Microbial Assay of Cyprofloxacin in a Bone Implant Chitosan-Bovine Hydroxyapatite with Cross-Linker Glutaraldehyde towards Staphilococcus aureus ATCC25923 Repository - UNAIR REPOSITORY
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ISBN: 978-602-60569-3-1
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PROCEEDING
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st lnternational Conference
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Faculty of Pharmacy I Faculty of Dentistry |
Faculty of Medicine I
Faculty of Public Health I School of Nursing
University of Jember, lndonesia
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EDITORS
Ari Satia Nugraha, SF., GDipSc., Msc-res, Ph.D., Apt.
Lusia Oktora RKS, S.F., M.Sc., Apt.
lka Puspita Dewi, S.Farm., M.Biomed., Apt.
Afifah Machclaurin, S.Farm., M.Sc., Apt.
Antonius Nugraha Widhi. Pratama, S.Farm., MPH., Apt.
11
CONFERENCE COMMITTEE
Steering Committee
Event Division
Drs. Moh. Hasan., Ph.D (Rector of
Diana Holidah, S.F., M.Farm., Apt.
DR. drg. I Dewa Ayu Susilowati, M.Kes.
dr. Hairrudin, M.Kes.
DR. Farida Wahyuningtyas, SKM., M.Kes.
Ns. Wantiya, S.Kep., M.Kep.
dr. Ancah Caesarina Novi M., Ph.D.
University of Jember)
Drs. Zulfikar, Ph.D (Vice Rector for
Academic Affairs University of Jember)
Prof Drs Bambang Kuswandi, M.Sc., PhD
drg. Rahardyan Parnaaji, M.Kes., Sp.Pros.
dr. Enny Suswati, M.Kes.
lrma Prasetyowati, SKM., M.Kes.
Ns. Lantin Su listyorini, S.Kep.,M.Kep.
Scientific Division (Editors)
Ari Satia Nugraha, SF., GDipSc., MSc-res,
Ph.D., Apt.
Lusia Oktora RKS, S.F., M.Sc., Apt.
lka Puspita Dewi, S.Farm., M.Biomed., Apt.
Afifah Machclaurin, S.Farm., M.Sc., Apt.
Antonius Nugraha Widhi. Pratama,
S.Farm., MPH., Apt.
Dr. drg. Masniari Novita, M.Kes.
dr. Rini Riyanti, Sp.PK.
Yunus Ariyanto, S.KM., M.Kes
Ns. Achmad Rifa'i, M.S.
Organizing Committee
Chairmain
Lestyo Wulandari, S.Si., M.Farm., Apt.
Secretary
Endah Puspitasari, S.Farm., M.Sc., Apt.
Treasurer
Yuni Retnaningtyas, S.Si., M.Si., Apt.
Nia Kristiningrum, M.Farm., Apt.
Secretariat, Publication, and
SponsorshipDivision
Logistic Division
Eka Deddy lrawan, S.Si., M.Sc., Apt.
Dwi Nurahmanto, S.Farm., M.Sc., Apt.
dr. Cicih Komariah Sp.M.
Anita Dewi Moelyaningrum, S.KM., M.Kes.
drg. Ayu Mas Hartini, Sp.PM.
Ns. Emi Wuri W., M.Kep., Sp.KepJ.
111
CONTENT
PREFACE
..,.........t
EDrTORS........
.
CONFERENCE COMMITTEE
iii
coNTENT..........
iv
PHARMACY.............
................... 1
COMMUNITY PHARMACISTS' COUNSELLING SKILLS
M
EDTCATTONS
ON
OVER.THE-COUNTER (OTC)
....................2
FORMULATION AND OPTIMIZATION OF CAFFEINE NANOEMULSION USING FACTORIAL
DESTGN STUDY..
.................. 6
EFFECI
OF
7% : 2%
COMBINATION SODIUM ALGINATE-GELATN
CONTENT tN
CHARACIERISTIC AND ANTI M ICROBIAL ACTIVITY OF PROBIOTIC MICROSPHERES Lactobacillus
acidophilus.......
................ 10
ANTIDIABETIC ACTIVIW OF POWDER AND ETHANOLTC EXTRACT OF ANTLTON (Myrmeleon
sp.) ON WISTAR STRAIN WHITE MALE RATS WITH GLUCOSE PRELoAD
74
ANTIBACTERIAL AND ANTIBIOFILM POTENTIAL OF ETHANOLIC EXTRACT FROM BINTARO
FLOWER (Cerbera odollam) AGAINST Staphylococcus aureus ATCC 6538.............................. 17
STRUCTURE MODtFtCAT|ON
AND
MOLECULAR MODELTNG
OF
1_(BENZOYLOXY)UREA
DERIVATIVES AS ANTICANCER DRUG CANDIDATES..
................ 20
CHARACTERIZATION AND THE RELEASE TEST OF ANTI-AGING TRETINOIN IN NANOEMULSION
USING OLIVE OIL.......
EFFECT OF PARTICLE SIZE
AND SURFACE CHARGE ON THE UPTAKE AND IMMUNE RESPONSE
OF OVALBUMIN.ALGINATE MICROSPHERES
ANTIHYPE RCHOLESTE ROLEM
lC
EFFECT
OF
Arcangelisia
flava STEM
HYPERLIPIDEMIC RATS........
EXTRACT tN
.......'..'.... 31
GREEN TEA EXTRACT EFFECT ON BLOOD GLUCOSE LEVEL AND LIVER HISTOPATHOLOGY IN
D|ABETTC MtCE ...........
............ 35
TH REE-WAVELENGTH SPECTROPHOTOM ETRIC M ETHOD VALI DATION FOR DETERM INATION
OF PREDNISONE TABLET CONTAINING COLORING DYES ....
39
TNFLUENCE OF OLETC ACtD ON THE tN VTTRO PENETRATTON OF DTCLOFENAC SODTUM GEL
[3
ANTIOXIDANT ACTIVITY OF METHANOL EXTRACTS FROM THE STEM BARK OF MANGROVE
PLANT Rhizophora mucronata............
47
PHYTOCHEMICAL AND ANTIOXIDANT ACItVtTy of MANGROVE pLANT Soneratia sp. ........... 51
EFFECT OF SOLID LIPID NANOPARTICLE (SLN) AND NANO STRUCTURE LIPID CARRIER (NLC)
SYSTEM ON ANTIOXIDANT STABILITY OF TOMATO EXTRACT (LtptD: CETyL ALCOHOL AND
lsoPRoPYL MYRTSTATE)......
