29
V. KESIMPULAN DAN SARAN
5.1 Kesimpulan
Dari hasil penelitian dapat disimpulkan bahwa : 1.
Promoter β-aktin ikan mas telah berhasil diisolasi dengan panjang 1,5 kbp dan yang digunakan dalam pembuatan konstruksi adalah 1,3 kbp. Pada sekuens
yang diisolasi terdapat faktor transkripsi yang biasa dijumpai pada promoter β- aktin, yaitu boks TATA, motif CCAAT dan motif CArG CCAT
6
GG. 2.
Promoter homolog memiliki efektivitas yang lebih tinggi daripada promoter heterolog pada transgenesis ikan mas.
5.2 Saran
Promoter β-aktin ikan mas sebaiknya digunakan dalam memproduksi ikan
mas transgenik untuk tujuan akuakultur.
30
DAFTAR PUSTAKA
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34 Lampiran 1. Derajat kelangsungan hidup embrio DKH-e, derajat penetasan
DP, persentase embrio yang mengekspresikan gen GFP PEMG dan persentase larva yang mengekspresikan gen GFP pada ikan mas
untuk tiap ulangan.
Keterangan : n
= jumlah embrio yang diinjeksi butir DKH-e = Derajat Kelangsungan Hidup Embrio
DP = Derajat Penetasan
PEMG = Persentase Embrio yang Mengekspresikan Gen GFP PLMG = Persentase Larva yang Mengekspresikan Gen GFP
Jenis Promoter
Ulangan n
DKH-e DP
PEMG PLMG
ccba
1 30
18 60,00
11 36,67
6 20,00
4 13,33
2 30
24 80,00
16 53,33
6 20,00
6 20,00
Rata-rata 30
21 70,00
13,5 45,00
6 20,00
5 16,67
Standar Deviasi
- 4,2
14,14 3,5
11,79 0,0
0,00 1,4
4,71
mba
1 30
20 66,67
15 50,00
16 53,33
4
13,33
2 30
13 43,33
12 40,00
5 16,67
3 10,00
Rata-rata 30
16,5 55,00 13,5
45,00 10,5
35,00 3,5
11,67 Standar
Deviasi -
4,9 16,50
2,1 7,07
7,8 25,93
0,7 2,36
tiba
1 30
14 46,67
5 16,67
3 10,00
0,00
2 30
10 33,33
2 6,67
2 6,67
0,00
Rata-rata 30
12 40,00
3,5 11,67
2,00 8,33
0,0 0,00
Standar Deviasi
- 2,8
9,43 2,1
7,07 1,4
2,36 0,0
0,00
kontrol
1 30
22 73,33
19 63,33
0,00
0,00
2 30
25 83,33
20 66,67
0,00
0,00
Rata-rata 30
23,5 78,33 19,5
65,00 0,00
0,00 Standar
Deviasi
-
2,1 7,07
0,7 2,36
0,0 0,00
0,0 0,00
35 Lampiran 2. Tingkat ekspresi gen green fluorescent protein pada embrio ikan
mas untuk tiap ulangan.
Jenis Promoter
Ulangan Tingkat Ekspresi Gen
Jml embrio yg terekspresi
1 2
3 Butir
Butir Butir
Butir
ccBA 1
3 10
2 6,67
1 3,33
6 2
2 6,67
3 10
1 3,33
6 Rata-rata
2,5 8,33
2,5 8,33
1 3,33
6 Standar Deviasi
0,7 2,4
0,7 2,4
0,0 0,0
0,0 mkBA
1 6
20 6
20 4
13,33 16
2 2
6,67 2
6,67 1
3,33 5
Rata-rata 4,0
13,3 4
13,3 2,5
8,3 10,5
Standar Deviasi 2,8
9,4 2,8
9,4 2,1
7,1 7,8
tiBA 1
2 6,67
1 3,33
3 2
1 3,33
1 3,33
2 Rata-rata
1,5 5
1 3,33
2,5 Standar Deviasi
0,7 2,4
0,7 0,0
0,0 0,7
Keterangan : 1. Pendar hijau kurang terang. 2. Pendar hijau terang. 3. Pendar hijau sangat terang.
1
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