Lampiran 1.Surat persetujuan etik
LAMPIRAN
Lampiran 1.Surat persetujuan etik
Lampiran 2.Surat keterangan determinasi
Lampiran 3. Bagan rancangan dosis pada sighting study Perhitungan dosis uji pendahuluan
1. Dosis = 300 mg/kg BB BB hewan uji = 125 gram = 0,125 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji
2. Dosis = 300 mg/kg BB BB hewan uji = 130 gram = 0,130 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji
3. Dosis = 300 mg/kg BB BB hewan uji = 145 gram = 0,145 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji, tidak
ditemukan kematian pada hewan uji, sehingga pada hari berikutnya dosis
dinaikan menjadi 2000 mg/kg BB1. Dosis = 2000 mg/kg BB BB hewan uji = 145 gram = 0,145 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji
2. Dosis = 2000 mg/kg BB BB hewan uji = 125 gram = 0,125 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji
3. Dosis = 2000 mg/kg BB BB hewan uji = 130 gram = 0,130 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji, tidak
terjadi kematian, sehingga pada main study digunakan dosis 2000 mg/kg
BBLampiran 4. Pengamatan Gejala Efek toksik
X X
X X
X X
X X Diare
X X
X X
X X tinjaberdarah
X X
X X Kerontokanbulu
X X
X X
X X
X X Kulitkemerahan bengkak
X X
X X
X X
X X
X X
X X muntah
X X
Dosis 300 mg/kg BB Dosis 2000 mg/kg bb
X X
1
2
3
1
2
3 kegelisahan
X X
X X keberangasan
X X kebingungan
X X
X X
X X agresif
X X
X X
X X ketakutan
X X
X X
X X
lampiran 5. Gambar klasifikasi GHS
Lampiran 6. Bagan rancangan dosis pada main study Data perhitungan dosis pada uji utama (main study) pada kelompok perlakuan
1. Dosis = 2000 mg/kg BB BB hewan uji = 155 gram = 0,155 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji
2. Dosis = 2000 mg/kg BB BB hewan uji = 123 gram = 0,123 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji
3. Dosis = 2000 mg/kg BB BB hewan uji = 130 gram = 0,130 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji
4. Dosis = 2000 mg/kg BB BB hewan uji = 179 gram = 0,179 kg
Diambil 2 mL untuk di suntikan secara peroral pada hewan uji
5. Dosis = 2000 mg/kg BB BB hewan uji = 160 gram = 0,160 kg Diambil 2 mL untuk di suntikan secara peroral pada hewan uji.
Lampiran 7. Hasil pengamatan gejala efek toksik selama 14 hari
No h-1 h+1 h+2 h+3 h+4 h+5 h+6 h+7 h+8 h+9 h+10 h+11 h+12 h+13 h+14
X X
X X
X X
8 X
X X
X X
X X
X X
X X
X X
X X
7 X
X X
X X
X X
X X
X X
X X
X X
X X
10 X
X X Ket: kelompok kontrol : tikus no 6-10, kelompok perlakuan: tikus no 1-5
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
9 X
X X
X X
6 X
1 X
X X
X X
3 X
X X
X X
X X
X X
X X
X X
X X
2 X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
X X
5 X
X X
X X
X X
X X
X X
X X
X X
4 X
X X
X X
TOKSISITAS AKUT EKSTRAK…, FITRI KURNIAWATI, FAKULTAS FARMASI UMP, 2014
Lampiran 8. Data berat badan (gram) hewan uji selama 14 hari
tikus No h-1 h+1 h+2 h+3 h+4 h+5 h+6 h+7 h+8 h+9 h+10 h+11 h+12 h+13 h+14 1 160 173 167 182 183 186.5 190 189 185 181 185 192 190 192 195
