PENGARUH PEREBUSAN TERHADAP AKTIVITAS ANTIOKSIDAN, KANDUNGAN VITAMIN C, DAN TOTAL FENOL PADA CAISIM (Brassica rapa parachinensis) - Unika Repository

  Lampiran 1. KURVA STANDAR VITAMIN C y = -0,018x + 0,711 R² = 0,98

  0,1 0,2 0,3 0,4 0,5 0,6 0,7 0,8

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  44 Ab s o rb a n s i

  Konsentrasi asam askorbat (ppm) Series1 Linear (Series1)

  Perpustakaan Unika

  Perpustakaan Unika Lampiran 2. KURVA STANDAR TOTAL FENOL

  1,2

  1 0,8 si n a rb

  0,6 so b

  Series1 A

  0,4 Linear (Series1) y = 0,011x + 0,041

  0,2 R² = 0,989

  10

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  50

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  70

  80 90 100 Konsentrasi quercetin (ppm)

  Lampiran 3. HASIL OBSERVASI Nama rumah makan jenis pengolahan waktu perebusan perlakuan sebelum direbus rumah makan Pak bambang sup, capcai kuah, bakso kuah

  30 menit dicuci, dipotong, dihilangkan bagian yang rusak rumah makan "24 jam" sup, capcai, bakso kuah 15 menit dicuci, dipotong- potong rumah makan

  "MM" sup, capcai kuah, sawi putih rebus, caisim rebus 15 menit dihilangkan bagian yang rusak, dicuci, dipotong-potong rumah makan "Pentul" sup, capcai kuah, caisim rebus

  12-15 menit dicuci, dipotong- potong rumah makan bu Minah sup, capcai kuah, Ca sawi

  10-15 menit dicuci, dipotong- potong rumah makan "Langsam" sup, Ca sawi, capcai kuah, bakso

  15 menit dicuci, dipotong- potong Perpustakaan Unika

  Perpustakaan Unika Lampiran 4. HASIL PENELITIAN PENDAHULUAN o

  a. Perebusan dengan suhu 100 C Kandungan vitamin C Lama perebusan pada caisim

  (menit) (mg/100 g bahan) 32,665 ± 0,142

  15 5,312 ± 0,312 35 1,089 ± 0,072 60 1,484 ± 0,126

  Keterangan: Kandungan vitamin C merupakan nilai mean standar deviasi ± o

  b. Perebusan dengan suhu 70 C Kandungan vitamin C Kandungan vitamin C Lama perebusan pada air rebusan caisim pada caisim

  (menit) (mg/100 g bahan) (mg/100 g bahan) 0 - 29,764 ± 1,342

  15 2,311 ± 0,079 13,732 ± 1,312 35 1,026 8,653 ± 0,122 ± 1,072 60 0,921 ± 0,089 4,972 ± 0,526

  Keterangan: Kandungan vitamin C merupakan nilai mean standar deviasi ±

  Lampiran

  Aktivitas antioksidan

  N Mean Std.

  Deviation Std.

  Error 95% Confidence Interval for Mean Minimum Maximum Lower

  Bound Upper Bound 6 28.78107 1.754034 .716082 26.94032 30.62182 27.025 30.948

  3 6 26.39586 1.307018 .533588 25.02423 27.76749 24.779 27.916 6 6 22.32015 2.694634 1.100080 19.49231 25.14800 19.423 25.045 9 6 21.27858 .499533 .203933 20.75435 21.80281 20.513 21.921 12 6 17.94129 5.381466 2.196974 12.29379 23.58879 12.013 23.098 15 6 16.78364 5.611089 2.290718 10.89516 22.67211 10.954 23.487 20 6 13.97965 3.879607 1.583843 9.90826 18.05105 9.912 18.252 25 6 11.66637 .876994 .358031 10.74602 12.58672 10.235 12.815 35 6 8.41011 1.815412 .741139 6.50495 10.31527 5.966 11.014 45 6 8.07634 .858728 .350574 7.17516 8.97752 7.018 9.270 60 6 3.52716 .946954 .386592 2.53339 4.52093 2.358 4.795 Total

66 16.28729 8.149255 1.003104 14.28396 18.29063 2.358 30.948

  Descriptives

  vitaminC

  N Mean Std.

