Uji In vitro Aktivitas Antelmintik Ekstrak Etilasetat Daun Pugun Tanoh [Curanga fel-terrae (Lour.) Merr.]
Lampiran 1. Hasil identifikasi tanaman
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Lampiran 2. Hasil identifikasi hewan percobaan
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Lampiran 3. Surat keterangan baku obat
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Lampiran 4. Gambar tumbuhan dan daun pugun tanoh
Tumbuhan pugun tanoh
Daun pugun tanoh segar
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Lampiran 5. Gambar hewan percobaan
Cacing tanah Pheretima posthuma
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Lampiran 6. Gambar simplisia dan serbuk simplisia daun pugun tanoh
Simplisia daun pugun tanoh
Serbuk simplisia daun pugun tanoh
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Lampiran 7. Gambar mikroskopik simplisia daun pugun tanoh dengan
perbesaran 10 x 40
a
b
c
a
d
e
f
g
h
i
Keterangan:
a. Berkas pembuluh angkut bentuk spiral
b. Rambut kelenjar
c. Epidermis
d. Tulang daun
e. Rambut multiseluler
f. Kristal Ca Oksalat bentuk prisma
g. Stomata tipe anomositik
h. Stomata tipe diasitik
i. Sel tetangga
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Lampiran 8. Ekstrak etilasetat daun pugun tanoh
Ekstrak etilasetat daun pugun tanoh
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Lampiran 9. Perhitungan rendemen simplisia dan ekstrak
Berat basah
2100 g
Berat simplisia
600 g
% Rendemen simplisia =
erat sim lisia
x 100%
erat basa
=
x 100% = 28,57%
% Rendemen ekstrak =
erat ekstrak
x 100%
erat sim lisia
=
,
Berat ekstrak
16, 09 g
x 100% = 2,68%
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Lampiran 10. Perhitungan kadar air simplisia
% Kadar air =
No.
1
2
3
olume air (ml)
erat sam el ( )
Berat sampel (g)
5,021
5,010
5, 021
% Kadar air =
Volume awal (ml)
1,80
2,00
2,20
olume ak ir- olume a al
erat sam el
1. % Kadar air =
2. % Kadar air =
3. % Kadar air =
,
,
,
x 100% = 3,99%
,
,
,
x 100% = 3,98%
,
% rata-rata kadar air simplisia =
x 100%
x 100% = 3,98%
,
,
Volume akhir (ml)
2,00
2,20
2,40
,
,
,
= 3,98%
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Lampiran 11. Perhitungan kadar sari larut air simplisia
% Kadar sari larut dalam air =
No.
1.
2.
3.
berat sari ( )
berat sam el ( )
x
x 100%
Berat sampel (g)
5,025
5,045
5,078
1. % Kadar sari larut dalam air =
2. % Kadar sari larut dalam air =
3. % Kadar sari larut dalam air =
% rata-rata kadar sari larut dalam air =
Berat sari (g)
0,203
0,210
0,199
,
,
,
,
,
,
,
x
x 100% = 20,19%
x
x 100% = 20,81%
x
x 100% = 19,59%
,
,
= 20,20%
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Lampiran 12. Perhitungan kadar sari larut etanol simplisia
% Kadar sari larut dalam etanol =
No.
1.
2.
3.
berat sari ( )
berat sam el ( )
x
x 100%
Berat sampel (g)
5,010
5,017
5,007
Berat sari (g)
0,226
0,212
0,198
1. % Kadar sari larut dalam etanol =
2. % Kadar sari larut dalam etanol =
3. % Kadar sari larut dalam etanol =
% rata-rata kadar sari larut dalam etanol =
,
,
,
,
,
,
x
x 100% = 22,55%
x
x 100% = 21,12%
x
x 100% = 19,77%
,
,
= 21,15%
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Lampiran 13. Perhitungan kadar abu total simplisia
% Kadar abu total =
No.
1.
2.
3.
berat abu ( )
berat sam el ( )
x 100%
Berat sampel (g)
2,015
2,030
2,024
1. % Kadar abu total =
2. % Kadar abu total =
3. % Kadar abu total =
% rata-rata kadar abu total =
,
Berat abu (g)
0,185
0,180
0,182
x 100% = 9,18%
,
,
x 100% = 8,87%
,
,
x 100% = 8,99%
,
,
,
,
= 9,01%
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Lampiran 14. Perhitungan kadar abu tidak larut asam simplisia
% Kadar abu tidak larut asam =
No.
1.
2.
3.
berat abu ( )
berat sam el ( )
x 100%
Berat sampel (g)
2,015
2,030
2,024
1. % Kadar abu tidak larut asam =
2. % Kadar abu tidak larut asam =
3. % Kadar abu tidak larut asam =
% rata-rata kadar abu tidak larut asam =
Berat abu (g)
0,019
0,020
0,017
,
x 100% = 0,94%
,
,
x 100% = 0,99%
,
,
x 100% = 0,84%
,
,
,
,
= 0,92%
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Lampiran 15. Perhitungan kadar air ekstrak etilasetat daun pugun tanoh
% Kadar air =
No.
1
2
3
olume air (ml)
erat sam el ( )
x 100%
Berat sampel (g)
5,040
5,040
5,050
1. % Kadar air =
2. % Kadar air =
3. % Kadar air =
,
- ,
,
,
- ,
,
,
- ,
,
Volume awal (ml)
2,35
2,50
2,75
Volume akhir (ml)
2,50
2,75
2,90
x 100% = 2,98%
x 100% = 2,98%
x 100% = 2,97%
% rata-rata kadar air ekstrak =
,
,
,
= 2,98%
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Lampiran 16. Perhitungan kadar abu total ekstrak etilasetat daun pugun tanoh
% Kadar abu total =
No.
