0,05 mgL Gula 30 gL bacto agar 9 gL arang Aktif 2 gL pH 6

  LAMPIRAN Lampiran 1. Peta Alir Penelitian

  Lampiran 2 . Komposisi Medium Y3 Makro elemen Satuan

NH4CL 535 mg/L

KNO3 2020 mg/L

  MgSO4.7H2O 247 mg/L CaCL2. 2H2O 294 mg/L

KCL 1492 mg/L

NaH2PO4 312 mg/L

  Mikro elemen Satuan KJ 8,3 mg/L

H3BO3 3,1 mg/L

  MnSO4 .4H2O 11,2 mg/L ZnSO4. 7H2O 7,2 mg/L CuSO4. 5H2O 0,25 mg/L CoCL2. 6H2O 0,24 mg/L

  NaMoO4. H2O 0,24 mg/L NiCL2. 6H2O 0,024 mg/L

Iron Satuan

  Fe2SO4. 7H2O 41,7 mg/L

Na2EDTA 53,8 mg/L

Vitamin Satuan

  

Piridoxin HCL 0,05 mg/L

Tiamin HCL 0,05 mg/L

Gula 30 g/L

bacto agar 9 g/L

arang Aktif 2 g/L

  Lampiran 3 . Karekteristik Embrio Somatik secara Morfologi dan Histologi

A. Karakteristik Embrio Somatik secara Morfologi

  Karakteristik Tahapan Perkembangan Embrio Somatik Globular Skutelar Berbentuk Hati

  Kotiledon Normal Bulat Simetris Monokotiledon Bipolar

  Permukaan rata

  B. karakterisasi Embrio Somatik Secara Histologi Karakteristik Tahapan Perkembangan Embrio Somatik

  Globular Skutelar Berbentuk Hati Kotiledon Normal Meristematik Ada beberapa sel Menyebar merata Menyebar merata meristematik inti sel Inti sel tampak jelas tampak jelas Jaringan

  Tidak bercabang Tidak bercabang Tidak bercabang

  Prokambial ( teratur ) ( teratur ) Protoderm Satu lapis, rapi Satu lapis, rapi Satu lapis, rapi dan terlihat jelas dan terlihat jelas dan terlihat jelas

  Lampiran 4. Data Pengamatan Waktu Terbentuknya Kalus Primer PERLAKUAN ULANGAN RATA- RATA

  6 35,3% A1M

  1

  1

  4 23,5% A3B

  1

  1

  1

  1

  1

  1

  1

  1

  1 5,9% A2M

  1

  1 5,9% A3M

  1

  1 5,9% A1A 0% A2A

  1

  1 5,9% A3A

  1

  1 5,9% Total

  1

  2 11,7% A2B

  I II III

  68

  IV V VI VII VIII 110 mg/L

A1A - - - 102 - - - - 102

  A1M - - 95 - 97 - - -

  96 A1B 68 72 - 72 - 75 - -

  71 120 mg/L A2A - 87 85 - - - - -

  86 A2M - - - -- - -

  82

  82

  82 A2B 70 63 65 - -

  68

  70

  1

  67.3 130 mg/L A3A 73 - - - - - - -

  73 A3M - - - - 68 65 - -

  65 A3B 57 57 62 - 59 - 62 -

  59.4 Lampiran 5. Data Persentase Konsentrasi 2,4-D dengan Posisi Eksplan Terhadap Induksi Kalus Primer Perlakuan ulangan Total Persentase

  I II

  III

  IV V

  VI VII VIII A1B

  1

  17 100%

  Lampiran 6. Data Pengamatan Waktu Terbentuknya Kalus Embriogenik PERLAKUAN ULANGAN RATA- RATA

  I II

  III

  

IV

V

VI VII

  A1M - - 148 - 146 - - - 147 A1B 128 130 - 132 - - 130 - 130 120 mg/L A2A - - - - - - - - -

  1

  Total 13 100%%

  1 7,7% A1A 0% A2A 0% A3A 0%

  1

  1 7,7% A3M

  1

  5 38,5% A1M 0% A2M

  1

  1

  1

  1

  1

  4 30,8% A3B

  1

  1

  A2M - - - - - - 138 138 138 A2B 127 118 121 - - 123 125 121 122.5 130 mg/L A3A - - - - - - - - -

  1

  VIII 110 mg/L A1A - - - - - - - - -

  1

  1

  8 A1B

  7

  6

  

5

  4

  3

  2

  1

  A3M - - - - 115 119 - - 117 A3B 105 107 110 - 110 - 105 - 107.4 Lampiran 7. Data Persentase Konsentrasi 2,4-D dengan Posisi Eksplan Terhadap Induksi Kalus Embriogenik Perlakuan ulangan Total Persentase

  2 15,4% A2B

  