....................... 55
EFFECTIVENESS
OF BINTARO (Cerberra odollam Gaertn.) LEAF ETHANOLTC EXTRACT
AGAINST Staphylococcus aureus I N-VITRO BtOFILM FORMATION
lY
59
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29 " " """""" "
'toNVHrr rv3-t llllol vlt8vuv ro NollvNl€t^of All^llf,v lNVClxoll-NV lo ACnrs
t45
CORRELATION OF CD4 WITH TOTAL LYMPHOCYTE COUNTS IN HIV PATIENTS
...
DENTISTRY...........
... 148
Determinants of HIV/AIDS Awareness and Knowledge in Tanah Papua, lndonesia
""""""
149
THE ABILITY OF ANTI-INFLAMMATORY JATROPHA CURCAS LEAF EXTRACT AT COX'2
1-54
EXPRESSION ON MONOCYTES WERE EXPOSED LPS
........................ 158
NOVEL METHOD THYROID HORMONE MEASUREMENT ...."....
ROBUSTA COFFEE BEANS INCREASE LEVELS OF TNF-
o
AS
A
RESPONSE
mutans
TO Streptococcus
762
THE LEVE6 OF TNF-A lN GINGIVAL CREVICULAR FLUID (GCF) OF OSING TRIBE WOMEN WITH
................ 165
OCCLUSAL DISHARMONY...,...,......
Robusta Coffee Bean Extract (Coffea robusta) on the Viability
Exposed by Porphyromonas gingivalis.........'
Effects
of
of Neutrophils
...... 169
ROBUSTA COFFEE BEANS DECREASE OF INFLAMMATION IN DENTAL CARIES""......".......'' 173
................ L77
The Progressive Low Chronic lnflammation on OralTissues ln Elderly
DENTAL CARIES
IN
PREGNANT WOMEN WHO VISITED POSYANDU OF SEVERAL PUBLIC
782
HEALTH CENTERS IN JEMBER
Role of Che moattractant Chemokine (SDF-1/CXCR4) ln Bone Marrow
Niche "
" " " " " ' 185
Establishment of a Rat Model of Temporomandibular Joint Osteoarthritis using lntraarticular
..... 190
lnjection of Complete Freund's Adjuvant.......
.....794
PUBLIC HEALTH
RECIPROCAL DETERMINISM "DAKOCAN" CHALLENGE EFFORTS
CASES IN JEMBER DISTRICT.......
TO REDUCE HIV AND AIDS
. 195
IRON TABLETS DISTRIBUTION OF PREGNANT WOMAN IN THE DISTRICT AND CITY OF EAST
200
JAVA PROVINCE.....
RISK MANAGEMENT OF DUE TO EXPOSURE TO PESTICIDE POISONING FOR TOBACCO
............204
FARMERS IN THE JEMBER DISTRICT..
AN
OVERVIEW
OF MOTHER KNOWLEDGE AFTER GIVING BIRTH ABOUT
........ 208
BREASTFEEDING ......
D|SASTER PREPAREDNESS
EXCLUSIVE
AT PUBLIC HEALTH CENTER (PHC) BY SCORING ANALYSIS OF
GENERAL ASPECTS, HEALTH CARE, SURVEILLANCE, ENVIRONMENTAL SANITATION AND
...........272
LOGISTICS
INDEPENDENT FAMILY PLANNING IN RURALAND URBAN AREAS GRESIK DISTRICT .....,."...71.5
UNMET NEED FOR FAMILY PLANNING ON ELIGIBLE COUPLE IN INDONESIA: 2OO7 IDHS DATA
........219
ANALYSIS
shells That Have been Polluted by lead around Youtefa Bay in Jayapura City That Have
........................ 223
Potential Risk Of Non Carcinogenik.'...'. ........
IMPLEMENTATION DIARRHEAL SURVEILANCE REPORT INFORMATION SYSTEM
227
WITH WATERFALL METHOD IN HEALTH DEVELOPMENT OF JEMBER......"...
DESIGN
AND
\t
LOCAL WISDOM OF JEMBER COMMUNITY
IN REDUCING CYANOGENIC
LEVELS
URINE THIOCYANATE LEVELS
TO LOWER
229
UNDERWEIGHT AND MORBIDITY STATUS AMONG UNDER FIVE YEARS CHILDREN IN
) ?4.
suRABAYA..............
CONDOM USE AMONG EXIT CLIENTS OF FEMALE SEXUAL WORKERS FOR PREVENTION
237
HIV/AIDS IN MAKASSAR
THE SOCIAL SUPPORT AND PREVALENCE EMESIS GRAVIDARIUM ON PREGNANT MOTHER IN
TRIMESTER I AT PUSKESMAS KEMBARAN I BANYUMAS REGENCY...............,..,..,..,..,............247
245
NURSING..
We need a bigger bomb: a community attempt on fighting dengue fever in a suburban
Su ra
,.,.....,.......'.,'.,..,246
baya, lndonesia
APPLICATION OF STANDART NURSING LANGUAGE (NANDA, NOC. NIC) USING SOCIAL MEDIA:
INSTAGRAM@
TO
INCREASE INFORMATION SEEKING BEHAVIOUR
AND MOTIVATION
OF
.......250
NURSING STUDENT.....
THE EFFECT OF ONION (Allium ascalonicum L.) COMPRES TOWARD BODY TEMPERATURE OF
CHILDREN WITH HIPERTERMIA IN BOUGENVILLE ROOM DR. HARYOTO LUMAJANG HOSPITAL
.......253
ACHIEVEMENT OF BLOOD PRESSURE TARGET WITH MEDICATION ADHERENCE AND SODIUM
CONSUM PTION IN SAIFUL ANWAR GENERAL HOSPITAL OUTPATIENT CLIN IC... ....,.,,..,........251
EFFECT
OF INSTRUCTIONAL VIDEO OF SPLINTING PROCEDURE TO NURSING
spLtNTtNG SKTLL (PREHOSPTTAL
SETTING)....
