7. LAMPIRAN Lampiran 1. Hasil Pengujian Awal Susu Kacang Hijau - APLIKASI Lactobacillus plantarum EM1 DAN Lactobacillus pentosus EM1 SEBAGAI STARTER DALAM PEMBUATAN MINUMAN PROBIOTIK SUSU KACANG HIJAU - Unika Repository

7. LAMPIRAN Lampiran 1. Hasil Pengujian Awal Susu Kacang Hijau Analisa Hasil

  o Kandungan gula 8,13 Brix Nilai pH 6,75 Kandungan protein 2,81%

  Lampiran 2. Hasil Analisa Data dengan SPSS ƒ Hasil Analisa Data Berdasarkan Jenis Bakteri Asam Laktat

  PH_1 a

  Duncan Subset for alpha = .05 BAL N

  1

  2

  3 Lactobacillus bulgaricus 3 4.0333 Lactobacillus pentosus 3 4.0567 Lactobacillus plantarum

  

3 4.4467

Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  GULA_1 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2 Lactobacillus bulgaricus 3 6.8000 Lactobacillus pentosus 3 6.9333 Lactobacillus plantarum 3 7.2667 Sig. .071 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  ASAM_1 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2

  3 Lactobacillus plantarum 3 .2700 Lactobacillus pentosus 3 .3750 Lactobacillus bulgaricus 3 .4650 Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  PH_10 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2

  3 Lactobacillus pentosus 3 3.9500 Lactobacillus bulgaricus 3 4.0100 Lactobacillus plantarum 3 4.3533 Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  GULA_10 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2 Lactobacillus pentosus 3 6.2000 Lactobacillus bulgaricus 3 6.3333 Lactobacillus plantarum 3 7.0000 Sig.

  .267 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  ASAM_10 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2

  3 Lactobacillus plantarum 3 .3450 Lactobacillus pentosus 3 .4650 Lactobacillus bulgaricus 3 .6450 Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  PH_20 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2

  3 Lactobacillus pentosus 3 3.9100 Lactobacillus bulgaricus 3 4.0567 Lactobacillus plantarum 3 4.5900 Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  GULA_20 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2 Lactobacillus pentosus 3 6.0667 Lactobacillus bulgaricus 3 7.0000 Lactobacillus plantarum 3 7.1333 Sig.

  1.000 .134 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  ASAM_20 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2

  3 Lactobacillus plantarum 3 .2700 Lactobacillus bulgaricus 3 .3750 Lactobacillus pentosus 3 .5250 Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  PH_30 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2

  3 Lactobacillus pentosus 3 3.86000 Lactobacillus bulgaricus 3 4.04000 Lactobacillus plantarum 3 4.50033 Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  GULA_30 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2 Lactobacillus pentosus 3 5.8000 Lactobacillus bulgaricus 3 6.4000 Lactobacillus plantarum 3 6.7333 Sig.

  1.000 .060 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  ASAM_30 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2

  3 Lactobacillus plantarum 3 .3600 Lactobacillus bulgaricus 3 .4500 Lactobacillus pentosus 3 .5250 Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  PH_40 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2

  3 Lactobacillus pentosus 3 3.86000 Lactobacillus bulgaricus 3 4.03000 Lactobacillus plantarum 3 4.20033 Sig.

  1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  GULA_40 a

  Duncan

Subset for alpha = .05

BAL N

  1

  

2

  3 Lactobacillus pentosus 3 5.8667 Lactobacillus bulgaricus 3 6.1333 Lactobacillus plantarum 3 6.6333 Sig. 1.000 1.000 1.000

  Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  ASAM_40 a

  Duncan Subset for alpha = .05

  BAL N

  1

  2 Lactobacillus plantarum 3 .45000 Lactobacillus bulgaricus 3 .54000 Lactobacillus pentosus 3 .54033 Sig.

  1.000 .267 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  PROT_1 a

  Duncan Subset for alpha

  = .05 BAL N

  1 Lactobacillus bulgaricus 2 2.9600 Lactobacillus plantarum 2 3.0000 Lactobacillus pentosus 2 3.0200 Sig.

  .723 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 2.000.

  PROT_40 a

  Duncan Subset for alpha

  = .05 BAL N

  1 Lactobacillus bulgaricus 2 2.7250 Lactobacillus plantarum 2 2.8000 Lactobacillus pentosus 2 2.8350 Sig. .173 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 2.000.

  ƒ Hasil Analisa Data Berdasarkan Hari Penyimpanan

PH_BUL

  a Duncan Subset for alpha = .05 HARI N

  1

  2

  3

  4 hari ke-10 3 4.0100 hari ke-40 3 4.0300 hari ke-1 3 4.0333 hari ke-30 3 4.0400 hari ke-20 3 4.0567 Sig.