Lampiran 8. Master Tabel SPSS saat terbentuknya Kalus Primer

Persentase terbentuknya kalus primer

Faktor A (Tingkat Konsentrasi) Faktor B (Posisi Eksplan) Ulangan Terbentuknya Kalus Primer

  8 110mg/l Basal

  5 120mg/l Apikal

  1 120mg/l Apikal

  4

  3 120mg/l Apikal

  2 120mg/l Apikal

  1 120mg/l Apikal

  8 120mg/l Apikal

  7 110mg/l Basal

  6 110mg/l Basal

  5 110mg/l Basal

  4 110mg/l Basal

  3 110mg/l Basal

  2 110mg/l Basal

  1 110mg/l Basal

  7 110mg/l Median

  110mg/l Apikal

  6 110mg/l Median

  5 110mg/l Median

  4 110mg/l Median

  3 110mg/l Median

  2 110mg/l Median

  1 110mg/l Median

  8 110mg/l Median

  7 110mg/l Apikal

  6 110mg/l Apikal

  5 110mg/l Apikal

  4 110mg/l Apikal

  3 110mg/l Apikal

  2 110mg/l Apikal

  1 110mg/l Apikal

  6

  120mg/l Median

  2

  3

  1 130mg/l Median

  4 130mg/l Median

  5 130mg/l Median

  6

  1 130mg/l Median

  7 130mg/l Median

  8 130mg/l Basal

  1

  1 130mg/l Basal

  1 130mg/l Basal

  2

  3

  1 130mg/l Basal

  4

  1 130mg/l Basal

  5

  1 130mg/l Basal

  6 130mg/l Basal

  7

  1 130mg/l Basal

  8

  1 130mg/l Median

  1 130mg/l Median

  5 120mg/l Median

  1 120mg/l Basal

  6 120mg/l Median

  7 120mg/l Median

  8 120mg/l Basal

  1 120mg/l Basal

  2 120mg/l Basal

  3 120mg/l Basal

  4

  1 120mg/l Basal

  5 120mg/l Basal

  6

  7

  8 130mg/l Median

  1 120mg/l Basal

  8

  1 130mg/l Apikal

  1 130mg/l Apikal

  2 130mg/l Apikal

  3 130mg/l Apikal

  4 130mg/l Apikal

  5 130mg/l Apikal

  6 130mg/l Apikal

  7 130mg/l Apikal

  1

  

Lampiran 9. Analisis varian hubungan konsentrasi terhadap posisi eksplan

Value Label N Konsentrasi 2.4-D

  24 120mg/l Basal .2500 .46291

  24 Total .2361 .42767

  24 Apikal .4583 .50898

  24 Median .1667 .38069

  24 Total Basal .0833 .28233

  8 Total .4167 .50361

  8 Apikal .8750 .35355

  8 Median .3750 .51755

  24 130mg/l Basal .0000 .00000

  8 Total .2917 .46431

  8 Apikal .5000 .53452

  8 Median .1250 .35355

  8 Total .0000 .00000

  1.00 110mg/l

  8 Apikal .0000 .00000

  8 Median .0000 .00000

  Eksplan Mean Std. Deviation N 110mg/l Basal .0000 .00000

  2,4-D Posisi

  24 Konsentrasi.

  3.00 Apikal

  24

  2.00 Median

  24

  1.00 Basal

  24 Posisi Eksplan

  24 2.00 120mg/l 24 3.00 130mg/l

  72 Lampiran 10. Uji homogenitas posisi eksplan dengan konsentrasi 2,4-D F df1 df2 Sig.

  Lampiran 11. Uji lanjut Post Hoc induksi kalus primer

1. Konsentrasi 2.4-D

  Dependent Variable:Kalus Primer 95% Confidence Interval Konsentras i 2.4-D Mean Std. Error Lower Bound Upper Bound 110mg/l -3.701E-17 .069 -.137 .137 120mg/l .292 .069 .154 .429 130mg/l .417 .069 .279 .554

2. Posisi Eksplan

  Dependent Variable:Kalus Primer 95% Confidence Interval Posisi Eksplan Mean Std. Error Lower Bound Upper Bound Basal .083 .069 -.054 .221 Median .167 .069 .029 .304 Apikal .458 .069 .321 .596

3. Konsentrasi 2,4-D * Posisi Eksplan

  Dependent Variable:Kalus Primer 95% Confidence Interval Konsentrasi Posisi 2.4-D Eksplan Mean Std. Error Lower Bound Upper Bound 110mg/l Basal 2.220E-16 .119 -.238 .238 Median 1.110E-16 .119 -.238 .238 Apikal -4.441E-16 .119 -.238 .238 120mg/l Basal .250 .119 .012 .488 Median .125 .119 -.113 .363 Apikal .500 .119 .262 .738 130mg/l Basal -6.661E-16 .119 -.238 .238 Median .375 .119 .137 .613

  

Apikal .875 .119 .637 1.113

Lampiran 12. Uji lanjut (Post Hoc) Duncan konsentrasi 2,4-D terhadap Induksi Kalus Primer

  130mg/l 24 .4167b Sig. 1.000 .203

Lampiran 13. Uji lanjut (Post Hoc) Duncan Posisi Eksplan terhadap induksi

Kalus primer.

