IM Tahap maturasi Warna gonad 1

  Lampiran 1.

  2.2 Spawning Kuning

  2.6 Spawning Merah muda

  14 Pa.2.25 343.033 3.333 Spawning Merah muda Rerata 396.167 SD 239.388 No. Kode individu Diameter tubulus (µm)

  IM Tahap maturasi Warna gonad

  1 Pa.2.02 400.961

  1.4 Spermatogenesis Kuning

  2 Pa.2.03 437.486

  1 Spermatogenesis Kuning

  3 Pa.2.04 508.041

  5 Late post spawning Kuning

  4 Pa.2.06 421.378

  5 Pa.2.08 550.449

  4.2 Early post spawning Kuning

  1.4 Spermatogenesis Kuning

  6 Pa.2.11 240.187

  5 Late post spawning Kuning

  7 Pa.2.14 374.593

  1 Spermatogenesis Kuning

  8 Pa.2.19 306.774

  1 Spermatogenesis Merah muda

  9 Pa.2.21 495.327

  1 Spermatogenesis Kuning

  10 Pa.2.24 248.817

  5 Late post spawning Kuning

  13 Pa.2.23 360.177

  12 Pa.2.22 266.452

  Data pengukuran diameter tubulus serta penentuan indeks maturitas (IM) P. australis jantan dan betina dari Pantai Timur Surabaya pada bulan Februari 2012 Jantan

  5 Pa.2.12 276.542

  Betina No.

  Kode individu Diameter tubulus (µm)

  IM Tahap maturasi Warna gonad

  1 Pa.2.01 273.121

  4 Early post spawning Kuning

  2 Pa.2.05 351.302

  3.6 Early post spawning Merah muda

  3 Pa.2.07 398.909

  2.6 Spawning Merah muda

  4 Pa.2.10 1164.613

  2.6 Spawning Merah muda

  5 Late post spawning Kuning

  4.75 Late post spawning Kuning

  6 Pa.2.13 359.074

  3 Spawning Merah muda

  7 Pa.2.15 348.404

  3.4 Spawning Merah muda

  8 Pa.2.16 595.285

  3.8 Early post spawning Oranye

  9 Pa.2.17 252.909

  4 Early post spawning Merah muda

  10 Pa.2.18 350.482

  4.2 Early post spawning Merah muda

  11 Pa.2.20 206.036

  Rerata 398.401 SD 106.961

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  Lampiran 2 . Data pengukuran diameter tubulus serta penentuan indeks maturitas (IM) P. australis jantan dan betina dari Pantai Timur Surabaya pada bulan Maret 2012 Jantan

  8 Pa.3.18 642.521

  3 Pa.3.09 600.518

  5 Late post spawning Oranye

  4 Pa.3.12 449.023

  3.6 Early post spawning Oranye

  5 Pa.3.13 687.317

  3.2 Spawning Merah muda

  6 Pa.3.16 593.577

  4 Early post spawning Merah muda

  7 Pa.3.17 512.282

  5 Late post spawning Oranye

  3.8 Early post spawning Merah muda

  2 Pa.3.08 479.954

  9 Pa.3.19 797.972

  3 Spawning Merah muda

  10 Pa.2.20 343.505

  5 Late post spawning Kuning

  11 Pa.3.23 547.123

  5 Late post spawning Oranye

  12 Pa.3.24 434.885

  2.8 Spawning Merah muda

  13 Pa.3.25 907.907

  2.4 Mature Merah muda

  5 Late post spawning Oranye

  5 Late post spawning Oranye

  No. Kode individu Diameter tubulus (µm) IM Tahap maturasi Warna gonad

  6 Pa.3.07 484.375

  1 Pa.3.01 353.970

  5 Late post spawning Oranye

  2 Pa.3.02 570.559

  4.2 Early post spawning Oranye

  3 Pa.3.03 781.812

  2.6 Spawning Putih

  4 Pa.3.04 479.219

  2.8 Spawning Oranye

  5 Pa.3.06 588.308

  2.6 Spawning Oranye

  4.2 Early post spawning Oranye

  1 Pa.3.05 412.452

  7 Pa.3.10 374.080

  4.8 Late post spawning Oranye

  8 Pa.3.14 451.235

  1.2 Mature Putih

  9 Pa.3.15 830.034

  1.4 Spermatogenesis Putih

  10 Pa.3.21 684.958

  2.4 Mature Putih

  Rerata 559.855 SD 154.834 Betina No.

