B1J007144 8 1.
LAMPIRAN
Lampiran 1. Spesifikasi peralatan dan bahan
a. Spesifikasi alat
No.
1
Nama Alat
Derigen
Merek/Tipe
20-25 L
Kegunaan
Tempat penyimpanan
Tempat
Lab. Mikrobiologi
sementara limbah cair
tahu
2
Baskom
8 liter
Tempat perlakuan
Stasiun Percobaan
limbah cair tahu
3
Termometer
Mengukur suhu limbah
celcius
Lab. Lingkungan
cair tahu
4
pH indicator
Mengukur pH limbah
Lab. Lingkungan
5
Botol Winkler
Tempat menampung
Lab. Lingkungan
sampel untuk
pemeriksaan BOD5
6
Erlenmeyer
250 ml
Tempat menampung
Lab. Mikrobiologi
media
7
Cawan petri
Tempat membiakkan
Lab. Mikrobiologi
bakteri
8
Tabung reaksi
Tempat menampung
Lab. Mikrobiologi
akuades untuk
pengenceran sampel
limbah cair tahu
9
Mikropipet dan
1 ml
tip
11
Beker glass
Memindahkan sampel
Lab. Mikrobiologi
dengan volume kecil
1000 ml
Tempat pembuatan
Lab. Mikrobiologi
media
12
Microwave
13
Gelas ukur
250 ml
Membuat media
Lab. Mikrobiologi
Mengukur volume
Lab. Mikrobiologi
akuades
14
Autoklaf
15
Botol sampel
Sterilisasi alat dan media
100 ml
Tempat penampung
sampel limbah cair tahu
21
Lab. Mikrobiologi
Lab. Lingkungan
b. Spesifikasi bahan
1.
No
Nama Bahan
Limbah cair tahu
Spesifikasi
-
Kegunaan
Sampel
2.
EM4 (Effective
cair
Stater
-
Fermentasi EM4
microorganism)
3.
Molase
4.
KOH-KI
Mengikat oksigen
5.
MnSO4
Mengikat oksigen
6.
H2SO4
Memecah endapan
7.
Na2S2O3
8.
Larutan Buffer Fosfat
Pengatur pH
10.
Akuades
Pengenceran
11.
Media NA
Media tumbuh
0,025 N
Titrasi oksigen terlarut
mikroba
12.
Larutan phenat
Pengukuran amoniak
13.
Bunsen
Aseptis kerja
14.
Alkohol
Aseptis kerja
15.
Bubuk inhibitor
Pengukuran amoniak
nitrifikasi
22
Lampiran 2. Data Nilai akhir NH3 Limbah Cair Tahu Selama 6 Hari Perlakuan
A. Data NH3 (ppm)
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
21.8
20.9
22.4 |
65.1 |
21.70 |
0.75
A1 B2
|
18.9
18.8
19.2 |
56.9 |
18.97 |
0.21
A1 B3
|
16.6
16.2
14.8 |
47.6 |
15.87 |
0.95
A1 B4
|
14.5
14.2
12.9 |
41.6 |
13.87 |
0.85
A2 B1
|
17.7
15.9
17.0 |
50.6 |
16.87 |
0.91
A2 B2
|
14.5
12.7
13.5 |
40.7 |
13.57 |
0.90
A2 B3
|
12.5
10.4
9.8 |
32.7 |
10.90 |
1.42
A2 B4
|
9.8
7.8
6.8 |
24.4 |
8.13 |
1.53
A3 B1
|
12.8
11.2
11.5 |
35.5 |
11.83 |
0.85
A3 B2
|
8.7
8.2
9.2 |
26.1 |
8.70 |
0.50
A3 B3
|
6.2
5.8
6.7 |
18.7 |
6.23 |
0.45
A3 B4
|
4.6
4.1
3.8 |
12.5 |
4.17 |
0.40
-------------------------------------------------------------------------Total
|
|
452.4 |
12.57
-------------------------------------------------------------------------SD sampel =
5.17
B. Perhitungan nilai-nilai dispersi dari data NH3 Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
65.1
56.9
47.6
41.6 |
211.2 |
17.60 |
3.18
A2 |
50.6
40.7
32.7
24.4 |
148.4 |
12.37 |
3.53
A3 |
35.5
26.1
18.7
12.5 |
92.8 |
7.73 |
3.03
-----------------------------------------------------------------------------Total |
151.2
123.7
99.0
78.5 |
452.4
Rataan|
16.80
13.74
11.00
8.72|
SD |
4.33
4.48
4.26
4.32|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
71.8
70.1
69.3 |
211.2
A2 |
54.5
46.8
47.1 |
148.4
A3 |
32.3
29.3
31.2 |
92.8
---------------------------------------------------Total |
158.6
146.2
147.6 |
452.4
----------------------------------------------------
23
C. Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
Total
A - B
A - r
A
B
Error
A X B
Error
=
=
=
=
=
=
a
(
(
(
(
(
=
=
b =
452.42 / 36 =
5685.1600
21.82 +...+
3.82 )
- FK =
934.22000
2
65.1 +...+
12.52 ) / 3 - FK =
915.12000
71.82 +...+
31.22 ) / 4 - FK =
596.30500
211.22 +...+
92.82 ) / 12 - FK =
584.82667
151.22 +...+
78.52 ) / 9 - FK =
328.88222
596.30500 - 584.82667 =
11.47833
915.12000 - 584.82667 - 328.88222 =
1.41111
934.22000 - 596.30500 - 915.12000 + 584.82667 =
7.62167
D. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir NH 3
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
584.8267
292.4133 152.8515 **
5.14
10.92
Error a
|
6
11.4783
1.9131
SD a =
1.383
KK a = 11.006 %
B
|
3
328.8822
109.6274 258.9058 **
3.16
5.09
A X B
|
6
1.4111
0.2352
0.5554
2.66
4.01
Error b
| 18
7.6217
0.4234
SD b =
0.651
-------------------------------------------------------------------------Total
| 35
934.2200
KK b =
5.178 %
--------------------------------------------------------------------------
E. Uji DMRT pengaruh perlakuan terhadap nilai akhir NH 3
Tabel : DMRT A
Std. Error :
0.3993
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
1.38
1.43
DMRT 1%|
2.09
2.17
---------------------------------------------Perl. |
A1
A2
A3
Rataan |
17.60
12.37
7.73
---------------------------------------------A1
|
0.00
A2
|
5.23**
0.00
A3
|
9.87**
4.63**
0.00
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A1
2 A2
3 A3
sesuai perlakuan
A1
17.60 a
A2
12.37 b
A3
7.73 c
17.60 a
12.37 b
7.73 c
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
24
Tabel : DMRT B
Std. Error :
0.2169
---------------------------------------------------------Range |
2
3
4
---------------------------------------------------------P 0.05 |
2.97
3.12
3.21
P 0.01 |
4.07
4.25
4.36
DMRT 5%|
0.64
0.68
0.70
DMRT 1%|
0.88
0.92
0.95
---------------------------------------------------------Perl. |
B1
B2
B3
B4
Rataan |
16.80
13.74
11.00
8.72
---------------------------------------------------------B1
|
0.00
B2
|
3.06**
0.00
B3
|
5.80**
2.74**
0.00
B4
|
8.08**
5.02**
2.28**
0.00
---------------------------------------------------------urut rataan
1 B1
2 B2
3 B3
4 B4
16.80
13.74
11.00
8.72
sesuai perlakuan
B1
16.80 a
B2
13.74 b
B3
11.00 c
B4
8.72 d
a
b
c
d
25
Lampiran 3. Data Nilai akhir BOD5 Limbah Cair Tahu Selama 6 Hari Perlakuan
A. Tabulasi Data BOD5 (ppm)
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
| 2000.00 2190.00 2300.00 | 6490.00 | 2163.333 |
151.767
A1 B2
| 1820.00 2060.00 2220.00 | 6100.00 | 2033.333 |
201.329
A1 B3
| 2020.00 2160.00 2270.00 | 6450.00 | 2150.000 |
125.300
A1 B4
| 2040.00 2060.00 2250.00 | 6350.00 | 2116.667 |
115.902
A2 B1
| 2364.00 2404.00 2444.00 | 7212.00 | 2404.000 |
40.000
A2 B2
| 2324.00 1364.00 2284.00 | 5972.00 | 1990.667 |
543.078
A2 B3
| 2284.00 1384.00 2164.00 | 5832.00 | 1944.000 |
488.672
A2 B4
| 2244.00 1364.00 1884.00 | 5492.00 | 1830.667 |
442.418
A3 B1
| 1003.20 1001.80 1002.50 | 3007.50 | 1002.500 |
0.700
A3 B2
|
703.16
699.89
701.33 | 2104.38 |
701.460 |
1.639
A3 B3
|
385.15
384.62
386.04 | 1155.81 |
385.270 |
0.718
A3 B4
|
139.23
140.05
139.88 |
419.16 |
139.720 |
0.433
-------------------------------------------------------------------------Total
|
| 56584.85 | 1571.801
-------------------------------------------------------------------------SD sampel =
794.111
B. Perhitungan nilai-nilai disperse dari data BOD5 Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 | 6490.00 6100.00 6450.00 6350.00 | 25390.00 | 2115.833 |
140.160
A2 | 7212.00 5972.00 5832.00 5492.00 | 24508.00 | 2042.333 |
429.161
A3 | 3007.50 2104.38 1155.81
419.16 | 6686.85 |
557.238 |
339.634
-----------------------------------------------------------------------------Total | 16709.50 14176.38 13437.81 12261.16 | 56584.85
Rataan| 1856.611 1575.153 1493.090 1362.351|
SD |
653.732 716.650 872.879 953.136|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 | 7880.00 8470.00 9040.00 | 25390.00
A2 | 9216.00 6516.00 8776.00 | 24508.00
A3 | 2230.74 2226.36 2229.75 | 6686.85
---------------------------------------------------Total | 19326.74 17212.36 20045.75 | 56584.85
----------------------------------------------------
26
C. Perhitungan Jumlah Kuadrat BOD5
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
Total
A - B
A - r
A
B
Error
A X B
Error
56584.852 / 36 =
88940146
( 2000.002 +...+
139.882 )
( 6490.002 +...+
419.162 )
2
( 7880.00 +...+
2229.752 )
( 25390.002 +...+
6686.852 )
( 16709.502 +...+ 12261.162 )
=
19778017 18560531 =
=
20423914 18560531 b =
22071455 19778017 =
=
=
=
=
=
a
- FK =
/ 3 - FK =
/ 4 - FK =
/ 12 - FK =
/ 9 - FK =
1217486
1180734 =
20423914 +
22071455
20423914
19778017
18560531
1180734
682648
18560531 =
430055
D. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir BOD5
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2 18560531.4235 9280265.7117
