CHANGES OF TEXTURE AND RESPIRATION RATE OF SALAK FRUIT IN MODIFIED ATMOSPHERE USING POLYETHYLINE PLASTIC PACKAGING AT VARIOUS PERFORATION.

4t h Int ernat ional Conference on Biosciences and Biot echnology “ Advancing life sciences for healt h (ant i aging development in part icular) and food securit y”

TABLE OF CONTENT
Biru: fullpaper sudah masuk layout
Hijau: hanya abstrak, sudah masuk layout
ORAL PRESENTATION: FOOD SCIENCE AND TECHNOLOGY
Code
O-1-01

Name
TRIARTI
SARASWATI
O-1-02
NI WAYAN ARYA
(permintaan UTAMI
ybs)
O-1-03
NANI PASARIBU
O-1-05
IDA BAGUS PUTU
GUNADNYA

O-1-06

NI PUTU EKA
LELIQIA

Title
Human Factors Consideration in Food Industry to Minimize Health Problems as Impact of Human-machine Interaction
Feeding Yacon Tuber-containing Oligofructose Increases Intestinal Fermentation and Alters Microbial Community Structure in Rats

THE STUDY OF STABILITY OF ANTIOXIDANT ACTIVITY IN BUTTERFLY PEA (CLITOREA TERNATEA) EXTRACT SYRUP
Effect of Initial Concentration of CO2 and Storage Temperature on the Characteristic of Packed Broccoli

CHARACTERISTICS OF MICROORGANISMS IN BLACK TEA BROTH (KOMBUCHA) IN BALI

ORAL PRESENTATION: BIODIVERSITY AND ENVIRONMENT
Code
O-2-01

Name
Iryanti Eka Suprihatin


Title
Reducing water hardness using extract of ketapang (tropical betel nut) leaves

O-2-02

I WAYAN KARTA

Preliminary study on the production of Bioethanol using Codium Geppiorum as the carbon source

O-2-03

KHALIDA KHAN

Socio-ecological Landscape Change as a Preamble to Urban Watershed Rejuvenation, Kanshi of the Jhelum River Basin, Potohar Plateau,
Pakistan

ORAL PRESENTATION: HEALTH
Code
O-3-01


Name
NANI RAHAYU
MOHD RADZUAN
O-3-02
ISSABELLINA D.
(permintaan TAMPUBOLON,
ybs)
O-3-03
SYAHIDA AHMAD
O-3-04

O-3-05
O-3-06
O-3-07
O-3-08
O-3-09
O-3-10
confirmd
O-3-11

Permintaan
ybs
O-3-12

Title
Fertility Enhancing Effects of Gynura procumbens Methanolic Extract on Diabetic Induced Male Rats
Detection of Jembrana Disease Virus Amplicon by Lateral Flow (Immuno) Assay

Anti-Neuroinflammatory Effects of Cardamonin, a Chalcone Derivative Isolated from Alpinia rafflesiana

I WAYAN
In Vivo Antimalarial Activity of Ethanolic Extract of
DWISADA
Spondias pinnata (L.f) Kurz. Leaves
PURNAMAYADI
AFIONO AGUNG
Occult Hepatitis B Detected in Human Immunodeficiency Virus Patients in Moewardi General Hospital Surakarta
PRASETYO
RASMAYA NIRURI A Pilot Project in Monitoring Serum Concentration Following Administration Amikacin Once Daily Dosing
in Electrical Burn Patients at Sutomo Hospital

NARENDRA YOGA Study Protocol: Development Multiplex Dipstick for Early Detection Human Immunodeficiency Virus -1 and Hepatitis B Virus Based on
HENDARTA
Combination of Multiplex Loop-Mediated Isothermal Amplification and Lateral Flow Dipstick
IRDHAWATI
Voltammetric Behavior of the Transfer of Methyl Ephedrine Ion across the Water|Nitrobenzene Interface
NI PUTU
ARIANTARI
RINI NOVIYANI
I MADE JAWI