STUDENTS
......................261
THE CORRELATION BETWEEN NURSE PERFORMANCE & THE LEVEL OF JAMKESMAS PATIENT
........266
SATISFACTION IN DAHLIA II WARD, NGUDI WALUYO WLINGI HOSPITAL
How To Maintain High Quality Cardiopulmonary Resuscitation ln Adults: Literature Review
270
SMOKING BEHAVIOUR AMONG MIDDLE AND LATE ADOLESCENTS IN A SUB DISTRICI OF
275
MALANG DISTRICT, EAST JAVA, IN DONESI4............
THE DIFFERENCES DECLINE BREAST ENGORGEMENT CONDUCTED CONVENTIONAL METHODS
(BREAST MASSAGE) WITH HERB YEAST-KATU..
v11
282
MICROBIAL ASSAY OF CYPROFLOXACIN IN A BONE IMPLANT
(cHrTosAN -BOVTNE HYDROXYAPATTTE WrrH CROSS-LINKER
G
LUTARALD E HYD E ) TOWAR DS Sto p h i I ococcu s o u re u s
ATCC25923
Esti Hendradi, Dewi Melani Hariyadi, Muhammad Faris Adrianto
Faculty of Pharmacy, Universitas Airlangga, Jl. Dharmawangsa Dalam Surabaya 60286.
E-mail ad d ress: estihend rad i@ya hoo.com
INTRODUCTION
Bone is one part of the body has an important role
to support the body's physiological functions (Porter
et al., 2009). Complications of bone diseases and
bone disorders caused by traumatic accidents may
result in a gap (defect) on the bone. The healing
process of damage or fracture is determined by the
level of trauma and soft tissue damage (strobel et
al., 2011). Some cases of damage or injury to the
bone can not undergo natural recovery (Porter et al.,
2009). Therefore, clinical rehabilitation to overcome
the defect on the bone is expected to increase in line
with population growth (Mourino et al., 2010).
Treatment rehabilitation of bone cannot be
separated from the risk of infection complications.
Complications of bacterial infections can be treated
with antibiotics. However, in the case of a crack
(defect) occurs devascularity of bone tissue so that
the delivery of antibiotics to the target tissue to be
blocked. This resulted in the concentration of the
antibiotic to the target so low that it cannot
penetrate the bacteria. The condition can lead to
bacterial resistance to antibiotics (Li et al., 2010). A
high dose of antibiotics in the long term experienced
problems because it can cause systemic toxicity and
side effects (Mourino et a1.,2010). To overcome
these problems, antibiotics can be done locally using
a certain drug delivery systems. The purpose of such
delivery systems is to provide drug concentration in
a
specific location and ensure the drug release
profile for a certain time period (Dubnika et al.,
2072). Dtug delivery locally has several advantages,
among others, (a) the systemic effects can be
avoided, (b) the amount of drugs used less and
secure, and (c) the efficacy and efficiency of drug
delivery locally can be achieved (Harmankaya et al.,
2013). Administration of antibiotics locally also to
minimize side effects and risk of toxicity compared
to administration of systemic antibiotics. ln addition,
antibiotics locally also allows conduction in target
tissues with high concentration (Mourino et al.,
2010). The release of antibiotics on the target
network is expected to last continuously for a certain
time and achieve a greater concentration than the
minimum inhibitory concentration {MlC). Drug
delivery systems in a controlled manner (controlled
release system) can help increase the bioavailability
of
antibiotics
in target tissues. The
system
is
designed to release the drug at the expected
location at a rate appropriate for a certain time
period (Mourino et a1.,2010). ln a previous study
showed that a good composite is Ciprofloxacin: BHA:
chitosan = 10:30:60. Cross linker with
glutaraldehyde \cAl o.7% and with 10% active
ingredient Ciprofloxacin can release Cyprofloxacin
for 30 days (Hendradi et al, 2015). This research will
be seen potency against Staphilococcus aureus
ATCC25923 Ciprofloxacin for 30 days.
MATERIAI. AND METHODS
Materials
Ciprofloxacin (Shangyu Jingxin Pharmaceutical Co.
Ltd) ; Bovine Hydroxyapatite (BHA) diperoleh dari
Bank Jaringan RSUD DR Soetomo Surabaya; Chitosan
(Biotech lndonesia); glutaraldehid 25% p.a (Merck
Milipore-German); asam asetat glacial p.a (Merck),
Na2HPO4
p.a (Merck), KzHPOT p.a, KH2POa p.a, Nacl
p.a (Merck-German) and Aquabidest
Methods
T.Fomulotion of Bovine Hydrcryopotite - chitosqn-
ciptofloxocii implont
The composition of formulations
of implant before
adding glutaraldehyde was mentioned in Table 1.
The implant produced by compression method.
Ciprofloxacin were dissolved in aquabidest, Bovine
Hydroxyapatite added gradually and mixed until
homogen with ciprofloxacin. chitosan powder were
added to ciprofloxacin-Bovine Hydroxyapatite blend
and mixed until homogen. Aquabidest were added
gradually
with continous stirring until form wet
granules mass. Wet granules mass were sieved using
1 mm siever and dried overnight (24 hours) at 40 'C
to obtain dried granules. Dried granules were
in
glutaraldehyde solution (O.7yo
concentration) for 24 hours until the colour was
immersed
change. Granules were washed with aquabidestilata
to remove the residual glutaraldehyde. At the final
stage, granules were washed
100
uith phosphate buffer saline (PBS) pH 7.40. Granules
r€re dried in oven at 40'C for 24 hours. Dried
press machine with 4.0 mm diameter and the
s)mpression pressure was 2 tons (Hendradi et al,
4. Test potency dilution method ol ontibiotic
Test antibiotic poteny dilution method prints holes
(wells) design 3-3. A total of 10 ml of inoculum of
Staphylococcus aureus ATCC 25923 was inserted into
201s).
Table 1. The composition of implant formulation
Nutrient Agar that had thawed and then allowed to
granules were weighed 100 mg, pressed using tablet
Compound
Concentration
l%l
Cyprofloxacin
10
BHA
30
Chitosan
60
the tube containing the seed layer 8 ml
stand up to a temperature of 45 Homogenized with a vortex, then poured
over the surface of the base layer has been solidified
in a petri dish, allowed to solidifv. Hole was made in
order to use the printer for sterile. Each hole was
2
placed in a vial containing 5 ml of phosphate buffer
(PBS) pH 7.4. Vial was placed in a shelf and
inhibition zone diameter compared with the border
of the effective inhibition zone, the minimum range
sline
ircubated in waterbath at 37'C
t
0.5 "C. Sampling
U:as conducted by pippeting 1 ml of elution fluids at
Fedetermined time intervals (1, 2,3, until ....30 )
day and replaced with fresh buffer to maintain sink
condition. Appropriate dilution was prepared using
,Sosphate buffer saline (PBS) pH 7.a. The release of
c!)rofloxacin HCL from the implants was determined
ir triplicate using microbial assay.
