05 Eniya Listiani Dewi Deputi TAB Presentation Aquaculture Technology in Satoumi (04102017)
FOOD AND HEALTH BASED ON
MARINE RESOURCES RESEARCH ACTIVITIES OF BPPT
Prof. Dr. Eng. Eniya Listiani Dewi, B.Eng, M.Eng
Deputy Chairman BPPT for Agroindustry and
Biotechnology
Presented at The INTERNATIONAL WORKSHOP ON SATOUMI
Jakarta, Oktober 5th, 2017
INTRODUCTION
INNOVATIIVE TECHNOLOGY
CONCLUSION
MARINE AFFAIRS AND FISHERIES IN
INDONESIA
• LARGEST ARCHIPELAGO IN THE WORLD, More Than 17.000 Islands
•
2/3 OF ITS TERRITORY IS SEA, 81.000 km Coast Line
•
HIGHLY DEPEND ON THE SEA
Nawacita (Nine Priorities Agenda)
•
•
•
•
•
•
•
•
•
Presenting back the country for the protection of the nation and security to all citizen
Promoting a clean, effective, democratic and trusted governance
Development of regional and villages area in the frame of a unitary state
System reformation and corruption free, dignified and trustworthy law enforcement
Enhancing the life quality of Indonesian people
Increase productivity and competitiveness in the international market
Promoting self-reliance economy by driving domestic economy strategic sectors
Character revolution of the nation
Affir i g the atio ’s diversity a d stre gthe i g social restoratio
MARINE AFFAIRS AND FISHERIES IN
INDONESIA
National Long Term Development Plan (RPJPN) 2005-2025:
1) National Competitiveness where as Fishery sector as economic
prime mover which produce protein food for food security and
national economic growth
2) Self reliances of national archipelago country based on national
interest by securing marine and fishery resource
3) Developing marine and fishery industry as optimal and
sustainable
• ACT NO. 32 ON MARINE WAS VOTED FOR IN SEPTEMBER 2014
• ACT NO. 45/2009 ON FISHERIES
MARINE BIOTECHNOLOGY RESEARCH
Development of Marine Biotechnology as written in the
Act No 32/2014 on Marine (article 25th and 26th)
FOOD
HEALTH
ENERGY
ENVIRONMENT
INDUSTRIAL PRODUCT & PROCESS
Aim of Marine Biotechnology Industry :
(as written in Act No. 32/2014 on marine Affairs)
1. To prevent from the extinction of marine biota due
to over exploration
2. To generate various added-value product
3. To reduce dependency on import goods by
production of import- substitute products
4. To develop environmentally friendly technology in
marine biotechnology industry
5. To develop a system for a sustainable management
of marine bio-resources
TARGET RESEARCH AREA
•
•
•
•
Genomics and Molecular Biology
Cultivation of Marine organisms
Bio-engineering of Marine Micro-organisms
Marine Model Organisms
MARINE BIODIVERSITY OF
INDONESIA
• POTENCY :
More than 415 Fish Species; 13
Seagrass,
682 Species of Seaweed, 2500 Species of
Crustacea, 745 Species of Echinodermata; etc.
POTENCY FOR FOOD
• Fish product has contributed as much as 72% as
sources of animal protein
compare to poultry (56,98%) and beef (16,18%)
• RANK 5th AS EXPORT COMMODITY
• Increasing of fish consumption in 2015 : 40,09
Kg/indv./year (5,5 % increase per year)
• Total of fish consumption demand 2015 : 10,3 Million
Ton (6,5 % increase per year)
POTENCY FOR HEALTH INDUSTRY
• Utilization of sea for pharmaceutical and cosmetics industry has not yet
optimal. In fact, the potential for pharmaceutical and cosmetic preparations
of marine Indonesia reached 9 billion US dollars or equivalent to Rp 81
trillion per year
• World demand for drugs increases of 20-30 percent per year. This
encouraged the growth of population and the type of disease. The World
Health Organization (WHO) has encouraged the development of the
pharmaceutical industry.
• If marine pharmaceutical industry is well run, the economic value generated
reached 4-5 times of the overall economic potential of fisheries,
• The utilization of potency used for the pharmaceutical and cosmetics
industry is currently only 10 percent.