FATMA

Phytochemical Analysis and In Vitro Antimalarial Activity of Ethanolic Extract of Spondias pinnata (L.f) Kurz. Leaves
Study on Prescribing and Antibiotic Dosage Accuracy in Ear, Nose and Throat Cases Prescriptions
(Case Studies in Seven Pharmacies in Denpasar City Period January – June 2010)
AQUEOUS EXTRACT OF BALINESE PURPLE SWEET POTATO
(Ipomoea batatas L.) PREVENTS OXIDATIVE STRESS AND DECREASES BLOOD INTERLEUKIN-1 IN
HYPERCHOLESTEROLEMIC RABBITS
Preliminary Study of Nutrition Household Consumption Monitoring in Yogyakarta


O-3-13
O-3-14
O-3-15
Confirmd

ZUHROTUN NISA
TENRI ASHARI
A Comparative Study on Jembrana Disease Virus Detection Methods
WANAHARI
KETUT SRI MARHAENI Extract Ethanol Seaweeds Lowering Level of Serum HMG-CoA Reductase Enzyme of Wistar Rats Hypercholesterolemia
JULYASIH
BIFIDOGENIC NATURE OF INDIGENOUS BALINESE ROOT CROPS
I N. Sujaya

ORAL PRESENTATION: AGRICULTURE

Code
O-4-01

Name

Title
BAMBANGRAHAYU The Use of Chitino-Proteolytic Actinomycetes As Biological Control Agens For Root-Knot Nematodes Meloidogyne sp.
TRISNO
PUJIASTOMO

O-4-04

RINDANG DWIYANI Micropropagation of Vanda tricolor Lindl. var. suavis forma Bali Carrying
KNOTTED1- LIKE Arabidopsis thaliana (KNAT1) Gene

O-4-06

MADE
PHARMAWATI

Ethyl Methanesulfonate Delayed Germination and Altered Seedling Morphology of Capsicum annuum L.

O-4-08
Yan Ramona
Confirmd

O-4-09
BUDI PRAKOSO

IN VITRO ANTAGONISM OF BACTERIAL BIOCONTROL AGENTS ON SEVERAL FUNGAL PLANT PATHOGENS

O-4-10

Detection of toxR, tdh and trh Genes of Vibrio parahaemolyticus Obtained from White Shrimp (Penaeus indicus) Samples in Jambi,
Indonesian Coastal Area

MARLINA

The Effect of Seed Treatment with Liquid Product of Shrimp Waste Fermentation on Chili

POSTER PRESENTATION: FOOD SCIENCE AND TECHNOLOGY

No
P-1-01

Name

NI W. WISANIYASA

P-1-02
P-1-03
confirm
d
P-1-04
(confirm
ed ybs)
P-1-05
P-1-06
confirmd
P-1-07

DIAH PUSPAWATI
Ida Ayu Rina Pratiwi
Pudja

Title
THE INFLUENCE OF SOLVENT TYPES AND pH ON THE ANTIOXIDANT ACTIVITY OF ANTHOCYANIN OF FLESH RED

DRAGON FRUIT (Hylocereus costaricensis) EXTRACT
The Change of Antioxidant Activity of Tamarillo Fruit (Chyphomandra betacea S) after Processing Into Sauce
CHANGES OF TEXTURE AND RESPIRATION RATE OF SALAK FRUIT IN MODIFIED ATMOSPHERE USING
POLYETHYLINE PLASTIC PACKAGING AT VARIOUS PERFORATION

SRI MULYANI

The Effect of Ratio Tamarind Pulp and Tamarind Leaves (Tamarindus indica L) and Cooking Time on The Antioxidant Activities

TIMUR INA
I KETUT SUTER1

Effect of CMC Concentration on Characteristic of Pineapple (Ananas comosus L.Merr) Juice
Study on Storage Method of Instant Ledok

NI M. YUSA

The Influence of Solvent Type and Ratio of Material With Solvent on Antioxidant Activity of Pulp Tamarillo Ekstract (Chyphomandra
betacea Sendth).