I
hole was measured by using a caliper. The resulting
of 14-16 mm (Depkes R|,2014)
RESULT AND DISCUSSION
7.
lmplont ol cyptofloxocin
The implant formulation showed in figure 2.
Optimizotion oI the minimum inhihitory concentrdtion
ra
}tc)
ri
n
:k
J,
CI
50eC.
evenly
filled with the test solution and standard solution as
50,0U1 for each hole and then incubated at 37eC for
24 hours. Diameter of inhibition zone formed at each
Releosed Study ol Cyptofloxocin
The release study of cyprofloxacin from implant was
done as sample for test potential. lmplant was
f,.
media
llade six standard solution with a concentration of
cyprofloxacin 0.06 ug/ml - 2 $glml. Cyprofloxacin
qandard solution that has been created is inserted
irto the hole that has formed on nutrient agar,
$ich had previously been inoculated with
9aphylococcus aureus ATCC 25923. lncubation for
ao
,
Figure 2, lmplantof cyprofloxacin
24 hours at a temperature of 37"C. Observe
d|e inhibition zone is formed. Lowest levels of
-*ribitory zone where there is a minimum inhibitory
@ncentration of cyprofloxacin.
/\ d\ /\/\
ciprofloxacin towads Stophylococcus dureus AfCC
25923 was2.0 tE/ml showed in table 2 and Figure 3.
ln figure 3 showed that the MIC of cyprofloxacin was
n
nnnn n t
n---nn
;;;;1,
.r-.1,1,"t-[
1
0
The Minimdl lnhibitory Concentrction (MIC) oI
The Minimal lnhibitory Concentration (MlC) of
/\
Pippd.d I500 LL.d lES l!00
'l
2.
cyprofloxdcin
2.0 [c/ml
(U1)
u!
f
o
o
o
o
o
i0 0 +inbdor puti!
\
[d.
1
MIC Determination of yiprofloxacin towards
Lphylococcus aureus ATCC 25923
Eure 1.
Figure 3.The Minimal lnhibitory Concentration (MlC) of
cyprofl oxacin towa.ds
ATCC 25923
101
towatds Stophylococcus
ou reus
Table 2. The Minimal tnhibitory Concentration
(MlC) of Cyprofloxacin towards Stophylococcus
in Figure 5. lt's mean that the
implant formulation of cyprofloxacin had the ability
to against Staphylococcus aureus ATCC25923.
ATCC25923 showed
dureus ATCC 25921
lt5 ------------------------
:R
Replication
Concent
ration
Zona
(pclml)
1
Diamet
lnhibiti
er
on
Zona
of
Zoaa
er
on
Zona
14.00
mm
of
n5
x
=
105
U
9!
+PoLEy
--.Lr.l
ol
Cirond.cln
Pot nc,
0.7
Ol
olclFoioEh
lnhibiti
on
+
Diamet
tnhibiti
lnhibiti
2.O
.E
Replication 2
on
+
14.30
mm
15
12
ta la tt
2a 12 2a
u r t
Day
Figure
1.0
l l 5 7 ta
5.
Potency cyprofloxacin profil vs time (day
released from implant
BHA-chitosan-ciprofloxacin
glutaraldehyde) in phosphate buffer salinc
Towards Staphylococcus aureus ATCC25923 tor :I)
days. Each value represents the mean a S.D. of 3
0.7%
0.5
0.25
determinations
o.72
0.06
coNcruSroN
The result showed that the potency of c.yprofloxacin
Releqsed Study of Cyprofloxocin
The data
of
released study was found from the
previous study (Hendradi, et.a1.,2015). ln this data
the concentration of Cyprofloxacin
every day was in therapeutics level (2-50
showed that
release
pg/ml). lt's showed in figure 4
in the formula could meet the requirements for
antibiotic microbial assay that was 80-125% to
inhibit the bactetia Stophylococcus aureus AICC
25923 for 30 days.
Acknowledgement
The authors acknowledge the financial support
received from DlKTl, for their support and
encouragement in PUPT program
@ .00
REFERENCES
1.
p.00
!o &
f hsBpEJlit Cllc J0 srirro
d) .00
0.00 5.00 10.00 15.00 20.00 25.00 30.00
2.
I)rv
Figure 4. Concentration of cyprofloxacin profil vs time
(day) released from implant BHA-chitosan-ciprofloxacin
glutaraldehyde in phosphate buffer saline Towards
Staphylococcus aureus ATCC25923 for 30 days, Each
value represents the mean + S.D. of 3 determinations
(Hendradi et al, 2015)
0.7%
3.
Poten.y of Cyptofloxqcin released
to
3.
Harmankaya, N., Karlsson, J., Palmquist, A.,
Halvarsson, M., lgawa, K., Andersson, M., and
Tengvall, P. , 2013. Raloxifene and alendronate
containing thin mesoporous titanium oxide films
improve implant fixation to bone. Acta
Biomote riol la, V ol. 9, p. 7 064-7073.
Hendradi E. Hariyadi, D.E., Adrianto,M.F, The
effect of different cross link
agent
of ciprofloxacin implant. Reseorch
Stophilococcus
that the potency of cyprofloxacin
against Staphylococcus aureus ATCC25923 could
meet the requirements for antibiotic microbial assay
that was 80-125%. The results was similar with the
concentration of cyprofloxacin released from
implant (Hendradi, et al., 2015). The results of
cyprofloxacin towards Staphylococcus aureus
193-199
glutaraldehyde and genipin in composites to the
physicochemical characteristics and the release
duteus ATCC 25923
The result showed
Dubnika, A., Loca, D., and Cimdina, L.8.,2072.
Functionalized hydroxyapatite scaffolds coated
with sodium alginate and chitosan for controlled
drug delivery. Polymer Science, Vol. 6t (3), p.
4.
in
bmitted 2016.