1.
2.
3.
berat abu ( )
berat sam el ( )
Berat sampel (g)
2,002
2,030
2,024
1. % Kadar abu total =
2. % Kadar abu total =
3. % Kadar abu total =
,
,
,
,
,
,
% rata-rata kadar abu total =
x 100%
Berat abu (g)
0,077
0,081
0,070
x 100% = 3,86%
x 100% = 3,99%
x 100% = 3,46%
,
,
,
= 3,77%
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Lampiran 17. Perhitungan kadar abu tidak larut asam ekstrak etilasetat daun
pugun tanoh
% Kadar abu total =
No.
1.
2.
3.
berat abu ( )
berat sam el ( )
x 100%
Berat sampel (g)
2,002
2,030
2,024
1. % Kadar abu tidak larut asam =
2. % Kadar abu tidak larut asam =
3. % Kadar abu tidak larut asam =
Berat abu (g)
0,007
0,008
0,007
,
,
,
,
,
,
% rata-rata kadar abu tidak larut asam =
x 100% = 0,35%
x 100% = 0,39%
x 100% = 0,35%
,
,
,
= 0,36%
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Lampiran 18. Hasil uji orientasi pelarut
Konsentrasi Tween
80 (%)
0,5
1
1,5
Pengamatan Terhadap Cacing Pheretima posthuma
Kondisi
Waktu Kematian (menit)
Hidup
Hidup
Mati
284±2,82
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Lampiran 19. Uji aktivitas antelmintik
(1)
(2)
(3)
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(4)
(5)
(6)
(7)
(8)
(9)
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(10)
(11)
(12)
Keterangan :
1. Suspensi ekstrak etilasetat daun pugun tanoh
2. Kontrol negatif dengan larutan NaCl 0,9%
3. Kontrol pelarut dengan Tween 80 1%
4. Pemberian suspensi EEDPT kons. 5 mg/ml
5. Perubahan morfologi cacing
6. Pemberian suspensi EEDPT kons. 10 mg/ml
7. Perubahan morfologi cacing
8. Pemberian suspensi EEDPT kons.15 mg/ml
9. Perubahan morfologi cacing
10. Pemberian suspensi EEDPT kons. 20 mg/ml
11. Perubahan morfologi cacing
12. Kontrol positif dengan suspensi albendazole kons. 20 mg/ml
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Lampiran 20. Waktu paralisis cacing
Perlakuan
Pengujian (menit)
I
II
Larutan NaCl 0,9% (kontrol negatif)
‾
‾
Tween 80 1% (kontrol pelarut)
‾
‾
EEDPT 5 mg/ml
67
65
EEDPT 10 mg/ml
67
71
EEDPT 20 mg/ml
37
37
EEDPT 30 mg/ml
30
24
Albendazole 20 mg /ml (kontrol positif)
78
73
Keterangan : EEDPT = Ekstrak Etilasetat Daun Pugun tanoh
III
‾
‾
70
73
42
25
79
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Lampiran 21. Waktu kematian cacing
Perlakuan
Pengujian (menit)
I
II
Larutan NaCl 0,9% (kontrol negatif)
‾
‾
Tween 80 1% (kontrol pelarut)
‾
‾
EEDPT 5 mg/ml
181
187
EEDPT 10 mg/ml
107
95
EEDPT 20 mg/ml
47
45
EEDPT 30 mg/ml
36
31
Albendazole 20 mg /ml (kontrol positif)
185
190
Keterangan : EEDPT = Ekstrak Etilasetat Daun Pugun tanoh
III
‾
‾
195
102
59
34
182
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Lampiran 22. Uji statistika waktu paralisis cacing
Case Processing Summary
Waktu paralisis
Konsentrasi
EEDPT 5 mg/ml
EEDPT 10 mg/ml
EEDPT 20 mg/ml
EEDPT 30 mg/ml
Albendazole 20 mg/ml
N
3
3
3
3
3
Valid
Percent
100.0%
100.0%
100.0%
100.0%
100.0%
Cases
Missing
N Percent
0 0.0%
0 0.0%
0 0.0%
0 0.0%
0 0.0%
N
3
3
3
3
3
Total
Percent
100.0%
100.0%
100.0%
100.0%
100.0%
Descriptives
Konsentrasi
Waktu paralisis EEDPT 5
mg/ml
EEDPT 10
mg/ml
Mean
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Std.
Statistic Error
67.3333 1.45297
61.0817
73.5849
.
67.0000
6.333
2.51661
65.00
70.00
5.00
.
.586
1.225
.
.
70.3333 1.76383
62.7442
77.9225
.
71.0000
9.333
3.05505
67.00
73.00
6.00
.
-.935
.
1.225
.
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EEDPT 20
mg/ml
Mean
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
EEDPT 30
mg/ml
Mean
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Albendazole 20 Mean
mg/ml
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
38.6667 1.66667
31.4956
45.8378
.
37.0000
8.333
2.88675
37.00
42.00
5.00
.
1.732
.
1.225
.
26.3333 1.85592
18.3479
34.3187
.
25.0000
10.333
3.21455
24.00
30.00
6.00
.
1.545
1.225
.
.
