damage to their crops.

PENGARUH MEDIA BUATAN (Ekstrak Daging Cacah dan Limbah Tahu
Cair) TERHADAP PERTUMBUHAN BAKTERI ANTAGONIS Ralstonia
solanacearum PENYEBAB PENYAKIT LAYU PADA TEMBAKAU
(Nicotiana tabaccum)
Oleh: ANDINA DESI NISFUL LAIL ( 05330038 )

Biology
Dibuat: 2010-06-07 , dengan 7 file(s).

Keywords: Kata kunci : Bakteri Antagonis, R. solanacearum
ABSTRACT
Tobacco is the plantation commodity that has a strategic role in national economy, which is a
source of State revenues through foreign exchange, customs, taxes, and farmers income sources
and can create jobs. Judging from the commercial aspect, these commodities are industrial raw
materials in the country, so its presence should be maintained and further improved. Like other
plants, one obstacle to increased production of tobacco plants is a bacterial wilt disease caused
by Ralstonia solanacearum, which is one of the important diseases in plants. The use of
antagonistic microorganisms in controlling plant diseases is one way of controlling the more
economical compared to chemical control. Bacillus is one of the antagonistic microorganisms
that can be used as a barrier to the growth of R. solanacearum.
This study aims to determine the effect of artificial media for bacterial growth and antagonistic
effectiveness against R. solanacearum causes wilt disease in tobacco (Nicotiana tabaccum).
Hypothesis in this study is expected that the artificial media have an influence on bacterial
growth and effectiveness of the antagonists R. solanacearum. This research was carried out in
Balittas (Balai Penelitian Tanaman Tembakau dan Serat), on 1 July to 30 August 2009. This kind
of research is a real experiment with treatment A (control), treatment B (which had been

inoculated tobacco media and ME2 Bacillus and Bacillus isolates BK1), and treatment C
(tobacco, which has been inoculated media and TS2 Bacillus, Bacillus isolates BK1). By using a
completely randomized design (RAL) 3 times as many tests, each test sample is 5 plants. The
parameters observed were plant height measurements were observed every week. Techniques of
data analysis is done by testing a single factor analysis of variance followed by Duncan's test of
5%.
From the analysis made known that the media affect the growth of antagonistic bacteria. The
results showed that the best treatment is the treatment of B (ME2 Bacillus and Bacillus isolates
BK1). For the extension was necessary for farmers to use antagonistic microorganisms can be
immediately applied, so as not to cause so much damage to their crops.

PENGARUH MEDIA BUATAN (Ekstrak Daging Cacah dan Limbah Tahu
Cair) TERHADAP PERTUMBUHAN BAKTERI ANTAGONIS Ralstonia
solanacearum PENYEBAB PENYAKIT LAYU PADA TEMBAKAU
(Nicotiana tabaccum)
Oleh: ANDINA DESI NISFUL LAIL ( 05330038 )
Biology
Dibuat: 2010-06-07 , dengan 7 file(s).

Keywords: Kata kunci : Bakteri Antagonis, R. solanacearum

ABSTRACT
Tobacco is the plantation commodity that has a strategic role in national economy, which is a
source of State revenues through foreign exchange, customs, taxes, and farmers income sources
and can create jobs. Judging from the commercial aspect, these commodities are industrial raw
materials in the country, so its presence should be maintained and further improved. Like other
plants, one obstacle to increased production of tobacco plants is a bacterial wilt disease caused
by Ralstonia solanacearum, which is one of the important diseases in plants. The use of
antagonistic microorganisms in controlling plant diseases is one way of controlling the more
economical compared to chemical control. Bacillus is one of the antagonistic microorganisms
that can be used as a barrier to the growth of R. solanacearum.
This study aims to determine the effect of artificial media for bacterial growth and antagonistic
effectiveness against R. solanacearum causes wilt disease in tobacco (Nicotiana tabaccum).
Hypothesis in this study is expected that the artificial media have an influence on bacterial
growth and effectiveness of the antagonists R. solanacearum. This research was carried out in
Balittas (Balai Penelitian Tanaman Tembakau dan Serat), on 1 July to 30 August 2009. This kind
of research is a real experiment with treatment A (control), treatment B (which had been
inoculated tobacco media and ME2 Bacillus and Bacillus isolates BK1), and treatment C
(tobacco, which has been inoculated media and TS2 Bacillus, Bacillus isolates BK1). By using a
completely randomized design (RAL) 3 times as many tests, each test sample is 5 plants. The
parameters observed were plant height measurements were observed every week. Techniques of

data analysis is done by testing a single factor analysis of variance followed by Duncan's test of
5%.
From the analysis made known that the media affect the growth of antagonistic bacteria. The
results showed that the best treatment is the treatment of B (ME2 Bacillus and Bacillus isolates
BK1). For the extension was necessary for farmers to use antagonistic microorganisms can be
immediately applied, so as not to cause so much damage to their crops.

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