Li, 8., Brown, K.V., Wenke, J.C., and Guelcher,
S.A., 2010. Sustained release of vancomycin
from polyurethane scaffolds inhibits infection of
bone wounds in a rat femoral segmental defect
model. Journol of Controlled Releose, Vol. 745,
p.227-230.
Pho rmoce utico I Scie n ce. 5u
5. Mourino V,
102
Boccaccini
AR. Bone
tissue
engineering therapeutics
le
ly
6.
:
controlled drug
Strobel C, Bormann N, Romacker A,
G, Wildemann B. Sequential
7
delivery in three-dimensional scaffolds. J R Soc
Schmidmaier,
lnterfoce 2010;7 :209-27 .
Porter JR, Ruckh TT, Popat KC, Bone tissue
engineering:A review in bone biomimetics and
or three (gentamicin,
drug delivery strategies. Biotechnol. Prog
coating
2009;25;1539-1553.
2OLL;!56t37 -45.
release kinetics of two (gentamicin and BMP-2)
substances from
ay
in
1e
30
3
tn
or
to
:c
103
on
IGF-1 and BMP-2)
a one component
implants.
J
polymeric
Controlled
Releose
1
ISBN: 978-602-60569-3-1
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{
PROCEEDING
1" ICMHS
st lnternational Conference
1
on lVledicine and Health Sciences
a
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susta !
nal Collaboration to Achieve
le Development Goals (SDGs)
I
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M^';ft'"",:ki:l:
-"Ilndonesia
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Hosted by:
Faculty of Pharmacy I Faculty of Dentistry |
Faculty of Medicine I
Faculty of Public Health I School of Nursing
University of Jember, lndonesia
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Insan$arytasi
EDITORS
Ari Satia Nugraha, SF., GDipSc., Msc-res, Ph.D., Apt.
Lusia Oktora RKS, S.F., M.Sc., Apt.
lka Puspita Dewi, S.Farm., M.Biomed., Apt.
Afifah Machclaurin, S.Farm., M.Sc., Apt.
Antonius Nugraha Widhi. Pratama, S.Farm., MPH., Apt.
11
CONFERENCE COMMITTEE
Steering Committee
Event Division
Drs. Moh. Hasan., Ph.D (Rector of
Diana Holidah, S.F., M.Farm., Apt.
DR. drg. I Dewa Ayu Susilowati, M.Kes.
dr. Hairrudin, M.Kes.
DR. Farida Wahyuningtyas, SKM., M.Kes.
Ns. Wantiya, S.Kep., M.Kep.
dr. Ancah Caesarina Novi M., Ph.D.
University of Jember)
Drs. Zulfikar, Ph.D (Vice Rector for
Academic Affairs University of Jember)
Prof Drs Bambang Kuswandi, M.Sc., PhD
drg. Rahardyan Parnaaji, M.Kes., Sp.Pros.
dr. Enny Suswati, M.Kes.
lrma Prasetyowati, SKM., M.Kes.
Ns. Lantin Su listyorini, S.Kep.,M.Kep.
Scientific Division (Editors)
Ari Satia Nugraha, SF., GDipSc., MSc-res,
Ph.D., Apt.
Lusia Oktora RKS, S.F., M.Sc., Apt.
lka Puspita Dewi, S.Farm., M.Biomed., Apt.
Afifah Machclaurin, S.Farm., M.Sc., Apt.
Antonius Nugraha Widhi. Pratama,
S.Farm., MPH., Apt.
Dr. drg. Masniari Novita, M.Kes.
dr. Rini Riyanti, Sp.PK.
Yunus Ariyanto, S.KM., M.Kes
Ns. Achmad Rifa'i, M.S.
Organizing Committee
Chairmain
Lestyo Wulandari, S.Si., M.Farm., Apt.
Secretary
Endah Puspitasari, S.Farm., M.Sc., Apt.
Treasurer
Yuni Retnaningtyas, S.Si., M.Si., Apt.
Nia Kristiningrum, M.Farm., Apt.
Secretariat, Publication, and
SponsorshipDivision
Logistic Division
Eka Deddy lrawan, S.Si., M.Sc., Apt.
Dwi Nurahmanto, S.Farm., M.Sc., Apt.
dr. Cicih Komariah Sp.M.
Anita Dewi Moelyaningrum, S.KM., M.Kes.
drg. Ayu Mas Hartini, Sp.PM.
Ns. Emi Wuri W., M.Kep., Sp.KepJ.
111
CONTENT
PREFACE
..,.........t
EDrTORS........
.
CONFERENCE COMMITTEE
iii
coNTENT..........
iv
PHARMACY.............
................... 1
COMMUNITY PHARMACISTS' COUNSELLING SKILLS
M
EDTCATTONS
ON
OVER.THE-COUNTER (OTC)
....................2
FORMULATION AND OPTIMIZATION OF CAFFEINE NANOEMULSION USING FACTORIAL
DESTGN STUDY..
.................. 6
EFFECI
OF
7% : 2%
COMBINATION SODIUM ALGINATE-GELATN
CONTENT tN
CHARACIERISTIC AND ANTI M ICROBIAL ACTIVITY OF PROBIOTIC MICROSPHERES Lactobacillus
acidophilus.......
................ 10
ANTIDIABETIC ACTIVIW OF POWDER AND ETHANOLTC EXTRACT OF ANTLTON (Myrmeleon
sp.) ON WISTAR STRAIN WHITE MALE RATS WITH GLUCOSE PRELoAD
74
ANTIBACTERIAL AND ANTIBIOFILM POTENTIAL OF ETHANOLIC EXTRACT FROM BINTARO
FLOWER (Cerbera odollam) AGAINST Staphylococcus aureus ATCC 6538.............................. 17
STRUCTURE MODtFtCAT|ON
AND
MOLECULAR MODELTNG
OF
1_(BENZOYLOXY)UREA
DERIVATIVES AS ANTICANCER DRUG CANDIDATES..
................ 20
CHARACTERIZATION AND THE RELEASE TEST OF ANTI-AGING TRETINOIN IN NANOEMULSION
USING OLIVE OIL.......
EFFECT OF PARTICLE SIZE
AND SURFACE CHARGE ON THE UPTAKE AND IMMUNE RESPONSE
OF OVALBUMIN.ALGINATE MICROSPHERES
ANTIHYPE RCHOLESTE ROLEM
lC
EFFECT
OF
Arcangelisia
flava STEM
HYPERLIPIDEMIC RATS........
