10th Joint Conference on Chemistry Surakarta, September 8
th
–9
th
, 2015 Proceeding of Chemistry Conferences vol. 1 2016
ISSN 2541-108X
14
2. Materials and Methods
2.1. Materials
Bovine bone were obtained from Jangkang, Widodomartani market, Ngemplak, Sleman, Yogyakarta. HCl, NaOH, citric acid, H2SO4, Na2SiO3.
2.2. Instruments
Fourier Transform Infrared Spectroscopy FTIR, Shimadzu Prestige FTIR 21, Surface Area Analyzer SAA, Quantachrome NovaWin2, Transmission Electron Microscope TEM, JEOL JEM-1400.
2.3. Extraction of gelatin
Bones were washed with tap water to remove superfluous material and degreasing at 80 °C. After that, the bovine bone were treated with NaOH 4 for 1, 12, and 24 h to remove the non-collageneous protein.
They were washed thoroughly with tap water, and treated with citric acid 1,2 for 1, 12, and 24 h, followed by washed with tap water and treated with HCl solution 4 for 1, 12, and 24 h to increase
swelling. The final extraction was carried out with distilled water at weight ratio of waterbone = 3 at 60 and 80 °C for 5 h. The extract was filtered through Whattman No.42 filter paper under vacuum. The yield
of the gelatin was characterized using FTIR.
2.4. Synthesis of mesoporous silica
Gelatin with distribution value of molecular weight around 120-160 kD was used as a template in synthesis of mesoporous silica. Typical synthesis procedure for the mesoporous silicas with bovine
bone gelatin as template using hidrotermal method according to the method of Hsu et al. [2] with some modifications as following: 1.0 g of gelatin was dissolved in 25.0 g of water to form a clear
solution. A solution of a 4.0 g of sodium silicate and a 25.0 g of water added with a drop of 0.1 M H
2
SO
4
until pH value was adjusted to about 5.0 at 40°C was prepared. Then, the gelatin solution was poured directly into the silicate solution under stirring and precipitate was generated
within seconds. After stirring for 1 h, the pH value of the gel solution was adjusted to 6.0-3.0. Finally, the gel solution was transferred into an autoclave, and hydrothermally treated at 100°C for
24 h. The solid obtained was then washing, drying and calcining at 550°C produced the mesoporous silica. The mesoporous silica was characterized using FTIR, GSA, TEM. Typical synthesis procedure for
the mesoporous silicas with bovine bone gelatin as a template using sonochemical method was carried out in the same procedure as the hydrothermal method but the step of hydrothermally treated was changed
into sonication using sonicator for 90 min.
3. Results and discussion
