P r o d u L s i B i o m a s s a
T a b e l 6 m e n u n ju k k a n h a liw a m ik ro a lg a
N cm m ch lo ro p sis
sp. y a n g d ik u ltiv a s i p a d a m e d ia w a ln e m e m ilik i k o n s e n tra s i b io m a s s a 0 ,2 7 g] d a n tid a k b e rb e d a n y a ta
d e n g a n k o n s e n tra s i b io m a s s a m ik ro a lg a
N arm ochloropsis
sp. y an g d ik u ltiv a s i p a d a
m e d ia g u illa rd { 0 ,2 6 g l. K o n s e n tra s i b io m a s s a m ik ro a lg a e ra t k a ita n n y a d e n g a n
k a n d u n g a n n u trie n y a n g a d a di d a la m m ed ia . M e d ia W a ln e d ik e ta h u i m e m ilik i k a n d u n g a n N d a n P y a n g lebili tin g g i d ib a n d in g k a n d e n g a n m e d ia y an g la in s e h in g g a
k o n s e n tra s i b io m a s s a n y a m e n ja d i tin g g i. P a d a m e d ia W a ln e u n s u r - u n s u r Fe, M n. Cl, d a n Z n le b ih b a n y a k te r s e d ia d a ri p a d a
m e d ia y a n g la in n y a. U n s u r - u n s u r te rs e b u t d ig u n a k a n u n tu k p ro s e s fo to s in te s is . d i m a n a h a s iln y a d ig u n a k a n u n tu k p e rtu m b u h a n F o g g . 1 9 65 . P e ra n a n u n s u r h a ra m ik ro n u trie n
d ala m laju p e rtu m b u h a n sa n g a t b esa r, y a itu b e rs a m a -s a m a m a k ro n u tr ie n m e la k u k a n p r o s e s re s p ira s i d a n m e ta b o lis m e C h ilm a w a ti d an S u m in to , 2 0 1 0 .
H as il a n a lis is ra g a m m e n u n ju k a n b a h w a p e rla k u a u je n is m e d ia b e r p e n g a r u h s a n g a t n y a ta P 0 ,0 1
te rh a d a p k a d a r le m a k m ik ro a lg a
N m n o c h lo ro p sis
sp, H a s il a n a lis is k a d a r le m a k m ik ro a lg a
N a n n o ch lo ro psis
sp. d e n g a n p e rla k u a n je n is m e d ia d a p a t d ilih a t p a d a T a b e l 7.
T a b e l 7 , K a d a r L e m a k b k
N a m o c h b r o p s is
sp. y a n g D ik u ltu r p a d a B e rb a g a i J e n is M e d ia .
J e n is M ed ia W a k tu P an en H a ri
K a d a r le m a k b k W a ln e
10 4 ,5 6 b
P e rta n ia n 8
7 ,1 6 d A lle n - M iq u e l
12 4 ,9 0 c
B lu e G r e e n - 1 1 12
9 .8 3 a G u illa rd
11 1 0 .0 0 a
K e te ra n g a n : H u r u f y a n g b e rb e d a d ib e la k a n g n ilai r a ta - r a ta m e n u n ju k k a n p e rb e d a a n y a n g n y a ta P 0 ,0 5 .
