Efek pemberian ekstrak daun mahkota dewa (Phaleria macrocarpa) terhadap pertumbuhan sel-sel otak besar anak tikus secara in vitro
EFEK PEMBERIAN EKSTRAK DAUN MAHKOTA DEWA
(Phaleria macrocarpa) TERHADAP PERTUMBUHAN SEL-SEL
OTAK BESAR ANAK TIKUS SECARA IN VITRO
ANI MURTISARI
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
BOGOR
2011
ABSTRACT
ANI MURTISARI. The Effect of “Mahkota Dewa” Leaf Extracts (Phaleria
macrocarpa) on the Newborne Rat Cerebrum Cells In Vitro Growth. Under
direction of ITA DJUWITA and MIN RAHMINIWATI.
Research has been conducted on in vitro culture of three days old rat
(Sprague Dawley) cerebrum cells in DMEM (Dulbecco’s Modified Eagle’s
Medium) containing 10% NBCS (Newborne Calf Serum) and 50 µg/mL
gentamycin (mDMEM), with and without mahkota dewa leaf extracts (MD).
There are five groups of treatment consisted of positive control (mDMEM+30
µg/mL asiaticoside (AC)), negative control (mDMEM), concentration 1
(mDMEM+100 ppm MD), concentration 2 (mDMEM+200 ppm MD), and
concentration 3 (mDMEM+400 ppm MD). Culture was done in 5% CO2 incubator
at 37oC for six days. The parameters observed were Population Doubling Time
(PDT), neuron and glia composition, and the length of axon and dendrite, were
done based on calculation using hemocytometer, Hematoxylin Eosin (HE)
staining, and measured using micrometer, respectively. Data were analyzed using
ANOVA and Duncan. The results showed that mahkota dewa leaf extracts
concentration 400 ppm inhibited the neuronal cells proliferation (P
(Phaleria macrocarpa) TERHADAP PERTUMBUHAN SEL-SEL
OTAK BESAR ANAK TIKUS SECARA IN VITRO
ANI MURTISARI
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
BOGOR
2011
ABSTRACT
ANI MURTISARI. The Effect of “Mahkota Dewa” Leaf Extracts (Phaleria
macrocarpa) on the Newborne Rat Cerebrum Cells In Vitro Growth. Under
direction of ITA DJUWITA and MIN RAHMINIWATI.
Research has been conducted on in vitro culture of three days old rat
(Sprague Dawley) cerebrum cells in DMEM (Dulbecco’s Modified Eagle’s
Medium) containing 10% NBCS (Newborne Calf Serum) and 50 µg/mL
gentamycin (mDMEM), with and without mahkota dewa leaf extracts (MD).
There are five groups of treatment consisted of positive control (mDMEM+30
µg/mL asiaticoside (AC)), negative control (mDMEM), concentration 1
(mDMEM+100 ppm MD), concentration 2 (mDMEM+200 ppm MD), and
concentration 3 (mDMEM+400 ppm MD). Culture was done in 5% CO2 incubator
at 37oC for six days. The parameters observed were Population Doubling Time
(PDT), neuron and glia composition, and the length of axon and dendrite, were
done based on calculation using hemocytometer, Hematoxylin Eosin (HE)
staining, and measured using micrometer, respectively. Data were analyzed using
ANOVA and Duncan. The results showed that mahkota dewa leaf extracts
concentration 400 ppm inhibited the neuronal cells proliferation (P