BAMBANG ADMADI
HARSOJUWONO
YAYA RUKAYADI*

Determination of Potato Sweet Varietes As Diet Food Of Diabetes Mellitus Patients

P-1-8

P-1-9
confirmd
P-1-10
YAYA RUKAYADI1
confirmd
P-1-11
NI NYOMAN
PUSPAWATI
P-1-12
GUSTI AYU EKAWATI1

Antibacterial Activity of Fermented Pangium edule Reinw. Seeds Extract against Foodborne Pathogens
Shelf-Life Stability of Milk Chocolate Containing Inulin
Survival of freeze-dried Lactobacillus rhamnosus R21 in the presence skim milk as protectant during storage
Extraction of Natural Colorant From Red Cabbage (Brassica oleracea var. capitata l. f. rubra (l.) thell) By Using Tartaric Acid

AGUNG ANAK MADE
DEWI ANGGRENI
WIDNYANA YOGA

The Usage Of Beans Flour As Protein Source In Wet Noodle Processing

P-1-15

Putu Ari Sandhi
Wipradnyadewi

THE APPLICATION OF Rhizopus oligosporus MK 1 TO REDUCE AFB1 CONTAMINATION IN TEMPEH FERMENTATION

P-1-16

NI KADEK

Comparing Extraction Methods To Isolate Andrographolide From The Bitter Herb (Andrographis paniculata Burm.f) Ness)

P-1-13
P-1-14

Identification of Gel Forming Component (GFC) of Kacapiring Leaf ( Gardenia jasminoides Ellis)

WARDITIANI
TG Belawa Yadnya
Wayan Rai Widarta
Siti Helmiyati

The Usage of Purple Sweet Potato (Ipomoea batatas) In Ration Fermented by Aspergillus niger For Improving Feed Conversion
and Blood Lipid Profile of Bali Duck
Extraction of Bioactive Compounds and Antioxidant Activity of The Local White, Red, and Black Rice Bran Extracts by Various
Solvents
Study Protocol: Effect of Iron Fortification of Fermented Milk with Local Probiotic Lactobacillus plantarum Dad 13 and Prebiotic
Fructo Oligosaccharide on the Iron Content and Total Lactic Acid Bacteria During Storage

POSTER PRESENTATION: BIODIVERSITY AND ENVIRONMENT
Code
P-2-01

Name
NI LUH SURIANI

Title
HEAVY METAL BIOREMEDIATION LEAD (Pb) TO AGRICULTURAL LAND ON THE EDGE OF LAKE BUYAN BALI WITH
PLANT Sansevieria lorentii

P-2-02
Confirm
d
P-2-03
confirmd
P-2-04
confirmd
P-2-05

ENIEK KRISWIYANTI,

Alleles Variation of Coconut Accessions (Cocos nucifera L., Arecaceae) Based on Microsatellite DNA.

I KETUT JUNITHA

Genetic Diversity of Soroh Celagi (Pasek Catur Sanak Clan) Based on Y-Chromosomal Microsatelites DNA
,
Study of Socio-engineering the Subak System Development with Agroecotourism Oriented

P-2-06
confirmd
P-2-07
confirmd
P-2-08

SUMIYATI
I WAYAN SUSILA

The Identification and Investigation of Parasitization Rate of Parasitoids of Hairy Caterpillar Lymantria marginata Wlk. (Lepidoptera:
Lymantriidae) in Buleleng Bali
Biocatalytic desulfurization of dibenzothiophene by Pseudomonas sp. strain KWN5