5 7
T a b d 8 m e n u n ju k k a n b a h w a k a d a r le m a k m ik io a lg a
N arm ochloropsis
sp, b e rk is a r a n ta r a 4 ,5 6 b k s a m p a i 1 0 ,0 0 b k . K ad ar le m a k m ik io a lg a
N a tm och lo ro p sis
sp. y a n g d ik u ltu r p ad a m e d ia G u illa rd s e b e s a r 1 0,03 b k , tid a k b e rb e d a n y a t a d e n g a n k a d a r
le m a k
N a m o c h lo rp sis
sp. y an g d ik u ltu r p a d a m e d ia B lu e G re e n B G - l 1 se b e s a r 9 .8 3 b k , n a m u n b e rb e d a n y a ta d e n g a n p e rla k u a n la in n y a . H a l in i s e ja la n d e n g a n p e n e litia n
Ja ti
et a l,
2 0 1 2 y a n g m e n y a ta k a n b a h w a
C ha etocero s g ra c ilis
y a n g d ik u ltiv a s i p a d a m e d ia G u illa rd m e m ilik i k a n d u n g a n a s a m le m a k o m e g a 3 E P A le b ih tin g s i 8 ,1 6
m
d ib a n d in g k a n p a d a m e d ia W a ln e 6 ,5 9 . P e n e litia n in i tid a k s a in a d e n g a n p e n e litia n S a ri
e t a l,
2 0 1 2 , y a n g m e n y a ta k a n h aliw a
N atm och lo ro psis o cu iata
y a n g d ik u ltiv a s i p a d a m e d ia A lle n -M iq u e l m e m ilik i k a n d u n g a n le m a k y a n g le b ih tin g g i 4 5
d ib a n d in g k a n d e n g a n
N atm ochlo ropsis o cuiata
y a n g d ik u ltiv a s i p a d a m ed ia G u illa r d
3 1 . W ijo s e n o 2 0 1 1 m e la p o rk a n h a liw a
C hlo rella v tilg a ris
d ik e ta h u i m e m ilik i k a d a r le m a k te rtin g g i jik a d ik u ltiv a s i p ad a m e d ia W a ln e y a itu s e b e s a r 4 2 .5 8 , p a d a m e d ia
B lu e G re e n B G - 1 1 y a itu 4 0 ,5 8 , d a n m e d ia B e n e c k y a itu 3 7 . M e n u ru t H u d a n G a o 2 0 0 6 b a h w a s e m a k in re n d a h k o n s e n tra s i n itra t d ala m b e n tu k N a N O ;, d a n fo s fa t d a ri
N a H i P 0 4 m a k a k a n d u n g a n le m a k to ta l p a d a
N a tm o ch lo ro psis
sp. se m a k in b e s a r d a n d a p a t m e n c a p a i ± 2 ,8 . P a d a m e d ia G u illa rd y a n g d ig u n a k a n d a la m p e n e litia n ini
k e te rs e d ia a n u n s u r n itra t d a n fo sfa t d a la m b e n tu k N a N O t d a n N a H ; P 0 4 le b ih s e d ik it d a rip a d a k e te r s e d ia a n u n s u r n itra t d a n fo sfa t p a d a m e d ia y a n g la in s e h in g g a k a d a r le m ak
N arotochloropsis
sp. y a n g d ik u ltu r d e n g a n m e n g g u n a k a n in ed ia G u illa rd m e n ja d i tin g g i G riffitlis d a n H a n iso n 2 0 0 9 m e n g a ta k a n b a h w a p a d a k o n d is i m e d ia d e n g a n n u trie n N
te rc u k u p i.
N m tio c h lo ro p sis
sp. m e m ilik i k a n d u n g a n le m a k to ta l b e rk is a r 2 7 - 3 1 d a n s e b a lik n y a p a d a k o n d is i k e te rb a ta s a n n u trie n N ,
N arm ochloropsis
sp. m e n g h a s ilk a n k a n d u n g a n le m a k to ta l s e b e s a r 3 5 -4 6 . P a d a m ed ia B lu e G r e e n B G -1 i m e m ilik i
k a n d u n g a n M g d a la m b e n tu k M g S 0 4. 7 H i 0 y a n g b e rp e ra n d a la m p e m b e n tu k a n lem ak , s e h in g g a k a n d u n g a n le m a k p a d a m e d ia B lu e G re e n B G - 1 1 m e n ja d i tin g g i W ijo s e n o .
