ISOLASI DAN UJI MIKROBA ANTAGONIS RIZOSFER POTENSIAL TERHADAP PATOGEN KANKER BATANG TANAMAN APEL (Malus sylvestris Mill)
Undergraduate Theses dari JIPTUMMPP / 2009-07-23 10:34:52
ISOLASI DAN UJI MIKROBA ANTAGONIS RIZOSFER POTENSIAL
TERHADAP PATOGEN KANKER BATANG TANAMAN APEL (Malus
sylvestris Mill)
Oleh: IFTAHUL KARIM ( 04710011 )
Agronomy
Dibuat: 2009-07-23 , dengan 6 file(s).
Keywords: Isolasi, Antagonis dan Patogen, Uji daya hambat
ABSTRAK
Biaya produksi apel yang terus meningkat disebabkan oleh harga pestisida pengendali penyakit
yang semakin mahal. Isolasi mikroba dari rizosfer dapat digunakan untuk menanggulangi
patogen penyebab penyakit kanker batang tanaman apel dengan uji antagonis. Kemampuan daya
hambat adalah nilai kemampuan antagonis dalam menekan pertumbuhan patogen penyebab
penyakit kanker batang.
Tujuan dalam penelitian ini adalah untuk mendapatkan isolat mikroba antagonis rizosfer
potensial untuk menekan pertumbuhan patogen kanker batang tanaman apel (Malus sylvestris
Mill).
Penelitian ini dilaksanakan di Laboratorium Agronomi Fakultas Pertanian Universitas
Muhammadiyah Malang pada Bulan September 2008 sampai bulan Januari 2009 pada suhu ratarata ruang 250 C dengan kelembaban 81 %.
Mikroba hasil isolasi diuji dengan tahapan pengujian: (1) pemurnian mikroba: (1.a) pemurnian
patogen kanker batang dengan mengamati luas permukaan mikroba selama 7 hari, (1.b)
pemurnian mikroba antagonis sebanyak mikroba yang didapat dari rizosfer, (2) uji daya
antagonistik: (2.a) uji mikroba antagonis dengan patogen kanker batang, (3) penghitungan besar
daya hambat antagonis terhadap patogen kanker batang pada hari ke 7 setelah inokulasi.
Berdasarkan hasil penelitian dapat disimpulkan bahwa: (1) Didapat satu patogen penyebab
penyakit kanker batang (Corticium salmonicolor), (2) Didapat 10 (sepuluh) jenis mikroba
antagonis dari lokasi Batu pada tanah perakaran sentra produksi tanaman apel yang terdiri dari 8
isolat jamur dan 2 isolat bakteri, (3) Urutan Isolat antagonis yang mampu menghambat
pertumbuhan patogen pada uji antagonis mulai yang terbesar adalah A1, A4, A7, A5, A3, A6,
A2, A8, A9, dan A10 dengan urutan besar daya hambat 96,61%, 89,84%, 86,35%, 86,32%,
85,69%, 83,58%, 80,02%, 79,43%, 63,36%, dan 37,68%. Hasil penelitian terhadap adanya
penghambatan pertumbuhan patogen oleh mikroba filoplen menunjukan bahwa perlakuan
antagonis mikroba berpengaruh sangat nyata terhadap penghambatan pertumbuhan patogen
(Sartono J.S. dan Sumarmi, 2009).
ABSTRACT
Production cost of apple that more and more increase is caused by pesticide price of disease
controller in which more expensive. Isolation of microbe from rhizosfer can be used to overcome
pathogen as caused of stem cancer disease of apple by antagonist test. Capability of resistivity is
capability value of antagonist to pressure pathogen as caused of stem cancer disease.
The aim of this research is to get isolate of potential rhizosfer antagonist microbe to pressure the
growth of stem cancer pathogen of apple (Malus sylvestris Mill).
The research was conducted in the Agronomy Laboratory of Agriculture Faculty,
Muhammadiyah University of Malang on September 2008 to January 2009 at the average degree
of the room 250 C with humidity 81%.
Microbe of isolation yield was tested by examination phase: (1) purification of microbe: (1.a)
purification of stem cancer pathogen by observe large of microbe surface during 7 days, (1.b)
purification of microbe antagonist as microbe was obtained from rhizosfer, (2) test of
antagonistic power: (2.a) test of microbe antagonist with pathogen of stem cancer, (3) calculation
of resistivity amount toward pathogen of stem cancer at the seven days after inoculation.
Based on research result can be concluded that: (1) Obtained one pathogen as caused of stem
cancer disease (Corticium salmonicolor), (2) Obtained 10 (ten) types of microbe antagonist from
Batu on the root land of production centre of apple consist of 8 isolate mushroom and 2 isolate
bacteria, (3) Sequence of isolate antagonist is able to hamper the growth of pathogen at the test
of antagonist start from the biggest are A1, A4, A7, A5, A6, A2, A8, A9 and A10 with in
sequence of resistivity 96,61%, 89,84%, 86,35%, 86,32%, 85,69%, 83,58%, 80,02%, 79,43%,
63,36% and 37,68%. The research result of the growth obstacle of pathogen by microbe filoplen
indicated that antagonist treatment of microbe has real effect toward the growth obstacle of
pathogen (Sartono J.S and Sumarmi, 2009).