  Kode individu Diameter tubulus (µm)

  IM Tahap maturasi Warna gonad

  Rerata 569.926 SD 153.833

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  Lampiran 3 . Data pengukuran diameter tubulus serta penentuan indeks maturitas (IM) P. australis jantan dari Pantai Timur Surabaya pada bulan

  9 Pa.4.20 366.795

  4 Pa.4.09 618.575

  3 Spawning Merah muda

  5 Pa.4.11 537.094

  3.4 Spawning Merah muda

  6 Pa.4.12 468.010

  5 Late post spawning Oranye

  7 Pa.4.15 350.741

  4 Early post spawning Oranye

  8 Pa.4.16 408.263

  4 Early post spawning Merah muda

  4 Early post spawning Oranye

  3 Pa.4.05 712.740

  10 Pa.4.21 698.240

  3.2 Spawning Merah muda

  11 Pa.4.22 1173.431

  5 Late post spawning Oranye

  12 Pa.4.23 372.502

  4 Early post spawning Oranye

  13 Pa.4.24 366.352

  4.4 Early post spawning Oranye

  14 Pa.4.25 461.166

  5 Late post spawning Oranye

  5 Late post spawning Oranye

  4 Early post spawning Merah muda

  April 2012 Jantan No. Kode individu Diameter tubulus (µm)

  3.8 Early post spawning Putih

  IM Tahap maturasi Warna gonad

  1 Pa.4.02 886.977

  2.4 Mature Kuning

  2 Pa.4.04 641.250

  3.4 Spawning Kuning

  3 Pa.4.07 743.209

  3.5 Spawning Putih

  4 Pa.4.08 303.286

  3.8 Early post spawning Putih

  5 Pa.4.13 506.001

  6 Pa.4.14 570.764

  2 Pa.4.03 609.894

  2.8 Spawning Putih

  7 Pa.4.18 439.385

  2.33 Mature Putih

  8 Pa.4.19 547.781

  3 Spawning Putih

  Rerata 579.831 SD 180.304 Betina

  No . Kode individu Diameter tubulus (µm)

  IM Tahap maturasi Warna gonad

  1 Pa.4.01 750.305

  3.67 Early post spawning Oranye

  Rerata 563.865 SD 224.674

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  Lampiran 4.

  Data pengelompokan rerata diameter tubulus berdasarkan tahap perkembangan gonad P. australis jantan dan betina Jantan

Diameter tubulus setiap tahap perkembangan gonad (µm)

  Spermatoge nesis

Mature Spawning Early

Post

  Spawning Late Post Spawning

  Rerata 403.545 606.286 596.715 466.055 354.019 SD 81.197 196.729 122.703 114.522 109.254 Betina

Diameter tubulus setiap tahap perkembangan gonad (µm)

  Oogenesis

Mature Spawning Early

Post

  Spawning Late Post Spawning

  Rerata 0 907.907 562.358 437.467 516.498 SD 247.744 151.094 260.679

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  Lampiran 5.

  Grafik suhu udara di Pantai Timur Surabaya bulan Februari, Maret, dan April 2012 Februari

  Maret ^{25.5 26 26.5 27 27.5 28 28.5 29 29.5 30 30.5 5 10}

¹⁵

^{20 25 30 35} S u h u (˚

  26.5 ^{27 27.5 28 28.5 29 29.5 30 30.5} _{5 10 15 20 25 30 35} Su

  hu (˚

  

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C) Hari

C) Hari

  

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April ₃₁

  30.5 ₃₀ C)

   (˚ _29.5 u h u S _{28.5 29}

  28 _{5 10 15 20 25 30 35} Hari

  

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Lampiran 6 Uji normalitas data sampel P.australis dari Pantai Timur Surabaya