IDA BAGUS WAYAN
GUNAM
IDA AYU ASTARINI

Induce Poliploidy of Lilium longiflorum Thunb. using Oryzalin Treatment

Ni Made Indri Hapsari

Composting Acceleration of Kitchen W aste Using M icrobial Inoculant

POSTER PRESENTATION: HEALTH

Code
Name
P-3-01
Darukutni
P-3-02
DEWA NYOMAN OKA
confirmd
P-3-3
NI GUSTI AYU
confirmd MANIK
ERMAYANTI
P-3-4
I GUSTI AYU SRI
confirmd MAHENDRA DEWI
P-3-5
YULIA SARI
confirmd
P-3-6
RUBEN DHARMAWAN
confirmd
P-3-7
SUYATMI
confirmd
P-3-8
MARWOTO
confirmd
P-3-9
LELI SAPTAWATI
confirmd
P-3-10
HUDIYONO
confirmd
P-3-11
NI PUTU
ARYADNYANI
P-3-12
NI PUTU SRIWIDYANI

TITLE
Use of Glucose Determination for Candida albicans β Glucans Examination
THE IMPLEMENTATION OF MODULE-JIGSAW COOPERATIVE LEARNING STRATEGY, IMPROVING THE PREVENTIVE
BEHAVIOR TOWARD DENGUE HEMORRHAGIC FEVER
The Effect of Amargo Wood (Quassia amara Linn.) Infuse on the Mice (Mus musculus L.) Testical Weight and Its Recovery

Classic Hodgkin’s Lymphoma Simulating Anaplastic Large Cell Lymphoma
Molecular Analysis of HBV Precore/Core Region of Hepatitis B Virus Isolated from Men Who Have Sex With Men in Surakarta
Fungal Infection in Human Immunodeficiency Virus Patients in Moewardi General Hospital Surakarta
Apoptin Expression Plasmid For Expression of Apoptin in Mammalian Cells
Mycobacterium Tuberculosis in Human Immunodeficiency Virus Patients’ Blood in Moewardi General Hospital Surakarta
Mycobacterium Tuberculosis Complex (MTC) and Nontuberculous Mycobacteria (NTM) in Human Immunodeficiency Virus Patients’
Blood in Moewardi General Hospital Surakarta
Molecular Analysis of HBV Pre-S/S Region of Hepatitis B Virus Isolated from Men Who Have Sex With Men in Surakarta
Extending Time of Kombucha Tea Fermentation Improves The Growth of Impediment Capasity of Bacteria Escherichia Coli ; The
Producer of Extended Spectrum Beta Lactamases (ESBL) by In Vitro Method
ROLE OF P53 IN CELLULAR AGING

P-3-13
MADE AGUS
confirmd HENDRAYANA

Identification of Vibrio cholera’s ctxA Gene by PCR Methode from Sea Water Around Bali Island

P-3-14

I MADE SUKADANA

Antibacterial Terpenoid Compounds from Rhizome of Curcuma zedoaria (Berg). Roscoe

P-3-15

NiMade Rai Suarni

CIGARETE SMOKE EXPOSURE DECREASE SPERM QUALITY OF MALE MICE (MUS MUSCULUS L.)

P-3-16
ANAK AGUNG AYU
confirmd MIRAH ADI
P-3-17
(confirm
)
P-3-18
confirmd
P-3-19
confirmd
P-3-20
P-3-21
confirmd
P-3-22
confirmd
Confirm
d
Confirm
ed
Confirm
d

P-3-xx
Confirm
d

Amplifications of Terminal Ends of Newcastle Disease Virus Genome by Rapid Amplification of Complementary Deoxyribonucleic Acid
Ends

I NYOMAN SUARSANA Antioxidant Enzyme Activities In Various Tissue On Rat Under Hyperglycemia Condition

I G K NYOMAN
Epigenetic Approach as Potential Anti-Aging Therapy of Andropause in the Future
ARIJANA
I WAYAN SUMARDIKA Antioxidative And Nephroprotective Activity Of Balinese Purple Sweet Potato
(Ipomoea batatas L.) In Diabetic Rats
KOMANG ARDI
TREATMENT OF TOPICAL ASTAXANTHIN GIVE A PROTECTIVE EFFECT ON COLLAGEN IN PREMATURE SKIN AGING
WAHYUNINGSIH
INDUCED BY UV B IRRADIATION IN MICE (Mus musculus)
IBN Putra Dwija
Human Papilloma Virus Genotyping from Archive of Paraffin Block of Patient with CIN Diagnose
K.A. Nocianitri1