  Deviation Std. Error 95% Confidence Interval for Mean Minimum Maximum Lower

  Bound Upper Bound 6 29.80911 .361532 .147595 29.42970 30.18851 29.167 30.090

  3 6 28.54135 .423754 .172997 28.09664 28.98605 27.899 29.071 6 6 25.89217 .829774 .338754 25.02137 26.76296 24.852 26.758 9 6 23.11694 1.084576 .442776 21.97875 24.25513 21.757 24.166 12 6 19.83097 1.393126 .568742 18.36897 21.29296 18.212 21.744 15 6 19.07250 2.225870 .908708 16.73659 21.40840 16.743 21.499 20 6 17.59854 .633579 .258658 16.93364 18.26344 16.573 18.247 25 6 16.51366 .803110 .327868 15.67085 17.35647 15.554 17.902 35 6 11.72313 .363264 .148302 11.34191 12.10436 11.139 12.197 45 6 7.60291 .968135 .395240 6.58692 8.61891 6.020 8.590 60 6 2.38616 .756157 .308700 1.59262 3.17970 1.185 3.174 Total

66 18.37158 8.295434 1.021097 16.33231 20.41086 1.185 30.090

  Perpustakaan Unika

5. UJI SPSS DATA PENGUJIAN KANDUNGAN SENYAWA FUNGSIONAL PADA CAISIM SELAMA PEREBUSAN Descriptives

  Descriptives

  2 21.912716 472.33824 584.99510

  00

  12 6 1224.60000 89.70377

  9 36.621415

  1130.4616

  6 1318.73834

  1084.4

  00 1348.4

  00

  15 6 824.13333 63.53010

  8 25.936058 757.46257 890.80409

  729.60 920.80

  20 6 528.66667 53.67497

  467.20 615.20

  1096.8

  25 6 624.26667 67.79057

  9 27.675388 553.12482 695.40852

  529.20 736.40

  35 6 384.33333 94.36895

  38.525962 285.29919 483.36747 250.00 510.80

  45 6 306.40000 61.40749

  1 25.069503 241.95679 370.84321

  244.80 400.40

  60 6 269.46667 51.79879

  21.146768 215.10717 323.82616 214.00 364.80

  Total 66 942.53333 549.6584

  29 67.658276 807.41043 1077.65624

  214.00 2023.2

  00 1310.8

  8 1305.50312

  total_fenol N Mean Std.

  46 44.732886

  Deviation Std. Error 95% Confidence Interval for Mean

  Minim um Maxim um

  Lower Bound

  Upper Bound 6 1590.33333

  65.09336

  9 26.574257

  1522.0220

  3 1658.64463

  1508.8

  00 1684.4

  00

  3 6 1847.06667 109.5727

  1732.0771

  1135.6968

  2 1962.05621

  1736.0

  00 2023.2

  00

  6 6 1548.00000 96.48079

  6 39.388120

  1446.7496

  1 1649.25039

  1404.8

  00 1671.6

  00

  9 6 1220.60000 80.90359

  7 33.028755

  00 Perpustakaan Unika

  Perpustakaan Unika

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  60 akt_antioksidan vitamin_C Fenol Statistic df Sig. Statistic df Sig.

  Kolmogorov-Smirnov

  a

  Shapiro-Wilk This is a lower bound of the true significance.

  Lilliefors Significance Correction a.

  45

  25

  Tests of Normality

  25

  .272 6 .188 .845 6 .144 .206 6 .200* .907 6 .418 .267 6 .200* .803 6 .062 .177 6 .200* .965 6 .858 .295 6 .113 .790 6 .047 .270 6 .196 .844 6 .142 .294 6 .114 .800 6 .059 .251 6 .200* .944 6 .694 .168 6 .200* .984 6 .970 .252 6 .200* .917 6 .486 .171 6 .200* .950 6 .743 .263 6 .200* .826 6 .099 .163 6 .200* .967 6 .869 .263 6 .200* .871 6 .231 .257 6 .200* .831 6 .110 .184 6 .200* .946 6 .711 .277 6 .166 .815 6 .080 .170 6 .200* .931 6 .588 .241 6 .200* .930 6 .583 .240 6 .200* .943 6 .683 .226 6 .200* .917 6 .483 .165 6 .200* .938 6 .643 .176 6 .200* .908 6 .424 .204 6 .200* .955 6 .778 .233 6 .200* .938 6 .646 .202 6 .200* .930 6 .578 .200 6 .200* .924 6 .532 .216 6 .200* .877 6 .257 .234 6 .200* .928 6 .568 .189 6 .200* .975 6 .926 .173 6 .200* .973 6 .909 .240 6 .200* .832 6 .112 .211 6 .200* .973 6 .915 waktu

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• .

  ANOVA

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  N

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5

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  8

  9 Subset for alpha = .05 Means for groups in homogeneous subsets are displayed.

  Uses Harmonic Mean Sample Size = 6.000.

  a.

  Perpustakaan Unika

  12

  20

  3849.221 10 384.922 45.290 .000 467.452 55 8.499

  Post Hoc Tests Homogeneous Subsets akt_antioksidan Duncan a

  4316.673

  65 4414.135 10 441.414 412.963 .000

  58.789 55 1.069 4472.924

  65 9093.734 10 909.373 260.817 .000

  191.765 55 3.487 9285.499

  65 Between Groups Within Groups Total Between Groups Within Groups Total Between Groups Within Groups Total akt_antioksidan vitamin_C Fenol

  Sum of Squares df Mean Square F Sig.