  Diameter Tubulus N

  75 a,,b

  Normal Parameters Mean 502.0589 Std. Deviation 197.17801 Most Extreme Absolute .096 Differences

  Positive .096 Negative -.079 Kolmogorov-Smirnov Z .829 Asymp. Sig. (2-tailed) .498 a. Test distribution is Normal.

  b. Calculated from data. diameter_jantan N

  293 a,,b

  Normal Parameters Mean 499.8432 Std. Deviation 213.17253 Most Extreme Differences Absolute .076 Positive .076

  Negative -.052 Kolmogorov-Smirnov Z 1.295 Asymp. Sig. (2-tailed) .070 a. Test distribution is Normal.

  b. Calculated from data.

  

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D.tb_betina N

  41 a,,b

  Normal Parameters Mean 508.5240

Std. Deviation 222.34376

Most Extreme Differences Absolute .136

Positive .136

  

Negative -.106

Kolmogorov-Smirnov Z .874 Asymp. Sig. (2-tailed) .430 a. Test distribution is Normal.

  b. Calculated from data. diameter_betina N

  333 a,,b

  Normal Parameters Mean 508.3463 Std. Deviation 227.86545 Most Extreme Differences Absolute .083 Positive .083

  Negative -.063 Kolmogorov-Smirnov Z 1.521 Asymp. Sig. (2-tailed) .020 a. Test distribution is Normal.

  b. Calculated from data.

  Lampiran 7 Uji beda indeks maturitas P. australis pada bulan Februari, Maret, dan April 2012 Kruskal-Wallis Test Ranks

  Bulan pengambilan

sampel N Mean Rank

Indeks Maturitas Februari

  25

  34.72 Maret

  25

  40.18 April

  25

  39.10 Total

  75 Test Statistics a,b

  Indeks Maturitas Chi-Square .890 df

2 Asymp. Sig. .641

a. Kruskal Wallis Test

  b. Grouping Variable: Bulan pengambilan sampel

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  Lampiran 8

Uji beda indeks maturitas P. australis jantan pada bulan Februari, Maret, dan

April 2012 Kruskal-Wallis Test Ranks

  Bulan pengambila n sampel N Mean Rank

  IM_Jantan Februari

  25

  14.54 Maret

  3

  14.17 Total

28 Test Statistics

  a,b

  IM_Jantan Chi-Square .006 df

1 Asymp. Sig. .941

a. Kruskal Wallis Test

  b. Grouping Variable: Bulan pengambilan sampel

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  Lampiran 9

Uji beda indeks maturitas P. australis betina pada bulan Februari, Maret, dan

April 2012 Kruskal-Wallis Test Ranks

  Bulan pengambila n sampel N Mean Rank

  IM_Betina Februari

  25

  20.98 Maret

  16

  21.03 Total

41 Test Statistics

  a,b

  IM_Betina Chi-Square .000 df

1 Asymp. Sig. .989

a. Kruskal Wallis Test

  b. Grouping Variable: Bulan pengambilan sampel

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Lampiran 10 Analisis hubungan diameter tubulus gonad P. australis jantan dan betina terhadap indeks maturitas Nonparametric Correlations Correlations diameter_betina ind.m.betina

• Spearman's rho diameter_betina Correlation Coefficient 1.000 -.113 Sig. (2-tailed) . .040 N 333 333
• ind.m.betina Correlation Coefficient -.113 1.000 Sig. (2-tailed) .040 . N 333 333 *. Correlation is significant at the 0.05 level (2-tailed).

  Correlations diameter_jantan ind.m.jantan

• Spearman's rho diameter_jantan Correlation Coefficient 1.000 -.149 Sig. (2-tailed) . .011 N 293 293
• ind.m.jantan Correlation Coefficient -.149 1.000 Sig. (2-tailed) .011 . N 293 293 *. Correlation is significant at the 0.05 level (2-tailed).

  

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  Lampiran 13.

  Dokumentasi penelitian

Timbangan digital Botol processing

Paraffin bath

  Mikrotom Staining jar Sampel P. australis

  

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