Development of specific PCR-RAPD Method for detection of Lactobacillus sp F2

Ni Made Linawati

The Role of Interleukin 22 in Lung Tuberculosis and Its Molecular Aspects

IGA Dewi Ratnayanti

THE ROLE OF p16INK4a IN CELLULAR SENESCENCE

IA Wahyuniari

THE EXTRACTS OF CACAO BEANS (Theobroma cacao L.) INCREASE BLOOD SOD CONCENTRATION IN OXIDATIVE
STRESS WHITE RAT (Rattus norvegicus)

Paramasari Dirgahayu

Coinfection of Toxoplasma gondii among HIV/HCV infected and uninfected people in Correctional
facility Indonesia
The Study of Bcl-2 Expression of Red Fruit Oil Ethanol Extract on Breast Cancer Cell Line T47D

Agung Wiwiek Indrayani
Ni Made Adi Tarini
Sri Budiarti
Made Sudarjana

DUPLEX PCR IS RAPID METHOD FOR DETECTION Legionella pneumophila and Legionella sp ON ENVIRONMENTAL WATER IN

Isolation and Characterization lytic Phage of Salmonella sp. FR38.
ADMINISTRATION OF ORAL ALPHA-LIPOIC ACID INHIBITS THE REDUCTION OF SKIN DERMAL COLLAGEN
EXPRESSION INDUCED BY UVB IRRADIATION IN BALB/C MICE

P-3-31
I Nyoman Wande
confirmd

Role Of TLR2 On Mycobacterium Tuberculosis Infection

P-3-xx
confirmd

N.W.Bogoriani

SAPONINS APPLICATION AS ANTI CHOLESTEROL

POSTER PRESENTATION: AGRICULTURE
Code
P-4-1
confirmd
P-4-2
confirmd
P-4-3
P-4-4

Name
I WAYAN ARNATA

TITLE
Production of Crude Enzyme Cellulases from Cassava Waste by Trichoderma viride

NI MADE WARTINI

P-4-5
confirmd
P-4-6
Confirm
d
P-4-7
Confirm
d
P-4-8

MADE RIA DEFIANI

THE INFLUENCE OF CURING TIME AND EXTRACTION TIMES ON THE YIELD AND CHARACTERISTICS ESSENTIAL OIL
OF SANDALWOOD FRANGIPANI FLOWERS (Plumeria alba).
Potential Of Plant Extracts As Growth Regulators: The Influence of Plant Extract to Growth of Cutting of Potato Seeding.
Detection of Potato Leaf Roll Virus (PLRV), Potato Virus Y (PVY) and Potato Virus X (PVX) on Five Potato Varieties by Using of
DAS-ELISA and RT-PCR Methods
Media Composition Improved The Growth of New Leaves in Anthurium Plants

I G. L. O. CAKRA

Urea-Lime Mixture as a Rumen Slow-Release Urea on In Vitro Rumen Fermentation and Microbial Protein Synthesis

IDA AYU RINA
PRATIWI PUDJA

Technique " Pulsing" With Addition Of Sucrose And Antimicrobial To Change Of Texture and Freshness of Roses During Storage

FACHIRAH ULFA
TUTIK KUSWINANTI,

YU JIN JUNG , KYUNG
HEE HAN, HEE WAN
KANG , ILL SUP NOU,
KWON KYOO KANG
P-4-9
JONG IN PARK, HEE
JEONG JUNG, HEE
WAN KANG, KWON
KYOO KANG,
ILL SUP NOU
P-4-10
G. A. M. KRISTINA
confirmd DEWI