INTRODUCTION
INNOVATIIVE TECHNOLOGY
CONCLUSION
TECHNOLOGYPRODUCTION
PRODUCTIONOF
OFGROUPER
GROUPERFISH
FISH
TECHNOLOGY
(Humpback
& Tiger Grouper)
(Humpback
& Tiger Grouper)
Genetic Improvement of Humpback Grouper
- Production super growth Humpback grouper
Method : selective breeding & transgenesis
Fish Disease Prevention
a. Production of Vibrio Vaccine
Method : inactivation of Vibrio spp. bacteria
b. Production of VNN Vaccine
Method: inactivation of Viral Necrosis Nervous
c. Identification and characterization of pathogen,
Method : molecular marker using 16SrRNA
Fish Feed and Nutrition
• Production feed for grow-out and brood stock
• Development of feed additive : Protein
Recombinant Growth Hormone (rGH)
Broodstock
Collection
DIALLEL CROSSING
High Salinity
Treatment
Strain-1
Male
1-S
Strain-2
2-S
3-S
4-S
5-S
6-S
7-S
8-S
1-S
Strain-3
Strain-1S
Challenge
test
Strain-5
Strain-6
35 ppt
Strain-2S
Strain-3S
2-S
Female
Strain-4
3-S
4-S
5-S
Strain-4S
Strain-7
6-2
Strain-5S
7-S
Strain-6S
8-S
Strain-8
Strain-7S
GENETIC
CHARACTERISTIC
Strain-8S
PERFORMAN TEST
GEN SALINITY
MARKING
Strain-1 : Nirwana
Strain-2 : JICA
Strain-3 : Sukabumi F3
Strain -4 : Citralada
Strain-5 : BEST
Strain-6 : Jatimbulan
Strain-7 : Red NIFI
Strain -8 : Merah
Saline tilapia :
Growth faster,
High salinity resistance
SALINE
TILAPIA
PRODUCTION TECHNOLOGY OF MARINE TILAPIA
• Next step, Salina Tilapia is to be cultured in sea waters (salinity 32 ppt)
• Solution for the limitation of freshwater and inland fish culture area
• The fillet of ari e tilapia a su stitute sea ass fillet’s
NILA GESIT (Genetically
Supermale Indonesian Tilapia) –
fresh water
Salinity (ppt)
SALINA (Saline Tolerance
Indonesian Tilapia )- 25 ppt
Maharsi (Marine Tilapia) – 32 ppt
15
PRODUCTION TECHNOLOGY OF
GROWTH HORMONE RECOMBINANT PROTEINS (rGH)
One of problem in aquaculture
activities including farming of tilapia
is the high cost of procurement of
feed that nearly 65% of the cost of
production.
Feed additive Recombinant Proteins Growth
Hormone (rGH) is very useful to accelerate the
growth of fish including tilapia and developed
through the application of recombination proteins
that can produce recombinant growth hormone in
large amounts by E. coli
To overcome these problems, it is
necessary to develop technological
breakthroughs and innovations in
the field of feed. As a solution BPPT
develop feed additive of Growth
Hormone Recombinant Protein in
which fish growth is controlled by
growth hormone (rGH).
Streptococcus DNA Vaccine
to Prevent Disease Outbreak in Tilapia Aquaculture
To increase security in aquaculture
and
reduce
imported
vaccine
products, BPPT has developed DNA
Vaccines Streptococcus. DNA
vaccines have advantages over
conventional vaccines that are
generic, safe and poses no risk of
infectious disease.