76.6667 1.85592
68.6813
84.6521
.
78.0000
10.333
3.21455
73.00
79.00
6.00
.
-1.545
.
1.225
.
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Test of Normality
Kolmogorov-Smirnova
Shapiro-Wilk
Konsentrasi
Statistic
df Sig. Statistic df Sig.
Waktu paralisis EEDPT 5 mg/ml
.219 3
.
.987 3 .780
EEDPT 10 mg/ml
.253 3
.
.964 3 .637
EEDPT 20 mg/ml
.385 3
.
.750 3 .000
EEDPT 30 mg/ml
.328 3
.
.871 3 .298
Albendazole 20 mg/ml
.328 3
.
.871 3 .298
a. Lilliefors Significance Correction
Kruskall-Wallis Test
Ranks
Waktu Paralisis
Konsentrasi
EEDPT 5 mg/ml
EEDPT 10 mg/ml
EEDPT 20 mg/ml
EEDPT 30 mg/ml
Total
N
Mean Rank
3
3
3
3
12
8.50
10.50
5.00
2.00
Test Statistics
Waktu_Paralisi
s
Chi-Square
9.877
df
3
Asymp. Sig.
.020
a. Kruskal Wallis Test
b. Grouping Variable:
Konsentrasi
Multiple Comparisons
Dependent Variable: Waktuparalisis
Tukey HSD
(I) Konsentrasi
EEDPT 5
mg/ml
(J) Konsentrasi
EEDPT 10
mg/ml
EEDPT 20
mg/ml
EEDPT 30
mg/ml
Albendazole 20
mg/ml
Mean
Difference (I-J)
Std.
Error
Sig.
95% Confidence
Interval
Lower
Upper
Bound
Bound
-3.00000 2.44040 .736 -11.0316
5.0316
28.66667* 2.44040 .000
20.6351
36.6982
41.00000* 2.44040 .000
32.9684
49.0316
-9.33333* 2.44040 .022 -17.3649
-1.3018
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EEDPT 10
mg/ml
EEDPT 5
3.00000 2.44040
mg/ml
EEDPT 20
31.66667* 2.44040
mg/ml
EEDPT 30
44.00000* 2.44040
mg/ml
Albendazole 20
-6.33333 2.44040
mg/ml
EEDPT 20
EEDPT 5
-28.66667* 2.44040
mg/ml
mg/ml
EEDPT 10
-31.66667* 2.44040
mg/ml
EEDPT 30
12.33333* 2.44040
mg/ml
Albendazole 20
-38.00000* 2.44040
mg/ml
EEDPT 30
EEDPT 5
-41.00000* 2.44040
mg/ml
mg/ml
EEDPT 10
-44.00000* 2.44040
mg/ml
EEDPT 20
-12.33333* 2.44040
mg/ml
Albendazole 20
-50.33333* 2.44040
mg/ml
Albendazole 20 EEDPT 5
9.33333* 2.44040
mg/ml
mg/ml
EEDPT 10
6.33333 2.44040
mg/ml
EEDPT 20
38.00000* 2.44040
mg/ml
EEDPT 30
50.33333* 2.44040
mg/ml
*. The mean difference is significant at the 0.05 level.
.736
-5.0316
11.0316
.000
23.6351
39.6982
.000
35.9684
52.0316
.145 -14.3649
1.6982
.000 -36.6982 -20.6351
.000 -39.6982 -23.6351
.004
4.3018
20.3649
.000 -46.0316 -29.9684
.000 -49.0316 -32.9684
.000 -52.0316 -35.9684
.004 -20.3649
-4.3018
.000 -58.3649 -42.3018
.022
1.3018
17.3649
.145
-1.6982
14.3649
.000
29.9684
46.0316
.000
42.3018
58.3649
Paralysis Time
Tukey HSDa
Subset for alpha = 0.05
2
3
Konsentrasi
N
1
EEDPT 30 mg/ml
3
26.3333
EEDPT 20 mg/ml
3
38.6667
EEDPT 5 mg/ml
3
EEDPT 10 mg/ml
3
Albendazole 20 mg/ml
3
Sig.
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
67.3333
70.3333
.736
4
70.3333
76.6667
.145
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Lampiran 23. Uji statistika waktu kematian cacing
Case Processing Summary
Waktu Kematian
Konsentrasi
EEDPT 5 mg/ml
EEDPT 10 mg/ml
EEDPT 20 mg/ml
EEDPT 30 mg/ml
Albendazole 20 mg/ml
N
3
3
3
3
3
Valid
Percent
100.0%
100.0%
100.0%
100.0%
100.0%
Cases
Missing
N Percent
0
0.0%
0
0.0%
0
0.0%
0
0.0%
0
0.0%
N
3
3
3
3
3
Total
Percent
100.0%
100.0%
100.0%
100.0%
100.0%
Descriptives
Konsentrasi
Waktu Kematian EEDPT 5
mg/ml
EEDPT 10
mg/ml
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Std.
Statistic Error
187.6667 4.05518
Lower
Bound
Upper
Bound
170.2187
205.1147
.
187.0000
49.333
7.02377
181.00
195.00
14.00
.
.423
1.225
.
.
101.3333 3.48010
Lower
Bound
Upper
Bound
86.3597
116.3070
.
102.0000
36.333
6.02771
95.00
107.00
12.00
.
-.492
.
1.225
.
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EEDPT 20
mg/ml
EEDPT 30
mg/ml
Albendazole
20 mg/ml
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
50.3333 4.37163
Lower
Bound
Upper
Bound
31.5237
69.1429
.