  6 3.52716 6 8.07634 6 8.41011 8.41011 6 11.66637 11.66637 6 13.97965 13.97965 6 16.78364 16.78364 6 17.94129 17.94129

  25

  6 21.27858 21.27858

  6 22.32015

  6 26.39586

  6 28.78107 1.000 .844 .058 .175 .101 .494 .052 .539 .162 waktu

  60

  45

  35

3 Sig.

  vitamin_C Duncan a

3 Sig.

  N

  6 69.38030 .246 1.000 1.000 .074 .081 1.000 1.000 1.000 waktu

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  1

  6 54.42727

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  7

  8 Subset for alpha = .05 Means for groups in homogeneous subsets are displayed.

  Uses Harmonic Mean Sample Size = 6.000.

  a.

  Perpustakaan Unika

  6 60.88030

  6 30.96818 6 32.23333 6 35.38939 6 38.32727 6 40.99697 6 42.96212 6 50.23182 6 52.14697

  6 2.38616 6 7.60291 6 1.72313 6 6.51366 6 7.59854 6 9.07250 6 9.83097 6 23.11694

  6

  6 25.89217

  6 28.54135

  6 29.80911 1.000 1.000 1.000 .075 .209 1.000 1.000 1.000 1.000 waktu

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  N

  Fenol Duncan a

  1

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5

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  7

  8

  9 Subset for alpha = .05 Means for groups in homogeneous subsets are displayed.

  Uses Harmonic Mean Sample Size = 6.000.

  a.

3 Sig.

  Perpustakaan Unika Lampiran

6. UJI SPSS DATA PENGUJIAN KANDUNGAN SENYAWA FUNGSIONAL PADA AIR REBUSAN CAISIM Descriptives

  Aktivitas antioksidan Std. Std. 95% Confidence

  N Mean Deviation Error Interval for Mean Minimum Maximum Lower Upper

  Bound Bound

  3 6 .48088 .692929 .282887 -.24631 1.20806 -.209 1.671 9 6 3.11057 .627286 .256088 2.45227 3.76886 2.294 3.897 15 6 3.45376 .658526 .268842 2.76268 4.14484 2.454 4.428 35 6 2.72863 .661464 .270042 2.03446 3.42279 1.700 3.374 60 6 1.42938 .636924 .260023 .76097 2.09779 .624 2.390 Total 30 2.24064 1.287624 .235087 1.75983 2.72145 -.209 4.428

  Descriptives

  Vitamin C Std. Std. 95% Confidence

  N Mean Deviation Error Interval for Mean Minimum Maximum Lower Upper

  Bound Bound

  3 6 4.44299 .489060 .199658 3.92975 4.95622 3.541 4.931 9 6 5.35228 .775133 .316447 4.53882 6.16573 4.450 6.116 15 6 4.45319 .338900 .138355 4.09753 4.80884 3.921 4.874 35 6 2.44372 1.563019 .638100 .80343 4.08400 .249 4.267 60 6 1.29472 1.137800 .464505 .10067 2.48877 -.437 2.356 Total 30 3.59738 1.765808 .322391 2.93801 4.25674 -.437 6.116

  Descriptives

  Total fenol Std. 95% Confidence

  N Mean Deviation Std. Error Interval for Mean Minimum Maximum Lower Upper

  Bound Bound

  3 6 48.13333 28.706143 11.719234 18.00808 78.25858 3.600 82.000 9 6 104.29333 38.465999 15.703678 63.92574 144.66092 54.000 138.000 15 6 4.53333 2.935757 1.198518 1.45245 7.61422 1.200 9.600 35 6 134.66667 3.314614 1.353186 131.18819 138.14514 130.000 139.200 60 6 219.00000 38.133030 15.567744 178.98184 259.01816 183.600 272.800 Total

  Perpustakaan Unika Tests of Normality a

  Kolmogorov-Smirnov Shapiro-Wilk

waktu Statistic df Sig. Statistic df Sig.

akt_antioksidan

  3 .167 6 .200* .919 6 .497

  9 .237 6 .200* .902 6 .388

  15 .208 6 .200* .974 6 .918

  35 .194 6 .200* .917 6 .485

  60 .158 6 .200* .974 6 .916 vitamin_C

  3 .265 6 .200* .873 6 .240 9 .298 6 .105 .793 6 .051 15 .176 6 .200* .975 6 .923

  35 .207 6 .200* .948 6 .721

  60 .266 6 .200* .878 6 .258 fenol

  3 .181 6 .200* .964 6 .853

  9 .272 6 .188 .822 6 .091

  15 .239 6 .200* .931 6 .589

  35 .199 6 .200* .970 6 .890

  60 .312 6 .070 .819 6 .086 *.