Soil Improvement Effects of Four Green Manure Crops
in Greenhouse Cultivation

Effect of Green Manure Crop Extracts on Potato Common Scab (Streptomyces scabiei)

THE EFFECTS OF DIFFERENT ENERGY - PROTEIN RATION FOR CARCASS OF KAMPUNG CHICKENS

P-4-11
P-4-12
P-4-13

P-4-14

I DEWA MADE PUTRA
WIRATAMA1
GUSTI NGURAH GEDE
DHARMA PUTRA1
I MADE SUKEWIJAYA

Monitoring of Imago of Spodoptera litura and Helicoverpa armigera
Using Sex Pheromone Trap
The Misting Period Application on Postharvest Quality of Oyster Mushrooms (Pleurotus ostreatus) in Low Land Cultivation

Made
Sritamin

Confirmation (Citrus Vein Phloem Degeneration) CVPD Infection Toward Orange Plants Varieties with PCR Technique
and Total Protein Profile

The Variety Trial of Resistant Chili Peppers to Anthracnose

P-4-15
NI MADE INDRI
confirmd HAPSARI
ARIHANTANA1

Composting Acceleration of Kitchen WasteUsing Microbial Inoculant

P-4-16
Ketut Ayu Yuliadhi
confirmd

The Parasitism Level of Parasitoid Larvae Diadegma semiclausum Hellen (Hymenoptera:Ichneumonidae) on Plutella xylostella
(Lepidoptera: Plutellidae) in Candikuning Village, Tabanan Regency, Bali, Indonesia

P-4-17

HILDA KARIM

P-4-18

Ni Luh Yulianti

SCREENING AND CHARACTERIZATION OF MICROBIAL ANTAGONISTS FROM RHIZOSPHERE OF PASSION FRUITS
(PASSIFLORA SPP) AS BIOCONTROL AGENTS OF FUSARIUM WILT
DESIGN OF THE FLUIDIZED BED DRYER FOR CHILI SEED

P-4-19
SUTARPA , I N.S1
Confirm
ed
P-4-xx
IGA Arta Putra
P-4-25
I. G. N. G. BIDURA
confirmd
P-4-26
I GEDE RAI MAYA
confirmd TEMAJA
P-4-27

Kumala Dewi

O-4-02

BAHARUDDIN

The Effect of Waste From Purple Sweet Potato Syrup Production on the Performance, Lipid Blood Content, and Total Bacteria
of the Digestive Tract of Kampong Chickens
The Evaluation of Offering Cortisone Hormone and Ration Supplemented by Papaya (Carica papaya) Leaf Meal to the
Performance of Duck on Growth Phase
THE IMPLEMENTATION OF Saccharomyces Spp.N-2 CULTURE (ISOLATION FROM TRADITIONAL YEAST CULTURE) FOR
DECREASING ABDOMINAL FAT AND SERUM CHOLESTEROL CONTENTS OF BALI DUCKLING
First Report on Occurrence of Grapevine Leaf Roll-associated Virus Type 3 on Grapevine in Indonesia

The effect of Glucose and Light Quality on Vitamine E and
Chlorophyll content of Green Bean Sprout (Vigna radiata (L.) Wilcz.)
Efectiveness of Clostridium Isolates on Aeroponically Cultivated Potato to Protect Against Ralstonia solanacearum (Bacterial Wilt) and

PCR Detection on
Laten Tuber Infections

CHANGES OF TEXTURE AND RESPIRATION RATE OF ZALACCA FRUIT IN MODIFIED
ATMOSPHERE USING POLYETHYLINE PLASTIC PACKAGING AT VARIOUS PERFORATION

Ida Ayu Rina Pratiwi Pudja
Department of Agriculture Engineering, Faculty of Agricultural Technology, Udayana University
Email: dayu_rina@yahoo.co.id