47.0000
57.333
7.57188
45.00
59.00
14.00
.
1.597
1.225
.
.
33.6667 1.45297
Lower
Bound
Upper
Bound
27.4151
39.9183
.
34.0000
6.333
2.51661
31.00
36.00
5.00
.
-.586
1.225
.
.
185.6667 2.33333
Lower
Bound
Upper
Bound
175.6271
195.7062
.
185.0000
16.333
4.04145
182.00
190.00
8.00
.
.722
.
1.225
.
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Normality Test
Kolmogorov-Smirnova
Shapiro-Wilk
Konsentrasi
Statistic Df Sig. Statistic df Sig.
Waktu Kematian EEDPT 5 mg/ml
.204
3
.
.993 3 .843
EEDPT 10 mg/ml
.211
3
.
.991 3 .817
EEDPT 20 mg/ml
.337
3
.
.855 3 .253
EEDPT 30 mg/ml
.219
3
.
.987 3 .780
Albendazole 20 mg/ml
.232
3
.
.980 3 .726
a. Lilliefors Significance Correction
Descriptives
95% Confidence
Interval for Mean
N
EEDPT 5
mg/ml
EEDPT 10
mg/ml
EEDPT 20
mg/ml
EEDPT 30
mg/ml
Albendazole
20 mg/ml
Total
Mean
Std.
Std.
Lower
Upper
Deviation
Error
Bound
Bound
Minimum Maximum
3 187.6667
7.02377 4.05518
170.2187
205.1147
181.00
195.00
3 101.3333
6.02771 3.48010
86.3597
116.3070
95.00
107.00
3 50.3333
7.57188 4.37163
31.5237
69.1429
45.00
59.00
3 33.6667
2.51661 1.45297
27.4151
39.9183
31.00
36.00
3 185.6667
4.04145 2.33333
175.6271
195.7062
182.00
190.00
15 111.7333 67.58325 17.44992
74.3070
149.1597
31.00
195.00
Between Groups
Within Groups
Total
Sum of Squares
63613.600
331.333
63944.933
ANOVA
Df
Mean Square
4
15903.400
10
33.133
14
F
479.982
Sig.
.000
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Multiple Comparisons
Dependent Variable: Waktu Kematian
Tukey HSD
Mean
Std.
(J) Konsentrasi Difference (I-J) Error
EEDPT 10
86.33333* 4.69988
mg/ml
EEDPT 20
137.33333* 4.69988
mg/ml
EEDPT 30
154.00000* 4.69988
mg/ml
Albendazole 20
2.00000 4.69988
mg/ml
EEDPT 10
EEDPT 5
-86.33333* 4.69988
mg/ml
mg/ml
EEDPT 20
51.00000* 4.69988
mg/ml
EEDPT 30
67.66667* 4.69988
mg/ml
Albendazole 20
-84.33333* 4.69988
mg/ml
EEDPT 20
EEDPT 5
-137.33333* 4.69988
mg/ml
mg/ml
EEDPT 10
-51.00000* 4.69988
mg/ml
EEDPT 30
16.66667* 4.69988
mg/ml
Albendazole 20
-135.33333* 4.69988
mg/ml
EEDPT 30
EEDPT 5
-154.00000* 4.69988
mg/ml
mg/ml
EEDPT 10
-67.66667* 4.69988
mg/ml
EEDPT 20
-16.66667* 4.69988
mg/ml
Albendazole 20
-152.00000* 4.69988
mg/ml
Albendazole 20 EEDPT 5
-2.00000 4.69988
mg/ml
mg/ml
EEDPT 10
84.33333* 4.69988
mg/ml
EEDPT 20
135.33333* 4.69988
mg/ml
EEDPT 30
152.00000* 4.69988
mg/ml
*. The mean difference is significant at the 0.05 level.
(I) Konsentrasi
EEDPT 5
mg/ml
Sig.
95% Confidence
Interval
Lower
Upper
Bound
Bound
.000
70.8656 101.8010
.000 121.8656 152.8010
.000 138.5323 169.4677
.992
-13.4677
17.4677
.000 -101.8010
-70.8656
.000
35.5323
66.4677
.000
52.1990
83.1344
.000
-99.8010
-68.8656
.000 -152.8010 -121.8656
.000
-66.4677
-35.5323
.034
1.1990
32.1344
.000 -150.8010 -119.8656
.000 -169.4677 -138.5323
.000
-83.1344
-52.1990
.034
-32.1344
-1.1990
.000 -167.4677 -136.5323
.992
-17.4677
13.4677
.000
68.8656
99.8010
.000 119.8656 150.8010
.000 136.5323 167.4677
82
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Death Time
Tukey HSDa
Subset for alpha = 0.05
2
3
Konsentrasi
N
1
EEDPT 30 mg/ml
3
33.6667
EEDPT 20 mg/ml
3
50.3333
EEDPT 10 mg/ml
3
Albendazole 20 mg/ml
3
EEDPT 5 mg/ml
3
Sig.
