Bovine Serum Albumin (BSA) sebanyak 100 mg dilarutkan kedalam 100 ml ddH2O

7. LAMPIRAN

  Lampiran 1. Kurva Standar Protein

• Pembuatan Reagen Bradford

  

Commasive blue sebanyak 0,01 gram dilarutkan kedalam 50 ml etanol 95% dan

ditambah dengan asam fosfor 85% sebanyak 100 ml. Larutan yang digunakan

diencerkan dengan menggunakan ddH

2 O hingga 1000 ml. Larutan tersebut kemudian di saring dan disimpan dalam botol gelap pada suhu rendah (Bollag & Stuart, 1991).

• Pembuatan Kurva Standar Protein

  Bovine Serum Albumin (BSA) sebanyak 100 mg dilarutkan kedalam 100 ml ddH

  2 O

sehingga diperoleh larutan stok BSA 1 mg/ml. Selanjutnya dibuat seri pengenceran

larutan standar dengan konsentrasi 0 mg/ml; 0,1 mg/ml; 0,2 mg/ml; 0,3 mg/ml; 0,4

mg/ml; 0,5 mg/ml; 0,6 mg/ml; 0,7 mg/ml; 0,8 mg/ml; 0,9 mg/ml; dan 1 mg/ml. Dari

masing-masing konsentrasi diambil 0,1 ml larutan standar dan ditambahkan 5 ml larutan

Bradford. Kemudian didiamkan selama 30 menit dan diukur absorbansinya pada λ 595

nm (Bradford, 1976).

  Lampiran 2. Komposisi Reagen Analisis Molekuler Nama Larutan Komposisi

• 29,2 gram acrylamide Larutan A • 0,8 gram bis-acrylamide (Acrylamide Stock Solution), 100 ml 30% acrylamide, 0,8% bis-

  acrylamide Larutan B

• 75 ml 2M Tris-HCl (pH 8,8) --- 1,5 M (4x Separating Gel Buffer), 100 ml • 4 ml 10% SDS --- 0,4 %

• • 21 ml ddH

  2 O Larutan C

• 50 ml 1M Tris-HCl (pH 6,8) --- 0,5 M (4x Stacking Gel Buffer), 100 ml • 4 ml 10% SDS --- 0,4 %

• • 46 ml ddH

  2 O 10% Ammonium Persulphate

• 0,5 gram APS O (APS) • 5 ml DDH

  2 Buffer Elektroforesis

• 3 gr Tris --- 25Mm
• 14,4 gram glycine --- 192Mm
• 1 gram SDS --- 0,1%
• ddH

  2 O sampai 1 liter Sampel Buffer

• 3,028 gram Tris • 0,25 ml larutan C • 0,4 ml 10% SDS
• 25 ml gliserol
• 0,2 mg bromophenol blue
• 0,031 gram Dithiothreitol (DTT) Lampiran 3. Kurva Standar BSA (Bovine Serum Albumin) Low Molecular Weight

  1,000 y = -0,985x + 1,803 0,800 R² = 0,950 0,600

  Series1 0,400 Linea r (Series1) 0,200

  0,000 0,000 0,500 1,000 1,500 2,000

• 0,200

  

Lampiran 4. Berat Molekul Fraksi Protein Glutenin Adonan Tepung Terigu Protein

Tinggi yang Mengandung Kayu Manis Konsentrasi Rf Berat Molekul (kDa) 0% kayu manis (tanpa kayu manis) 0,04

  0,10 0,14 0,19 0,23

  62,65 54,81 50,19 44,79 40,78 1,5% kayu manis

  0,05 0,10 0,13 0,18 0,22 61,42

  54,04 50,62 45,30 41,36

  3% kayu manis 0,04 0,09 0,12 0,16 0,20

  63,01 55,92 51,64 47,14 43,29 Lampiran 5. Analisis Data Hasil Penelitian Adonan tepung terigu yang mengandung

  Kayu Manis hardness

  Duncan a

  6 207,6350 6 288,6367 6 363,6717

1,000 1,000 1,000

konsentrasi kontrol kayu manis 1,5% kayu manis 3% Sig.

  N

  1

  2

  3 Subset for alpha = .05 Means for groups in homogeneous subsets are displayed.

  Uses Harmonic Mean Sample Size = 6,000.

  a. Lampiran 6. Hasil Pengolahan SPSS Roti Kukus yang Mengandung Kayu Manis

• Analisis Tingkat Kekerasan (Hardness)
• Tanpa Penambahan Kayu Manis (Kontrol)

  hardness hardness_kontrol a,b

  Duncan Subset perlakuan N

  1

  2

  3 proofing 6 118,8150 mixing 6 180,1733 steaming 6 386,8167 Sig.

  1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 119,757.

  a.

  Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Penambahan Kayu Manis Konsentrasi 1,5%

  hardness_1,5% hardness a,b

  Duncan Subset perlakuan N

  1

  2 proofing 6 174,4100 mixing 6 234,3617 steaming 6 1225,8167 Sig.