58
2 0 1 1 . T in g g in v a k a d a r lip id d id u g a d is e b a b k a n o le h k o n d is i k u ltu r y a n g s tr e s s a k ib a t s u rp lu s M g
K a d a r lip id y a n g tin g g i p a d a m ik ro a lg a b ia s a n y a d ip e ro le h p a d a k o n d is i s tr e s s y a n g te rja d i b e rs a m a a n d e n g a n p e n u r u n a n laju p e rk e m b a n g b ia k a n se l P e r e z - P a z o s
2 0 1 1 . M e d ia w a ln e m e n g h a s ilk a n k a d a r le m a k p a lin g s e d ik it d ib a n d in g k a n d e n g a n
m e d ia la in n y a , k a re n a k o n s e n tra s i n itro g e n d a n fo s fa t p a d a m e d ia w a ln e y a n g d ig u n a k a n d a la n i p e n e litia n ini leb ih b a n y a k d a r ip a d a m e d ia G u illa rd , A lle n -M iq u e l, d a n P e rta n ia n .
P e n in g k a ta n N a N O ? d a n K H P 0 4 p a d a m e d ia k u ltu r a k a n m e n in g k a tk a n k a n d u n g a n p ro te in d a n
polyunsaturated fa tty acids
P U F A s
N annochloropsis,
te ta p i a k a n m e n u ru n k a n k a n d u n g a n k a rb o h id ra t, le m a k to ta l d a n
Total F atty A c ids
H u d a n G a o , 2 0 0 6 .
5 .4 . O p t im a s i N i t r a t d a n F o s f a t t e r h a d a p K o n s e n t r a s i B io m a s s a
N annochloropsis
sp .
O p tim a s i d ila k u k a n u n tu k m e n e n tu k a n titik o p tim u m n itra t d a n fo sfa t d a la m m e n g h a s ilk a n
k o n s e n tra s i b io m a s s a
m ik ro a lg a
Nannochloropsis
sp. te rtin g g i.
K o n s e n tra s i b io m a s s a m ik ro a lg a
N annochloropsis
s p d e n g a n p e rla k u a n n itra t d a n fo s fa t d is a jik a n p a d a T a b e l 8.
T a b e l 8. K o n s e n tra s i b io m a s s a m ik ro a lg a
Nannochloropsis
s p d e n g a n p e rla k u a n n itra t d a n losfat
U n it N itr a t g l
F o s fa t g l K o n s e n tra s i
b io m a s s a g l 1
5 0 5
0 .4 1 9 2
150 5
0 .6 8 9 3
50 25
0 .2 2 6 4
150 25
0 ,5 9 5 5
2 9 .3 15
0 .1 8 6 6
1 7 0.7 15
0 ,7 2 3 7
100 0 .8 6
0 ,4 7 7 8
100 2 9 ,1 4
0 .5 8 8 9
100 15
0 .5 6 6 10
100 15
0 .5 4 0 11
100 15
0 ,5 6 9 12
100 15
0 .5 8 7
5 9
B e rd a s a rk a n h a sil a n a lis is d ip e r o le h m o d e l p e rs a m a a n re g re s i s e b a g a i b e r i k u t : Z = - 0 .3 7 7 + 0 ,0 7 4 X -0 .0 0 0 2 X 2 - 0 ,0 0 8 Y -0 .0 0 2 Y 2 + 0 .0 0 0 5 X Y + 0 , d e n g a n k o e fis ie n
d e te rm in a s i r3 = 0 ,91 y a n g a rtin v a b a h w a k o n s e n tra s i n itra t d a n fo s fa t p a d a m ed ia g u illa rd m e m ilik i p e n g a ru h s e b e s a r 91 te r h a d a p k o n s e n tra s i b io m a s s a m ik ro a lg a
Narmochloropsis sp.
S is a n y a s e b a n y a k 9 d ip e n g a ru h i o le h fa k to r Iain n y a a n ta ra lain in te n s ita s c a h a y a d a n su h u .