45.7349 **
5.14
10.92
Error a
|
6 1217485.9714
202914.3286
SD a = 450.460
KK a = 28.659 %
B
|
3 1180734.4351
393578.1450
16.4733 **
3.16
5.09
A X B
|
6
682648.0868
113774.6811
4.7621 **
2.66
4.01
Error b
| 18
430055.1181
23891.9510
SD b = 154.570
-------------------------------------------------------------------------Total
| 35 22071455.0348
KK b =
9.834 %
--------------------------------------------------------------------------
E. Uji DMRT pengaruh perlakuan terhadap nilai akhir BOD5
Tabel : DMRT A
Std. Error :
130.0366
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
450.06
466.44
DMRT 1%|
681.78
707.27
---------------------------------------------Perl. |
A1
A2
A3
Rataan | 2115.833
2042.333
557.238
---------------------------------------------A1
|
0.000
A2
|
73.500
0.000
A3
| 1558.596** 1485.096**
0.000
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A1
2115.833 a
2 A2
2042.333 a
3 A3
557.238 b
sesuai perlakuan
A1
2115.833 a
A2
2042.333 a
A3
557.238 b
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
27
Tabel : DMRT B
Std. Error :
51.5234
---------------------------------------------------------Range |
2
3
4
---------------------------------------------------------P 0.05 |
2.97
3.12
3.21
P 0.01 |
4.07
4.25
4.36
DMRT 5%|
153.08
160.65
165.39
DMRT 1%|
209.75
218.77
224.75
---------------------------------------------------------Perl. |
B1
B2
B3
B4
Rataan | 1856.611
1575.153
1493.090
1362.351
---------------------------------------------------------B1
|
0.000
B2
|
281.458**
0.000
B3
|
363.521**
82.063
0.000
B4
|
494.260**
212.802*
130.739
0.000
---------------------------------------------------------Pemberian tanda pembeda
urut rataan
1 B1
2 B2
3 B3
4 B4
1856.611
1575.153
1493.090
1362.351
sesuai perlakuan
B1
1856.611 a
B2
1575.153 b
B3
1493.090 bc
B4
1362.351 c
a
b
bc
c
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
Tabel : DMRT A x B
Std. Error :
151.2697
--------------------------------------------------------------------------------------------------------------------------------------------------------Range |
2
3
4
5
6
7
8
9
10
11
12
--------------------------------------------------------------------------------------------------------------------------------------------------------P 0.05 |
2.97
3.12
3.21
3.27
3.32
3.36
3.38
3.41
3.42
3.44
3.45
P 0.01 |
4.07
4.25
4.36
4.45
4.51
4.56
4.60
4.64
4.66
4.69
4.71
DMRT 5%|
449.42
471.66
485.58
495.26
502.37
507.66
511.75
515.07
517.49
519.61
521.12
DMRT 1%|
615.82
642.29
659.84
672.39
682.07
689.79
695.99
701.13
705.52
709.30
712.63
--------------------------------------------------------------------------------------------------------------------------------------------------------Perl. |
A2B1
A1B1
A1B3
A1B4
A1B2
A2B2
A2B3
A2B4
A3B1
A3B2
A3B3
A3B4
Rataan | 2404.000
2163.333
2150.000
2116.667
2033.333
1990.667
1944.000
1830.667
1002.500
701.460
385.270
139.720
--------------------------------------------------------------------------------------------------------------------------------------------------------A2B1 |
0.000
A1B1 |
240.667
0.000
A1B3 |
254.000
13.333
0.000
A1B4 |
287.333
46.667
33.333
0.000
A1B2 |
370.667
130.000
116.667
83.333
0.000
A2B2 |
413.333
172.667
159.333
126.000
42.667
0.000
A2B3 |
460.000
219.333
206.000
172.667
89.333
46.667
0.000
A2B4 |
573.333*
332.667
319.333
286.000
202.667
160.000
113.333
0.000
A3B1 | 1401.500** 1160.833** 1147.500** 1114.167** 1030.833**
988.167**
941.500**
828.167**
0.000
A3B2 | 1702.540** 1461.873** 1448.540** 1415.207** 1331.873** 1289.207** 1242.540** 1129.207**
301.040
0.000
A3B3 | 2018.730** 1778.063** 1764.730** 1731.397** 1648.063** 1605.397** 1558.730** 1445.397**
617.230*
316.190
0.000
A3B4 | 2264.280** 2023.613** 2010.280** 1976.947** 1893.613** 1850.947** 1804.280** 1690.947**
862.780**
561.740*
245.550
0.000
---------------------------------------------------------------------------------------------------------------------------------------------------------
1
2
3
4
5
6
7
8
9
10
11
12
urut rataan
A2B1
2404.000
A1B1
2163.333
A1B3
2150.000
A1B4
2116.667
A1B2
2033.333
A2B2
1990.667
A2B3
1944.000
A2B4
1830.667
A3B1
1002.500
A3B2
701.460
A3B3
385.270
A3B4
139.720
sesuai perlakuan
A1B1
2163.333 ab
A1B2
2033.333 ab
A1B3
2150.000 ab
A1B4
2116.667 ab
A2B1
2404.000 a
A2B2
1990.667 ab
A2B3
1944.000 ab
A2B4
1830.667 b
A3B1
1002.500 c
A3B2
701.460 cd
A3B3
385.270 de
A3B4
139.720 e
a
ab
ab
ab
ab
ab
ab
b
c
cd
de
e
28
Lampiran 4. Data Nilai akhir pH Limbah Cair Tahu Selama 6 Hari Perlakuan
A.Tabulasi Data pH
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
4
4
4 |
12 |
4.0 |
0.0
A1 B2
|
4
4
4 |
12 |
4.0 |
0.0
A1 B3
|
4
4
4 |
12 |
4.0 |
0.0
A1 B4
|
4
4
4 |
12 |
4.0 |
0.0
A2 B1
|
5
4
4 |
13 |
4.3 |
0.6
A2 B2
|
5
4
5 |
14 |
4.7 |
0.6
A2 B3
|
4
5
5 |
14 |
4.7 |
0.6
A2 B4
|
4
5
4 |
13 |
4.3 |
0.6
A3 B1
|
8
6
7 |
21 |
7.0 |
1.0
A3 B2
|
5
7
5 |
17 |
5.7 |
1.2
A3 B3
|
8
5
8 |
21 |
7.0 |
1.7
A3 B4
|
5
5
5 |
15 |
5.0 |
0.0
-------------------------------------------------------------------------Total
|
|
176 |
4.9
-------------------------------------------------------------------------SD sampel =
1.2
B. Perhitungan nilai-nilai dispersi dari data pH Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
12
12
12
12 |
48 |
4.0 |
0.0
A2 |
13
14
14
13 |
54 |
4.5 |
0.5
A3 |
21
17
21
15 |
74 |
6.2 |
1.3
-----------------------------------------------------------------------------Total |
46
43
47
40 |
176
Rataan|
5.1
4.8
5.2
4.4|
SD |
1.5
1.0
1.6
0.5|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
16
16
16 |
48
A2 |
18
18
18 |
54
A3 |
26
23
25 |
74
---------------------------------------------------Total |
60
57
59 |
176
----------------------------------------------------
29
C. Perhitungan Jumlah Kuadrat pH
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
Total
A - B
A - r
A
B
Error
A X B
Error
=
=
=
=
=
=
a
(
(
(
(
(
=
=
b =
176^2 / 36 =
860.44444
42 +...+
52 )
- FK =
53.555556
122 +...+
152 ) / 3 - FK =
40.222222
162 +...+
252 ) / 4 - FK =
32.055556
482 +...+
742 ) / 12 - FK =
30.888889
462 +...+
402 ) / 9 - FK =
3.333333
32.055556 - 30.888889 =
1.166667
40.222222 - 30.888889 3.333333 =
6.000000
53.555556 - 32.055556 - 40.222222 + 30.888889 =
12.166667
D. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir pH
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
30.8889
15.4444
79.4286 **
5.14
10.92
Error a
|
6
1.1667
0.1944
SD a =
0.441
KK a =
9.020 %
B
|
3
3.3333
1.1111
1.6438
3.16
5.09
A X B
|
6
6.0000
1.0000
1.4795
2.66
4.01
Error b
| 18
12.1667
0.6759
SD b =
0.822
-------------------------------------------------------------------------Total
| 35
53.5556
KK b = 16.817 %
--------------------------------------------------------------------------
E. Uji DMRT pengaruh perlakuan terhadap nilai akhir pH
Tabel : DMRT A
Std. Error :
0.1273
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
0.44
0.46
DMRT 1%|
0.67
0.69
---------------------------------------------Perl. |
A3
A2
A1
Rataan |
6.2
4.5
4.0
---------------------------------------------A3
|
0.0
A2
|
1.7**
0.0
A1
|
2.2**
0.5*
0.0
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A3
2 A2
3 A1
sesuai perlakuan
A1
4.0 c
A2
4.5 b
A3
6.2 a
6.2 a
4.5 b
4.0 c
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
30
Lampiran 5. Data Nilai akhir Suhu Limbah Cair Tahu Selama 6 Hari Perlakuan
A. Data Suhu OC
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
27
27
27 |
81 |
27.0 |
0.0
A1 B2
|
27
27
27 |
81 |
27.0 |
0.0
A1 B3
|
27
27
27 |
81 |
27.0 |
0.0
A1 B4
|
27
27
27 |
81 |
27.0 |
0.0
A2 B1
|
27
26
26 |
79 |
26.3 |
0.6
A2 B2
|
26
27
26 |
79 |
26.3 |
0.6
A2 B3
|
27
27
26 |
80 |
26.7 |
0.6
A2 B4
|
26
28
26 |
80 |
26.7 |
1.2
A3 B1
|
28
28
28 |
84 |
28.0 |
0.0
A3 B2
|
28
28
28 |
84 |
28.0 |
0.0
A3 B3
|
28
28
28 |
84 |
28.0 |
0.0
A3 B4
|
28
28
28 |
84 |
28.0 |
0.0
-------------------------------------------------------------------------Total
|
|
978 |
27.2
-------------------------------------------------------------------------SD sampel =
0.7