EXTRACT tN
.......'..'.... 31
GREEN TEA EXTRACT EFFECT ON BLOOD GLUCOSE LEVEL AND LIVER HISTOPATHOLOGY IN
D|ABETTC MtCE ...........
............ 35
TH REE-WAVELENGTH SPECTROPHOTOM ETRIC M ETHOD VALI DATION FOR DETERM INATION
OF PREDNISONE TABLET CONTAINING COLORING DYES ....
39
TNFLUENCE OF OLETC ACtD ON THE tN VTTRO PENETRATTON OF DTCLOFENAC SODTUM GEL
[3
ANTIOXIDANT ACTIVITY OF METHANOL EXTRACTS FROM THE STEM BARK OF MANGROVE
PLANT Rhizophora mucronata............
47
PHYTOCHEMICAL AND ANTIOXIDANT ACItVtTy of MANGROVE pLANT Soneratia sp. ........... 51
EFFECT OF SOLID LIPID NANOPARTICLE (SLN) AND NANO STRUCTURE LIPID CARRIER (NLC)
SYSTEM ON ANTIOXIDANT STABILITY OF TOMATO EXTRACT (LtptD: CETyL ALCOHOL AND
lsoPRoPYL MYRTSTATE)......
....................... 55
EFFECTIVENESS
OF BINTARO (Cerberra odollam Gaertn.) LEAF ETHANOLTC EXTRACT
AGAINST Staphylococcus aureus I N-VITRO BtOFILM FORMATION
lY
59
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" " I1vu1x1 ullvM]vlld ul^ oll l.l'llsou oNV rlvul-xl
29 " " """""" "
'toNVHrr rv3-t llllol vlt8vuv ro NollvNl€t^of All^llf,v lNVClxoll-NV lo ACnrs
t45
CORRELATION OF CD4 WITH TOTAL LYMPHOCYTE COUNTS IN HIV PATIENTS
...
DENTISTRY...........
... 148
Determinants of HIV/AIDS Awareness and Knowledge in Tanah Papua, lndonesia
""""""
149
THE ABILITY OF ANTI-INFLAMMATORY JATROPHA CURCAS LEAF EXTRACT AT COX'2
1-54
EXPRESSION ON MONOCYTES WERE EXPOSED LPS
........................ 158
NOVEL METHOD THYROID HORMONE MEASUREMENT ...."....
ROBUSTA COFFEE BEANS INCREASE LEVELS OF TNF-
o
AS
A
RESPONSE
mutans
TO Streptococcus
762
THE LEVE6 OF TNF-A lN GINGIVAL CREVICULAR FLUID (GCF) OF OSING TRIBE WOMEN WITH
................ 165
OCCLUSAL DISHARMONY...,...,......
Robusta Coffee Bean Extract (Coffea robusta) on the Viability
Exposed by Porphyromonas gingivalis.........'
Effects
of
of Neutrophils
...... 169
ROBUSTA COFFEE BEANS DECREASE OF INFLAMMATION IN DENTAL CARIES""......".......'' 173
................ L77
The Progressive Low Chronic lnflammation on OralTissues ln Elderly
DENTAL CARIES
IN
PREGNANT WOMEN WHO VISITED POSYANDU OF SEVERAL PUBLIC
782
HEALTH CENTERS IN JEMBER
Role of Che moattractant Chemokine (SDF-1/CXCR4) ln Bone Marrow
Niche "
" " " " " ' 185
Establishment of a Rat Model of Temporomandibular Joint Osteoarthritis using lntraarticular
..... 190
lnjection of Complete Freund's Adjuvant.......
.....794
PUBLIC HEALTH
RECIPROCAL DETERMINISM "DAKOCAN" CHALLENGE EFFORTS
CASES IN JEMBER DISTRICT.......
TO REDUCE HIV AND AIDS
. 195
IRON TABLETS DISTRIBUTION OF PREGNANT WOMAN IN THE DISTRICT AND CITY OF EAST
200
JAVA PROVINCE.....
RISK MANAGEMENT OF DUE TO EXPOSURE TO PESTICIDE POISONING FOR TOBACCO
............204
FARMERS IN THE JEMBER DISTRICT..
AN
OVERVIEW
OF MOTHER KNOWLEDGE AFTER GIVING BIRTH ABOUT
........ 208
BREASTFEEDING ......
D|SASTER PREPAREDNESS
EXCLUSIVE
AT PUBLIC HEALTH CENTER (PHC) BY SCORING ANALYSIS OF
GENERAL ASPECTS, HEALTH CARE, SURVEILLANCE, ENVIRONMENTAL SANITATION AND
...........272
LOGISTICS
INDEPENDENT FAMILY PLANNING IN RURALAND URBAN AREAS GRESIK DISTRICT .....,."...71.5
UNMET NEED FOR FAMILY PLANNING ON ELIGIBLE COUPLE IN INDONESIA: 2OO7 IDHS DATA
........219
ANALYSIS
shells That Have been Polluted by lead around Youtefa Bay in Jayapura City That Have
........................ 223
Potential Risk Of Non Carcinogenik.'...'. ........
IMPLEMENTATION DIARRHEAL SURVEILANCE REPORT INFORMATION SYSTEM
227
WITH WATERFALL METHOD IN HEALTH DEVELOPMENT OF JEMBER......"...
DESIGN
AND
\t
LOCAL WISDOM OF JEMBER COMMUNITY
IN REDUCING CYANOGENIC
LEVELS
URINE THIOCYANATE LEVELS
TO LOWER
229
UNDERWEIGHT AND MORBIDITY STATUS AMONG UNDER FIVE YEARS CHILDREN IN
) ?4.
suRABAYA..............
CONDOM USE AMONG EXIT CLIENTS OF FEMALE SEXUAL WORKERS FOR PREVENTION
237
HIV/AIDS IN MAKASSAR
THE SOCIAL SUPPORT AND PREVALENCE EMESIS GRAVIDARIUM ON PREGNANT MOTHER IN
TRIMESTER I AT PUSKESMAS KEMBARAN I BANYUMAS REGENCY...............,..,..,..,..,............247
245
NURSING..
We need a bigger bomb: a community attempt on fighting dengue fever in a suburban
Su ra
,.,.....,.......'.,'.,..,246
baya, lndonesia
APPLICATION OF STANDART NURSING LANGUAGE (NANDA, NOC. NIC) USING SOCIAL MEDIA:
INSTAGRAM@
TO
INCREASE INFORMATION SEEKING BEHAVIOUR
AND MOTIVATION
OF
.......250
NURSING STUDENT.....