  6 110mg/l Basal

  6

  5 120mg/l Basal

  1 120mg/l Basal

  4

  3 120mg/l Basal

  2 120mg/l Basal

  1 120mg/l Basal

  8 120mg/l Basal

  7 110mg/l Basal

  5 110mg/l Basal

  Duncan a,,b

  4 110mg/l Basal

  3 110mg/l Basal

  2 110mg/l Basal

  1 110mg/l Basal

  110mg/l Basal

  Embriogenik

  (Tingkat Konsentrasi) Faktor B (Posisi Eksplan)

Ulangan

Terbentuknya Kalus

  2 Basal 24 .0833 Median 24 .1667 Apikal 24 .4583 Sig. .394 1.000 Lampiran 14. Master tabel SPSS saat terbentuknya kalus Embriogenik

Persentase terbentuknya kalus embriogenik

Faktor A

  1

  Posisi Eksplan N Subset

  1

  130mg/l Basal

  6 130mg/l Median

  8 130mg/l Median

  1 130mg/l Median

  2

  1 130mg/l Median

  3 130mg/l Median

  4 130mg/l Median

  5 130mg/l Median

  7 130mg/l Median

  7

  8 110mg/l Apikal

  1 110mg/l Apikal

  2 110mg/l Apikal

  3 110mg/l Apikal

  4 110mg/l Apikal

  5 110mg/l Apikal

  6 110mg/l Apikal

  7 110mg/l Apikal

  1 120mg/l Median

  6 120mg/l Median

  5

  3 110mg/l Median

  1 130mg/l Basal

  6 130mg/l Basal

  7

  1 130mg/l Basal

  8

  1 110mg/l Median

  1 110mg/l Median

  2 110mg/l Median

  4 110mg/l Median

  5 120mg/l Median

  5 110mg/l Median

  6 110mg/l Median

  7 110mg/l Median

  8 120mg/l Median

  1 120mg/l Median

  2 120mg/l Median

  3 120mg/l Median

  4 120mg/l Median

  8

  120mg/l Apikal

  7 120mg/l Apikal

  8 130mg/l Apikal

  1 130mg/l Apikal

  2 130mg/l Apikal

  3 130mg/l Apikal

  4 130mg/l Apikal

  5 130mg/l Apikal

  6 130mg/l Apikal

  7 130mg/l Apikal

  8 Sum of Squares df Mean Square F Sig.

  Between Groups 3.083 2 1.542 11.261 .000 Within Groups 2.875 21 .137 Total 5.958

  23 Lampiran 16. Uji lanjut (Post Hoc) Duncan Kalus Embriogenik Duncan a

  Konsentrasi 2.4-D N Subset for alpha = 0.05

  1

  2 110mg/l 8 .0000 120mg/l 8 .5000 130mg/l 8 .8750 Sig. 1.000 .056

  120 mg/L A2B 0.04 0.05 0.06 0.06 0.05 0.07 0.05 0.07 0.441 0.055 130 mg/L A3B 0.11 0.14 0.18 0.15 0.12 0.15 0.17 0.18 1.186 0.148 Lampiran 18. Uji sidik ragam berat basah kalus

Sum of Squares df Mean Square F Sig.

  Between Groups .055 2 .027 96.452 .000 Within Groups .006 21 .000

  Total .061

23 Konsentrasi

  2.4-D N

Subset for alpha = 0.05

  1

  2 110mg/l 8 .04025 a 120mg/l 8 .05513 a 130mg/l 8 .14825 b Sig. .092 1.000

  Lampiran 19. Data Berat Kering Kalus Perlakuan Ulangan

  I II

  III

  IV V

VI VII

  VIII Total rata- rata 110 mg/L A1B 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.058 0.007 120 mg/L A2B 0.01 0,011 0.01 0.01 0.01 0.01 0.01 0.01 0.068 0.009 130 mg/L A3B 0.01 0.02 0.02 0.01 0.01 0.01 0.02 0.02 0.116 0.014

  Lampiran 20. Uji sidik ragam berat kering kalus Sum of Squares df Mean Square F Sig.

  Between Groups .000 2 .000 29.618 .000

  Lampiran 21. Uji lanjut (Post Hoc) Duncan berat kering kalus Subset for alpha = 0.05 Konsentrasi

  2.4-D N

  1

  2 110mg/l 8 .00725 a 120mg/l 8 .00775 a 130mg/l 8 .01450 b Sig. .640 1.000