B. Perhitungan nilai-nilai dispersi dari data suhu Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
81
81
81
81 |
324 |
27.0 |
0.0
A2 |
79
79
80
80 |
318 |
26.5 |
0.7
A3 |
84
84
84
84 |
336 |
28.0 |
0.0
-----------------------------------------------------------------------------Total |
244
244
245
245 |
978
Rataan|
27.1
27.1
27.2
27.2|
SD |
0.8
0.8
0.7
0.8|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
108
108
108 |
324
A2 |
106
108
104 |
318
A3 |
112
112
112 |
336
---------------------------------------------------Total |
326
328
324 |
978
----------------------------------------------------
31
C. Perhitungan Jumlah Kuadrat Suhu
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
9782/ 36 =
26569.000
272 +...+
282 )
- FK =
19.000000
812 +...+
842 ) / 3 - FK =
14.333333
1082 +...+
1122) / 4 - FK =
16.000000
3242 +...+
3362 ) / 12 - FK =
14.000000
2442 +...+
2452 ) / 9 - FK =
0.111111
16.000000 - 14.000000 =
2.000000
14.333333 - 14.000000 0.111111 =
0.222222
19.000000 - 16.000000 - 14.333333 + 14.000000 =
2.666667
D. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir suhu
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
14.0000
7.0000
21.0000 **
5.14
10.92
Error a
|
6
2.0000
0.3333
SD a =
0.577
KK a =
2.125 %
B
|
3
0.1111
0.0370
0.2500
3.16
5.09
A X B
|
6
0.2222
0.0370
0.2500
2.66
4.01
Error b
| 18
2.6667
0.1481
SD b =
0.385
-------------------------------------------------------------------------Total
| 35
19.0000
KK b =
1.417 %
--------------------------------------------------------------------------
E. Uji DMRT pengaruh perlakuan terhadap nilai akhir suhu
Tabel : DMRT A
Std. Error :
0.1667
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
0.58
0.60
DMRT 1%|
0.87
0.91
---------------------------------------------Perl. |
A3
A1
A2
Rataan |
28.0
27.0
26.5
---------------------------------------------A3
|
0.0
A1
|
1.0**
0.0
A2
|
1.5**
0.5
0.0
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A3
2 A1
3 A2
sesuai perlakuan
A1
27.0 b
A2
26.5 b
A3
28.0 a
28.0 a
27.0 b
26.5 b
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
32
Lampiran 6. Data Nilai akhir Jumlah Mikroba Limbah Cair Tahu setelah Perlakuan Selama 6
Hari
Data : Jumlah mikroba
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
8.778
7.301
9.602 |
25.681 |
8.5603 |
1.1658
A1 B2
|
9.602
8.000
9.602 |
27.204 |
9.0680 |
0.9249
A1 B3
|
9.505
8.699
9.602 |
27.806 |
9.2687 |
0.4957
A1 B4
|
8.204
8.301
9.602 |
26.107 |
8.7023 |
0.7806
A2 B1
|
8.699
8.447
8.477 |
25.623 |
8.5410 |
0.1377
A2 B2
|
7.477
7.477
7.477 |
22.431 |
7.4770 |
0.0000
A2 B3
|
8.146
8.000
8.146 |
24.292 |
8.0973 |
0.0843
A2 B4
|
7.845
8.699
8.079 |
24.623 |
8.2077 |
0.4413
A3 B1
|
8.398
8.176
8.279 |
24.853 |
8.2843 |
0.1111
A3 B2
|
8.602
8.204
7.477 |
24.283 |
8.0943 |
0.5705
A3 B3
|
9.380
8.000
8.342 |
25.722 |
8.5740 |
0.7187
A3 B4
|
8.954
7.699
7.903 |
24.556 |
8.1853 |
0.6735
-------------------------------------------------------------------------Total
|
| 303.181 |
8.4217
-------------------------------------------------------------------------SD sampel =
0.6904
B. Perhitungan nilai-nilai dispersi dari data jumlah mikroba Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
25.681
27.204
27.806
26.107 | 106.798 |
8.8998 |
0.8031
A2 |
25.623
22.431
24.292
24.623 |
96.969 |
8.0808 |
0.4492
A3 |
24.853
24.283
25.722
24.556 |
99.414 |
8.2845 |
0.5227
-----------------------------------------------------------------------------Total |
76.157
73.918
77.820
75.286 | 303.181
Rataan|
8.4619
8.2131
8.6467
8.3651|
SD |
0.6045
0.8819
0.6727
0.6152|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
36.089
32.301
38.408 | 106.798
A2 |
32.167
32.623
32.179 |
96.969
A3 |
35.334
32.079
32.001 |
99.414
---------------------------------------------------Total | 103.590
97.003 102.588 | 303.181
----------------------------------------------------
33
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
303.1812 / 36 =
2553.2977
8.7782 +...+
7.9032 )
- FK =
16.684290
2
25.681 +...+
24.5562) / 3 - FK =
7.487544
36.0892 +...+
32.0012 ) / 4 - FK =
10.958967
2
106.798 +...+
99.4142 ) / 12 - FK =
4.364187
76.1572 +...+
75.286^2 ) / 9 - FK =
0.890431
10.958967 4.364187 =
6.594781
7.487544 4.364187 0.890431 =
2.232927
16.684290 - 10.958967 7.487544 +
4.364187 =
2.601965
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir jumlah
mikroba limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
4.3642
2.1821
1.9853
5.14
10.92
Error a
|
6
6.5948
1.0991
SD a =
1.048
KK a = 12.449 %
B
|
3
0.8904
0.2968
2.0533
3.16
5.09
A X B
|
6
2.2329
0.3722
2.5745
2.66
4.01
Error b
| 18
2.6020
0.1446
SD b =
0.380
-------------------------------------------------------------------------Total
| 35
16.6843
KK b =
4.515 %
--------------------------------------------------------------------------
34
Lampiran 7. Data Nilai akhir Jumlah Bau Amoniak Cair Tahu setelah Perlakuan Selama 6 Hari
A. Data Bau Amoniak
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
2.0
2.4
1.8 |
6.2 |
2.07 |
0.31
A1 B2
|
2.3
2.8
2.0 |
7.1 |
2.37 |
0.40
A1 B3
|
2.0
2.3
3.4 |
7.7 |
2.57 |
0.74
A1 B4
|
3.0
2.0
2.3 |
7.3 |
2.43 |
0.51
A2 B1
|
4.0
3.0
2.2 |
9.2 |
3.07 |
0.90
A2 B2
|
2.5
3.3
1.8 |
7.6 |
2.53 |
0.75
A2 B3
|
2.8
3.0
3.0 |
8.8 |
2.93 |
0.12
A2 B4
|
3.0
3.5
4.0 |
10.5 |
3.50 |
0.50
A3 B1
|
3.0
3.2
1.6 |
7.8 |
2.60 |
0.87
A3 B2
|
3.2
2.8
2.6 |
8.6 |
2.87 |
0.31
A3 B3
|
2.8
3.0
2.0 |
7.8 |
2.60 |
0.53
A3 B4
|
3.1
1.6
3.1 |
7.8 |
2.60 |
0.87
-------------------------------------------------------------------------Total
|
|
96.4 |
2.68
-------------------------------------------------------------------------SD sampel =
0.63
B. Perhitungan nilai-nilai dispersi dari data bau amoniak Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
6.2
7.1
7.7
7.3 |
28.3 |
2.36 |
0.48
A2 |
9.2
7.6
8.8
10.5 |
36.1 |
3.01 |
0.65
A3 |
7.8
8.6
7.8
7.8 |
32.0 |
2.67 |
0.60
-----------------------------------------------------------------------------Total |
23.2
23.3
24.3
25.6 |
96.4
Rataan|
2.58
2.59
2.70
2.84|
SD |
0.78
0.50
0.49
0.75|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
9.3
9.5
9.5 |
28.3
A2 |
12.3
12.8
11.0 |
36.1
A3 |
12.1
10.6
9.3 |
32.0
---------------------------------------------------Total |
33.7
32.9
29.8 |
96.4
----------------------------------------------------
35
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
96.42 / 36 =
258.13778
2.02 +...+
3.12 )
- FK =
2
6.2 +...+
7.82) / 3 - FK =
9.32 +...+
9.32 ) / 4 - FK =
2
28.3 +...+
32.02 ) / 12 - FK =
23.22 +...+
25.62 ) / 9 - FK =
3.957222 2.537222 =
1.420000
4.528889 2.537222 0.415556 =
13.702222 3.957222 4.528889 +
13.702222
4.528889
3.957222
2.537222
0.415556
1.576111
2.537222 =
7.753333
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir bau
amoniak limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
2.5372
1.2686
5.3603 *
5.14
10.92
Error a
|
6
1.4200
0.2367
SD a =
0.486
KK a = 18.167 %
B
|
3
0.4156
0.1385
0.3216
3.16
5.09
A X B
|
6
1.5761
0.2627
0.6098
2.66
4.01
Error b
| 18
7.7533
0.4307
SD b =
0.656
-------------------------------------------------------------------------Total
| 35
13.7022
KK b = 24.509 %
--------------------------------------------------------------------------
D. Uji DMRT pengaruh perlakuan terhadap nilai akhir bau amoniak
Tabel : DMRT A
Std. Error :
0.1404
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
0.49
0.50
DMRT 1%|
0.74
0.76
---------------------------------------------Perl. |
A2
A3
A1
Rataan |
3.01
2.67
2.36
---------------------------------------------A2
|
0.00
A3
|
0.34
0.00
A1
|
0.65*
0.31
0.00
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A2
2 A3
3 A1
sesuai perlakuan
A1
2.36 b
A2
3.01 a
A3
2.67 ab
3.01 a
2.67 ab
2.36 b
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
36
Lampiran 8. Data Nilai akhir Bau H2S Limbah Cair Tahu setelah Perlakuan Selama 6 Hari
A. Data Bau H2S
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
3.2
2.9
3.4 |
9.5 |
3.17 |
0.25
A1 B2
|
3.1
3.4
3.1 |
9.6 |
3.20 |
0.17
A1 B3
|
2.4
3.8
