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Alveolus conggestion
Thickening of the alveolus
mass due to necrosis
Figure 2 : Normal Lung Cells Figure 3 : Lung cells induced by DMBA dose of 20 mg kg
Thickening of the alveolar septa periatritis it is suspected inflammation as early cancer formation. The conggesti outside of the alveoli red indicates that the blood circulation is not smooth. Necrosis
characterized by mass outside of the alveoli. This necrosis caused by the presence of compounds DMBA induced. DMBA is a carcinogen which is activated by cytochrome P450 enzymes to become active as
epoksid reactive compounds to bind to DNA Wulan PR, et al, 2012.
The thickening is reduced
Alveolus getting better
Figure 4. Lung cell induced by DMBA dose of 20 mg kg and treated with sarangsemut extracts dose of 750 mg kg oral injection
From the above picture in general has undergone many changes. Conggestion is diminishing and alveolar became thinning, approaching normal. In general it can be stated that at a dose of 750mg kg Sarangsemut
extract can improve the condition of the lung cells that have been damaged by DMBA induced.
4. Conclusion
a. Comparison 1:10 the amount of solvent extraction, time of 50 minutes has the best yield ie 14 b. The same time of number and ratio solvent extraction has the highest IC
50
47.828 ppm
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c. Fraction of water plants Sarangsemut has the potential as an anticancer drug. d. Sarangsemut extract dose of 750 mg kg of body weight was well enough in the treatment of rats
that suffered damage lung cells. Acknowledgment
Thank you to DITLITABMAS-DIKTI through Kopertis VI Central Java who have financed research in ―Hibah Pekerti‖ scheme.
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DNA Repair and Mutagenesis American society and Microbiology.
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Characteristics of Mesopore Silica Synthesized Using Bovine Bone Gelatin as A Template
P M Pattiasina
1
, W Trisunaryanti
2
, I I Fallah
2
, Sutarno
2
, I Kartini
2
, and K Wijaya
2
1
Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Pattimura, Ambon 97233, Republic of Indonesia
2
Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Gadjah Mada, Jl Sekip Utara 15, Yogyakarta 55281, Republic of Indonesia
E-mail: wegatriyahoo.com
Abstract. The utilization of bovine bone gelatin as a template for the synthesis of mesopore silica by hydrothermal and sonochemical methods have been studied. The gelatin was obtained from bovine
bond by treatment using NaOH, citric acid, and HCl for 24 h followed by hydrolysis at 80 °C. The synthesis of mesopore silica was conducted under hydrothermal and sonochemical methods. The
gelatin was analyzed by FT-IR and the mesopore silica was characterized by Surface Area Analyzer and TEM. The results showed that the gelatin consisted of Amida A, Amida I, II and III. The two
methods produced silica material with meso scale pore diameter and showed wormhole-like pores shape material in their TEM images. The hydrothermally synthesized silica has a pore diameter of
5.14 nm with surface area of 356.18 m2g, and that of the sonochemically synthesized silica has a pore diameter of 3.13 nm and surface area of 451.39 m2g.
1. Introduction Template is the main thing in mesoporous silica synthesis process. A non ionic template like gelatin
showed a good biocompatability, non toxic, and biodegradable [1]. Gelatin is a polypeptide, a nature polymer of collagen. It contains a lots of N-H functional groups which trends to strongly interact with
silanol groups Si-OH on the silicate species via multiple hydrogen bonds. There are few studies that investigated the utilization of gelatin as a template [1-3]. However, the gelatin used was still limited as a
synthetic gelatin. Therefore, better idea is to use gelatin from bovine bone as a template. Bovine bone is a waste and contains a lot of collagen, so it can be used to produce gelatin. The most commonly reported
method for the synthesis of mesoporous materials is a hydrothermal method [1, 2, 4]. This method can produce mesoporous materials with high pore diameter. But, its greenless because needs high temperatur
when synthesis proccess. In order to avoid this disadvantage, there is a green method that can be used is sonochemical method [5]. Sonochemical method performed at room temperatur using ultrasonic wave
with frequency of above 20 kHz.
Based on the above consideration the authors undertaken to synthesis mesoporous silica with bovine bone gelatin as a template using hydrothermal and sonochemical methods. The results were
discussed below.
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2. Materials and Methods
