Barcoding of grouperspecies in Spermonde Archipelago, South Sulawesi.
South Sulawesi
Barcoding of grouper species in Spermonde Archipelago,
Nuryanto, A.t, Pramono H'1, Kochzius M'2
1
lndonesia
Faculty Biology, Jenderal Soedirman University, Purwokerto'
zMarine Biology, Vrije Universiteit Brussel' Belgium
-
corresponding author: anuryanto2d)3@yahoo'com'
Abstract
marine live trade with high price' This
lndonesia,s groupers are marketed in the international
illegally fished specimens cannot be
leads to over exploitation due to illegal fishing. The
during sampling' in order to
identified properly due to time limitation provided by fisherman
is also known as DNA barcoding allows
avoid that the fish die. Molecular identification, which
is a prerequisite for
to identify any living organisms with high precision. such information
marker for molecular identification of
management. Cytocliro*" C oxidase I (COl) ii a standard
grouper species harvested in the spermonde
animars. The objective of the project is to identify
using the big-dye terminator
Archipelago. The amplified col fragments were sequenced
BioEdit (ver' 7'0'4'1)' A
method. All sequences were edited manually using the softeware
implemented in BioEdit (ver'
multiple sequences alignment was done using ClustalW as
available in the barcoding database
7.O. .tl.The orthologylo pr"uious published sequences
verified. To support this result' a
BOLD (http:/lboldsystems.org) and GenBank were also
programme MEGA (v' A'0) using teh
phylogenetic tree was reconstructed with the help of the
bootstrap replicates' A total of 28
neighbaur joining (NJ) method with 10fr) non-parametric
identification proved that only one
sequences were obtained during the study. Molecular
with Epinephelus ongus' The remaining
sequences matched well t99.8%) in the BOLD data base
and proved that the sequences
27 sequences were blasted to the data available in GenBank
genus Epinephelus consist of eight
were homologous with Epfne phelus and Plectropomus. The
consist of one individual of
individuals of Epinephelus ongus, while the genus Plectropomus
plectropomus moculafus, and 18 individuals of Plectropomus leopordus, with sequences
gg %. The most exploited species was Pledropomus
similarities ranges from g7 % up to
supported by analysis of the
leopordus. The result of molecular identification was also
with high bootstrap
phylogeny, which showed that all sequences form three different clades
supports.
Keyrrords: DNA barcoding grouper, South Sulawesi
bio.unsoed.ac.id
Barcoding of grouper species in Spermonde Archipelago,
Nuryanto, A.t, Pramono H'1, Kochzius M'2
1
lndonesia
Faculty Biology, Jenderal Soedirman University, Purwokerto'
zMarine Biology, Vrije Universiteit Brussel' Belgium
-
corresponding author: anuryanto2d)3@yahoo'com'
Abstract
marine live trade with high price' This
lndonesia,s groupers are marketed in the international
illegally fished specimens cannot be
leads to over exploitation due to illegal fishing. The
during sampling' in order to
identified properly due to time limitation provided by fisherman
is also known as DNA barcoding allows
avoid that the fish die. Molecular identification, which
is a prerequisite for
to identify any living organisms with high precision. such information
marker for molecular identification of
management. Cytocliro*" C oxidase I (COl) ii a standard
grouper species harvested in the spermonde
animars. The objective of the project is to identify
using the big-dye terminator
Archipelago. The amplified col fragments were sequenced
BioEdit (ver' 7'0'4'1)' A
method. All sequences were edited manually using the softeware
implemented in BioEdit (ver'
multiple sequences alignment was done using ClustalW as
available in the barcoding database
7.O. .tl.The orthologylo pr"uious published sequences
verified. To support this result' a
BOLD (http:/lboldsystems.org) and GenBank were also
programme MEGA (v' A'0) using teh
phylogenetic tree was reconstructed with the help of the
bootstrap replicates' A total of 28
neighbaur joining (NJ) method with 10fr) non-parametric
identification proved that only one
sequences were obtained during the study. Molecular
with Epinephelus ongus' The remaining
sequences matched well t99.8%) in the BOLD data base
and proved that the sequences
27 sequences were blasted to the data available in GenBank
genus Epinephelus consist of eight
were homologous with Epfne phelus and Plectropomus. The
consist of one individual of
individuals of Epinephelus ongus, while the genus Plectropomus
plectropomus moculafus, and 18 individuals of Plectropomus leopordus, with sequences
gg %. The most exploited species was Pledropomus
similarities ranges from g7 % up to
supported by analysis of the
leopordus. The result of molecular identification was also
with high bootstrap
phylogeny, which showed that all sequences form three different clades
supports.
Keyrrords: DNA barcoding grouper, South Sulawesi
bio.unsoed.ac.id