10th Joint Conference on Chemistry Surakarta, September 8
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, 2015 Proceeding of Chemistry Conferences vol. 1 2016
ISSN 2541-108X
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From Table 1 it can be stated that the number of solvent 10 times with a time of 50 minutes produced the greatest yield. Whereas in previous studies Suharyanto 2012 by maceration method with a time of 5 days
or 120 hours produced only 10 yield. At maceration method the opening of the cell nucleus is much less effective than the ultrasonic method. The yield difference was due to the timing and amount of solvent. In
general, the major solvent and time increased causing the yield to rise, but at a certain point where saturation occurs a decline in the yield. In the solvent ratio 1:10 with a time of 60 minutes a decline in the
yield of this possible dissolution process occurs at the saturation point.
3.2 Antioxidan Activity The results of antioxidant activity test is presented in table 2
Table 2 Antioxidan Activity results of water fractions No. Time
Equations IC
50
ppm Conclusion
1. 630 Y=0.126X + 10.50
313.492 weak
2. 640 Y = 0.168X+12.16
225.239 weak
3. 650 Y=0.440X -2.143
118.492 medium
4. 660 Y=0.3X+ 31.51
61.630 strong
5. 830 Y=0.114X+2.292
418.412 weak
6. 840 Y=0.315X+11.25
123.0158 medium
7. 850 Y=0.277X+29.76
73.0685 strong
8. 860 Y=0.64X+6.645
67.7421 strong
9. 1030 Y=0.225X + 12.97
164.578 medium
10. 1040 Y=0.183X+21.15
157.650 medium
11. 1050 Y=0.173X+16.65
47.28 very strong
12. 1060 Y=0.093X+19.55
327.419 weak
From table 2 can be stated that the sample with the extraction time of 50 minutes with 10 times the amount of solvent has the best antioxidant activity with IC
50
47.828 ppm.In the previous research produced IC
50
of 37.5 ppm with maceration method, whereas the ultrasonic method produced greater
yields with shorter time. 3.3 Hystophatologi
Histopathology test results of rats are presented in the following figures:
10th Joint Conference on Chemistry Surakarta, September 8
th
–9
th
, 2015 Proceeding of Chemistry Conferences vol. 1 2016
ISSN 2541-108X
11
Alveolus conggestion
Thickening of the alveolus
mass due to necrosis
Figure 2 : Normal Lung Cells Figure 3 : Lung cells induced by DMBA dose of 20 mg kg
Thickening of the alveolar septa periatritis it is suspected inflammation as early cancer formation. The conggesti outside of the alveoli red indicates that the blood circulation is not smooth. Necrosis
characterized by mass outside of the alveoli. This necrosis caused by the presence of compounds DMBA induced. DMBA is a carcinogen which is activated by cytochrome P450 enzymes to become active as
epoksid reactive compounds to bind to DNA Wulan PR, et al, 2012.
The thickening is reduced
Alveolus getting better
Figure 4. Lung cell induced by DMBA dose of 20 mg kg and treated with sarangsemut extracts dose of 750 mg kg oral injection
From the above picture in general has undergone many changes. Conggestion is diminishing and alveolar became thinning, approaching normal. In general it can be stated that at a dose of 750mg kg Sarangsemut
extract can improve the condition of the lung cells that have been damaged by DMBA induced.
4. Conclusion