ABSTRACT

The aim of this reaserch were to study changes of texture of Zalacca fruits from consequence of
modified atmosphere packaging and to study of respiration rate of Zalacca fruit to preserve at modified
atmosphere packaging.
The treatment of this reasearch was packaging with polyethyline plastic package or passive atmosphere
with thickness 0.02 mm and perforation diameter hole is 0.6 cm. The various perforation is 0, 4, 8 and 12 hole.
The storage time was set at 0, 2, 4, 6, 8, 10, 12 day in room temperature.
The result showed that the changes of texture of Zalacca fruit was increased during the storage time. The
changes of texture rate was slowly at 8 hole polyethyline plastic perforation packaging. At the begining of the
storage time, the respiration rate have been increased while in the midle have been decreased. Finally, at the
end, the storage time have been increased. The respiration rate was slowly at 8 and 12 hole polyethyline plastic
perforation packaging.

Keywords :

Modified Atmosphere, Polyethyline plastic perforation packaging,
Zalacca Fruit

INTRODUCTION

There is inclination that consumer be apt to food product which are fresh, practical and high quality
(Daugherty, 1990; Labuza and Breene, 1989; Myers, 1989). In last decade in the U.S.A., it was noted that
consumption of fresh food product increase in 12%, frozen food product increase by 10%, but canning food
product decrease by 10%. Therefore, Indonesia has good opportunity of exporting fruits and vegetables. In
order to increase the export value of agro-industry, fruits and vegetables should be cared and handled very well.
There is a problem that zalacca fruit is perishable and have short of time keeping. Senescence will
occur after maturation and continued with damage due to the decrease of microbe defenses. Damage is usually
caused by mechanical, physical, microbe, and physiological. Mechanical damage is frequently caused by chafe,

peeled off, and bruised. Microbe damage is caused by infection and microbe activity. Physiological damage
occurs due to internal metabolism reaction resulting in putrefaction.
Zalacca (Salacca edulis, Reinw) is Indonesia native fruit. Production was high, reaching 662,546 tons
in 1995 (Anon, 1995 in Agung et. al., 1999). The cultivation areas of zalacca in Indonesia are Bandung
(Batujajar), Tasikmalaya (Manonjaya), Jogjakarta (Sleman), Malang, North Sumatera, South Sulawesi, Central
Java (Ambarawa and Magelang) and Bali.
The increasing production and fulfillment of demand should be balanced with post harvest treatment.
This should be considered becausezalacca as other fruits is easy to get damage. The negative environment
factors along the storage cause of fast decreasing quality resulting in short storage time.
In order to increase the storage time and to avoid damage, delaying the mature is performed by
decreasing oxygen absorption and release of CO2 of zalacca. This can be done with atmosphere modification.
Low oxygen and high CO2 in modified atmosphere give influence in decreasing respiration rate, ethylene
production rate, retard the maturation and others relate to mature such as soften of fruit, change of color, lose of
sugar and retard the putrefaction (Brecht, 1980; Kader, 1980).
An appropriate post-harvest treatment of zalaccamay give result in added value of long storage time
which means more profit for the farmer, seller and other parties.

AIMS

The aims of the research are
a. Studying changes of texture of fruit as result of storage in modified atmosphere.
b. Studying respiration speed of zalacca which are stored in modified atmosphere as function of O2.
METHODS
The research steps are preparation, respiration speed quantification, changes of the texture of fruit as
result of storage in modified atmosphere, descriptive and graphical analyze.
A. Treatments
The treatments are polyethylene plastic cover with thickness of 0.6 cm (positive modified atmosphere)
and storage time. The perforation of polyethylene plastic cover are 0 hole (without perforation), 4 holes, 12
holes and the storage time are 0, 2, 4, 6, 8, 10, 12 days. The samples treatment and one which is uncovered and
used as the control group are stored in room temperature.