  This is a lower bound of the true significance.

  a.

  Lilliefors Significance Correction ANOVA

  Sum of Squares df Mean Square F Sig. akt_antioksida Between Group 37.329

  4 9.332 21.698 .000 Within Groups 10.753 25 .430 Total 48.081

  

29

vitamin_C Between Group 66.962 4 16.740 17.838 .000 Within Groups 23.462 25 .938 Total 90.424

  

29

fenol Between Group 84.182 4 21.045 53.931 .000 Within Groups 9.756

  25 .390 Total 93.938

  

29

  Perpustakaan Unika Post Hoc Tests Homogeneous Subsets akt_antioksidan a

  Duncan Subset for alpha = .05 waktu N

  1

  2

  3

  3 6 .48088 60 6 1.42938 35 6 2.72863

  9 6 3.11057 15 6 3.45376 Sig.

  

1.000 1.000 .081

Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 6.000. vitamin_C a

  Duncan Subset for alpha = .05 waktu N

  1

  2

  60 6 1.29472 35 6 2.44372 3 6 4.44299 15 6 4.45319 9 6 5.35228 Sig.

  .051 .136 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  Perpustakaan Unika fenol a

  Duncan Subset for alpha = .05 waktu N

  1

  2

  3

  4

  15 6 .10303 3 6 1.09394

  9 6 2.37030 35 6 3.06061

  60

  6 4.97727 Sig.

  

1.000 1.000 .067 1.000

Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  Perpustakaan Unika

  15

  Lilliefors Significance Correction a.

  Shapiro-Wilk This is a lower bound of the true significance.

  a

  Kolmogorov-Smirnov

  60 kadar_air Statistic df Sig. Statistic df Sig.

  45

  35

  25

  20

  12

  Lampiran 7. UJI SPSS DATA KADAR AIR PADA CAISIM SELAMA PEREBUSAN Descriptives

  9

  6

  3

  .136 6 .200* .975 6 .922 .315 6 .063 .861 6 .191 .299 6 .102 .768 6 .030 .209 6 .200* .956 6 .789 .262 6 .200* .814 6 .077 .181 6 .200* .972 6 .903 .305 6 .086 .792 6 .049 .283 6 .146 .795 6 .052 .304 6 .088 .726 6 .012 .284 6 .142 .880 6 .269 .164 6 .200* .934 6 .611 waktu

  Tests of Normality

  3 6 92.59000 1.952096 .796940 90.54140 94.63860 89.554 94.470 6 6 91.88333 2.118060 .864694 89.66057 94.10610 88.008 93.328 9 6 91.77367 1.379962 .563367 90.32549 93.22185 89.529 93.676 12 6 93.73117 .282731 .115425 93.43446 94.02787 93.461 94.049 15 6 93.69883 .625448 .255338 93.04247 94.35520 92.906 94.662 20 6 91.67200 1.965476 .802402 89.60936 93.73464 87.895 93.372 25 6 92.46367 2.642863 1.078944 89.69015 95.23718 87.502 94.470 35 6 92.79900 4.038148 1.648567 88.56122 97.03678 84.864 95.560 45 6 95.06367 1.251033 .510732 93.75079 96.37655 93.781 97.303 60 6 94.66317 .710154 .289919 93.91791 95.40843 93.869 95.644 Total 66 93.15127 2.172571 .267425 92.61719 93.68536 84.864 97.303

  Upper Bound 6 94.32550 1.997086 .815307 92.22969 96.42131 91.921 97.303

  Lower Bound

  Deviation Std. Error 95% Confidence Interval for Mean Minimum Maximum

  kdr_air N Mean Std.

• .

  ANOVA

  35

  a.

  Uses Harmonic Mean Sample Size = 6.000.

  2 Subset for alpha = .05 Means for groups in homogeneous subsets are displayed.

  1

  N

  45 Sig.

  60

  12

  15

  3

  kadar_air 87.382 10 8.738 2.190 .032

  25

  6

  9

  20

  6 91.67200 6 91.77367 6 91.88333 6 92.46367 92.46367 6 92.59000 92.59000 6 92.79900 92.79900 6 93.69883 93.69883 6 93.73117 93.73117 6 94.32550 94.32550 6 94.66317 6 95.06367 .054 .057 waktu

  Post Hoc Tests Homogeneous Subsets kadar_air Duncan a

  Sum of Squares df Mean Square F Sig.

  65 Between Groups Within Groups Total

  219.422 55 3.989 306.804

  Perpustakaan Unika

  Perpustakaan Unika

LAMPIRAN 8. PERSIAPAN SAMPEL CAISIM SEBELUM ANALISA

  a. Potongan caisim segar

  b. Proses perebusan

  c. Caisim yang dihaluskan

  d. Caisim setelah direbus