H a s il a n a lis is j u g a m e n u n ju k k a n b a h w a p e rla k u a n n itrat d a n f o s f a t p ad a m e d ia g u illa rd te rh a d a p k o n s e n tra s i b io jn a s s a
N a n n o ch lo ro psis
sp. b e ra d a p a d a k o n d is i o p tim u m p a d a k o n s e n tr a s i n itra t 1 8 1 ,5 5 g l d a n k o n s e n tra s i fo s fa t 2 1 ,2 4 g l d e n g a n
k o n s e n tra s i b io m a s s a s e b e s a r 0 ,7 gl. P e r tu m b u h a n m ik ro a lg a d ip e n g a ru h i o le h b e rb a g a i f a k t o r S a la h s a tu fa k to r te rs e b u t a d a la h s a lin ita s . S a lin ita s m e m p e n g a ru h i m e ta b o lis m e
di d a la m se k S a lin ita s b e rp e n g a ru h te rh a d a p te k a n a n o s m o s is d a n m e k a n is m e o s m o re g u la s i y a n g s e c a ra la n g s u n g d a p a t m e m p e n g a ru h i re s p ira s i s e l m e ta b o lis m e d a n
p e m b ia k a n sel v e g e ta tif y a n g te n tu n y a a k a n m e m p e n g a ru h i k e p a d a ta n sel m ik ro a lg a V a s q u e s -D u h a lt
e af.,
1 9 9 1 . T in g g in y a s a lin ita s d a p a t m e n y e b a b k a n k e lu a rn y a a n d a ri d a la m sel s e h in g g a m e n g h a m b a t re a k s i fo to s in te s is , y an g m e ru p a k a n m e ta b o lis m e
p e n tin g b ag i m ik ro a lg a . T e r h a m b a tn y a fo to s in te s is m e n y e b a b k a n g lu k o s a y a n g te b e n tu k ju ga s e d i k i t p a d a h a l g lu k o s a d ib u tu h k a n u n tu k m e ta b o lis m e s e l te rm a s u k u n tu k s in te s is
k lo ro fil d a n
p e rk e m b a n g b ia k a n sel.
T e r h a m b a tn y a s in te s is
k lo ro fil d a n
p e r k e m b a n g b ia k a n sel m e n y e b a b k a n k o n s e n tra s i sel m e n u ru n . F a k to r lain y a n g ju g a m e m p e n g a ru h i p e rtu m b u h a n m ik ro a lg a a d a la h d e r a ja t
k e a s a m a a n p H . D e ra ja t k e a s a m a a n p H a k a n m e m p e n g a ru h i tin g k a t f o to s in te s is m ik ro a lg a S u p ra m a n ia m
et a„
2 0 1 2 , d a n k e rja e n z im d a la m p ro s e s m e ta b o lis m e sel Is n a d in a
et a l r
2 0 1 3 . P a d a p H tin g g i k e rja e n z im a k a n tid a k o p tim a l s e h in g g a re ak si m e ta b o lis m e sl tid a k b e rla n g s u n g d e n g a n b a ik , y a n g m e n g a k ib a tk a n p e rtu m b u h a n n y a
te rg a n g g u , s e h in g g a k o n s e n tr a s i sel a k a n m e n u ru n .
