g Zn-ZnSO g Zn-ZNSO g Zn-EDTA kg seed 2.8 g Zn kg seed

154 AGRONOMY JOURNAL, VOL. 93, JANUARY–FEBRUARY 2001 Table 4. Effect of Zn seed treatment source and rate on rice total Table 2. Effect of rice cultivar on grain yield, total dry matter TDM, and harvest moisture averaged across Zn treatments dry matter TDM by 14 d after flooding compared with an untreated and standard check at two locations in 1999. during 1998 at the Rice Research and Extension Center RREC, near Stuttgart. TDM† Cultivar Grain yield TDM Grain moisture Zn fertilizer treatment PTBS‡ RREC§ kg ha 2 1 g kg 2 1 kg ha 2 1 Drew 4838 428 210 Control 659 377 Cypress 4435 358 230 11 kg Zn ha 2 1 PPI¶ 708 1007 Jefferson 3125 275 188 1.0 g Zn-ZnSO 4 kg seed 2 1 986 820 LSD0.05 353 95 21

2.2 g Zn-ZnSO

4 kg seed 2 1 1053 1056

4.7 g Zn-ZNSO

4 kg seed 2 1 1181 1111 Significant at the 0.05 level. 1.4 g Zn-EDTA kg seed 2 1 849 562 Significant at the 0.001 level. 2.8 g Zn-EDTA kg seed 2 1 1193 955

5.7 g Zn-EDTA kg seed

2 1 1147 1232 fertilizer treatment interaction but were significantly LSD0.10 within location 136 LSD0.10 between locations 193 different among cultivars Table 2. The lack of differ- ences among the Zn fertilizer treatments suggested that † P-value for Zn fertilizer treatment 3 location 5 0.092. ‡ PTBS, Pine Tree Branch Station, Colt, AR. rice was not Zn deficient and would not show a signifi- § RREC, Rice Research and Extension Center, near Stuttgart, AR. cant yield and growth response to Zn fertilization. Al- ¶ Standard check. PPI, preplant incorporated. though not statistically significant at the 0.05 level of Seeding rate for all treatments was 120 kg ha 2 1 seed. probability P 5 0.16, the grain yield for both 11 kg Zn ha 2 1 PPI and 2.8 g Zn kg seed 2 1 was 214 and 360 plots while few or no symptoms were observed in the kg ha 2 1 greater than the control, respectively, when 4.7 g Zn kg seed 2 1 as ZnSO 4 and 5.7 g Zn kg seed 2 1 averaged across the cultivars Table 3. as ZnEDTA plots. The deficiency symptoms were most The tissue Zn concentration of whole-plant seedlings severe at the RREC. Thus, the response of the rice 14 d after flooding did show significant differences be- TDM among Zn treatments differed between locations tween Zn fertilizer treatments Table 3. The tissue Zn for some treatments Table 4. The rice TDM response concentration followed the highest-to-lowest order of at each location was indicative of the degree of Zn 11 kg Zn ha 2 1 PPI . 2.8 g Zn kg seed 2 1 . control. deficiency symptoms expressed among Zn treatments Rasmussen and Boawn 1969 suggested that Zn seed at each location. treatment of kidney bean was not an adequate alterna- At the PTBS, seeds that were treated with Zn pro- tive to broadcast Zn fertilizer applications to the soil duced significantly greater TDM by 14 d after flooding because Zn deficiency symptoms occurred, and Zn seed than both the control and 11 kg Zn ha 2 1 PPI, regardless treatment failed to produce yields equal to soil Zn appli- of the Zn source or rate. It is unclear why Zn deficiency cation. Based on the 1998 tissue concentration data, 11 symptoms occurred in the 11 kg Zn ha 2 1 PPI treatment. kg Zn ha 2 1 PPI was superior to 2.8 g Zn kg seed 2 1 The random occurrence of deficiency symptoms within for supplying Zn to seedling rice plants. However, both each 11 kg Zn ha 2 1 PPI plot suggests that uniform fertil- treatments increased tissue Zn in rice seedlings above izer distribution at the applied rate could not supply the the level considered deficient 20 mg kg 2 1 while the plants that were physically located between fertilizer control was near the critical threshold Sedberry et al., granules with adequate Zn nutrition. Both the control 1987. Although Zn tissue concentration data were a and 11 kg Zn ha 2 1 PPI treatments recovered from the useful means of evaluation, the effectiveness of Zn seed treatments can best be made under Zn deficient con- early Zn deficiency symptoms within 14 d after plant ditions. samples were taken. Within the Zn seed treatments, the TDM tended to increase as the Zn application rate in- 1999 Experiments creased. At the RREC, the TDM for 11 kg Zn ha 2 1 PPI was Zinc deficiency symptoms similar to those described significantly greater than the control, 1.0 g Zn kg by Sedberry et al. 1978 were observed at both locations seed 2 1 as ZnSO 4 , and 1.4 g Zn kg seed 2 1 as ZnEDTA in 1999. The symptoms were most severe in the control treatments and equal to all other treatments. Stand loss Table 3. Effect of Zn fertilizer treatment, averaged across culti- occurred in two of four replications of the control plots vars, on grain yield and tissue Zn concentration of seedling at the RREC and resulted in a significantly lower TDM rice 14 d after flooding during 1998 at the Rice Research and than the control at the PTBS. The only other treatments Extension Center RREC, near Stuttgart. that showed significant differences between locations Tissue Zn were the 11 kg Zn ha 2 1 PPI and 1.4 g Zn kg seed 2 1 Zn fertilizer treatment Grain yield concentration as ZnEDTA treatments. It is unclear why the 11 kg Zn kg ha 2 1 mg kg 2 1 ha 2 1 PPI treatment responded differently between the Control 3929 19.7 two locations. The 1.4 g Zn kg seed 2 1 as ZnEDTA 11 kg Zn ha 2 1 PPI† 4143

31.6 2.8 g Zn kg seed

2 1 ‡ 4289 24.4 and 1.0 g Zn kg seed 2 1 as ZnSO 4 treatments apparently LSD0.05 NS§ 2.6 contained insufficient Zn to maximize plant growth at Significant at the 0.001 probability level. the RREC. Based on the TDM data from both locations, † PPI, preplant incorporated. Zn seed treatments should be applied at rates between ‡ Seeding rate for all treatments was 120 kg ha 2 1 seed. § NS, not significant at the 0.05 level of probability. P 5 0.16. 2.2 and 5.8 g Zn kg seed 2 1 for optimum growth under SLATON ET AL.: EVALUATION OF ZINC SEED TREATMENTS FOR RICE 155 Table 6. Effect of Zn seed treatment source and rate on rice grain Table 5. Effect of Zn seed treatment source and rate on rice tissue Zn concentration by 14 d after flooding compared with yield and harvest grain moisture compared with an untreated and standard check in 1999 data averaged across two lo- an untreated and standard check at two locations in 1999. cations. Tissue Zn concentration Zinc treatment Grain yield Grain moisture Treatment PTBS† RREC‡ kg ha 2 1 g kg 2 1 mg kg 2 1 Control 6154 198 Control 17.5 17.5 11 kg Zn ha 2 1 PPI† 7590 187 11 kg Zn ha 2 1 PPI§ 19.4

31.0 1.0 g Zn-ZnSO

4 kg seed 2 1 ‡ 7051 179 1.0 g Zn-ZnSO 4 kg seed 2 1 ¶ 20.2

16.9 2.2 g Zn-ZnSO