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
4
101.3333
1.000
185.6667
187.6667
.992
83
Universitas Sumatera Utara
51
Universitas Sumatera Utara
Lampiran 2. Hasil identifikasi hewan percobaan
52
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Lampiran 3. Surat keterangan baku obat
53
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54
Universitas Sumatera Utara
Lampiran 4. Gambar tumbuhan dan daun pugun tanoh
Tumbuhan pugun tanoh
Daun pugun tanoh segar
55
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Lampiran 5. Gambar hewan percobaan
Cacing tanah Pheretima posthuma
56
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Lampiran 6. Gambar simplisia dan serbuk simplisia daun pugun tanoh
Simplisia daun pugun tanoh
Serbuk simplisia daun pugun tanoh
57
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Lampiran 7. Gambar mikroskopik simplisia daun pugun tanoh dengan
perbesaran 10 x 40
a
b
c
a
d
e
f
g
h
i
Keterangan:
a. Berkas pembuluh angkut bentuk spiral
b. Rambut kelenjar
c. Epidermis
d. Tulang daun
e. Rambut multiseluler
f. Kristal Ca Oksalat bentuk prisma
g. Stomata tipe anomositik
h. Stomata tipe diasitik
i. Sel tetangga
58
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Lampiran 8. Ekstrak etilasetat daun pugun tanoh
Ekstrak etilasetat daun pugun tanoh
59
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Lampiran 9. Perhitungan rendemen simplisia dan ekstrak
Berat basah
2100 g
Berat simplisia
600 g
% Rendemen simplisia =
erat sim lisia
x 100%
erat basa
=
x 100% = 28,57%
% Rendemen ekstrak =
erat ekstrak
x 100%
erat sim lisia
=
,
Berat ekstrak
16, 09 g
x 100% = 2,68%
60
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Lampiran 10. Perhitungan kadar air simplisia
% Kadar air =
No.
1
2
3
olume air (ml)
erat sam el ( )
Berat sampel (g)
5,021
5,010
5, 021
% Kadar air =
Volume awal (ml)
1,80
2,00
2,20
olume ak ir- olume a al
erat sam el
1. % Kadar air =
2. % Kadar air =
3. % Kadar air =
,
,
,
x 100% = 3,99%
,
,
,
x 100% = 3,98%
,
% rata-rata kadar air simplisia =
x 100%
x 100% = 3,98%
,
,
Volume akhir (ml)
2,00
2,20
2,40
,
,
,
= 3,98%
61
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Lampiran 11. Perhitungan kadar sari larut air simplisia
% Kadar sari larut dalam air =
No.
1.
2.
3.
berat sari ( )
berat sam el ( )
x
x 100%
Berat sampel (g)
5,025
5,045
5,078
1. % Kadar sari larut dalam air =
2. % Kadar sari larut dalam air =
3. % Kadar sari larut dalam air =
% rata-rata kadar sari larut dalam air =
Berat sari (g)
0,203
0,210
0,199
,
,
,
,
,
,
,
x
x 100% = 20,19%
x
x 100% = 20,81%
x
x 100% = 19,59%
,
,
= 20,20%
62
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Lampiran 12. Perhitungan kadar sari larut etanol simplisia
% Kadar sari larut dalam etanol =
No.
1.
2.
3.
berat sari ( )
berat sam el ( )
x
x 100%
Berat sampel (g)
5,010
5,017
5,007
Berat sari (g)
0,226
0,212
0,198
1. % Kadar sari larut dalam etanol =
2. % Kadar sari larut dalam etanol =
3. % Kadar sari larut dalam etanol =
% rata-rata kadar sari larut dalam etanol =
,
,
,
,
,
,
x
x 100% = 22,55%
x
x 100% = 21,12%
x
x 100% = 19,77%
,
,
= 21,15%
63
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Lampiran 13. Perhitungan kadar abu total simplisia
% Kadar abu total =
No.
1.
2.
3.
berat abu ( )
berat sam el ( )
x 100%
Berat sampel (g)
2,015
2,030
2,024
1. % Kadar abu total =
2. % Kadar abu total =
3. % Kadar abu total =
% rata-rata kadar abu total =
,
Berat abu (g)
0,185
0,180
0,182
x 100% = 9,18%
,
,
x 100% = 8,87%
,
,
x 100% = 8,99%
,
,
,
,
= 9,01%
64
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Lampiran 14. Perhitungan kadar abu tidak larut asam simplisia
% Kadar abu tidak larut asam =
No.
1.
2.
3.
berat abu ( )
berat sam el ( )
x 100%
Berat sampel (g)
2,015
2,030
2,024
1. % Kadar abu tidak larut asam =
2. % Kadar abu tidak larut asam =
3. % Kadar abu tidak larut asam =
% rata-rata kadar abu tidak larut asam =
Berat abu (g)
0,019
0,020
0,017
,
x 100% = 0,94%
,
,
x 100% = 0,99%
,
,
x 100% = 0,84%
,
,
,
,
= 0,92%
65
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Lampiran 15. Perhitungan kadar air ekstrak etilasetat daun pugun tanoh
% Kadar air =
No.
1
2
3
olume air (ml)
erat sam el ( )
x 100%
Berat sampel (g)
5,040
5,040
5,050
1. % Kadar air =
2. % Kadar air =
3. % Kadar air =
,
- ,
,
,
- ,
,
,
- ,
,
Volume awal (ml)
2,35
2,50
2,75
Volume akhir (ml)
2,50
2,75
2,90
x 100% = 2,98%
x 100% = 2,98%
x 100% = 2,97%
% rata-rata kadar air ekstrak =
,
,
,
= 2,98%
66
Universitas Sumatera Utara
Lampiran 16. Perhitungan kadar abu total ekstrak etilasetat daun pugun tanoh
% Kadar abu total =
No.
1.
2.