2.8 |
9.0 |
3.00 |
0.72
A1 B4
|
3.0
2.6
3.0 |
8.6 |
2.87 |
0.23
A2 B1
|
3.3
3.3
3.3 |
9.9 |
3.30 |
0.00
A2 B2
|
3.3
3.3
3.1 |
9.7 |
3.23 |
0.12
A2 B3
|
3.6
3.3
3.8 |
10.7 |
3.57 |
0.25
A2 B4
|
2.7
3.4
3.7 |
9.8 |
3.27 |
0.51
A3 B1
|
3.4
3.5
2.9 |
9.8 |
3.27 |
0.32
A3 B2
|
3.7
3.5
2.9 |
10.1 |
3.37 |
0.42
A3 B3
|
3.5
3.4
3.5 |
10.4 |
3.47 |
0.06
A3 B4
|
3.6
2.9
3.5 |
10.0 |
3.33 |
0.38
-------------------------------------------------------------------------Total
|
|
117.1 |
3.25
-------------------------------------------------------------------------SD sampel =
0.34
B. Perhitungan nilai-nilai dispersi dari data bau H2S Limbah Cair Tahu
Tabel : A X B
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
9.5
9.6
9.0
8.6 |
36.7 |
3.06 |
0.38
A2 |
9.9
9.7
10.7
9.8 |
40.1 |
3.34 |
0.28
A3 |
9.8
10.1
10.4
10.0 |
40.3 |
3.36 |
0.29
-----------------------------------------------------------------------------Total |
29.2
29.4
30.1
28.4 |
117.1
Rataan|
3.24
3.27
3.34
3.16|
SD |
0.21
0.24
0.46
0.40|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
11.7
12.7
12.3 |
36.7
A2 |
12.9
13.3
13.9 |
40.1
A3 |
14.2
13.3
12.8 |
40.3
---------------------------------------------------Total |
38.8
39.3
39.0 |
117.1
----------------------------------------------------
37
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
117.12 / 36 =
380.90028
3.22 +...+
3.52 )
- FK =
4.0297222
2
9.5 +...+
10.02 ) / 3 - FK =
1.1697222
11.72 +...+
12.82 ) / 4 - FK =
1.1872222
2
36.7 +...+
40.32 ) / 12 - FK =
0.6822222
29.22 +...+
28.42 ) / 9 - FK =
0.1630556
1.1872222 - 0.6822222 =
0.5050000
1.1697222 - 0.6822222 - 0.1630556 =
0.3244444
4.0297222 - 1.1872222 - 1.1697222 + 0.6822222 =
2.3550000
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir bau H2S
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
0.6822
0.3411
4.0528
5.14
10.92
Error a
|
6
0.5050
0.0842
SD a =
0.290
KK a =
8.919 %
B
|
3
0.1631
0.0544
0.4154
3.16
5.09
A X B
|
6
0.3244
0.0541
0.4133
2.66
4.01
Error b
| 18
2.3550
0.1308
SD b =
0.362
-------------------------------------------------------------------------Total
| 35
4.0297
KK b = 11.120 %
--------------------------------------------------------------------------
38
Lampiran 9. Data Nilai akhir Bau Asam Limbah Cair Tahu setelah Perlakuan Selama 6 Hari
A. Data Bau Asam
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
2.8
2.7
2.9 |
8.4 |
2.80 |
0.10
A1 B2
|
3.4
2.8
2.8 |
9.0 |
3.00 |
0.35
A1 B3
|
2.8
3.1
2.9 |
8.8 |
2.93 |
0.15
A1 B4
|
3.1
2.7
3.2 |
9.0 |
3.00 |
0.26
A2 B1
|
3.0
2.6
2.5 |
8.1 |
2.70 |
0.26
A2 B2
|
3.2
3.2
2.6 |
9.0 |
3.00 |
0.35
A2 B3
|
2.8
2.8
2.9 |
8.5 |
2.83 |
0.06
A2 B4
|
3.1
3.3
3.1 |
9.5 |
3.17 |
0.12
A3 B1
|
4.0
2.8
2.3 |
9.1 |
3.03 |
0.87
A3 B2
|
3.5
2.6
2.4 |
8.5 |
2.83 |
0.59
A3 B3
|
2.7
2.9
2.9 |
8.5 |
2.83 |
0.12
A3 B4
|
2.6
2.3
2.8 |
7.7 |
2.57 |
0.25
-------------------------------------------------------------------------Total
|
|
104.1 |
2.89
-------------------------------------------------------------------------SD sampel =
0.34
B. Perhitungan nilai-nilai dispersi dari data bau asam Limbah Cair Tahu
Tabel : A X B
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
8.4
9.0
8.8
9.0 |
35.2 |
2.93 |
0.22
A2 |
8.1
9.0
8.5
9.5 |
35.1 |
2.93 |
0.27
A3 |
9.1
8.5
8.5
7.7 |
33.8 |
2.82 |
0.50
-----------------------------------------------------------------------------Total |
25.6
26.5
25.8
26.2 |
104.1
Rataan|
2.84
2.94
2.87
2.91|
SD |
0.48
0.39
0.11
0.33|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
12.1
11.3
11.8 |
35.2
A2 |
12.1
11.9
11.1 |
35.1
A3 |
12.8
10.6
10.4 |
33.8
---------------------------------------------------Total |
37.0
33.8
33.3 |
104.1
----------------------------------------------------
39
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
Total
A - B
A - r
A
B
Error
A X B
Error
=
=
=
=
=
=
a
(
(
(
(
(
=
=
b =
104.1^2 / 36 =
301.02250
2.8^2 +...+
2.8^2 )
- FK
8.4^2 +...+
7.7^2 ) / 3 - FK
12.1^2 +...+
10.4^2 ) / 4 - FK
35.2^2 +...+
33.8^2 ) / 12 - FK
25.6^2 +...+
26.2^2 ) / 9 - FK
1.2100000 - 0.1016667 =
1.1083333
0.8808333 - 0.1016667 - 0.0541667 =
4.1075000 - 1.2100000 - 0.8808333 +
=
=
=
=
=
4.1075000
0.8808333
1.2100000
0.1016667
0.0541667
0.7250000
0.1016667 =
2.1183333
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir bau Asam
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
0.1017
0.0508
0.2752
5.14
10.92
Error a
|
6
1.1083
0.1847
SD a =
0.430
KK a = 14.863 %
B
|
3
0.0542
0.0181
0.1534
3.16
5.09
A X B
|
6
0.7250
0.1208
1.0268
2.66
4.01
Error b
| 18
2.1183
0.1177
SD b =
0.343
-------------------------------------------------------------------------Total
| 35
4.1075
KK b = 11.863 %
--------------------------------------------------------------------------
40
Lampiran 10. Data Nilai akhir Bau Tanah Limbah Cair Tahu setelah Perlakuan Selama 6 Hari
A. Data Bau Tanah
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
2.7
2.3
4.0 |
9.0 |
3.00 |
0.89
A1 B2
|
3.0
0.0
0.0 |
3.0 |
1.00 |
1.73
A1 B3
|
0.0
2.5
1.5 |
4.0 |
1.33 |
1.26
A1 B4
|
2.0
2.3
2.0 |
6.3 |
2.10 |
0.17
A2 B1
|
3.0
3.1
2.5 |
8.6 |
2.87 |
0.32
A2 B2
|
3.6
3.0
1.5 |
8.1 |
2.70 |
1.08
A2 B3
|
2.0
2.8
2.8 |
7.6 |
2.53 |
0.46
A2 B4
|
2.7
3.7
3.5 |
9.9 |
3.30 |
0.53
A3 B1
|
3.3
3.3
2.4 |
9.0 |
3.00 |
0.52
A3 B2
|
3.0
2.3
1.5 |
6.8 |
2.27 |
0.75
A3 B3
|
2.0
2.8
2.3 |
7.1 |
2.37 |
0.40
A3 B4
|
0.0
2.4
2.3 |
4.7 |
1.57 |
1.36
-------------------------------------------------------------------------Total
|
|
84.1 |
2.34
-------------------------------------------------------------------------SD sampel =
1.03
B. Perhitungan nilai-nilai dispersi dari data bau tanah Limbah Cair Tahu
Tabel : A X B
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
9.0
3.0
4.0
6.3 |
22.3 |
1.86 |
1.28
A2 |
8.6
8.1
7.6
9.9 |
34.2 |
2.85 |
0.64
A3 |
9.0
6.8
7.1
4.7 |
27.6 |
2.30 |
0.89
-----------------------------------------------------------------------------Total |
26.6
17.9
18.7
20.9 |
84.1
Rataan|
2.96
1.99
2.08
2.32|
SD |
0.54
1.33
0.90
1.06|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
7.7
7.1
7.5 |
22.3
A2 |
11.3
12.6
10.3 |
34.2
A3 |
8.3
10.8
8.5 |
27.6
---------------------------------------------------Total |
27.3
30.5
26.3 |
84.1
----------------------------------------------------
41
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
84.12 / 36 =
196.46694
2.72+...+
2.32 )
- FK =
2
9.0 +...+
4.72 ) / 3 - FK =
7.72 +...+
8.52 ) / 4 - FK =
2
22.3 +...+
27.62 ) / 12 - FK =
26.62 +...+
20.92 ) / 9 - FK =
7.600556 5.923889 =
1.676667
17.123056 5.923889 5.140833 =
37.143056 7.600556 - 17.123056 +
37.143056
17.123056
7.600556
5.923889
5.140833
6.058333
5.923889 =
18.343333
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir bau tanah
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
5.9239
2.9619
10.5994 *
5.14
10.92
Error a
|
6
1.6767
0.2794
SD a =
0.529
KK a = 22.628 %
B
|
3
5.1408
1.7136
1.6815
3.16
5.09
A X B
|
6
6.0583
1.0097
0.9908
2.66
4.01
Error b
| 18
18.3433
1.0191
SD b =
1.009
-------------------------------------------------------------------------Total
| 35
37.1431
KK b = 43.212 %
--------------------------------------------------------------------------
D. Uji DMRT pengaruh perlakuan terhadap nilai akhir bau tanah
Tabel : DMRT A
Std. Error :
0.1526
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
0.53
0.55
DMRT 1%|
0.80
0.83
---------------------------------------------Perl. |
A2
A3
A1
Rataan |
2.85
2.30
1.86
---------------------------------------------A2
|
0.00
A3
|
0.55*
0.00
A1
|
0.99**
0.44
0.00
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A2
2 A3
3 A1
sesuai perlakuan
A1
1.86 b
A2
2.85 a
A3
2.30 b
2.85 a
2.30 b
1.86 b
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
42
Lampiran 11. Gambar EM4 dan Aktivitas selama penelitian
A. Gambar EM4 (Effective Microorganisms) yang digunakan dalam penelitian
B. Perlakuan Aerasi Limbah Cair Tahu
C. Hasil Pengenceran air sampel yang diinokulasikan pada media NA
43
Lampiran 1. Spesifikasi peralatan dan bahan
a. Spesifikasi alat
No.