6 0
C a m b a r re s p o n
p e rm u k a a n d a n
c o u n te r p lo t
d a ri k o n s e n tr a s i
b io m a s s a
N otm o ch lo ro p sis
sp. d is a jik a n p a d a G a m b a r 3 d a n 4. P e ru b a h a n vvarna p a d a g ra fik d a n c o u n te r p lo t m e n u n ju k k a n a d a n y a p e rb e d a a n k a d a r le m a k d e n g a n p e rla k u a n k o m b in a s i
n itra t d a n fo s fa t y a n g b e rb e d a . W a rn a m e ra h tu a p a d a g ra fik d a n c o u n te r p lo t m e n u n ju k k a n k a d a r le m a k le b ih tin g g i d a ri 0 .7 gl, s e d a n g k a n w a r n a h ija u tu a
m e n u n ju k k a n k a d a r le m ak k u ra n g d a ri -0 .0 5 gl. • OM JurtK » M» ik w w 0»
JtMnR t «Kfri IPRum WS RMMP OCMUta OV B«mnu igl
G a m b a r 3. G ra fik r e s p o n p e rm u k a a n k o n s e n tra s i b io m a s s a
N atm och lo ro psis
sp. p e rla k u a n o p tim a s i k o n s e n tra s i n itra t d a n fo sfat
=7 F M S d w V M fe a — u ltl
J b o o « v 1 » t o t . I ? R u r « . M S I V — V u - 0 0 4 6 J M
O V B o
n n
u 0 1
G am b ar 4. C o u n te r p lo t k o n s e n tra s i
biom assa N a tm o ch lo ro psis
sp. P a d a p e rla k u a n o p tim a s i k o n s e n tra s i n itra t d a n fo s fa t
6 1
V a lid a s i m o d e l a d a la h p ro s e s u n tu k m e n e n tu k a n k e m a m p u a n m o d el k o n s e p tu a l u n tu k m e re fle k s ik a n s is te m n y a ta d e n g a n te p a t. V a lid a s i m o d e l m e ru p a k a n h a l y a n g
p e n tin g u n tu k d ila k u k a n , k a re n a s e b u a h m o d e l d a p a t d ite rim a a p a b ila m o d e l te rs e b u t te la h b e rh a s il m e le w a ti uji v a lid a s i te rle b ih d a h u lu . P a d a p e n e litia n in i, v a lid a si m o d e l
d ila k u k n n d e n g a n m e m b a n d in g k a n k o n s e n tra s i b io m a s a sel
N annochioropsis
s p s e c a ra a k tu a l d e n g a n k o n s e n tra s i b io m a s a sel
N annochioropsis
sp y a n g d ip e r o le h d a ri
p e rs a m a a n m o d e l o p tim u m y a n g d ip e ro le h d a ri o u tp u t p ro g ra m s ta tis tic a
10. P e rb a n d in g a n k o n s e n tr a s i b io m a s s a
N annochioropsis
sp d a p a t d ilih a t p a d a T a b e l 9. T a b e l
9.
P e rb a n d in g a n k o n s e n tra s i b io m a s s a
N annochioropsis
sp. a k tu a l d e n g a n m o d e l N o .
K o n s e n tra s i n itra t K o n s e n tra s i
fo s fa t R e s p o n K o n s e n tra s i B io m a s s a
gl A k tu a l
M o d e l 1
2 9 .3 0 15
0 .1 9 0 ,2 7
2 5 0 ,0 0
5 0 ,4 2
0 ,3 6 3
5 0 ,0 0 2 5
0 ,2 7 0 ,2 9
B e rd a s a rk a n lia sil v a lid a s i d a ta m e n g g u n a k a n u ji T, m a k a d ip e r o le h m lai p
O
s e b e s a r X77 d im a n a ni Jai in i leb ih b e s a r d a ri 0 .0 5 p 0 .0 5 . H a l in i m e n u n ju k k a n b a h w a tid ak ad a p e rb e d a a n n y a ta a n ta ra k o n s e n tra s i b io m a s a sel
N am ochloropsis
s p se c a ra a k tu a l d e n g a n k o n s e n tra s i b io m a s a sel
N annochioropsis
sp y a n g d ip e r o le h d a n
p e r s a m a a n m o d e l o p tim u m n y a , s e h in g g a m o d e l y a n g d ig u n a k a n u n tu k m e n g e s tim a s i k o n s e n tra s i b io m a s s a
N annochioropsis
sp. d a p a t d ite rp k a n p a d a k o n d isi n y ata.