3.
berat abu ( )
berat sam el ( )
Berat sampel (g)
2,002
2,030
2,024
1. % Kadar abu total =
2. % Kadar abu total =
3. % Kadar abu total =
,
,
,
,
,
,
% rata-rata kadar abu total =
x 100%
Berat abu (g)
0,077
0,081
0,070
x 100% = 3,86%
x 100% = 3,99%
x 100% = 3,46%
,
,
,
= 3,77%
67
Universitas Sumatera Utara
Lampiran 17. Perhitungan kadar abu tidak larut asam ekstrak etilasetat daun
pugun tanoh
% Kadar abu total =
No.
1.
2.
3.
berat abu ( )
berat sam el ( )
x 100%
Berat sampel (g)
2,002
2,030
2,024
1. % Kadar abu tidak larut asam =
2. % Kadar abu tidak larut asam =
3. % Kadar abu tidak larut asam =
Berat abu (g)
0,007
0,008
0,007
,
,
,
,
,
,
% rata-rata kadar abu tidak larut asam =
x 100% = 0,35%
x 100% = 0,39%
x 100% = 0,35%
,
,
,
= 0,36%
68
Universitas Sumatera Utara
Lampiran 18. Hasil uji orientasi pelarut
Konsentrasi Tween
80 (%)
0,5
1
1,5
Pengamatan Terhadap Cacing Pheretima posthuma
Kondisi
Waktu Kematian (menit)
Hidup
Hidup
Mati
284±2,82
69
Universitas Sumatera Utara
Lampiran 19. Uji aktivitas antelmintik
(1)
(2)
(3)
70
Universitas Sumatera Utara
(4)
(5)
(6)
(7)
(8)
(9)
71
Universitas Sumatera Utara
(10)
(11)
(12)
Keterangan :
1. Suspensi ekstrak etilasetat daun pugun tanoh
2. Kontrol negatif dengan larutan NaCl 0,9%
3. Kontrol pelarut dengan Tween 80 1%
4. Pemberian suspensi EEDPT kons. 5 mg/ml
5. Perubahan morfologi cacing
6. Pemberian suspensi EEDPT kons. 10 mg/ml
7. Perubahan morfologi cacing
8. Pemberian suspensi EEDPT kons.15 mg/ml
9. Perubahan morfologi cacing
10. Pemberian suspensi EEDPT kons. 20 mg/ml
11. Perubahan morfologi cacing
12. Kontrol positif dengan suspensi albendazole kons. 20 mg/ml
72
Universitas Sumatera Utara
Lampiran 20. Waktu paralisis cacing
Perlakuan
Pengujian (menit)
I
II
Larutan NaCl 0,9% (kontrol negatif)
‾
‾
Tween 80 1% (kontrol pelarut)
‾
‾
EEDPT 5 mg/ml
67
65
EEDPT 10 mg/ml
67
71
EEDPT 20 mg/ml
37
37
EEDPT 30 mg/ml
30
24
Albendazole 20 mg /ml (kontrol positif)
78
73
Keterangan : EEDPT = Ekstrak Etilasetat Daun Pugun tanoh
III
‾
‾
70
73
42
25
79
73
Universitas Sumatera Utara
Lampiran 21. Waktu kematian cacing
Perlakuan
Pengujian (menit)
I
II
Larutan NaCl 0,9% (kontrol negatif)
‾
‾
Tween 80 1% (kontrol pelarut)
‾
‾
EEDPT 5 mg/ml
181
187
EEDPT 10 mg/ml
107
95
EEDPT 20 mg/ml
47
45
EEDPT 30 mg/ml
36
31
Albendazole 20 mg /ml (kontrol positif)
185
190
Keterangan : EEDPT = Ekstrak Etilasetat Daun Pugun tanoh
III
‾
‾
195
102
59
34
182
74
Universitas Sumatera Utara
Lampiran 22. Uji statistika waktu paralisis cacing
Case Processing Summary
Waktu paralisis
Konsentrasi
EEDPT 5 mg/ml
EEDPT 10 mg/ml
EEDPT 20 mg/ml
EEDPT 30 mg/ml
Albendazole 20 mg/ml
N
3
3
3
3
3
Valid
Percent
100.0%
100.0%
100.0%
100.0%
100.0%
Cases
Missing
N Percent
0 0.0%
0 0.0%
0 0.0%
0 0.0%
0 0.0%
N
3
3
3
3
3
Total
Percent
100.0%
100.0%
100.0%
100.0%
100.0%
Descriptives
Konsentrasi
Waktu paralisis EEDPT 5
mg/ml
EEDPT 10
mg/ml
Mean
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Std.
Statistic Error
67.3333 1.45297
61.0817
73.5849
.
67.0000
6.333
2.51661
65.00
70.00
5.00
.
.586
1.225
.
.
70.3333 1.76383
62.7442
77.9225
.
71.0000
9.333
3.05505
67.00
73.00
6.00
.
-.935
.
1.225
.
75
Universitas Sumatera Utara
EEDPT 20
mg/ml
Mean
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
EEDPT 30
mg/ml
Mean
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Albendazole 20 Mean
mg/ml
95% Confidence Interval Lower
for Mean
Bound
Upper
Bound
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
38.6667 1.66667
31.4956
45.8378
.
37.0000
8.333
2.88675
37.00
42.00
5.00
.
1.732
.
1.225
.
26.3333 1.85592
18.3479
34.3187
.
25.0000
10.333
3.21455
24.00
30.00
6.00
.
1.545
1.225
.
.