1
Nama Alat
Derigen
Merek/Tipe
20-25 L
Kegunaan
Tempat penyimpanan
Tempat
Lab. Mikrobiologi
sementara limbah cair
tahu
2
Baskom
8 liter
Tempat perlakuan
Stasiun Percobaan
limbah cair tahu
3
Termometer
Mengukur suhu limbah
celcius
Lab. Lingkungan
cair tahu
4
pH indicator
Mengukur pH limbah
Lab. Lingkungan
5
Botol Winkler
Tempat menampung
Lab. Lingkungan
sampel untuk
pemeriksaan BOD5
6
Erlenmeyer
250 ml
Tempat menampung
Lab. Mikrobiologi
media
7
Cawan petri
Tempat membiakkan
Lab. Mikrobiologi
bakteri
8
Tabung reaksi
Tempat menampung
Lab. Mikrobiologi
akuades untuk
pengenceran sampel
limbah cair tahu
9
Mikropipet dan
1 ml
tip
11
Beker glass
Memindahkan sampel
Lab. Mikrobiologi
dengan volume kecil
1000 ml
Tempat pembuatan
Lab. Mikrobiologi
media
12
Microwave
13
Gelas ukur
250 ml
Membuat media
Lab. Mikrobiologi
Mengukur volume
Lab. Mikrobiologi
akuades
14
Autoklaf
15
Botol sampel
Sterilisasi alat dan media
100 ml
Tempat penampung
sampel limbah cair tahu
21
Lab. Mikrobiologi
Lab. Lingkungan
b. Spesifikasi bahan
1.
No
Nama Bahan
Limbah cair tahu
Spesifikasi
-
Kegunaan
Sampel
2.
EM4 (Effective
cair
Stater
-
Fermentasi EM4
microorganism)
3.
Molase
4.
KOH-KI
Mengikat oksigen
5.
MnSO4
Mengikat oksigen
6.
H2SO4
Memecah endapan
7.
Na2S2O3
8.
Larutan Buffer Fosfat
Pengatur pH
10.
Akuades
Pengenceran
11.
Media NA
Media tumbuh
0,025 N
Titrasi oksigen terlarut
mikroba
12.
Larutan phenat
Pengukuran amoniak
13.
Bunsen
Aseptis kerja
14.
Alkohol
Aseptis kerja
15.
Bubuk inhibitor
Pengukuran amoniak
nitrifikasi
22
Lampiran 2. Data Nilai akhir NH3 Limbah Cair Tahu Selama 6 Hari Perlakuan
A. Data NH3 (ppm)
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
21.8
20.9
22.4 |
65.1 |
21.70 |
0.75
A1 B2
|
18.9
18.8
19.2 |
56.9 |
18.97 |
0.21
A1 B3
|
16.6
16.2
14.8 |
47.6 |
15.87 |
0.95
A1 B4
|
14.5
14.2
12.9 |
41.6 |
13.87 |
0.85
A2 B1
|
17.7
15.9
17.0 |
50.6 |
16.87 |
0.91
A2 B2
|
14.5
12.7
13.5 |
40.7 |
13.57 |
0.90
A2 B3
|
12.5
10.4
9.8 |
32.7 |
10.90 |
1.42
A2 B4
|
9.8
7.8
6.8 |
24.4 |
8.13 |
1.53
A3 B1
|
12.8
11.2
11.5 |
35.5 |
11.83 |
0.85
A3 B2
|
8.7
8.2
9.2 |
26.1 |
8.70 |
0.50
A3 B3
|
6.2
5.8
6.7 |
18.7 |
6.23 |
0.45
A3 B4
|
4.6
4.1
3.8 |
12.5 |
4.17 |
0.40
-------------------------------------------------------------------------Total
|
|
452.4 |
12.57
-------------------------------------------------------------------------SD sampel =
5.17
B. Perhitungan nilai-nilai dispersi dari data NH3 Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
65.1
56.9
47.6
41.6 |
211.2 |
17.60 |
3.18
A2 |
50.6
40.7
32.7
24.4 |
148.4 |
12.37 |
3.53
A3 |
35.5
26.1
18.7
12.5 |
92.8 |
7.73 |
3.03
-----------------------------------------------------------------------------Total |
151.2
123.7
99.0
78.5 |
452.4
Rataan|
16.80
13.74
11.00
8.72|
SD |
4.33
4.48
4.26
4.32|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
71.8
70.1
69.3 |
211.2
A2 |
54.5
46.8
47.1 |
148.4
A3 |
32.3
29.3
31.2 |
92.8
---------------------------------------------------Total |
158.6
146.2
147.6 |
452.4
----------------------------------------------------
23
C. Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
Total
A - B
A - r
A
B
Error
A X B
Error
=
=
=
=
=
=
a
(
(
(
(
(
=
=
b =
452.42 / 36 =
5685.1600
21.82 +...+
3.82 )
- FK =
934.22000
2
65.1 +...+
12.52 ) / 3 - FK =
915.12000
71.82 +...+
31.22 ) / 4 - FK =
596.30500
211.22 +...+
92.82 ) / 12 - FK =
584.82667
151.22 +...+
78.52 ) / 9 - FK =
328.88222
596.30500 - 584.82667 =
11.47833
915.12000 - 584.82667 - 328.88222 =
1.41111
934.22000 - 596.30500 - 915.12000 + 584.82667 =
7.62167
D. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir NH 3
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
584.8267
292.4133 152.8515 **
5.14
10.92
Error a
|
6
11.4783
1.9131
SD a =
1.383
KK a = 11.006 %
B
|
3
328.8822
109.6274 258.9058 **
3.16
5.09
A X B
|
6
1.4111
0.2352
0.5554
2.66
4.01
Error b
| 18
7.6217
0.4234
SD b =
0.651
-------------------------------------------------------------------------Total
| 35
934.2200
KK b =
5.178 %
--------------------------------------------------------------------------
E. Uji DMRT pengaruh perlakuan terhadap nilai akhir NH 3
Tabel : DMRT A
Std. Error :
0.3993
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
1.38
1.43
DMRT 1%|
2.09
2.17
---------------------------------------------Perl. |
A1
A2
A3
Rataan |
17.60
12.37
7.73
---------------------------------------------A1
|
0.00
A2
|
5.23**
0.00
A3
|
9.87**
4.63**
0.00
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A1
2 A2
3 A3
sesuai perlakuan
A1
17.60 a
A2
12.37 b
A3
7.73 c
17.60 a
12.37 b
7.73 c
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
24
Tabel : DMRT B
Std. Error :
0.2169
---------------------------------------------------------Range |
2
3
4
---------------------------------------------------------P 0.05 |
2.97
3.12
3.21
P 0.01 |
4.07
4.25
4.36
DMRT 5%|
0.64
0.68
0.70
DMRT 1%|
0.88
0.92
0.95
---------------------------------------------------------Perl. |
B1
B2
B3
B4
Rataan |
16.80
13.74
11.00
8.72
---------------------------------------------------------B1
|
0.00
B2
|
3.06**
0.00
B3
|
5.80**
2.74**
0.00
B4
|
8.08**
5.02**
2.28**
0.00
---------------------------------------------------------urut rataan
1 B1
2 B2
3 B3
4 B4
16.80
13.74
11.00
8.72
sesuai perlakuan
B1
16.80 a
B2
13.74 b
B3
11.00 c
B4
8.72 d
a
b
c
d
25
Lampiran 3. Data Nilai akhir BOD5 Limbah Cair Tahu Selama 6 Hari Perlakuan
A. Tabulasi Data BOD5 (ppm)
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
| 2000.00 2190.00 2300.00 | 6490.00 | 2163.333 |
151.767
A1 B2
| 1820.00 2060.00 2220.00 | 6100.00 | 2033.333 |
201.329
A1 B3
| 2020.00 2160.00 2270.00 | 6450.00 | 2150.000 |
125.300
A1 B4
| 2040.00 2060.00 2250.00 | 6350.00 | 2116.667 |
115.902
A2 B1
| 2364.00 2404.00 2444.00 | 7212.00 | 2404.000 |
40.000
A2 B2
| 2324.00 1364.00 2284.00 | 5972.00 | 1990.667 |
543.078
A2 B3
| 2284.00 1384.00 2164.00 | 5832.00 | 1944.000 |
488.672
A2 B4
| 2244.00 1364.00 1884.00 | 5492.00 | 1830.667 |
442.418
A3 B1
| 1003.20 1001.80 1002.50 | 3007.50 | 1002.500 |
0.700
A3 B2
|
703.16
699.89
701.33 | 2104.38 |
701.460 |
1.639
A3 B3
|
385.15
384.62
386.04 | 1155.81 |
385.270 |
0.718
A3 B4
|
139.23
140.05
139.88 |
419.16 |
139.720 |
0.433
-------------------------------------------------------------------------Total
|
| 56584.85 | 1571.801
-------------------------------------------------------------------------SD sampel =
794.111
B. Perhitungan nilai-nilai disperse dari data BOD5 Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 | 6490.00 6100.00 6450.00 6350.00 | 25390.00 | 2115.833 |
140.160
A2 | 7212.00 5972.00 5832.00 5492.00 | 24508.00 | 2042.333 |
429.161
A3 | 3007.50 2104.38 1155.81
419.16 | 6686.85 |
557.238 |
339.634
-----------------------------------------------------------------------------Total | 16709.50 14176.38 13437.81 12261.16 | 56584.85
Rataan| 1856.611 1575.153 1493.090 1362.351|
SD |
653.732 716.650 872.879 953.136|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 | 7880.00 8470.00 9040.00 | 25390.00
A2 | 9216.00 6516.00 8776.00 | 24508.00
A3 | 2230.74 2226.36 2229.75 | 6686.85
---------------------------------------------------Total | 19326.74 17212.36 20045.75 | 56584.85
----------------------------------------------------
26
C. Perhitungan Jumlah Kuadrat BOD5
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
Total
A - B
A - r
A
B
Error
A X B
Error
56584.852 / 36 =
88940146
( 2000.002 +...+
139.882 )
( 6490.002 +...+
419.162 )
2
( 7880.00 +...+
2229.752 )
( 25390.002 +...+
6686.852 )
( 16709.502 +...+ 12261.162 )
=
19778017 18560531 =
=
20423914 18560531 b =
22071455 19778017 =
=
=
=
=
=
a
- FK =
/ 3 - FK =
/ 4 - FK =
/ 12 - FK =
/ 9 - FK =
1217486
1180734 =
20423914 +
22071455
20423914
19778017
18560531
1180734
682648
18560531 =
430055
D. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir BOD5
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2 18560531.4235 9280265.7117
45.7349 **
5.14
10.92
Error a
|
6 1217485.9714
202914.3286
SD a = 450.460
KK a = 28.659 %
B
|
3 1180734.4351
393578.1450
16.4733 **
3.16
5.09
A X B
|
6
682648.0868
113774.6811
4.7621 **
2.66
4.01
Error b
| 18
430055.1181
23891.9510
SD b = 154.570
-------------------------------------------------------------------------Total