THE EFFECT OF ONION (Allium ascalonicum L.) COMPRES TOWARD BODY TEMPERATURE OF
CHILDREN WITH HIPERTERMIA IN BOUGENVILLE ROOM DR. HARYOTO LUMAJANG HOSPITAL
.......253
ACHIEVEMENT OF BLOOD PRESSURE TARGET WITH MEDICATION ADHERENCE AND SODIUM
CONSUM PTION IN SAIFUL ANWAR GENERAL HOSPITAL OUTPATIENT CLIN IC... ....,.,,..,........251
EFFECT
OF INSTRUCTIONAL VIDEO OF SPLINTING PROCEDURE TO NURSING
spLtNTtNG SKTLL (PREHOSPTTAL
SETTING)....
STUDENTS
......................261
THE CORRELATION BETWEEN NURSE PERFORMANCE & THE LEVEL OF JAMKESMAS PATIENT
........266
SATISFACTION IN DAHLIA II WARD, NGUDI WALUYO WLINGI HOSPITAL
How To Maintain High Quality Cardiopulmonary Resuscitation ln Adults: Literature Review
270
SMOKING BEHAVIOUR AMONG MIDDLE AND LATE ADOLESCENTS IN A SUB DISTRICI OF
275
MALANG DISTRICT, EAST JAVA, IN DONESI4............
THE DIFFERENCES DECLINE BREAST ENGORGEMENT CONDUCTED CONVENTIONAL METHODS
(BREAST MASSAGE) WITH HERB YEAST-KATU..
v11
282
MICROBIAL ASSAY OF CYPROFLOXACIN IN A BONE IMPLANT
(cHrTosAN -BOVTNE HYDROXYAPATTTE WrrH CROSS-LINKER
G
LUTARALD E HYD E ) TOWAR DS Sto p h i I ococcu s o u re u s
ATCC25923
Esti Hendradi, Dewi Melani Hariyadi, Muhammad Faris Adrianto
Faculty of Pharmacy, Universitas Airlangga, Jl. Dharmawangsa Dalam Surabaya 60286.
E-mail ad d ress: estihend rad i@ya hoo.com
INTRODUCTION
Bone is one part of the body has an important role
to support the body's physiological functions (Porter
et al., 2009). Complications of bone diseases and
bone disorders caused by traumatic accidents may
result in a gap (defect) on the bone. The healing
process of damage or fracture is determined by the
level of trauma and soft tissue damage (strobel et
al., 2011). Some cases of damage or injury to the
bone can not undergo natural recovery (Porter et al.,
2009). Therefore, clinical rehabilitation to overcome
the defect on the bone is expected to increase in line
with population growth (Mourino et al., 2010).
Treatment rehabilitation of bone cannot be
separated from the risk of infection complications.
Complications of bacterial infections can be treated
with antibiotics. However, in the case of a crack
(defect) occurs devascularity of bone tissue so that
the delivery of antibiotics to the target tissue to be
blocked. This resulted in the concentration of the
antibiotic to the target so low that it cannot
penetrate the bacteria. The condition can lead to
bacterial resistance to antibiotics (Li et al., 2010). A
high dose of antibiotics in the long term experienced
problems because it can cause systemic toxicity and
side effects (Mourino et a1.,2010). To overcome
these problems, antibiotics can be done locally using
a certain drug delivery systems. The purpose of such
delivery systems is to provide drug concentration in
a
specific location and ensure the drug release
profile for a certain time period (Dubnika et al.,
2072). Dtug delivery locally has several advantages,
among others, (a) the systemic effects can be
avoided, (b) the amount of drugs used less and
secure, and (c) the efficacy and efficiency of drug
delivery locally can be achieved (Harmankaya et al.,
2013). Administration of antibiotics locally also to
minimize side effects and risk of toxicity compared
to administration of systemic antibiotics. ln addition,
antibiotics locally also allows conduction in target
tissues with high concentration (Mourino et al.,
2010). The release of antibiotics on the target
network is expected to last continuously for a certain
time and achieve a greater concentration than the
minimum inhibitory concentration {MlC). Drug
delivery systems in a controlled manner (controlled
release system) can help increase the bioavailability
of
antibiotics
in target tissues. The
system
is
designed to release the drug at the expected
location at a rate appropriate for a certain time
period (Mourino et a1.,2010). ln a previous study
showed that a good composite is Ciprofloxacin: BHA:
chitosan = 10:30:60. Cross linker with
glutaraldehyde \cAl o.7% and with 10% active
ingredient Ciprofloxacin can release Cyprofloxacin
for 30 days (Hendradi et al, 2015). This research will
be seen potency against Staphilococcus aureus
ATCC25923 Ciprofloxacin for 30 days.
MATERIAI. AND METHODS
Materials
Ciprofloxacin (Shangyu Jingxin Pharmaceutical Co.
Ltd) ; Bovine Hydroxyapatite (BHA) diperoleh dari
Bank Jaringan RSUD DR Soetomo Surabaya; Chitosan
(Biotech lndonesia); glutaraldehid 25% p.a (Merck
Milipore-German); asam asetat glacial p.a (Merck),
Na2HPO4
p.a (Merck), KzHPOT p.a, KH2POa p.a, Nacl
p.a (Merck-German) and Aquabidest
Methods
T.Fomulotion of Bovine Hydrcryopotite - chitosqn-
ciptofloxocii implont
The composition of formulations
of implant before
adding glutaraldehyde was mentioned in Table 1.
The implant produced by compression method.
Ciprofloxacin were dissolved in aquabidest, Bovine
Hydroxyapatite added gradually and mixed until
homogen with ciprofloxacin. chitosan powder were
added to ciprofloxacin-Bovine Hydroxyapatite blend
and mixed until homogen. Aquabidest were added
gradually
with continous stirring until form wet
granules mass. Wet granules mass were sieved using
1 mm siever and dried overnight (24 hours) at 40 'C
to obtain dried granules. Dried granules were
in
glutaraldehyde solution (O.7yo
concentration) for 24 hours until the colour was
immersed
change. Granules were washed with aquabidestilata
to remove the residual glutaraldehyde. At the final
stage, granules were washed
100
uith phosphate buffer saline (PBS) pH 7.40. Granules
r€re dried in oven at 40'C for 24 hours. Dried
press machine with 4.0 mm diameter and the
s)mpression pressure was 2 tons (Hendradi et al,
4. Test potency dilution method ol ontibiotic
Test antibiotic poteny dilution method prints holes
(wells) design 3-3. A total of 10 ml of inoculum of
Staphylococcus aureus ATCC 25923 was inserted into
201s).
