Total starch Resistant starch Non-resistant stach

62 Appendix 9. Result of in vitro starch digestibility analysis experiment II Sample Replication TS g100g RS g100g RS TS NRS g100g NRS TS Pea Cake 1 40.92 3.95 9.65 36.97 90.35 2 41.78 4.01 9.59 37.78 90.41 Rice Cake 100:0 1 71.63 0.44 0.61 71.20 99.39 2 75.13 0.35 0.47 74.77 99.53 Rice cake 97.5:2.5 1 71.18 0.37 0.51 70.81 99.49 2 71.85 0.37 0.52 71.48 99.48 Rice cake 95.0:5 1 77.93 0.34 0.44 77.59 99.56 2 77.80 0.36 0.46 77.44 99.54 Rice cake 92.5:7.5 1 80.00 0.32 0.40 79.67 99.60 2 81.05 0.33 0.41 80.72 99.59 Rice cake 90.0:10 1 82.00 0.27 0.33 81.73 99.67 2 83.00 0.28 0.34 82.72 99.66 Values in the parentheses indicate the ratio between rice flour and sticky rice flour in the cake Appendix 10. ANOVA and Duncan`s test results for in vitro starch digestibility experiment II

10.1 Total starch

Univariate Analysis of Variance Tests of Between-Subjects Effects Dependent Variable:VAR00003 Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 172.946 a 4 43.236 29.275 .001 Intercept 59530.545 1 59530.545 4.031E4 .000 total_starch 172.946 4 43.236 29.275 .001 Error 7.385 5 1.477 Total 59710.875 10 Corrected Total 180.330 9 a. R Squared = .959 Adjusted R Squared = .926 Post Hoc Tests total_starch Homogeneous Subsets 63 VAR00003 Duncan total_starch N Subset 1 2 3 Rice cake 97.5:2.5 2 71.5135 Rice cake 100:0 2 73.3808 Rice cake 95:5 2 77.8648 Rice cake 92.5:7.5 2 80.5216 80.5216 Rice cake 90:10 2 82.4995 Sig. .185 .080 .165 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean SquareError = 1.477.

10.2 Resistant starch

Univariate Analysis of Variance Tests of Between-Subjects Effects Dependent Variable:VAR00002 Source Type III Sum of Squares df Mean Square F Sig. Corrected Model .055 a 4 .014 6.571 .032 Intercept 2.030 1 2.030 965.464 .000 resistant_starch .055 4 .014 6.571 .032 Error .011 5 .002 Total 2.096 10 Corrected Total .066 9 a. R Squared = .840 Adjusted R Squared = .712 Post Hoc Tests resistant_starch Homogeneous Subsets VAR00002 Duncan resistant_starch N Subset 1 2 3 Rice cake 90:10 2 .3366 Rice cake 92.5:7.5 2 .4059 .4059 Rice cake 95:5 2 .4522 .4522 .4522 Rice cake 97.5:2.5 2 .5168 .5168 Rice cake 100:0 2 .5414 Sig. .058 .066 .117 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean SquareError = .002. 64

10.3 Non-resistant stach

Univariate Analysis of Variance Tests of Between-Subjects Effects Dependent Variable:VAR00001 Source Type III Sum of Squares df Mean Square F Sig. Corrected Model .055 a 4 .014 6.571 .032 Intercept 99100.830 1 99100.830 4.712E7 .000 non_resistant_starch .055 4 .014 6.571 .032 Error .011 5 .002 Total 99100.896 10 Corrected Total .066 9 a. R Squared = .840 Adjusted R Squared = .712 Post Hoc Tests non_resistant_starch Homogeneous Subsets VAR00001 Duncan non_resistant_starch N Subset 1 2 3 Rice cake 100:0 2 99.4586 Rice cake 97.5:2.5 2 99.4832 99.4832 Rice cake 95:5 2 99.5478 99.5478 99.5478 Rice cake 92.5:7.5 2 99.5941 99.5941 Rice cake 90:10 2 99.6634 Sig. .117 .066 .058 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean SquareError = .002. 65 Appendix 11. Result of in vitro starch digestibility analysis experiment III Sample Cold setting TS g100g RS TS NRS TS Pea cake 6h 25 o C 40.9174 9.6501 90.3499 41.7827 9.5881 90.4119 6h 4 o C 46.7517 7.3555 92.6445 45.7235 7.5565 92.4435 24h 4 o C 43.3897 6.9205 93.0795 43.6165 6.9109 93.0891 Rice cake 6h 25 o C 71.6345 0.6131 99.3869 75.1271 0.4698 99.5302 6h 4 o C 66.7811 0.7287 99.2713 67.8961 0.7280 99.2720 24h 4 o C 59.4111 0.9245 99.0755 58.8896 0.9681 99.0319 Rice cake 90:10 6h 25 o C 82.0019 0.3339 99.6661 82.9971 0.3393 99.6607 6h 4 o C 68.6888 0.4336 99.5664 69.4399 0.4602 99.5398 24h 4 o C 66.9994 0.5839 99.4161 66.5328 0.5325 99.4675 Values in the parentheses indicate the ratio between rice flour and sticky rice flour in the cake TS: total starch, RS: resistant starch, NRS: non-resistant starch Appendix 12. ANOVA and Duncan`s test results for in vitro starch digestibility experiment III

12.1 Resistant starch content of pea cake