| 35 22071455.0348
KK b =
9.834 %
--------------------------------------------------------------------------
E. Uji DMRT pengaruh perlakuan terhadap nilai akhir BOD5
Tabel : DMRT A
Std. Error :
130.0366
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
450.06
466.44
DMRT 1%|
681.78
707.27
---------------------------------------------Perl. |
A1
A2
A3
Rataan | 2115.833
2042.333
557.238
---------------------------------------------A1
|
0.000
A2
|
73.500
0.000
A3
| 1558.596** 1485.096**
0.000
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A1
2115.833 a
2 A2
2042.333 a
3 A3
557.238 b
sesuai perlakuan
A1
2115.833 a
A2
2042.333 a
A3
557.238 b
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
27
Tabel : DMRT B
Std. Error :
51.5234
---------------------------------------------------------Range |
2
3
4
---------------------------------------------------------P 0.05 |
2.97
3.12
3.21
P 0.01 |
4.07
4.25
4.36
DMRT 5%|
153.08
160.65
165.39
DMRT 1%|
209.75
218.77
224.75
---------------------------------------------------------Perl. |
B1
B2
B3
B4
Rataan | 1856.611
1575.153
1493.090
1362.351
---------------------------------------------------------B1
|
0.000
B2
|
281.458**
0.000
B3
|
363.521**
82.063
0.000
B4
|
494.260**
212.802*
130.739
0.000
---------------------------------------------------------Pemberian tanda pembeda
urut rataan
1 B1
2 B2
3 B3
4 B4
1856.611
1575.153
1493.090
1362.351
sesuai perlakuan
B1
1856.611 a
B2
1575.153 b
B3
1493.090 bc
B4
1362.351 c
a
b
bc
c
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
Tabel : DMRT A x B
Std. Error :
151.2697
--------------------------------------------------------------------------------------------------------------------------------------------------------Range |
2
3
4
5
6
7
8
9
10
11
12
--------------------------------------------------------------------------------------------------------------------------------------------------------P 0.05 |
2.97
3.12
3.21
3.27
3.32
3.36
3.38
3.41
3.42
3.44
3.45
P 0.01 |
4.07
4.25
4.36
4.45
4.51
4.56
4.60
4.64
4.66
4.69
4.71
DMRT 5%|
449.42
471.66
485.58
495.26
502.37
507.66
511.75
515.07
517.49
519.61
521.12
DMRT 1%|
615.82
642.29
659.84
672.39
682.07
689.79
695.99
701.13
705.52
709.30
712.63
--------------------------------------------------------------------------------------------------------------------------------------------------------Perl. |
A2B1
A1B1
A1B3
A1B4
A1B2
A2B2
A2B3
A2B4
A3B1
A3B2
A3B3
A3B4
Rataan | 2404.000
2163.333
2150.000
2116.667
2033.333
1990.667
1944.000
1830.667
1002.500
701.460
385.270
139.720
--------------------------------------------------------------------------------------------------------------------------------------------------------A2B1 |
0.000
A1B1 |
240.667
0.000
A1B3 |
254.000
13.333
0.000
A1B4 |
287.333
46.667
33.333
0.000
A1B2 |
370.667
130.000
116.667
83.333
0.000
A2B2 |
413.333
172.667
159.333
126.000
42.667
0.000
A2B3 |
460.000
219.333
206.000
172.667
89.333
46.667
0.000
A2B4 |
573.333*
332.667
319.333
286.000
202.667
160.000
113.333
0.000
A3B1 | 1401.500** 1160.833** 1147.500** 1114.167** 1030.833**
988.167**
941.500**
828.167**
0.000
A3B2 | 1702.540** 1461.873** 1448.540** 1415.207** 1331.873** 1289.207** 1242.540** 1129.207**
301.040
0.000
A3B3 | 2018.730** 1778.063** 1764.730** 1731.397** 1648.063** 1605.397** 1558.730** 1445.397**
617.230*
316.190
0.000
A3B4 | 2264.280** 2023.613** 2010.280** 1976.947** 1893.613** 1850.947** 1804.280** 1690.947**
862.780**
561.740*
245.550
0.000
---------------------------------------------------------------------------------------------------------------------------------------------------------
1
2
3
4
5
6
7
8
9
10
11
12
urut rataan
A2B1
2404.000
A1B1
2163.333
A1B3
2150.000
A1B4
2116.667
A1B2
2033.333
A2B2
1990.667
A2B3
1944.000
A2B4
1830.667
A3B1
1002.500
A3B2
701.460
A3B3
385.270
A3B4
139.720
sesuai perlakuan
A1B1
2163.333 ab
A1B2
2033.333 ab
A1B3
2150.000 ab
A1B4
2116.667 ab
A2B1
2404.000 a
A2B2
1990.667 ab
A2B3
1944.000 ab
A2B4
1830.667 b
A3B1
1002.500 c
A3B2
701.460 cd
A3B3
385.270 de
A3B4
139.720 e
a
ab
ab
ab
ab
ab
ab
b
c
cd
de
e
28
Lampiran 4. Data Nilai akhir pH Limbah Cair Tahu Selama 6 Hari Perlakuan
A.Tabulasi Data pH
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
4
4
4 |
12 |
4.0 |
0.0
A1 B2
|
4
4
4 |
12 |
4.0 |
0.0
A1 B3
|
4
4
4 |
12 |
4.0 |
0.0
A1 B4
|
4
4
4 |
12 |
4.0 |
0.0
A2 B1
|
5
4
4 |
13 |
4.3 |
0.6
A2 B2
|
5
4
5 |
14 |
4.7 |
0.6
A2 B3
|
4
5
5 |
14 |
4.7 |
0.6
A2 B4
|
4
5
4 |
13 |
4.3 |
0.6
A3 B1
|
8
6
7 |
21 |
7.0 |
1.0
A3 B2
|
5
7
5 |
17 |
5.7 |
1.2
A3 B3
|
8
5
8 |
21 |
7.0 |
1.7
A3 B4
|
5
5
5 |
15 |
5.0 |
0.0
-------------------------------------------------------------------------Total
|
|
176 |
4.9
-------------------------------------------------------------------------SD sampel =
1.2
B. Perhitungan nilai-nilai dispersi dari data pH Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
12
12
12
12 |
48 |
4.0 |
0.0
A2 |
13
14
14
13 |
54 |
4.5 |
0.5
A3 |
21
17
21
15 |
74 |
6.2 |
1.3
-----------------------------------------------------------------------------Total |
46
43
47
40 |
176
Rataan|
5.1
4.8
5.2
4.4|
SD |
1.5
1.0
1.6
0.5|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
16
16
16 |
48
A2 |
18
18
18 |
54
A3 |
26
23
25 |
74
---------------------------------------------------Total |
60
57
59 |
176
----------------------------------------------------
29
C. Perhitungan Jumlah Kuadrat pH
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
Total
A - B
A - r
A
B
Error
A X B
Error
=
=
=
=
=
=
a
(
(
(
(
(
=
=
b =
176^2 / 36 =
860.44444
42 +...+
52 )
- FK =
53.555556
122 +...+
152 ) / 3 - FK =
40.222222
162 +...+
252 ) / 4 - FK =
32.055556
482 +...+
742 ) / 12 - FK =
30.888889
462 +...+
402 ) / 9 - FK =
3.333333
32.055556 - 30.888889 =
1.166667
40.222222 - 30.888889 3.333333 =
6.000000
53.555556 - 32.055556 - 40.222222 + 30.888889 =
12.166667
D. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir pH
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
30.8889
15.4444
79.4286 **
5.14
10.92
Error a
|
6
1.1667
0.1944
SD a =
0.441
KK a =
9.020 %
B
|
3
3.3333
1.1111
1.6438
3.16
5.09
A X B
|
6
6.0000
1.0000
1.4795
2.66
4.01
Error b
| 18
12.1667
0.6759
SD b =
0.822
-------------------------------------------------------------------------Total
| 35
53.5556
KK b = 16.817 %
--------------------------------------------------------------------------
E. Uji DMRT pengaruh perlakuan terhadap nilai akhir pH
Tabel : DMRT A
Std. Error :
0.1273
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
0.44
0.46
DMRT 1%|
0.67
0.69
---------------------------------------------Perl. |
A3
A2
A1
Rataan |
6.2
4.5
4.0
---------------------------------------------A3
|
0.0
A2
|
1.7**
0.0
A1
|
2.2**
0.5*
0.0
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A3
2 A2
3 A1
sesuai perlakuan
A1
4.0 c
A2
4.5 b
A3
6.2 a
6.2 a
4.5 b
4.0 c
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
30
Lampiran 5. Data Nilai akhir Suhu Limbah Cair Tahu Selama 6 Hari Perlakuan
A. Data Suhu OC
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
27
27
27 |
81 |
27.0 |
0.0
A1 B2
|
27
27
27 |
81 |
27.0 |
0.0
A1 B3
|
27
27
27 |
81 |
27.0 |
0.0
A1 B4
|
27
27
27 |
81 |
27.0 |
0.0
A2 B1
|
27
26
26 |
79 |
26.3 |
0.6
A2 B2
|
26
27
26 |
79 |
26.3 |
0.6
A2 B3
|
27
27
26 |
80 |
26.7 |
0.6
A2 B4
|
26
28
26 |
80 |
26.7 |
1.2
A3 B1
|
28
28
28 |
84 |
28.0 |
0.0
A3 B2
|
28
28
28 |
84 |
28.0 |
0.0
A3 B3
|
28
28
28 |
84 |
28.0 |
0.0
A3 B4
|
28
28
28 |
84 |
28.0 |
0.0
-------------------------------------------------------------------------Total
|
|
978 |
27.2
-------------------------------------------------------------------------SD sampel =
0.7
B. Perhitungan nilai-nilai dispersi dari data suhu Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
81
81
81
81 |
324 |
27.0 |
0.0
A2 |
79
79
80
80 |
318 |
26.5 |
0.7
A3 |
84
84
84
84 |
336 |
28.0 |
0.0
-----------------------------------------------------------------------------Total |
244
244
245
245 |
978
Rataan|
27.1
27.1
27.2
27.2|
SD |
0.8
0.8
0.7
0.8|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
108
108
108 |
324
A2 |
106
108
104 |
318
A3 |
112
112
112 |
336
---------------------------------------------------Total |
