Analisis QTL sifat ketahanan penyakit BLAS (Pyricularia grisae, Sacc) pada populasi hasil persilangan IR64 dengan spesies padi liar Oryza rufipogon, Griff.
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ABSTRACT
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Dwinita W. Utami. QUANIT~ATIYE
Trait Loci (QTL) Analysis of Bhst
(Pydcdda g r k a , Sam) Resistance in Popuhtian Derived from Crossing
between IR64 and Wild Rice Species, Orym -on,
Griti) mder the direction
of HAJRZAL ASWIDINNOOR, ED1 GUHARDJA, ASEP SETIAWAN, and
SUGIONO MOEWOPAWIRO.
2 g= =g Sw O
s
t
rz
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asgo
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Wild spcies of rice are the one of unique sources of genetic variation, which
P,
can be used for genehc improvement especially for quantitative traits. Blast disease
,
i
,I
caused by pv'iculariu grhea,Sacc, could cawe high percentage of yield losses on
pduction. The improvement of blast resistance in rice is difficult due to the
8 rice
complexity
of the idmiof this trait as well as the dynamic of the pathogen.
3.
Wild s p i e s of Orym can be exploited to emich the blast mistance controlled by
~ f S quantitative trait loci (QTL) in the existing wieties. Oyza &pgon, Griff (AA
Acc No. IRGC#105491), om of the wild rice species, has ban indicating to
=
' genome;
contab QTL of blast in BC& population of cross with IR64. Marker assisted
selection (MAS), therefore, could k osd to s p e d up the -cry of recurrent parent
S
getoorneia~~~The~~erallobjectiveofthis~hwasto~yze
tbe blast QTL wighated froIll interspeslfic muss between IR64 and wild rice spies,
0 . d n based on genetic, htrogression and mapping a d y s ~ s .This study was
2.
exp&d to &st the M n g program for tbe development of new blast mistant
ricelines.
o
o
This study consisted of 3 experiments. First: Analysis of blast resistance
inhexito dctcd genes action wming h r n 0.nJflpgon oontribvhng to the
resisEaace of three blast t ~ l ~ eTwo
~ . pamb (PI: R 64 and Pa: 0.M p g o n ) , F1, Fz,
B(=Pt and BCfi ~
o n wen
s s c a d on the basis of disease leaf ares (O/&M)
rwulting h m tht three bIast races inoculation in gmxbuse. Tht genetic a
m
d
m of
this study consisted: m a n peration analysis, ckcmhtiofl of genetic models and
~
O
Z the Ivariance compmmt and heritability. Tbe d i n g t&
evaluated tbe
g o a k w of fit of the three parameters m h l (mid pmb [ml additive [dl and
dominance [h] effect). When the
madel did not show a good fit, a
six-pramdm joint scaling testwas usedto d e b m h the adequaq of a digmic
epistatic m o d , which provide eshates of the three qmtatic pameten: additive x
[A
0
additive [iJ, additive x dominance tj] and dominance x
,
a
"
~~
*>
1.
0
r
-
F
3
C
s
7'
(D
3
-.
u^
wild rice s p i e s 0. ngipogon- T'he MAS technique was used to detect the
O.n&gon
in-on
into IR64 in X 2 F 3 population. Third: QTL analysis for
blast resistance on selected lines (fmm Exp. 2). Twenty selected lines were multiplied
iadividualIy to generate a population and used for blast QTL mapping based on
statktical approach of s u e point Bnalpk (SPA), interval mrlpping (IM)and
composite interval mapping (CIM).
d
p u p 4!
gaai
huuZ
ABSTRACT
s s
""3 1
s w
3
o w
n s
0 ,
s s 0 r
0
Dwinita W. Utami. QUANIT~ATIYE
Trait Loci (QTL) Analysis of Bhst
(Pydcdda g r k a , Sam) Resistance in Popuhtian Derived from Crossing
between IR64 and Wild Rice Species, Orym -on,
Griti) mder the direction
of HAJRZAL ASWIDINNOOR, ED1 GUHARDJA, ASEP SETIAWAN, and
SUGIONO MOEWOPAWIRO.
2 g= =g Sw O
s
t
rz
B
asgo
g2su22=
S S Q S
Q
Qcn.lrt
9 o"g9 5
3S$@'!?.
a! jzzgt vg.ac
.
, 0
I
msml
~ " sn
n
z
%
0
c
0-0 X T C
s
f i ,a,
4.
E
. -9 E *
n -n. a 3.
f 9 g g
~
E
s "EX
g
9;
2 ilg
3
3=
T
rr
-.
v.
55
9%
9 t
C Q
z: z.9
i.2
7.~3
8
Q
P $3 i5%
$
gg
n
ZE
=I
s
8s
r
X
= 3
FW
$ i!
.5
B
"
i?
=9'
t
g
E
is!
g
-.
f
9
E
5
3
s
!3+
Wild spcies of rice are the one of unique sources of genetic variation, which
P,
can be used for genehc improvement especially for quantitative traits. Blast disease
,
i
,I
caused by pv'iculariu grhea,Sacc, could cawe high percentage of yield losses on
pduction. The improvement of blast resistance in rice is difficult due to the
8 rice
complexity
of the idmiof this trait as well as the dynamic of the pathogen.
3.
Wild s p i e s of Orym can be exploited to emich the blast mistance controlled by
~ f S quantitative trait loci (QTL) in the existing wieties. Oyza &pgon, Griff (AA
Acc No. IRGC#105491), om of the wild rice species, has ban indicating to
=
' genome;
contab QTL of blast in BC& population of cross with IR64. Marker assisted
selection (MAS), therefore, could k osd to s p e d up the -cry of recurrent parent
S
getoorneia~~~The~~erallobjectiveofthis~hwasto~yze
tbe blast QTL wighated froIll interspeslfic muss between IR64 and wild rice spies,
0 . d n based on genetic, htrogression and mapping a d y s ~ s .This study was
2.
exp&d to &st the M n g program for tbe development of new blast mistant
ricelines.
o
o
This study consisted of 3 experiments. First: Analysis of blast resistance
inhexito dctcd genes action wming h r n 0.nJflpgon oontribvhng to the
resisEaace of three blast t ~ l ~ eTwo
~ . pamb (PI: R 64 and Pa: 0.M p g o n ) , F1, Fz,
B(=Pt and BCfi ~
o n wen
s s c a d on the basis of disease leaf ares (O/&M)
rwulting h m tht three bIast races inoculation in gmxbuse. Tht genetic a
m
d
m of
this study consisted: m a n peration analysis, ckcmhtiofl of genetic models and
~
O
Z the Ivariance compmmt and heritability. Tbe d i n g t&
evaluated tbe
g o a k w of fit of the three parameters m h l (mid pmb [ml additive [dl and
dominance [h] effect). When the
madel did not show a good fit, a
six-pramdm joint scaling testwas usedto d e b m h the adequaq of a digmic
epistatic m o d , which provide eshates of the three qmtatic pameten: additive x
[A
0
additive [iJ, additive x dominance tj] and dominance x
,
a
"
~~
*>
1.
0
r
-
F
3
C
s
7'
(D
3
-.
u^
wild rice s p i e s 0. ngipogon- T'he MAS technique was used to detect the
O.n&gon
in-on
into IR64 in X 2 F 3 population. Third: QTL analysis for
blast resistance on selected lines (fmm Exp. 2). Twenty selected lines were multiplied
iadividualIy to generate a population and used for blast QTL mapping based on
statktical approach of s u e point Bnalpk (SPA), interval mrlpping (IM)and
composite interval mapping (CIM).