2 127 180 180 200 204 197 191.5 198 191.5 189 194 200 203 200 190 3 136 197 164 182 195 197 199 184 184 184 188 185 209 189 184 4 183 136 138 155 159 159.5 163 159 161 156 163 165 186 209 200 5 163 187 183 200 209 211.5 213.5 214.5 200 198 206 201 165 189 173 6 126 154 141 147 157 151.5 146 156 149 144 154 154 153 155 149 7 150 196 165 179 183 189 195 188 185 174 194 194 197 195 189 8 127.5 140 160 174.5 173 176 179 180.5 177 168 162.5 155 179 172 182 9 164 195 137 153 156 159 159 156 154 150 159 152 155 150 154
10 130 174.5 161 173 180 182.5 185 179 176.5 174 173 177 185 187.5 182
TOKSISITAS AKUT EKSTRAK…, FITRI KURNIAWATI, FAKULTAS FARMASI UMP, 2014
Lampiran 9. Data hasil jumlah eritrosit (mm
3 ) tikus no h-1 h+1 h+7 h+14 1 7600000 6000000 11200000 6400000
2 8400000 12000000 12800000 8800000 3 8350000 10400000 16000000 7200000 4 9250000 5400000 24000000 8800000 5 6500000 6600000 8800000 5600000 6 5500000 10400000 7500000 4000000 7 9300000 11200000 25000000 6400000 8 8780000 6000000 17600000 9600000 9 7000000 11200000 20000000 4000000
10 9080000 12000000 10200000 8000000 tikus no h-1 h+1 h+7 h+14 1 3000 7850 9600 6400 2 6100 7500 7050 7800 3 6500 9600 7900 8200 4 3600 6400 6750 7650 5 3400 6750 9050 9600 6 4500 9050 9800 9600 7 5500 7650 6950 6800 8 5200 6900 9600 6400 9 5650 7050 7850 7650
10 5950 7900 8800 7950 Lampiran 10. Data hasil jumlah leukosit (mm
3 )
Lampiran 11. Data hasil pengukuran enzim GPT (U/L) tikus no h-1 h+1 h+7 h+17
1
69.4
71.1 73.9 123.4
2
66.4 173.3 122.1 102.7
3
71.3 124.8 117.9 101.9
4 102.1 13797.1
60.5 5 120.5 86.4 125.9 107.8
6
77 107.2 117.7 116.3
7
69
84.7 209.4
79.7
8
77.4 111.6 131.1 110.4
9 41.2 101.1
99.6
92
10
67.6
86.2 141.4
75.5
Lampiran 12. Data hasil penguukuran enzim GOT (U/L) tikus no h-1 h+1 h+7 h+14
34.5
37
43.2
22.2
7
18.1
26.1
54.5
13.4
8
23.12
31
6
23
9
22.3
27
15.5
21.3
10
24
24.9
32.1
18.2
25.8
1
19.4
64.4
64.9
29.7
28
2
17.1
44.7
34.3
27
3
38.6
20.9
31.2
42.8
4
16.9
21
26.7
21.3
5
48.7
23.4
23.6
Lampiran 13. Data hasil pengukuran urea darah (mg/dL) Hewan uji h-1 h+1 h+7 h+14
37
35
35
23
7
48
28
29
23
8
31
26
6
31
9
42
33
35
36
10
24
23
32
29
36
1
23
21
25
35
28
2
48
26
31
26
3
38
39
36
23
4
40
33
29
25
5
52
95
28
Lampiran 14. Data hasil penimbangan bobot organ hati dan ginjal
tikus no hati ginjal Kanan kiri 1 7.547 0.492 0.502
2 7.427 0.616 0.609 3 7.369 0.622 0.602 4 7.762 0.583 0.578 5 6.553 0.555 0.511 6 9.149 0.661 0.646 7 7.676 0.561 0.533 8 6.753 0.518 0.417 9 6.124 0.524 0.518
10 6.932 0.518 0.501
Lampiran 15. Hasil uji histopatologi
Lampiran 16. Gambar hasil pengumpulan dan penyiapan ekstrak Proses pengeringan Daun telah kering
Pengayakan Penimbangan
Maserasi Serbuk kering Penguapan Pengadukan Ekstrak etanol daun binahong
Lampiran 17. Gambar Penyiapan hewan uji Penyimpanan hewan uji Penyuntikan secara peroral
Pengamatan gejala efek toksik Pengambilan darah
Lampiran 18. Gambar penyiapan uji biokimia Spektrofotometer Layar spektrofotometer
Persiapan uji biokimia Plasma EDTA
Lampiran 19. Gambar Pembedahan Alat bedah
Pembedahan Pengambilan organ Euthensia Pengawetan organ hati Pengawetan organ ginjal
Lampiran 20. Hasil Analisis Statistik Analisis statistik berat badan 1.
a. Uji T berpasangan
Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 153,80 5 22,42 10,02
Minggu1 184,10 5 17,65 7,89
Paired Samples Correlations N Correlation Sig.