76.6667 1.85592
68.6813
84.6521
.
78.0000
10.333
3.21455
73.00
79.00
6.00
.
-1.545
.
1.225
.
76
Universitas Sumatera Utara
Test of Normality
Kolmogorov-Smirnova
Shapiro-Wilk
Konsentrasi
Statistic
df Sig. Statistic df Sig.
Waktu paralisis EEDPT 5 mg/ml
.219 3
.
.987 3 .780
EEDPT 10 mg/ml
.253 3
.
.964 3 .637
EEDPT 20 mg/ml
.385 3
.
.750 3 .000
EEDPT 30 mg/ml
.328 3
.
.871 3 .298
Albendazole 20 mg/ml
.328 3
.
.871 3 .298
a. Lilliefors Significance Correction
Kruskall-Wallis Test
Ranks
Waktu Paralisis
Konsentrasi
EEDPT 5 mg/ml
EEDPT 10 mg/ml
EEDPT 20 mg/ml
EEDPT 30 mg/ml
Total
N
Mean Rank
3
3
3
3
12
8.50
10.50
5.00
2.00
Test Statistics
Waktu_Paralisi
s
Chi-Square
9.877
df
3
Asymp. Sig.
.020
a. Kruskal Wallis Test
b. Grouping Variable:
Konsentrasi
Multiple Comparisons
Dependent Variable: Waktuparalisis
Tukey HSD
(I) Konsentrasi
EEDPT 5
mg/ml
(J) Konsentrasi
EEDPT 10
mg/ml
EEDPT 20
mg/ml
EEDPT 30
mg/ml
Albendazole 20
mg/ml
Mean
Difference (I-J)
Std.
Error
Sig.
95% Confidence
Interval
Lower
Upper
Bound
Bound
-3.00000 2.44040 .736 -11.0316
5.0316
28.66667* 2.44040 .000
20.6351
36.6982
41.00000* 2.44040 .000
32.9684
49.0316
-9.33333* 2.44040 .022 -17.3649
-1.3018
77
Universitas Sumatera Utara
EEDPT 10
mg/ml
EEDPT 5
3.00000 2.44040
mg/ml
EEDPT 20
31.66667* 2.44040
mg/ml
EEDPT 30
44.00000* 2.44040
mg/ml
Albendazole 20
-6.33333 2.44040
mg/ml
EEDPT 20
EEDPT 5
-28.66667* 2.44040
mg/ml
mg/ml
EEDPT 10
-31.66667* 2.44040
mg/ml
EEDPT 30
12.33333* 2.44040
mg/ml
Albendazole 20
-38.00000* 2.44040
mg/ml
EEDPT 30
EEDPT 5
-41.00000* 2.44040
mg/ml
mg/ml
EEDPT 10
-44.00000* 2.44040
mg/ml
EEDPT 20
-12.33333* 2.44040
mg/ml
Albendazole 20
-50.33333* 2.44040
mg/ml
Albendazole 20 EEDPT 5
9.33333* 2.44040
mg/ml
mg/ml
EEDPT 10
6.33333 2.44040
mg/ml
EEDPT 20
38.00000* 2.44040
mg/ml
EEDPT 30
50.33333* 2.44040
mg/ml
*. The mean difference is significant at the 0.05 level.
.736
-5.0316
11.0316
.000
23.6351
39.6982
.000
35.9684
52.0316
.145 -14.3649
1.6982
.000 -36.6982 -20.6351
.000 -39.6982 -23.6351
.004
4.3018
20.3649
.000 -46.0316 -29.9684
.000 -49.0316 -32.9684
.000 -52.0316 -35.9684
.004 -20.3649
-4.3018
.000 -58.3649 -42.3018
.022
1.3018
17.3649
.145
-1.6982
14.3649
.000
29.9684
46.0316
.000
42.3018
58.3649
Paralysis Time
Tukey HSDa
Subset for alpha = 0.05
2
3
Konsentrasi
N
1
EEDPT 30 mg/ml
3
26.3333
EEDPT 20 mg/ml
3
38.6667
EEDPT 5 mg/ml
3
EEDPT 10 mg/ml
3
Albendazole 20 mg/ml
3
Sig.
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
67.3333
70.3333
.736
4
70.3333
76.6667
.145
78
Universitas Sumatera Utara
Lampiran 23. Uji statistika waktu kematian cacing
Case Processing Summary
Waktu Kematian
Konsentrasi
EEDPT 5 mg/ml
EEDPT 10 mg/ml
EEDPT 20 mg/ml
EEDPT 30 mg/ml
Albendazole 20 mg/ml
N
3
3
3
3
3
Valid
Percent
100.0%
100.0%
100.0%
100.0%
100.0%
Cases
Missing
N Percent
0
0.0%
0
0.0%
0
0.0%
0
0.0%
0
0.0%
N
3
3
3
3
3
Total
Percent
100.0%
100.0%
100.0%
100.0%
100.0%
Descriptives
Konsentrasi
Waktu Kematian EEDPT 5
mg/ml
EEDPT 10
mg/ml
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Std.
Statistic Error
187.6667 4.05518
Lower
Bound
Upper
Bound
170.2187
205.1147
.
187.0000
49.333
7.02377
181.00
195.00
14.00
.
.423
1.225
.
.
101.3333 3.48010
Lower
Bound
Upper
Bound
86.3597
116.3070
.
102.0000
36.333
6.02771
95.00
107.00
12.00
.
-.492
.
1.225
.