B. Respiration rates
a. In tight close container
Close system is used in this experiment (Hangar et al., 1992 and Lee, 1997 in Song et al., 1992). Three
samples are put in glass container/jarwith volume of 2.126 liters. Glass container is closed tightly (with
glue/paint/vaseline to avoid leak).
In the middle of the lid, a hole cover with rubber is set to take the gas sample. This container is put in
room temperature. Gas samples are collected every 2 days in 12 days. O2 and CO2 concentration are
detected with cosmotector. Type XP-314 cosmotector is used for O2 and XP318 for CO2. The relieved
volume in the container is also counted. These treatments are repeated for three times.

b. In polyethylene plastic
Three samples are put in a sealed 6.80 liters volume of polyethylene plastic. A hole cover with rubber
is set in the bottom part of the polyethylene plastic to make easy for taking the gas sample. The edge of the
hole is sealed with glue/paint/vaseline to avoid leak. And then is put in room temperature. Gas samples are
collected every 2 days in 12 days. O2 and CO2 concentration are detected with cosmotector. Type XP-314
cosmotector is used for O2 and XP318 for CO2. The relieved volume in the container is also counted. These
treatments are repeated for three times.

c. Change of Texture
The probe with diameter of 0.52 mm of FT 327 model of Hand Penetrometer Effegi is used to test
physically the stringent of Bali’s zalacca to get data of texture of Bali’s zalacca. A small area of skin of
sample was peeled and then holds by one hand while the other hand pushed the sample with Hand
Penetrometer Effegi. The number in kg/cm2 read from the tool shows the texture value of the fruit. The test
was repeated for four times for each sample and it was done for some samples. The values were then
calculated for the average number.

RESULT AND DISCUSSION

1. Changes of Texture
Texture is a decisive factor of fresh fruits. Changes of texture of Bali’s zalacca showed that there was
a variation of texture of Bali’s zalacca in storage. The graph of texture changes can be seen in Figure 1.
7.5
7
6.5
Texture (kg/cm 2)

6
5.5
5
0

2

4

6

8

10

12

14

Storage time (day)
Kontrol

P0

P4

P8

P12

Fig 1. The changes of texture of Bali zalacca fruits during the storage time

Figure 1 showed that the changes of texture level of control of Bali’s zalacca and all the Bali’s zalacca
treated with different perforation of polyethylene plastic container decrease during the storage. The decrease
intextureis caused by saturated decomposing propectin to pectin enzyme activity. The solid state rate is
caused by the change of cell size and turgor. The reason of texture become lossis related with high level of
respiration activity and transpiration of the fruit causing loss of liquid. Therefore, cell size and pressure of
inside cell to the cell wall decrease (Suter, 1988).

2. Respiration Rate
The data of O2 respiration rate of Bali’s zalacca in storage is presented in Figure 2. Figure 2 showed
that in every treatment, respiration rate of O2 changed rapidly, then were going slowly, and finally in a
certain time changed rapidly until the last period of storage.
Figure 2shows that O2 respiration rates were increase in the beginning of storage, then decrease, and
increase again till the last period of storage. Decrease of respiration rate is caused by a decrease in the
substrate for respiration. As addition, the O2 in the container is used by the fruits for respiration and
substrate oxidation. With limitation of O2 in the container causing the delay of chlorophyll degradation, low
production of C2H4, decrease in production rate of ascorbic acid, change in composition of unsaturated fat
and degradation of pectin were not fast as in nature. These things were reflected as the delay of mature of
fruit and, therefore, storage capacity of the fruit is long (Amiarsi et al., 1996).
0,03
0,025
0,02
0,015
Respiration rate of
0,01
O2 (ml/jam/g)
0,005
0
-0,005 0

2

4

6

8

10

12

14

-0,01
Storage time (day)

Kontrol

P0

P4

P8

P12

Fig 2. O2 Respiration rate of Balizalacca fruits during the storage time

CONCLUSION
 The weight of Balizalacca fruit was decreased during the storage time.
 At the beginning of storage time, the result showed that the respiration rate of O2 have been increased
while in the middle have been decreased.
 We can use the polyethylene plastic packaging at perforation with 12 holes in modified atmosphere.
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