Pair 1 hmin1 & minggu1 5 -0,617 0,268
Paired Samples Test
Paired Differences 95% Confidence Interval of the Difference Std. Error Sig. (2-Mean Std. Deviation Mean Lower Upper t df tailed)
- – Pair 1 hmin1
- 30,30 36,09 16,14 -75,11 14.51 -1.87
4 0,13 minggu1 Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 153,80 5 22,42 10,02
Minggu1 188,11 5 6,73 3,01
Paired Samples Correlations N Correlation Sig.
Pair 1 hmin1 & minggu1 5 -0,84 0,06
b. Analisis uji T berpasangan
7.41 Levene's Test for
39.49
37.71 Equal variances not assumed 2,40 5,28 0.05 19,26 8,00 -0.96
0.81
8 0,04 19,26 8,00
0,59 0,46 1,54 8 0,62 14,21 9,22 -7,05 35,47 Equal variances not assumed 1,51 6,57 0,17 14,21 9,22 -7,89 36,31 minggu2 Equal variances assumed 5,17 0,05 2,4
8 0,28 14,30 12,52 -14,58 43,18 Equal variances not assumed 1,14 7,4 0,28 14,30 12,52 -14,98 43,58 minggu1 Equal variances assumed
Interval of the Difference Lower Upper hmin1 Equal variances assumed 0,521 0,49 1,14
Difference Std. Error Difference 95% Confidence
Equality of Variances t-test for Equality of Means F Sig. T df Sig. (2- tailed) Mean
Group Statistics
kelompokuji N Mean Std. Deviation Std. Error Mean hmin1 Perlakuan 5 153,80
3.01 nonperlakuan 5 168,85
6.73
4.76 minggu2 Perlakuan 5 188,11
10.65
7.89 nonperlakuan 5 169,89
17.65
7.50 minggu1 Perlakuan 5 184,10
16.78
10.02 nonperlakuan 5 139,50
22.42
16.57
2. Hasil analisis statistik sel darah merah
a. Uji T tidak berpasangan jumlah
Group Statistics
kelompokuji N Mean Std. Deviation Std. Error Mean hmin1 perlakuan 5 8,0200E6 1,03114E6 4,61140E5 nonperlakuan 5 7,9320E6 1,63491E6 7,31153E5 hplus1 perlakuan 5 8,0800E6 2,93462E6 1,31240E6 nonperlakuan 5 1,0160E7 2,39332E6 1,07033E6 hplus7 perlakuan 5 1,4560E7 5,88965E6 2,63393E6 nonperlakuan 5 1,6060E7 7,16645E6 3,20493E6 hplus14 perlakuan 5 7,3600E6 1,43108E6 6,40000E5 nonperlakuan 5 6,4000E6 2,46577E6 1,10272E6
Independent Samples Test
Levene's Test for Equality ofVariances t-test for Equality of Means F Sig. t df Sig. (2- tailed)
Mean Differen ce Std. Error
Difference 95% Confidence Interval of the Difference
Lower Upper hmin1 Equal variances assumed 2,25 0,17 0,10 8 0,92 8,80E4 8,64E5 -1,91E6 2,08E6 Equal variances not assumed 0,10 6,74 0,92 8,80E4 8,64E5 -1,97E6 2,15E6 hplus1 Equal variances assumed 1,12 0,32 -1,22 8 0,25 -2,08E6 1,69E6 -5,98E6 1,82E6 Equal variances not assumed -1,22 7,68 0,25 -2,08E6 1,69E6 -6,01E6 1,82E6 hplus7 Equal variances assumed 0,48 0,50 -0.36 8 0,72 -1,5E6 4,14E6 -1,10E7 8,06E6 Equal variances not assumed -0.36 7,71 0,72 -1,5E6 4,15E6 -1,11E7 8,13E6 hplus14 Equal variances assumed 1,58 0,24 0,75 8 0,47 9,6E5 1,27E6 -1,98E6 3,90E6 Equal variances not assumed 0,75 6,42 0,47 9,6E5 1,27E6 -2,11E6 4.036E6
b. Uji T berpasangan
95% Confidence Interval of the Difference Lower Upper
Pair 1 hmin1 & hplus7 5 0,889 0,044
Paired Samples Correlations N Correlation Sig.