Table 1. The composition of implant formulation
Nutrient Agar that had thawed and then allowed to
granules were weighed 100 mg, pressed using tablet
Compound
Concentration
l%l
Cyprofloxacin
10
BHA
30
Chitosan
60
the tube containing the seed layer 8 ml
stand up to a temperature of 45 Homogenized with a vortex, then poured
over the surface of the base layer has been solidified
in a petri dish, allowed to solidifv. Hole was made in
order to use the printer for sterile. Each hole was
2
placed in a vial containing 5 ml of phosphate buffer
(PBS) pH 7.4. Vial was placed in a shelf and
inhibition zone diameter compared with the border
of the effective inhibition zone, the minimum range
sline
ircubated in waterbath at 37'C
t
0.5 "C. Sampling
U:as conducted by pippeting 1 ml of elution fluids at
Fedetermined time intervals (1, 2,3, until ....30 )
day and replaced with fresh buffer to maintain sink
condition. Appropriate dilution was prepared using
,Sosphate buffer saline (PBS) pH 7.a. The release of
c!)rofloxacin HCL from the implants was determined
ir triplicate using microbial assay.
I
hole was measured by using a caliper. The resulting
of 14-16 mm (Depkes R|,2014)
RESULT AND DISCUSSION
7.
lmplont ol cyptofloxocin
The implant formulation showed in figure 2.
Optimizotion oI the minimum inhihitory concentrdtion
ra
}tc)
ri
n
:k
J,
CI
50eC.
evenly
filled with the test solution and standard solution as
50,0U1 for each hole and then incubated at 37eC for
24 hours. Diameter of inhibition zone formed at each
Releosed Study ol Cyptofloxocin
The release study of cyprofloxacin from implant was
done as sample for test potential. lmplant was
f,.
media
llade six standard solution with a concentration of
cyprofloxacin 0.06 ug/ml - 2 $glml. Cyprofloxacin
qandard solution that has been created is inserted
irto the hole that has formed on nutrient agar,
$ich had previously been inoculated with
9aphylococcus aureus ATCC 25923. lncubation for
ao
,
Figure 2, lmplantof cyprofloxacin
24 hours at a temperature of 37"C. Observe
d|e inhibition zone is formed. Lowest levels of
-*ribitory zone where there is a minimum inhibitory
@ncentration of cyprofloxacin.
/\ d\ /\/\
ciprofloxacin towads Stophylococcus dureus AfCC
25923 was2.0 tE/ml showed in table 2 and Figure 3.
ln figure 3 showed that the MIC of cyprofloxacin was
n
nnnn n t
n---nn
;;;;1,
.r-.1,1,"t-[
1
0
The Minimdl lnhibitory Concentrction (MIC) oI
The Minimal lnhibitory Concentration (MlC) of
/\
Pippd.d I500 LL.d lES l!00
'l
2.
cyprofloxdcin
2.0 [c/ml
(U1)
u!
f
o
o
o
o
o
i0 0 +inbdor puti!
\
[d.
1
MIC Determination of yiprofloxacin towards
Lphylococcus aureus ATCC 25923
Eure 1.
Figure 3.The Minimal lnhibitory Concentration (MlC) of
cyprofl oxacin towa.ds
ATCC 25923
101
towatds Stophylococcus
ou reus
Table 2. The Minimal tnhibitory Concentration
(MlC) of Cyprofloxacin towards Stophylococcus
in Figure 5. lt's mean that the
implant formulation of cyprofloxacin had the ability
to against Staphylococcus aureus ATCC25923.
ATCC25923 showed
dureus ATCC 25921
lt5 ------------------------
:R
Replication
Concent
ration
Zona
(pclml)
1
Diamet
lnhibiti
er
on
Zona
of
Zoaa
er
on
Zona
14.00
mm
of
n5
x
=
105
U
9!
+PoLEy
--.Lr.l
ol
Cirond.cln
Pot nc,
0.7
Ol
olclFoioEh
lnhibiti
on
+
Diamet
tnhibiti
lnhibiti
2.O
.E
Replication 2
on
+
14.30
mm
15
12
ta la tt
2a 12 2a
u r t
Day
Figure
1.0
l l 5 7 ta
5.
Potency cyprofloxacin profil vs time (day
released from implant
BHA-chitosan-ciprofloxacin
glutaraldehyde) in phosphate buffer salinc
Towards Staphylococcus aureus ATCC25923 tor :I)
days. Each value represents the mean a S.D. of 3
0.7%
0.5
0.25
determinations
o.72
0.06
coNcruSroN
The result showed that the potency of c.yprofloxacin
Releqsed Study of Cyprofloxocin
The data
of
released study was found from the
previous study (Hendradi, et.a1.,2015). ln this data
the concentration of Cyprofloxacin
every day was in therapeutics level (2-50
showed that
release
pg/ml). lt's showed in figure 4
in the formula could meet the requirements for
antibiotic microbial assay that was 80-125% to
inhibit the bactetia Stophylococcus aureus AICC
25923 for 30 days.
Acknowledgement
The authors acknowledge the financial support
received from DlKTl, for their support and
encouragement in PUPT program
@ .00
REFERENCES
1.
p.00
!o &
f hsBpEJlit Cllc J0 srirro
d) .00
0.00 5.00 10.00 15.00 20.00 25.00 30.00
2.
I)rv
Figure 4. Concentration of cyprofloxacin profil vs time
(day) released from implant BHA-chitosan-ciprofloxacin
glutaraldehyde in phosphate buffer saline Towards
Staphylococcus aureus ATCC25923 for 30 days, Each
value represents the mean + S.D. of 3 determinations
(Hendradi et al, 2015)
0.7%
3.
Poten.y of Cyptofloxqcin released
to
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Harmankaya, N., Karlsson, J., Palmquist, A.,
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193-199
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The result showed
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ly
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ay
in
1e
30
3
tn
or
to
:c
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IGF-1 and BMP-2)
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J
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Releose