79
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EEDPT 20
mg/ml
EEDPT 30
mg/ml
Albendazole
20 mg/ml
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
Mean
95% Confidence
Interval for Mean
5% Trimmed Mean
Median
Variance
Std. Deviation
Minimum
Maximum
Range
Interquartile Range
Skewness
Kurtosis
50.3333 4.37163
Lower
Bound
Upper
Bound
31.5237
69.1429
.
47.0000
57.333
7.57188
45.00
59.00
14.00
.
1.597
1.225
.
.
33.6667 1.45297
Lower
Bound
Upper
Bound
27.4151
39.9183
.
34.0000
6.333
2.51661
31.00
36.00
5.00
.
-.586
1.225
.
.
185.6667 2.33333
Lower
Bound
Upper
Bound
175.6271
195.7062
.
185.0000
16.333
4.04145
182.00
190.00
8.00
.
.722
.
1.225
.
80
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Normality Test
Kolmogorov-Smirnova
Shapiro-Wilk
Konsentrasi
Statistic Df Sig. Statistic df Sig.
Waktu Kematian EEDPT 5 mg/ml
.204
3
.
.993 3 .843
EEDPT 10 mg/ml
.211
3
.
.991 3 .817
EEDPT 20 mg/ml
.337
3
.
.855 3 .253
EEDPT 30 mg/ml
.219
3
.
.987 3 .780
Albendazole 20 mg/ml
.232
3
.
.980 3 .726
a. Lilliefors Significance Correction
Descriptives
95% Confidence
Interval for Mean
N
EEDPT 5
mg/ml
EEDPT 10
mg/ml
EEDPT 20
mg/ml
EEDPT 30
mg/ml
Albendazole
20 mg/ml
Total
Mean
Std.
Std.
Lower
Upper
Deviation
Error
Bound
Bound
Minimum Maximum
3 187.6667
7.02377 4.05518
170.2187
205.1147
181.00
195.00
3 101.3333
6.02771 3.48010
86.3597
116.3070
95.00
107.00
3 50.3333
7.57188 4.37163
31.5237
69.1429
45.00
59.00
3 33.6667
2.51661 1.45297
27.4151
39.9183
31.00
36.00
3 185.6667
4.04145 2.33333
175.6271
195.7062
182.00
190.00
15 111.7333 67.58325 17.44992
74.3070
149.1597
31.00
195.00
Between Groups
Within Groups
Total
Sum of Squares
63613.600
331.333
63944.933
ANOVA
Df
Mean Square
4
15903.400
10
33.133
14
F
479.982
Sig.
.000
81
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Multiple Comparisons
Dependent Variable: Waktu Kematian
Tukey HSD
Mean
Std.
(J) Konsentrasi Difference (I-J) Error
EEDPT 10
86.33333* 4.69988
mg/ml
EEDPT 20
137.33333* 4.69988
mg/ml
EEDPT 30
154.00000* 4.69988
mg/ml
Albendazole 20
2.00000 4.69988
mg/ml
EEDPT 10
EEDPT 5
-86.33333* 4.69988
mg/ml
mg/ml
EEDPT 20
51.00000* 4.69988
mg/ml
EEDPT 30
67.66667* 4.69988
mg/ml
Albendazole 20
-84.33333* 4.69988
mg/ml
EEDPT 20
EEDPT 5
-137.33333* 4.69988
mg/ml
mg/ml
EEDPT 10
-51.00000* 4.69988
mg/ml
EEDPT 30
16.66667* 4.69988
mg/ml
Albendazole 20
-135.33333* 4.69988
mg/ml
EEDPT 30
EEDPT 5
-154.00000* 4.69988
mg/ml
mg/ml
EEDPT 10
-67.66667* 4.69988
mg/ml
EEDPT 20
-16.66667* 4.69988
mg/ml
Albendazole 20
-152.00000* 4.69988
mg/ml
Albendazole 20 EEDPT 5
-2.00000 4.69988
mg/ml
mg/ml
EEDPT 10
84.33333* 4.69988
mg/ml
EEDPT 20
135.33333* 4.69988
mg/ml
EEDPT 30
152.00000* 4.69988
mg/ml
*. The mean difference is significant at the 0.05 level.
(I) Konsentrasi
EEDPT 5
mg/ml
Sig.
95% Confidence
Interval
Lower
Upper
Bound
Bound
.000
70.8656 101.8010
.000 121.8656 152.8010
.000 138.5323 169.4677
.992
-13.4677
17.4677
.000 -101.8010
-70.8656
.000
35.5323
66.4677
.000
52.1990
83.1344
.000
-99.8010
-68.8656
.000 -152.8010 -121.8656
.000
-66.4677
-35.5323
.034
1.1990
32.1344
.000 -150.8010 -119.8656
.000 -169.4677 -138.5323
.000
-83.1344
-52.1990
.034
-32.1344
-1.1990
.000 -167.4677 -136.5323
.992
-17.4677
13.4677
.000
68.8656
99.8010
.000 119.8656 150.8010
.000 136.5323 167.4677
82
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Death Time
Tukey HSDa
Subset for alpha = 0.05
2
3
Konsentrasi
N
1
EEDPT 30 mg/ml
3
33.6667
EEDPT 20 mg/ml
3
50.3333
EEDPT 10 mg/ml
3
Albendazole 20 mg/ml
3
EEDPT 5 mg/ml
3
Sig.
1.000
1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
4
101.3333
1.000
185.6667
187.6667
.992
83
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