5 5,89E6 2,63E6
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 8,02E6 5 1,03E6 4,61E5 hplus7 1,46E7
Paired Samples Statistics
Pair 1 hmin1 - hplus1
Std. Error Mean
Paired Samples Statistics
Mean tailed) Std. Deviation
Paired Differences t df Sig. (2-
Paired Samples Test
Pair 1 hmin1 & hplus1 5 0,17 0,782
Paired Samples Correlations N Correlation Sig.
5 2,93E6 1,31E6
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 8,02E6 5 1,03E6 4,61E5 hplus1 8,08E6
- 6,00E4 2,94E6 1,31E6 -3,71E6 3,59E6 -0,04 4 0,966
Paired Samples Test
Paired Differences 95% Confidence Interval of the Difference
Std. Std. Error Sig. (2- Mean Deviation Mean Lower Upper t df tailed)
Pair 1 hmin1 -
- 6,54E6 4,99E6 2,23E6 -1,27E7 -3,37E5 -2,98 4 0,043 hplus7
Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 8,02E6
5
1.03E6
4.61E5 hplus14 7,36E6
5
1.43E6
6.40E5
Paired Samples Correlations N Correlation Sig.
Pair 1 hmin1 & hplus14 5 0,90 0,034
Paired Samples Test
Paired Differences 95% Confidence
Interval of the Difference
Std. Std. Error Sig. (2- Mean Deviation Mean Lower Upper t df tailed)
Pair 1 hmin1 - hplus14 6,60E5 6,63E5 2,96E5 -1,63E5 1,48E6 2,22 4 0,090
3. Hasil analisis statistik jumlah sel darah putih
a. Uji T tidak berpasangan
Group Statistics
kelompokuji N Mean Std. Deviation Std. Error Mean hplus1 Perlakuan 5 7,62E3 1248,29 558,26 nonperlakuan 5 7,71E3 855,42 382,56 hmin1 Perlakuan 5 4,52E3 1645,29 735,80 nonperlakuan 5 5,36E3 551,58 246,68 hplus7 Perlakuan 5 8,07E3 1236,22 552,86 nonperlakuan 5 8,60E3 1200,52 536,89 hplus14 Perlakuan 5 7,93E3 1150,87 514,68 nonperlakuan 5 7,68E3 124,27 555,57
Independent Samples Test
Levene's Test for Equality ofVariances t-test for Equality of Means F Sig. t df Sig. (2- tailed)
Mean Difference Std. Error Differenc e 95% Confidence
Interval of the Difference Lower Upper hplus1 Equal variances assumed 0,44 0,53 -0,13
8 0,90 -90,00 676,76 -1650,60 1470,60 Equal variances not assumed -0,13 7,08 0,90 -90,00 676,76 -1686,70 1506,71 hmin1 Equal variances assumed 19,22 0,02 -1,08 8 0,31 -840,00 776,05 -2629,56 949,57 Equal variances not assumed -1,08 4,89 0,33 -840,00 776,05 -2848,72 1168,73 hplus7 Equal variances assumed 0,02 0,90 -0,69 8 0,51 -530,00 770,65 -2307,12 1247.,29 Equal variances not assumed -0,69 7,99 0,51 -530,00 770,65 -2307,38 1247,38 hplus14 Equal variances assumed .043 .840 0,33 8 0,75 250,00 757,33 -1496,41 1996,41 Equal variances not assumed 0,33 7,954 0,75 250,00 757,33 -1498,18 1998,19
b. Uji T berpasangan
95% Confidence Interval of the Difference Lower Upper
Pair 1 hmin1 & hplus7 5 -0,51 0,379
Paired Samples Correlations N Correlation Sig.
5 1236,22 552,87
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 4,52E3 5 1645,36 735,80 hplus7 8,07E3
Paired Samples Statistics
Pair 1 hmin1 - hplus1
Std. Error Mean
Paired Samples Statistics
Mean tailed) Std. Deviation
Paired Differences t df Sig. (2-
Paired Samples Test
Pair 1 hmin1 & hplus1 5 0,667 0,219
Paired Samples Correlations N Correlation Sig.