To produce the DNA Vaccine
Streptococcus, the bacteria S. inniae
isolated from organs of infected
tilapia. Then the gene was isolated
and cloned virulent streptococcus in
the
expression
vector
and
transformed into E. coli bacteria to
produce plasmid DNA as a DNA
vaccine
Integrated Multi Tropic Aquaculture
Bio-recycling System culture
Integrated Polyculture among Sea weed as Environment buffer;
Green Mussel as filter feeder; Tilapia fish (Nila Salina) and Black
tiger prawn (Penaeus monodon)
DEVELOPMENT OF DEVICES FOR TRACEABILITY OF
AQUACULTURE PRODUCT
Introduction
Fundamental traceability information is required to identify the
product and traces its physical movement through the supply chain
BPPT developed a specifically required information to be recorded
at an appropriate stages of the aquaculture product process and
supply chain
Commercially Desirable Information about the origin of the product
and the circumstances of its production that is recorded by
finfish/shellfish aquaculture companies is needed for a variety of
reasons
Part of a program for elevating global competitiveness of
aquaculture products from Indonesia
DEVELOPMENT OF DEVICES FOR TRACEABILITY
OF
AQUACULTURE
PRODUCT
Activity
- Making the traceability system to monitor, maintain, make plots, and
verify security, nutrition and quality of Indonesian fishery products;
- Making traceable entity tool kits (seed, feed, nutrition, processed
products) to support the manufacture of Indonesian fishery product
traceability systems;
- Making of Unit traceable model to the stages of production, harvest,
transport and processing
Computer
Wireless barcode scanner
barcode printer
SALINA AND MARINE TILAPIA PRODUCT
POTENTIAL AS RAW MATERIAL OF SURIMI
Tilapia
Tilapia fillet
Tilapia surimi
High Export Standard (Grade AA):
1. Gel Strength
: 457,82 gr.cm
2. Whiteness Degree : 59,94%
3. Crude Fat
: 0,44%
In comply with standard of PT. Global Seafood International Indonesia
(GSII)
PRODUCTION TECHNOLOGY OF FISH GELATIN
Fish Fillet
Extraction
Evaporation
Fish Gelatin Product
Drying & Printing
MARINE RESOURCES RESEARCH
FOR HEALTH
Searching of active compounds from marine organisms (Sponges,
Marine microbes and Microbial Symbiosis in the sponge)
Applied Research Activities :
• Isolation and Fermentation of Marine Microbial
• Screening activities of some Marine Microbial Extracts
• Isolation of active compounds, structure elucidation and bioassay
Production Technology Development of Polyunsaturated Fatty
Acid (PUFA) From Microalgae
Other source of DHA should
be found
Fish Oil is susceptible to
oxidation and unpleasant
fishy taste
MICROALGAE
Difficult to purify DHA
like compounds
DHA content
MARINE RESOURCES RESEARCH ACTIVITIES OF BPPT
Prof. Dr. Eng. Eniya Listiani Dewi, B.Eng, M.Eng
Deputy Chairman BPPT for Agroindustry and
Biotechnology
Presented at The INTERNATIONAL WORKSHOP ON SATOUMI
Jakarta, Oktober 5th, 2017
INTRODUCTION
INNOVATIIVE TECHNOLOGY
CONCLUSION
MARINE AFFAIRS AND FISHERIES IN
INDONESIA
• LARGEST ARCHIPELAGO IN THE WORLD, More Than 17.000 Islands
•
2/3 OF ITS TERRITORY IS SEA, 81.000 km Coast Line
•
HIGHLY DEPEND ON THE SEA
Nawacita (Nine Priorities Agenda)
•
•
•
•
•
•
•
•
•
Presenting back the country for the protection of the nation and security to all citizen
Promoting a clean, effective, democratic and trusted governance
Development of regional and villages area in the frame of a unitary state
System reformation and corruption free, dignified and trustworthy law enforcement
Enhancing the life quality of Indonesian people
Increase productivity and competitiveness in the international market
Promoting self-reliance economy by driving domestic economy strategic sectors
Character revolution of the nation
Affir i g the atio ’s diversity a d stre gthe i g social restoratio
MARINE AFFAIRS AND FISHERIES IN
INDONESIA
National Long Term Development Plan (RPJPN) 2005-2025:
1) National Competitiveness where as Fishery sector as economic
prime mover which produce protein food for food security and
national economic growth
2) Self reliances of national archipelago country based on national
interest by securing marine and fishery resource
3) Developing marine and fishery industry as optimal and
sustainable
• ACT NO. 32 ON MARINE WAS VOTED FOR IN SEPTEMBER 2014
• ACT NO. 45/2009 ON FISHERIES
MARINE BIOTECHNOLOGY RESEARCH
Development of Marine Biotechnology as written in the
Act No 32/2014 on Marine (article 25th and 26th)
FOOD
HEALTH
ENERGY
ENVIRONMENT
INDUSTRIAL PRODUCT & PROCESS
Aim of Marine Biotechnology Industry :
(as written in Act No. 32/2014 on marine Affairs)
1. To prevent from the extinction of marine biota due
to over exploration
2. To generate various added-value product
3. To reduce dependency on import goods by
production of import- substitute products
4. To develop environmentally friendly technology in
marine biotechnology industry
5. To develop a system for a sustainable management
of marine bio-resources
TARGET RESEARCH AREA
•
•
•
•
Genomics and Molecular Biology
Cultivation of Marine organisms
Bio-engineering of Marine Micro-organisms
Marine Model Organisms
MARINE BIODIVERSITY OF
INDONESIA
• POTENCY :
More than 415 Fish Species; 13
Seagrass,
682 Species of Seaweed, 2500 Species of
Crustacea, 745 Species of Echinodermata; etc.