326
328
324 |
978
----------------------------------------------------
31
C. Perhitungan Jumlah Kuadrat Suhu
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
9782/ 36 =
26569.000
272 +...+
282 )
- FK =
19.000000
812 +...+
842 ) / 3 - FK =
14.333333
1082 +...+
1122) / 4 - FK =
16.000000
3242 +...+
3362 ) / 12 - FK =
14.000000
2442 +...+
2452 ) / 9 - FK =
0.111111
16.000000 - 14.000000 =
2.000000
14.333333 - 14.000000 0.111111 =
0.222222
19.000000 - 16.000000 - 14.333333 + 14.000000 =
2.666667
D. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir suhu
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
14.0000
7.0000
21.0000 **
5.14
10.92
Error a
|
6
2.0000
0.3333
SD a =
0.577
KK a =
2.125 %
B
|
3
0.1111
0.0370
0.2500
3.16
5.09
A X B
|
6
0.2222
0.0370
0.2500
2.66
4.01
Error b
| 18
2.6667
0.1481
SD b =
0.385
-------------------------------------------------------------------------Total
| 35
19.0000
KK b =
1.417 %
--------------------------------------------------------------------------
E. Uji DMRT pengaruh perlakuan terhadap nilai akhir suhu
Tabel : DMRT A
Std. Error :
0.1667
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
0.58
0.60
DMRT 1%|
0.87
0.91
---------------------------------------------Perl. |
A3
A1
A2
Rataan |
28.0
27.0
26.5
---------------------------------------------A3
|
0.0
A1
|
1.0**
0.0
A2
|
1.5**
0.5
0.0
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A3
2 A1
3 A2
sesuai perlakuan
A1
27.0 b
A2
26.5 b
A3
28.0 a
28.0 a
27.0 b
26.5 b
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
32
Lampiran 6. Data Nilai akhir Jumlah Mikroba Limbah Cair Tahu setelah Perlakuan Selama 6
Hari
Data : Jumlah mikroba
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
8.778
7.301
9.602 |
25.681 |
8.5603 |
1.1658
A1 B2
|
9.602
8.000
9.602 |
27.204 |
9.0680 |
0.9249
A1 B3
|
9.505
8.699
9.602 |
27.806 |
9.2687 |
0.4957
A1 B4
|
8.204
8.301
9.602 |
26.107 |
8.7023 |
0.7806
A2 B1
|
8.699
8.447
8.477 |
25.623 |
8.5410 |
0.1377
A2 B2
|
7.477
7.477
7.477 |
22.431 |
7.4770 |
0.0000
A2 B3
|
8.146
8.000
8.146 |
24.292 |
8.0973 |
0.0843
A2 B4
|
7.845
8.699
8.079 |
24.623 |
8.2077 |
0.4413
A3 B1
|
8.398
8.176
8.279 |
24.853 |
8.2843 |
0.1111
A3 B2
|
8.602
8.204
7.477 |
24.283 |
8.0943 |
0.5705
A3 B3
|
9.380
8.000
8.342 |
25.722 |
8.5740 |
0.7187
A3 B4
|
8.954
7.699
7.903 |
24.556 |
8.1853 |
0.6735
-------------------------------------------------------------------------Total
|
| 303.181 |
8.4217
-------------------------------------------------------------------------SD sampel =
0.6904
B. Perhitungan nilai-nilai dispersi dari data jumlah mikroba Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
25.681
27.204
27.806
26.107 | 106.798 |
8.8998 |
0.8031
A2 |
25.623
22.431
24.292
24.623 |
96.969 |
8.0808 |
0.4492
A3 |
24.853
24.283
25.722
24.556 |
99.414 |
8.2845 |
0.5227
-----------------------------------------------------------------------------Total |
76.157
73.918
77.820
75.286 | 303.181
Rataan|
8.4619
8.2131
8.6467
8.3651|
SD |
0.6045
0.8819
0.6727
0.6152|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
36.089
32.301
38.408 | 106.798
A2 |
32.167
32.623
32.179 |
96.969
A3 |
35.334
32.079
32.001 |
99.414
---------------------------------------------------Total | 103.590
97.003 102.588 | 303.181
----------------------------------------------------
33
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
303.1812 / 36 =
2553.2977
8.7782 +...+
7.9032 )
- FK =
16.684290
2
25.681 +...+
24.5562) / 3 - FK =
7.487544
36.0892 +...+
32.0012 ) / 4 - FK =
10.958967
2
106.798 +...+
99.4142 ) / 12 - FK =
4.364187
76.1572 +...+
75.286^2 ) / 9 - FK =
0.890431
10.958967 4.364187 =
6.594781
7.487544 4.364187 0.890431 =
2.232927
16.684290 - 10.958967 7.487544 +
4.364187 =
2.601965
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir jumlah
mikroba limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
4.3642
2.1821
1.9853
5.14
10.92
Error a
|
6
6.5948
1.0991
SD a =
1.048
KK a = 12.449 %
B
|
3
0.8904
0.2968
2.0533
3.16
5.09
A X B
|
6
2.2329
0.3722
2.5745
2.66
4.01
Error b
| 18
2.6020
0.1446
SD b =
0.380
-------------------------------------------------------------------------Total
| 35
16.6843
KK b =
4.515 %
--------------------------------------------------------------------------
34
Lampiran 7. Data Nilai akhir Jumlah Bau Amoniak Cair Tahu setelah Perlakuan Selama 6 Hari
A. Data Bau Amoniak
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
2.0
2.4
1.8 |
6.2 |
2.07 |
0.31
A1 B2
|
2.3
2.8
2.0 |
7.1 |
2.37 |
0.40
A1 B3
|
2.0
2.3
3.4 |
7.7 |
2.57 |
0.74
A1 B4
|
3.0
2.0
2.3 |
7.3 |
2.43 |
0.51
A2 B1
|
4.0
3.0
2.2 |
9.2 |
3.07 |
0.90
A2 B2
|
2.5
3.3
1.8 |
7.6 |
2.53 |
0.75
A2 B3
|
2.8
3.0
3.0 |
8.8 |
2.93 |
0.12
A2 B4
|
3.0
3.5
4.0 |
10.5 |
3.50 |
0.50
A3 B1
|
3.0
3.2
1.6 |
7.8 |
2.60 |
0.87
A3 B2
|
3.2
2.8
2.6 |
8.6 |
2.87 |
0.31
A3 B3
|
2.8
3.0
2.0 |
7.8 |
2.60 |
0.53
A3 B4
|
3.1
1.6
3.1 |
7.8 |
2.60 |
0.87
-------------------------------------------------------------------------Total
|
|
96.4 |
2.68
-------------------------------------------------------------------------SD sampel =
0.63
B. Perhitungan nilai-nilai dispersi dari data bau amoniak Limbah Cair Tahu
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
6.2
7.1
7.7
7.3 |
28.3 |
2.36 |
0.48
A2 |
9.2
7.6
8.8
10.5 |
36.1 |
3.01 |
0.65
A3 |
7.8
8.6
7.8
7.8 |
32.0 |
2.67 |
0.60
-----------------------------------------------------------------------------Total |
23.2
23.3
24.3
25.6 |
96.4
Rataan|
2.58
2.59
2.70
2.84|
SD |
0.78
0.50
0.49
0.75|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
9.3
9.5
9.5 |
28.3
A2 |
12.3
12.8
11.0 |
36.1
A3 |
12.1
10.6
9.3 |
32.0
---------------------------------------------------Total |
33.7
32.9
29.8 |
96.4
----------------------------------------------------
35
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
96.42 / 36 =
258.13778
2.02 +...+
3.12 )
- FK =
2
6.2 +...+
7.82) / 3 - FK =
9.32 +...+
9.32 ) / 4 - FK =
2
28.3 +...+
32.02 ) / 12 - FK =
23.22 +...+
25.62 ) / 9 - FK =
3.957222 2.537222 =
1.420000
4.528889 2.537222 0.415556 =
13.702222 3.957222 4.528889 +
13.702222
4.528889
3.957222
2.537222
0.415556
1.576111
2.537222 =
7.753333
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir bau
amoniak limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
2.5372
1.2686
5.3603 *
5.14
10.92
Error a
|
6
1.4200
0.2367
SD a =
0.486
KK a = 18.167 %
B
|
3
0.4156
0.1385
0.3216
3.16
5.09
A X B
|
6
1.5761
0.2627
0.6098
2.66
4.01
Error b
| 18
7.7533
0.4307
SD b =
0.656
-------------------------------------------------------------------------Total
| 35
13.7022
KK b = 24.509 %
--------------------------------------------------------------------------
D. Uji DMRT pengaruh perlakuan terhadap nilai akhir bau amoniak
Tabel : DMRT A
Std. Error :
0.1404
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
0.49
0.50
DMRT 1%|
0.74
0.76
---------------------------------------------Perl. |
A2
A3
A1
Rataan |
3.01
2.67
2.36
---------------------------------------------A2
|
0.00
A3
|
0.34
0.00
A1
|
0.65*
0.31
0.00
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A2
2 A3
3 A1
sesuai perlakuan
A1
2.36 b
A2
3.01 a
A3
2.67 ab
3.01 a
2.67 ab
2.36 b
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
36
Lampiran 8. Data Nilai akhir Bau H2S Limbah Cair Tahu setelah Perlakuan Selama 6 Hari
A. Data Bau H2S
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
3.2
2.9
3.4 |
9.5 |
3.17 |
0.25
A1 B2
|
3.1
3.4
3.1 |
9.6 |
3.20 |
0.17
A1 B3
|
2.4
3.8
2.8 |
9.0 |
3.00 |
0.72
A1 B4
|
3.0
2.6
3.0 |
8.6 |
2.87 |
0.23
A2 B1
|
3.3
3.3
3.3 |
9.9 |
3.30 |
0.00
A2 B2
|
3.3
3.3
3.1 |
9.7 |
3.23 |
0.12
A2 B3
|
3.6
3.3
3.8 |
10.7 |
3.57 |
0.25
A2 B4
|
2.7
3.4
3.7 |
9.8 |
3.27 |
0.51
A3 B1
|
3.4
3.5
2.9 |
9.8 |
3.27 |
0.32
A3 B2
|
3.7
3.5
2.9 |
10.1 |
3.37 |
0.42
A3 B3
|
3.5
3.4
3.5 |
10.4 |
3.47 |
0.06
A3 B4
|
3.6
2.9
3.5 |
10.0 |
3.33 |
0.38
-------------------------------------------------------------------------Total
|
|
117.1 |
3.25
-------------------------------------------------------------------------SD sampel =
0.34
B. Perhitungan nilai-nilai dispersi dari data bau H2S Limbah Cair Tahu
Tabel : A X B
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
9.5
9.6
9.0
8.6 |