5 1248,30 558,26
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 4,52E3 5 1645,30 735,80 hplus1 7,62E3
- 3,10E3 1235,42 552,50 -4633,97 -1566,03 -5,61 4 0,005
Paired Samples Test
Paired Differences 95% Confidence Interval of the Difference
Std. Std. Error Sig. (2- Mean Deviation Mean Lower Upper t df tailed)
Pair 1 hmin1 -
- 3,41E3 1873,63 837,91 -5736,42 -1083.58 -4,07 4 0,015 hplus14
Paired Differences
95% Confidence Interval of the Difference Std. Std. Error Sig. (2-Mean Deviation Mean Lower Upper t df tailed) Pair 1 hmin1 - hplus7 -3,55E3 2512,72 1123,72 -6669,95 -430,05 -3,159 4 0,034
Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 4,52E3 5 1645,30 735,80 hplus14 7,93E3
5 1150,87 514,68
Paired Samples Correlations N Correlation Sig.
Pair 1 hmin1 & hplus14 5 0,138 0,825
4. Hasil analisis statistic enzim GPT
a. Uji T tidak berpasangan
Group Statistics
kelompokuji N Mean Std. Deviation Std. Error Mean hmin1 Perlakuan 5 33,30 21,96 9,82 nonperlakuan 5 21,14 2,80 1,25 hplus1 Perlakuan 5 38,52 17,81 7,97 nonperlakuan 5 29,90 5,46 2,44 hplus7 Perlakuan 5 28,56 5,08 2,27 nonperlakuan 5 35,26 14,59 6,52 hplus14 Perlakuan 5 28,98 8,14 3,63 nonperlakuan 5 20,70 4,17 1,86
Independent Samples Test
Levene's Test for Equality ofVariances t-test for Equality of Means F Sig. t df Sig. (2- tailed)
Mean Differen ce Std.
Error Differen ce 95% Confidence
Interval of the Difference Lower Upper hmin1 Equal variances assumed 26,02 0,00 1.228
8 0,25 12,16 9,90 -10,67 34,99 Equal variances not assumed 1.228 4,13 0,28 12.16 9,90 -14,99 39,30 hplus1 Equal variances assumed 3,28 0,11 1.034
8 0,33 8,62 8,33 -10,60 27,84 Equal variances not assumed 1.034 4,75 0,35 8,62 8,33 -13,15 30,39 hplus7 Equal variances assumed 3,42 0,10 -0,970 8 0,36 -6,70 6,91 -22,64 9,24 Equal variances not assumed -0,970 4,96 0,38 -6,70 6,91 -24,51 11,11 hplus14 Equal variances assumed 0,98 0,35 2,024 8 0,08 8,28 4,09 -1,15 17,71 Equal variances not assumed 2,024 5,97 0,09 8,28 4,09 -174 18,30
b. Uji T berpasangan
95% Confidence Interval of the Difference Lower Upper
Pair 1 hmin1 & hplus7 5 -0,407 0,497
Paired Samples Correlations N Correlation Sig.
5 5,08 2,27
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 33,30 5 21,96 9,82 hplus7 28,56
Paired Samples Statistics
Pair 1 hmin1 - hplus1 -5,22 20,32 9,09 -30,45 20,01 -0,574 4 0,596
Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 33,30 5 21,96 9,82 hplus1 38,52
Mean tailed) Std. Deviation
Paired Differences t df Sig. (2-
Paired Samples Test
Pair 1 hmin1 & hplus1 5 0,494 0,397
Paired Samples Correlations N Correlation Sig.
5 17.82 7,96
Std. Error Mean
Paired Samples Test
5 8,14 3,64
Paired Samples Correlations N Correlation Sig.
Pair 1 hmin1 - hplus14 4,32 24,60 11,00 -26.23 34,87 0,39 4 0,715
95% Confidence Interval of the Difference Lower Upper
Std. Error Mean
Paired Differences t df Sig. (2-tailed) Mean Std. Deviation
Paired Samples Test
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 33,30 5 21,96 9,82 hplus14 28,98
Paired Differences t df Sig. (2-
Paired Samples Statistics
Pair 1 hmin1 - hplus7 4,74 24,47 10,94 -25,65 35,13 0,433 4 0,687
Difference Lower Upper
95% Confidence Interval of the
Std. Error Mean
Mean tailed) Std. Deviation
Pair 1 hmin1 & hplus14 5 -0,159 0,799
5. Analisis statistic enzim GOT
a. Uji T tidak berpasangan
Group Statistics
kelompokuji N Mean Std. Deviation Std. Error Mean hmin1 Perlakuan 5 85,94 24,11 10,78 nonperlakuan 5 66,44 14,80 6,62 hplus1 Perlakuan 5 118,5 40,81 18,25 nonperlakuan 5 98,16 12,20 5,45 hplus7 Perlakuan 5 107,38 21,78 9,73 nonperlakuan 5 139,84 41,91 18,74 hplus14 Perlakuan 5 99,26 23,33 10,43 nonperlakuan 5 94,78 18,12 8,10
Independent Samples Test
Levene's Test for Equality ofVariances t-test for Equality of Means F Sig. t df Sig.