POTENCY FOR FOOD
• Fish product has contributed as much as 72% as
sources of animal protein
compare to poultry (56,98%) and beef (16,18%)
• RANK 5th AS EXPORT COMMODITY
• Increasing of fish consumption in 2015 : 40,09
Kg/indv./year (5,5 % increase per year)
• Total of fish consumption demand 2015 : 10,3 Million
Ton (6,5 % increase per year)
POTENCY FOR HEALTH INDUSTRY
• Utilization of sea for pharmaceutical and cosmetics industry has not yet
optimal. In fact, the potential for pharmaceutical and cosmetic preparations
of marine Indonesia reached 9 billion US dollars or equivalent to Rp 81
trillion per year
• World demand for drugs increases of 20-30 percent per year. This
encouraged the growth of population and the type of disease. The World
Health Organization (WHO) has encouraged the development of the
pharmaceutical industry.
• If marine pharmaceutical industry is well run, the economic value generated
reached 4-5 times of the overall economic potential of fisheries,
• The utilization of potency used for the pharmaceutical and cosmetics
industry is currently only 10 percent.
INTRODUCTION
INNOVATIIVE TECHNOLOGY
CONCLUSION
TECHNOLOGYPRODUCTION
PRODUCTIONOF
OFGROUPER
GROUPERFISH
FISH
TECHNOLOGY
(Humpback
& Tiger Grouper)
(Humpback
& Tiger Grouper)
Genetic Improvement of Humpback Grouper
- Production super growth Humpback grouper
Method : selective breeding & transgenesis
Fish Disease Prevention
a. Production of Vibrio Vaccine
Method : inactivation of Vibrio spp. bacteria
b. Production of VNN Vaccine
Method: inactivation of Viral Necrosis Nervous
c. Identification and characterization of pathogen,
Method : molecular marker using 16SrRNA
Fish Feed and Nutrition
• Production feed for grow-out and brood stock
• Development of feed additive : Protein
Recombinant Growth Hormone (rGH)
Broodstock
Collection
DIALLEL CROSSING
High Salinity
Treatment
Strain-1
Male
1-S
Strain-2
2-S
3-S
4-S
5-S
6-S
7-S
8-S
1-S
Strain-3
Strain-1S
Challenge
test
Strain-5
Strain-6
35 ppt
Strain-2S
Strain-3S
2-S
Female
Strain-4
3-S
4-S
5-S
Strain-4S
Strain-7
6-2
Strain-5S
7-S
Strain-6S
8-S
Strain-8
Strain-7S
GENETIC
CHARACTERISTIC
Strain-8S
PERFORMAN TEST
GEN SALINITY
MARKING
Strain-1 : Nirwana
Strain-2 : JICA
Strain-3 : Sukabumi F3
Strain -4 : Citralada
Strain-5 : BEST
Strain-6 : Jatimbulan
Strain-7 : Red NIFI
Strain -8 : Merah
Saline tilapia :
Growth faster,
High salinity resistance
SALINE
TILAPIA
PRODUCTION TECHNOLOGY OF MARINE TILAPIA
• Next step, Salina Tilapia is to be cultured in sea waters (salinity 32 ppt)
• Solution for the limitation of freshwater and inland fish culture area
• The fillet of ari e tilapia a su stitute sea ass fillet’s
NILA GESIT (Genetically
Supermale Indonesian Tilapia) –
fresh water
Salinity (ppt)
SALINA (Saline Tolerance
Indonesian Tilapia )- 25 ppt
Maharsi (Marine Tilapia) – 32 ppt
15
PRODUCTION TECHNOLOGY OF
GROWTH HORMONE RECOMBINANT PROTEINS (rGH)
One of problem in aquaculture
activities including farming of tilapia
is the high cost of procurement of
feed that nearly 65% of the cost of
production.