36.7 |
3.06 |
0.38
A2 |
9.9
9.7
10.7
9.8 |
40.1 |
3.34 |
0.28
A3 |
9.8
10.1
10.4
10.0 |
40.3 |
3.36 |
0.29
-----------------------------------------------------------------------------Total |
29.2
29.4
30.1
28.4 |
117.1
Rataan|
3.24
3.27
3.34
3.16|
SD |
0.21
0.24
0.46
0.40|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
11.7
12.7
12.3 |
36.7
A2 |
12.9
13.3
13.9 |
40.1
A3 |
14.2
13.3
12.8 |
40.3
---------------------------------------------------Total |
38.8
39.3
39.0 |
117.1
----------------------------------------------------
37
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
117.12 / 36 =
380.90028
3.22 +...+
3.52 )
- FK =
4.0297222
2
9.5 +...+
10.02 ) / 3 - FK =
1.1697222
11.72 +...+
12.82 ) / 4 - FK =
1.1872222
2
36.7 +...+
40.32 ) / 12 - FK =
0.6822222
29.22 +...+
28.42 ) / 9 - FK =
0.1630556
1.1872222 - 0.6822222 =
0.5050000
1.1697222 - 0.6822222 - 0.1630556 =
0.3244444
4.0297222 - 1.1872222 - 1.1697222 + 0.6822222 =
2.3550000
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir bau H2S
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
0.6822
0.3411
4.0528
5.14
10.92
Error a
|
6
0.5050
0.0842
SD a =
0.290
KK a =
8.919 %
B
|
3
0.1631
0.0544
0.4154
3.16
5.09
A X B
|
6
0.3244
0.0541
0.4133
2.66
4.01
Error b
| 18
2.3550
0.1308
SD b =
0.362
-------------------------------------------------------------------------Total
| 35
4.0297
KK b = 11.120 %
--------------------------------------------------------------------------
38
Lampiran 9. Data Nilai akhir Bau Asam Limbah Cair Tahu setelah Perlakuan Selama 6 Hari
A. Data Bau Asam
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
2.8
2.7
2.9 |
8.4 |
2.80 |
0.10
A1 B2
|
3.4
2.8
2.8 |
9.0 |
3.00 |
0.35
A1 B3
|
2.8
3.1
2.9 |
8.8 |
2.93 |
0.15
A1 B4
|
3.1
2.7
3.2 |
9.0 |
3.00 |
0.26
A2 B1
|
3.0
2.6
2.5 |
8.1 |
2.70 |
0.26
A2 B2
|
3.2
3.2
2.6 |
9.0 |
3.00 |
0.35
A2 B3
|
2.8
2.8
2.9 |
8.5 |
2.83 |
0.06
A2 B4
|
3.1
3.3
3.1 |
9.5 |
3.17 |
0.12
A3 B1
|
4.0
2.8
2.3 |
9.1 |
3.03 |
0.87
A3 B2
|
3.5
2.6
2.4 |
8.5 |
2.83 |
0.59
A3 B3
|
2.7
2.9
2.9 |
8.5 |
2.83 |
0.12
A3 B4
|
2.6
2.3
2.8 |
7.7 |
2.57 |
0.25
-------------------------------------------------------------------------Total
|
|
104.1 |
2.89
-------------------------------------------------------------------------SD sampel =
0.34
B. Perhitungan nilai-nilai dispersi dari data bau asam Limbah Cair Tahu
Tabel : A X B
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
8.4
9.0
8.8
9.0 |
35.2 |
2.93 |
0.22
A2 |
8.1
9.0
8.5
9.5 |
35.1 |
2.93 |
0.27
A3 |
9.1
8.5
8.5
7.7 |
33.8 |
2.82 |
0.50
-----------------------------------------------------------------------------Total |
25.6
26.5
25.8
26.2 |
104.1
Rataan|
2.84
2.94
2.87
2.91|
SD |
0.48
0.39
0.11
0.33|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
12.1
11.3
11.8 |
35.2
A2 |
12.1
11.9
11.1 |
35.1
A3 |
12.8
10.6
10.4 |
33.8
---------------------------------------------------Total |
37.0
33.8
33.3 |
104.1
----------------------------------------------------
39
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
Total
A - B
A - r
A
B
Error
A X B
Error
=
=
=
=
=
=
a
(
(
(
(
(
=
=
b =
104.1^2 / 36 =
301.02250
2.8^2 +...+
2.8^2 )
- FK
8.4^2 +...+
7.7^2 ) / 3 - FK
12.1^2 +...+
10.4^2 ) / 4 - FK
35.2^2 +...+
33.8^2 ) / 12 - FK
25.6^2 +...+
26.2^2 ) / 9 - FK
1.2100000 - 0.1016667 =
1.1083333
0.8808333 - 0.1016667 - 0.0541667 =
4.1075000 - 1.2100000 - 0.8808333 +
=
=
=
=
=
4.1075000
0.8808333
1.2100000
0.1016667
0.0541667
0.7250000
0.1016667 =
2.1183333
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir bau Asam
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
0.1017
0.0508
0.2752
5.14
10.92
Error a
|
6
1.1083
0.1847
SD a =
0.430
KK a = 14.863 %
B
|
3
0.0542
0.0181
0.1534
3.16
5.09
A X B
|
6
0.7250
0.1208
1.0268
2.66
4.01
Error b
| 18
2.1183
0.1177
SD b =
0.343
-------------------------------------------------------------------------Total
| 35
4.1075
KK b = 11.863 %
--------------------------------------------------------------------------
40
Lampiran 10. Data Nilai akhir Bau Tanah Limbah Cair Tahu setelah Perlakuan Selama 6 Hari
A. Data Bau Tanah
Tabulasi data
-------------------------------------------------------------------------Kombinasi |
Ulangan
| Total
| Rataan
| Standar
Perlakuan |
1
2
3
|
|
| Deviasi
-------------------------------------------------------------------------A1 B1
|
2.7
2.3
4.0 |
9.0 |
3.00 |
0.89
A1 B2
|
3.0
0.0
0.0 |
3.0 |
1.00 |
1.73
A1 B3
|
0.0
2.5
1.5 |
4.0 |
1.33 |
1.26
A1 B4
|
2.0
2.3
2.0 |
6.3 |
2.10 |
0.17
A2 B1
|
3.0
3.1
2.5 |
8.6 |
2.87 |
0.32
A2 B2
|
3.6
3.0
1.5 |
8.1 |
2.70 |
1.08
A2 B3
|
2.0
2.8
2.8 |
7.6 |
2.53 |
0.46
A2 B4
|
2.7
3.7
3.5 |
9.9 |
3.30 |
0.53
A3 B1
|
3.3
3.3
2.4 |
9.0 |
3.00 |
0.52
A3 B2
|
3.0
2.3
1.5 |
6.8 |
2.27 |
0.75
A3 B3
|
2.0
2.8
2.3 |
7.1 |
2.37 |
0.40
A3 B4
|
0.0
2.4
2.3 |
4.7 |
1.57 |
1.36
-------------------------------------------------------------------------Total
|
|
84.1 |
2.34
-------------------------------------------------------------------------SD sampel =
1.03
B. Perhitungan nilai-nilai dispersi dari data bau tanah Limbah Cair Tahu
Tabel : A X B
Tabel : A X B
-----------------------------------------------------------------------------|
B1
B2
B3
B4
| Total
| Rataan
|
SD
-----------------------------------------------------------------------------A1 |
9.0
3.0
4.0
6.3 |
22.3 |
1.86 |
1.28
A2 |
8.6
8.1
7.6
9.9 |
34.2 |
2.85 |
0.64
A3 |
9.0
6.8
7.1
4.7 |
27.6 |
2.30 |
0.89
-----------------------------------------------------------------------------Total |
26.6
17.9
18.7
20.9 |
84.1
Rataan|
2.96
1.99
2.08
2.32|
SD |
0.54
1.33
0.90
1.06|
------------------------------------------------------------------------------
Tabel : A X r
---------------------------------------------------|
r1
r2
r3
| TOTAL
---------------------------------------------------A1 |
7.7
7.1
7.5 |
22.3
A2 |
11.3
12.6
10.3 |
34.2
A3 |
8.3
10.8
8.5 |
27.6
---------------------------------------------------Total |
27.3
30.5
26.3 |
84.1
----------------------------------------------------
41
Perhitungan jumlah kuadrat (JK)
FK
JK
JK
JK
JK
JK
JK
JK
JK
=
=
=
=
=
=
a
Total
A - B
A - r
A
B
Error
A X B
Error b
(
(
(
(
(
=
=
=
84.12 / 36 =
196.46694
2.72+...+
2.32 )
- FK =
2
9.0 +...+
4.72 ) / 3 - FK =
7.72 +...+
8.52 ) / 4 - FK =
2
22.3 +...+
27.62 ) / 12 - FK =
26.62 +...+
20.92 ) / 9 - FK =
7.600556 5.923889 =
1.676667
17.123056 5.923889 5.140833 =
37.143056 7.600556 - 17.123056 +
37.143056
17.123056
7.600556
5.923889
5.140833
6.058333
5.923889 =
18.343333
C. Analisis variansi pengaruh lama aerasi dan konsentrasi EM4 terhadap nilai akhir bau tanah
limbah cair tahu setelah perlakuan selama 6 hari
Tabel analisis variansi
-------------------------------------------------------------------------Sumber
| Derajat Jumlah
Kuadrat
F
F Tabel
Variasi | Bebas
Kuadrat
Tengah
Hitung
0.05
0.01
-------------------------------------------------------------------------A
|
2
5.9239
2.9619
10.5994 *
5.14
10.92
Error a
|
6
1.6767
0.2794
SD a =
0.529
KK a = 22.628 %
B
|
3
5.1408
1.7136
1.6815
3.16
5.09
A X B
|
6
6.0583
1.0097
0.9908
2.66
4.01
Error b
| 18
18.3433
1.0191
SD b =
1.009
-------------------------------------------------------------------------Total
| 35
37.1431
KK b = 43.212 %
--------------------------------------------------------------------------
D. Uji DMRT pengaruh perlakuan terhadap nilai akhir bau tanah
Tabel : DMRT A
Std. Error :
0.1526
---------------------------------------------Range |
2
3
---------------------------------------------P 0.05 |
3.46
3.59
P 0.01 |
5.24
5.44
DMRT 5%|
0.53
0.55
DMRT 1%|
0.80
0.83
---------------------------------------------Perl. |
A2
A3
A1
Rataan |
2.85
2.30
1.86
---------------------------------------------A2
|
0.00
A3
|
0.55*
0.00
A1
|
0.99**
0.44
0.00
---------------------------------------------Pemberian tanda pembeda
urut rataan
1 A2
2 A3
3 A1
sesuai perlakuan
A1
1.86 b
A2
2.85 a
A3
2.30 b
2.85 a
2.30 b
1.86 b
Angka yang diikuti huruf yang sama tidak berbeda nyata pada DMRT 5%
42
Lampiran 11. Gambar EM4 dan Aktivitas selama penelitian
A. Gambar EM4 (Effective Microorganisms) yang digunakan dalam penelitian
B. Perlakuan Aerasi Limbah Cair Tahu
C. Hasil Pengenceran air sampel yang diinokulasikan pada media NA
43