Introduction Respiration Rate and Respiratory Quotient of Harvested Oyster Mushrooms at Different Storage Temperatures.

2

1. Introduction

Some agricultural produces are perishable that characterized by their short shelf life. Physical, chemical or biological damages on product accelerate the deterioration of products. The improper management of storage conditions, i.e., too warm or too cold for a certain product, is another factor that increases physiological deterioration. The shelf life of certain products could be extended by applying the proper packaging system also. Temperature and humidity were essential factors for fresh products shelf-life- extension-efforts. Respiration rate which indicates many metabolic activities of the tissues as well as the rate of quality degradation of stored products could be suppressed through lowering the storage temperature to slightly above the critical temperature of certain product Saltveit, 2004. The second factor that has great influence on product quality is humidity. Weight loss can be controlled by reducing the gradient of water vapor pressure WVP between products surface and surrounding atmosphere Perkins et al, 2008. Losing 1-2 of water of produce can reduce sales appeal of produce of fresh produce Kader, 2002. Therefore, the packaging system that can maintain the oxygen and carbon dioxide at certain concentration as well as humidity is needed. The characteristics of packaging system have strong relationship with characteristics of packed product. It has been proved that every product has their own unique properties; therefore, designing of packaging system should be based on those properties. The initial knowledge that is important on designing packaging system is respiration rate on different temperature storage. Respiration rate is main factor that changes the composition of atmosphere dynamically on jar’s head space. The gases concentration inside jar’s head space is always changing dynamically until the equilibrium condition is obtained. This equilibrium condition reached when the respiration rate, transfer of gas oxygen and carbon dioxide were equal. Oxygen that consumed by packed products was compensated by oxygen permeating from the surrounding atmosphere. In the same way to oxygen, the carbon dioxide which diffuses to surrounding atmosphere is replaced by diffusion from product’s tissues. On one hand, oxygen must be available for respiration to avoid anaerobic respiration during storage. On the other hand, over supply of oxygen makes the packaging has no benefit for prolonging shelf life. The critical threshold of gas concentration is considered to design the magnitude of gas transfer of packaging film Beaudry et al., 1992. The water vapor that produced from respiration and transpiration continuously adds the water vapor contained in jar’s head space. Water vapor diffuses to ambient environment with a certain magnitude. Most of them condense on product’s or packaging’s surface when saturated water vapor pressure at certain temperature is exceeded. This liquid water encourages the microbial development and reduces the diffusivity of packaging film. Mushroom is one of the vegetables that have short shelf life, only 3 days on ambient temperature Czapski and Szudyga, 2000. Their dermal tissues have no cuticle to protect them from physical damages or microbial attack or water loss Martine et al., 2000. High moisture content in their fruit body’s makes mushrooms respire and transpire with high rate. Mushroom need low temperature storage 0 - 2 C, low O 2 concentration and relatively high CO 2 concentration as well as high humidity 90-98 Thompson et al, 2002. Oyster Mushroom Pleurotus ostreatus becomes commercial product that is highly demanded nowadays in Yogyakarta. Local farmer and seller pack the mushroom with commercial plastic bag which was available on traditional market. It was needed physiological properties data 3 of Oyster mushroom, that were still rare, to choice the right kind of bag plastic or to design the proper packaging system. Therefore, the aim of this work is to study the physiological behaviors of Oyster mushroom on several variations of temperature storage and gas concentration.

2. Theoretical Approach