Feed additive Recombinant Proteins Growth
Hormone (rGH) is very useful to accelerate the
growth of fish including tilapia and developed
through the application of recombination proteins
that can produce recombinant growth hormone in
large amounts by E. coli
To overcome these problems, it is
necessary to develop technological
breakthroughs and innovations in
the field of feed. As a solution BPPT
develop feed additive of Growth
Hormone Recombinant Protein in
which fish growth is controlled by
growth hormone (rGH).
Streptococcus DNA Vaccine
to Prevent Disease Outbreak in Tilapia Aquaculture
To increase security in aquaculture
and
reduce
imported
vaccine
products, BPPT has developed DNA
Vaccines Streptococcus. DNA
vaccines have advantages over
conventional vaccines that are
generic, safe and poses no risk of
infectious disease.
To produce the DNA Vaccine
Streptococcus, the bacteria S. inniae
isolated from organs of infected
tilapia. Then the gene was isolated
and cloned virulent streptococcus in
the
expression
vector
and
transformed into E. coli bacteria to
produce plasmid DNA as a DNA
vaccine
Integrated Multi Tropic Aquaculture
Bio-recycling System culture
Integrated Polyculture among Sea weed as Environment buffer;
Green Mussel as filter feeder; Tilapia fish (Nila Salina) and Black
tiger prawn (Penaeus monodon)
DEVELOPMENT OF DEVICES FOR TRACEABILITY OF
AQUACULTURE PRODUCT
Introduction
Fundamental traceability information is required to identify the
product and traces its physical movement through the supply chain
BPPT developed a specifically required information to be recorded
at an appropriate stages of the aquaculture product process and
supply chain
Commercially Desirable Information about the origin of the product
and the circumstances of its production that is recorded by
finfish/shellfish aquaculture companies is needed for a variety of
reasons
Part of a program for elevating global competitiveness of
aquaculture products from Indonesia
DEVELOPMENT OF DEVICES FOR TRACEABILITY
OF
AQUACULTURE
PRODUCT
Activity
- Making the traceability system to monitor, maintain, make plots, and
verify security, nutrition and quality of Indonesian fishery products;
- Making traceable entity tool kits (seed, feed, nutrition, processed
products) to support the manufacture of Indonesian fishery product
traceability systems;
- Making of Unit traceable model to the stages of production, harvest,
transport and processing
Computer
Wireless barcode scanner
barcode printer
SALINA AND MARINE TILAPIA PRODUCT
POTENTIAL AS RAW MATERIAL OF SURIMI
Tilapia
Tilapia fillet
Tilapia surimi
High Export Standard (Grade AA):
1. Gel Strength
: 457,82 gr.cm
2. Whiteness Degree : 59,94%
3. Crude Fat
: 0,44%
In comply with standard of PT. Global Seafood International Indonesia
(GSII)
PRODUCTION TECHNOLOGY OF FISH GELATIN
Fish Fillet
Extraction
Evaporation
Fish Gelatin Product
Drying & Printing
MARINE RESOURCES RESEARCH
FOR HEALTH
Searching of active compounds from marine organisms (Sponges,
Marine microbes and Microbial Symbiosis in the sponge)
Applied Research Activities :
• Isolation and Fermentation of Marine Microbial
• Screening activities of some Marine Microbial Extracts
• Isolation of active compounds, structure elucidation and bioassay
Production Technology Development of Polyunsaturated Fatty
Acid (PUFA) From Microalgae
Other source of DHA should
be found
Fish Oil is susceptible to
oxidation and unpleasant
fishy taste
MICROALGAE
Difficult to purify DHA
like compounds
DHA content