ISOLASI DAN UJI MIKROBA ANTAGONIS RIZOSFER POTENSIAL
TERHADAP PATOGEN KANKER BATANG TANAMAN APEL (Malus
sylvestris Mill)
Oleh: IFTAHUL KARIM ( 04710011 )
Agronomy
Dibuat: 2009-07-23 , dengan 6 file(s).
Keywords: Isolasi, Antagonis dan Patogen, Uji daya hambat
ABSTRAK
Biaya produksi apel yang terus meningkat disebabkan oleh harga pestisida pengendali penyakit
yang semakin mahal. Isolasi mikroba dari rizosfer dapat digunakan untuk menanggulangi
patogen penyebab penyakit kanker batang tanaman apel dengan uji antagonis. Kemampuan daya
hambat adalah nilai kemampuan antagonis dalam menekan pertumbuhan patogen penyebab
penyakit kanker batang.
Tujuan dalam penelitian ini adalah untuk mendapatkan isolat mikroba antagonis rizosfer
potensial untuk menekan pertumbuhan patogen kanker batang tanaman apel (Malus sylvestris
Mill).
Penelitian ini dilaksanakan di Laboratorium Agronomi Fakultas Pertanian Universitas
Muhammadiyah Malang pada Bulan September 2008 sampai bulan Januari 2009 pada suhu ratarata ruang 250 C dengan kelembaban 81 %.
Mikroba hasil isolasi diuji dengan tahapan pengujian: (1) pemurnian mikroba: (1.a) pemurnian
patogen kanker batang dengan mengamati luas permukaan mikroba selama 7 hari, (1.b)
pemurnian mikroba antagonis sebanyak mikroba yang didapat dari rizosfer, (2) uji daya
antagonistik: (2.a) uji mikroba antagonis dengan patogen kanker batang, (3) penghitungan besar
daya hambat antagonis terhadap patogen kanker batang pada hari ke 7 setelah inokulasi.
Berdasarkan hasil penelitian dapat disimpulkan bahwa: (1) Didapat satu patogen penyebab
penyakit kanker batang (Corticium salmonicolor), (2) Didapat 10 (sepuluh) jenis mikroba
antagonis dari lokasi Batu pada tanah perakaran sentra produksi tanaman apel yang terdiri dari 8
isolat jamur dan 2 isolat bakteri, (3) Urutan Isolat antagonis yang mampu menghambat
pertumbuhan patogen pada uji antagonis mulai yang terbesar adalah A1, A4, A7, A5, A3, A6,
A2, A8, A9, dan A10 dengan urutan besar daya hambat 96,61%, 89,84%, 86,35%, 86,32%,
85,69%, 83,58%, 80,02%, 79,43%, 63,36%, dan 37,68%. Hasil penelitian terhadap adanya
penghambatan pertumbuhan patogen oleh mikroba filoplen menunjukan bahwa perlakuan
antagonis mikroba berpengaruh sangat nyata terhadap penghambatan pertumbuhan patogen
(Sartono J.S. dan Sumarmi, 2009).
ABSTRACT
Production cost of apple that more and more increase is caused by pesticide price of disease
controller in which more expensive. Isolation of microbe from rhizosfer can be used to overcome
pathogen as caused of stem cancer disease of apple by antagonist test. Capability of resistivity is
capability value of antagonist to pressure pathogen as caused of stem cancer disease.
The aim of this research is to get isolate of potential rhizosfer antagonist microbe to pressure the
growth of stem cancer pathogen of apple (Malus sylvestris Mill).
The research was conducted in the Agronomy Laboratory of Agriculture Faculty,
Muhammadiyah University of Malang on September 2008 to January 2009 at the average degree
of the room 250 C with humidity 81%.
Microbe of isolation yield was tested by examination phase: (1) purification of microbe: (1.a)
purification of stem cancer pathogen by observe large of microbe surface during 7 days, (1.b)
purification of microbe antagonist as microbe was obtained from rhizosfer, (2) test of
antagonistic power: (2.a) test of microbe antagonist with pathogen of stem cancer, (3) calculation
of resistivity amount toward pathogen of stem cancer at the seven days after inoculation.
Based on research result can be concluded that: (1) Obtained one pathogen as caused of stem
cancer disease (Corticium salmonicolor), (2) Obtained 10 (ten) types of microbe antagonist from
Batu on the root land of production centre of apple consist of 8 isolate mushroom and 2 isolate
bacteria, (3) Sequence of isolate antagonist is able to hamper the growth of pathogen at the test
of antagonist start from the biggest are A1, A4, A7, A5, A6, A2, A8, A9 and A10 with in
sequence of resistivity 96,61%, 89,84%, 86,35%, 86,32%, 85,69%, 83,58%, 80,02%, 79,43%,
63,36% and 37,68%. The research result of the growth obstacle of pathogen by microbe filoplen
indicated that antagonist treatment of microbe has real effect toward the growth obstacle of
pathogen (Sartono J.S and Sumarmi, 2009).