ART Reskhi O, Endang P, Maria MH Induksi Kalus Embriogenik Abstract

Induksi Kalus Embriogenik Pada Perbanyakan Leek (Reskhi Oktaviani, Endang Pudjihartati, Maria Marina Herawati)

INDUKSI KALUS EMBRIOGENIK PADA PERBANYAKAN LEEK (Allium porrum L.)
EMBRYOGENIC CALLUS INDUCTION ON THE MULTIPLICATION OF LEEK
(Allium porrum L.)
Reskhi Oktaviani1, Endang Pudjihartati2, Maria Marina Herawati2
Diterima 13 April 2012, disetujui 31 Juli 2012

ABSTRACT
Multiplication of leek (Allium porrum L.) by tissue culture is an alternative to the provision of
seedlings, especially if the varieties no tillering and not able to flowering during plant production
in Indonesia. A study was conducted to determine the best various of explant and growth regulator
composition to induce embryogenic callus. Primary somatic embryos obtained by planted explant
leaf base and root tip on MS medium containing 2.4 D and BA. At a later stage, primary somatic
embryos will form a secondary somatic embryo after subculture. 1 month old root tips are the best
explants for induce embryogenic callus, while ZPT 1.0 mg / l 2.4 D + 1.0 mg / l BA was the most
appropriate composition to induce embryogenic callus.
Keywords: Allium porrum, multiplication, explant, 2,4-D, BA, callus induction

1

Alumni Fakultas Pertanian & Bisnis Universitas Kristen Satya Wacana, E-mail: reskhioktaviani@yahoo.com
Dosen Fakultas Pertanian & Bisnis Universitas Kristen Satya Wacana, E-mail: endangeps@staff.uksw.edu
Jl. Diponegoro 52-60 Salatiga
3
Pengelola pembibitan Leek di Purwokerto
2

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