  1.000 .290 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

GULA_BUL

  a Duncan Subset for alpha = .05 HARI N

  1

  2

  3 hari ke-40 3 6.1333 hari ke-10 3 6.3333 6.3333 hari ke-30 3 6.4000 hari ke-1 3 6.8000 hari ke-20 3 7.0000 Sig. .060 .496 .060

  Means for groups in homogeneous subsets are displayed.

  a.

ASAM_BUL

  a Duncan Subset for alpha = .05 HARI N

  1

  2

  3

  4 hari ke-20 3 .3900 hari ke-30 3 .4500 .4500 hari ke-1 3 .4800 .4800 hari ke-40 3 .5400 hari ke-10 3 .6600 Sig. .098 .383 .098 1.000

  Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

PH_PLAN

  a Duncan Subset for alpha = .05 HARI N

  1

  2

  3

  4

  5 hari ke-40 3 4.2000 hari ke-10 3 4.3533 hari ke-1 3 4.4467 hari ke-30 3 4.5000 hari ke-20 3 4.5900 Sig.

  1.000 1.000 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

GULA_PLA

  a

  Duncan Subset for alpha = .05

  HARI N

  1

  2

  3 hari ke-40 3 6.6333 hari ke-30 3 6.7333 hari ke-10 3 7.0000 hari ke-20 3 7.1333 7.1333 hari ke-1 3 7.2667 Sig.

  .165 .073 .073 Means for groups in homogeneous subsets are displayed.

  a.

  Uses Harmonic Mean Sample Size = 3.000.

ASAM_PLA

  a

  Duncan Subset for alpha = .05

  HARI N

  1

  2

  3 hari ke-1 3 .2700 hari ke-20 3 .2700 hari ke-10 3 .3300 hari ke-30 3 .3600 hari ke-40 3 .4500 Sig.

  1.000 .145 1.000 Means for groups in homogeneous subsets are displayed.

PH_PEN

  Duncan

  a

GULA_PEN

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  2 Subset for alpha = .05 Means for groups in homogeneous subsets are displayed.

  1

  N

  1.000 .171 HARI hari ke-1 hari ke-10 hari ke-20 hari ke-30 hari ke-40 Sig.

  3 .3900 3 .4800 3 .5100 3 .5100 3 .5400

  a

  Duncan

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  2

  1

  N

  HARI hari ke-30 hari ke-40 hari ke-20 hari ke-10 hari ke-1 Sig.

  3 5.8000 3 5.8667 3 6.0667 6.0667 3 6.2000 3 6.9333 .071 .317 1.000

  Duncan a

  a.

  Uses Harmonic Mean Sample Size = 3.000.

  4 Subset for alpha = .05 Means for groups in homogeneous subsets are displayed.

  3

  2

  1

  N

  1.000 1.000 1.000 1.000 HARI hari ke-30 hari ke-40 hari ke-20 hari ke-10 hari ke-1 Sig.

  3 3.8600 3 3.8600 3 3.9100 3 3.9500 3 4.0567

3 Subset for alpha = .05 Means for groups in homogeneous subsets are displayed.

ASAM_PEN

  Lampiran 3. Syarat Mutu Minuman Susu Fermentasi Menurut SNI Persyaratan Tanpa perlakuan Dengan perlakuan panas setelah panas setelah No Kriteria Uji Satuan fermentasi fermentasi Tanpa Tanpa Normal Normal Lemak Lemak

1 Keadaan :

• 1.1 Penampakan cair cair
• 1.2 Bau normal/khas normal/khas

  1.3 Rasa

• asam/khas asam/khas

  1.4 Homogenitas - homogen homogen

  2 Lemak % min 0,6 maks 0,5 min 0,6 maks 0,5

  3 Padatan susu tanpa % min 3,0 min 3,0 lemak

  4 Protein % min 1,0 min 1,0

  5 Abu % maks 1,0 maks 1,0

  6 Keasaman tertitrasi % 0,2 s.d 0,9 0,2 s.d 0,9 (dihitung sebagai asam laktat)

  7 Cemaran logam :

  7.1 Timbal (Pb) mg/kg maks 0,02 maks 0,02

  7.2 Merkuri (Hg) mg/kg maks 0,03 maks 0,03

  8 Cemaran arsen mg/kg maks 0,1 maks 0,1 (As)

9 Cemaran mkroba :

  9.1 Bakteri coliform APM/m maks 10 maks 10 l

  

9.2 Salmonella sp / - negatif negatif

25ml

  

9.3 Listeria - negatif negatif

monocytogenes / 25ml

  6

• 10 Kultur starter Koloni/ min. 1 x 10 ml