  ,164 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 5046,095.

  a.

  Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Penambahan Kayu Manis Konsentrasi 3%

  hardness_3% hardness a,b

  Duncan Subset perlakuan N

  1

  2

  3 proofing 6 252,2850 mixing 6 358,7067 steaming 6 1614,6833

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 1549,066.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Tahapan Mixing

  hardness_mixing hardness a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 180,1733 kons 1,5% 6 234,3617 kons 3% 6 358,7067

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 84,606.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Tahapan Proofing

  hardness_proofing hardness a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 118,8150 kons 1,5% 6 174,4100 kons 3% 6 252,2850

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 631,594.

• Tahapan Steaming

  hardness hardness_steaming a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 386,8167 kons 1,5% 6 1225,8167 kons 3% 6 1614,6833

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 5998,717.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Analisis Warna • Tingkat Kecerahan/Lightness (L*)

  L a

  Duncan Subset for alpha = .05 konsentrasi N

  1

  2

  3 kons 3% 6 62,4533 kons 1,5% 6 66,8917 Kontrol 6 84,2567 Sig.

  1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed.

a. Uses Harmonic Mean Sample Size = 6,000.

• Nilai a*

  a a

  Duncan Subset for alpha = .05 konsentrasi N

  1

  2

  3 kontrol 6 -2,9100 kons 1,5% 6 5,1633 kons 3% 6 8,9817 Sig.

  1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 6,000.

• Nilai b*

  b a

  Duncan Subset for alpha = .05 konsentrasi N

  1

  2

  3 kontrol 6 21,0550 kons 1,5% 6 25,2833 kons 3% 6 28,1817 Sig.

  1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 6,000.

• Analisis Volume Pengembangan • Tanpa Penambahan Kayu Manis (Kontrol)

  volume volume_kontrol a,b

  Duncan Subset perlakuan N

  1

  2

  3 mixing 6 32,1687 proofing 6 50,1483 steaming 6 84,3860 Sig.

  1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 2,284.

  a.

  Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Penambahan Kayu Manis Konsentrasi 1,5%

  volume_1,5% volume a,b

  Duncan Subset perlakuan N

  1

  2

  3 mixing 6 31,3758 proofing 6 45,4053 steaming 6 69,9873 Sig.

  1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 3,883.

  a.

  Uses Harmonic Mean Sample Size = 6,000.

  b.

• Penambahan Kayu Manis 3%

  volume_3% volume a,b

  Duncan Subset perlakuan N

  1

  2

  3 mixing 6 31,1778 proofing 6 42,1075 steaming 6 63,7912

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 2,102.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Tahapan Mixing

  volume_mixing volume a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 32,1687 kons 1,5% 6 50,1483 kons 3% 6 84,3860

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 2,284.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Tahapan Proofing

  volume_proofing volume a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 31,3758 kons 1,5% 6 45,4053 kons 3% 6 69,9873

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 4,080.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Tahapan Steaming

  volume_steaming volume a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 31,1778 kons 1,5% 6 42,1075 kons 3% 6 63,7912

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 2,102.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Aktivitas Antioksidan • Tanpa Penambahan Kayu Manis (Kontrol)

  antioksidan_kontrol antioksidan a,b

  Duncan Subset perlakuan N

  1

  2 mixing 6 4,4567 steaming 6 6,8017 proofing 6 7,9583 Sig.

  1,000 ,051 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = ,888.

  a.

  Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Penambahan Kayu Manis Konsentrasi 1,5%

  antioksidan antioksidan_1,5% a,b,c

  Duncan Subset perlakuan N

  1

  2 mixing 5 20,0580 steaming 6 21,0150 21,0150 proofing 6 22,3033 Sig. ,272 ,146

  Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 1,967.

  a.

  Uses Harmonic Mean Sample Size = 5,625.

  b. The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.

  c.

  Alpha = ,05.

• Penambahan Kayu Manis 3%

  antioksidan_3% antioksidan a,b

  Duncan Subset perlakuan N

  1

  2 mixing 6 33,0867 steaming 6 33,9183 proofing 6 36,2167

  Sig. ,432 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 3,176.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Tahapan Mixing

  antioksidan_mixing antioksidan a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 4,4567 kons 1,5% 6 19,7500 kons 3% 6 33,0867

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 1,551.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.

• Tahapan Proofing

  antioksidan antioksidan_proofing a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 7,9583 kons 1,5% 6 22,3033 kons 3% 6 36,2167

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 2,335.

• Tahapan Steaming

  antioksidan antioksidan_steaming a,b

  Duncan Subset konsentrasi N

  1

  2

  3 kontrol 6 6,8017 kons 1,5% 6 21,0150 kons 3% 6 33,9183

  Sig. 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = 2,203.

  a. Uses Harmonic Mean Sample Size = 6,000.

  b.

  Alpha = ,05.