(2- tailed) Mean Differenc e Std.
Error Differenc e 95% Confidence
Interval of the Difference Lower Upper hmin1 Equal variances assumed 3,28 0,11 1,54
8 0,16 19,50 12,65 -9,68 48,67 Equal variances not assumed 1,54 6,64 0,17 19,50 12,65 -10,75 49,75 hplus1 Equal variances assumed 5,58 0,05 1,07 8 0,32 20,36 19,04 -23,56 64,28 Equal variances not assumed 1,06 4,71 0,34 20,36 19,04 -29,53 70,25 hplus7 Equal variances assumed 0,71 0,42 -1,53 8 0,16 -32,46 21,12 -81,17 16,25 Equal variances not assumed -1,53 6,01 0,17 -32,46 21,12 -84,11 19,19 hplus14 Equal variances assumed 0,00 0,93 0,34 8 0,74 4,48 13,21 -25,98 34,94 Equal variances not assumed 0,34 7,54 0,74 4,48 13,21 -26,31 35,27
b. Uji T berpasangan
Difference Lower Upper
Pair 1 hmin1 & hplus7 5 0,279 0,649
Paired Samples Correlations N Correlation Sig.
5 21,77 9,74
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 85,94 5 24,11 10,78 hplus7 107,38
Paired Samples Statistics
Pair 1 hmin1 - hplus1 -32,58 53,30 23,83 -98,76 33,60 -1,36 4 0,244
Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 85,940 5 24,11 10,78 hplus1 118,52
Std. Error Mean
Mean tailed) Std. Deviation
Paired Differences t df Sig. (2-
Paired Samples Test
Pair 1 hmin1 & hplus1 5 -0,302 0,621
18.25 Paired Samples Correlations N Correlation Sig.
5 40,81
95% Confidence Interval of the
Paired Samples Test
Paired Differences 95% Confidence
Interval of the Difference
Std. Std. Error Sig. (2- Mean Deviation Mean Lower Upper t df tailed)
Pair 1 hmin1 - hplus7 -21,44 27,61 12,35 -55,73 12,85 -1,73 4 0,158
Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 85,94 5 24,11 10,78 hplus14 99,26
5 23,33 10,43
Paired Samples Correlations N Correlation Sig.
Pair 1 hmin1 & hplus14 5 -0,372 0,538
Paired Samples Test
Paired Differences 95% Confidence Interval of the Difference
Std. Std. Error Sig. (2- Mean Deviation Mean Lower Upper t df tailed)
Pair 1 hmin1 -
- 13,32 39,28 17,57 62,10 35,46 -0,75 4 0,491 hplus14
6. Analisis statistik urea darah
a. Uji T tidak berpasangan
Group Statistics
kelompokuji N Mean Std. Deviation Std. Error Mean hmin1 Perlakuan 5 36,80 14,20 6,35 nonperlakuan 5 34,80 9,88 4,42 hplus1 Perlakuan 5 43,40 29,33 13,11 nonperlakuan 5 31,20 5,67 2,53 hplus7 Perlakuan 5 27,60 5,03 2,24 nonperlakuan 5 31,40 3,91 1,74 hplus14 Perlakuan 5 34,00 4,00 1,78 nonperlakuan 5 28,20 5,54 2,47
Independent Samples Test
Levene's Test for Equality ofVariances t-test for Equality of Means F Sig. t df Sig. (2- tailed)
Mean Difference Std.
Error Differenc e 95% Confidence
Interval of the Difference Lower Upper hmin1 Equal variances assumed 1,654 0, 234 0,25
8 0,80 2,00 7,73 -15,84 19,84 Equal variances not assumed 0,25 7,13 0,80 2,00 7,73 -16,22 20,22 hplus1 Equal variances assumed 3,871 0,085 0,91 8 0,38 12,20 13,36 -18,60 43,00 Equal variances not assumed 0,91 4,29 0,40 12,20 13,36 -23,89 48,29 hplus7 Equal variances assumed 0,062 0,810 -1,33 8 0,21 -3,80 2,84 -10,37 2,77 Equal variances not assumed -1,33 7,54 0,22 -3,80 2,84 -10,44 2,84 hplus14 Equal variances assumed 0,473 0,511 1,89 8 0,09 5,80 3,05 -1,24 12,84 Equal variances not assumed 1,89 7,27 0,09 5,80 3,05 -1,37 12,97
b. Uji T berpasangan
Difference Lower Upper
Pair 1 hmin1 & hplus7 5 0,55 0,331
Paired Samples Correlations N Correlation Sig.
5 5,02 2,24
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 36,80 5 14,20 6,35 hplus7 27,60
Paired Samples Statistics
Pair 1 hmin1 - hplus1 -6,60 24,52 10,96 -37,04 23,84 -0,60 4 0,580
Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 36,80 5 14,20 6,35 hplus1 43,40
Std. Error Mean
Mean tailed) Std. Deviation
Paired Differences t df Sig. (2-
Paired Samples Test
Pair 1 hmin1 & hplus1 5 0,553 0,334
Paired Samples Correlations N Correlation Sig.
5 29,33 13,11
95% Confidence Interval of the
Paired Samples Test
Paired Differences 95% Confidence
Interval of the Difference
Std. Std. Error Sig. (2- Mean Deviation Mean Lower Upper t df tailed)
Pair 1 hmin1 - hplus7 9,20 12,15 5,43 -5,89 24,29 1,69 4 0,166
Paired Samples Statistics
Mean N Std. Deviation Std. Error Mean Pair 1 hmin1 36,80 5 14,20 6,35 hplus14 34,00
5 4,00 1,78
Paired Samples Correlations N Correlation Sig.
Pair 1 hmin1 & hplus14 5 -0,075 0,905
Paired Samples Test
Paired Differences 95% Confidence
Interval of the Difference
Std. Std. Error Sig. (2- Mean Deviation Mean Lower Upper t df tailed)
Pair 1 hmin1 - 2,80 15,03 6,72 -15,87 21,47 0,416 4 0,699 hplus14
7. Hasil analisis statistik bobot organ ginjal kanan (uji t tidak berpasangan)
Group Statistics
kelompokuji N Mean Std. Deviation Std. Error Mean bobotorgan perlakuan 5 0,57 0,05 0,02 nonperlakuan 5 0,55 0,06 0,02
Independent Samples Test
Levene's Test for Equality of Variances t-test for Equality of Means
F Sig. t df Sig.
(2- tailed) Mean
Differen ce Std.
Error Differen ce
95% Confidence
Interval of the Difference
Lower Upper bobotorgan Equal variances assumed
0,03 0,867 0,47 8 0,64 0,01 0,03 -0,06 0,10 Equal variances not assumed
0,47 7,84 0,64 0,01 0,03 -0,06 0,10
8. Hasil analisis statistik bobot organ ginjal kiri ( uji T tidak berpasangan)
Group Statistics
Kelompokuji N Mean Std. Deviation Std. Error Mean bobotorgan Perlakuan 5 0,56 0,05 0,02
Nonperlakuan 5 0,52 0,08 0,03
Independent Samples Test
Levene's Test for Equality of Variances t-test for Equality of Means
F Sig. t df Sig.
(2- tailed) Mean
Difference Std. Error
Difference 95% Confidence
Interval of the Difference
Lower Upper bobotorgan Equal variances assumed 0,147 0,711 0,86 8 0,41 0,03 0,04 -0,06 0,13
Equal variances not assumed 0,86 6,65 0,41 0,03 0,04 -0,06 0,14
9. Hasil analisis statistik bobot organ hati ( uji T tidak berpasangan)
T-Test
Group Statistics
Kelompokuji N Mean Std. Deviation Std. Error Mean bobotorgan Perlakuan 5 7,33 0,46 0,20
Nonperlakuan 5 7,32 1,15 0,51
Independent Samples Test
Levene's Test for Equality of
Variances t-test for Equality of Means 95%
Confidence Std. Interval of the
Sig. Mean Error Difference
(2- Differen Differenc F Sig. t df tailed) ce e Lower Upper bobotorgan Equal variances assumed 3,158 0,11 0,00 8 0,993 0,00 0,55 -1,28 1,29
Equal variances not 0,00 5,22 0,993 0,00 0,55 -1,41 1,41 assumed