2. Price Rf can be analyzed to determine if the compound has a standard compound, comparing the price of a standard compound with Rf compound being analyzed. If
it is found in the literature that analyzed the compound Rf prices it can also for comparison of compounds in accordance with the analysis of compounds having Rf
rates in the literature. 3. TLC analysis to determine the pure compound can be a way to do anaalisis
adsorbennya TLC one spot and then scraped off and subsequently dissolved in the same eluaen. After the eluent is evaporated it will get a pure compound.
IMPLEMENTATION PLAN FOR LEARNING
MEETING TO VI SOXHLET EXTRACTION
ISOLATION OF SEEDS TRIMIRISTIN PALA
Faculty Study Program : Faculty of Mathematics and Natural Sciences Chemistry
Courses Code : Practical Organic Chemistry II
Number of credits : 1 sks
Semester and Time : 4 dan 2 x 50 xmenit
I Standard Competency::
Understanding the properties of the eluent, adsoeben and analysis of thin-layer chromatography TLC
:II. Basic competence
Predicting the appropriate use of the eluent to separate the organic compounds
III. Indicator
1. Explaining the use of pure eluent and the eluent mixture 2. Rf determine the price of a particular organic compound
IV. Learning objectives
1. Can determine the proper eluent for TLC analysis 2. Students can determine which compounds were analyzed based on the price of Rf
obtained from TLC analysis..
3. Students can purify the compound by TLC analysis V. Learning Materials
A common way used for the separation of a mixture of organic compounds obtained from the reaction is the extraction of the liquid with liquid. The way the separation is based on
comparison of the distribution of substances samples are dissolved in two solvents are not mutually dissolved. Comparison of solute in two solvents in the process of partitioning is
called the distribution constant K, the balance at a specific temperature and state of the substance dissolved in a solution of the same substance and the distribution of solute A in
solution 1 and solution 2 is formulated as follows:
K = C1
C2 =
konsentrasi zat A gl dlm pelarut 1 konsentrasi zat A gl dlm pelarut 2
If the weight of the solute = CE extracted using a solution whose volume is equal to the volume palarut and C0 is the weight of the substance before it is extracted:
C
E
= C KK + 1
So for example, 2 parts of the solute can be dissolved in solvent ether and water and if the partition coefficient = 2 or 67 can be extracted using ether solvent then :
K = C
eter
C
H2O
= 21 = 2 C eter = 100 2 + 2 + 1 = 67
In the second extraction extraction residue extracted suppose there is 22 and the third extraction extraction residue is extracted a second there are 4 of the solute which can be
seen in the ether layer : C
2 eter
= 100 – 67 23 = 22 C
3 eter
= 100 – 67 – 22 23 = 7 C
H2O
sesudah ekstraksi = 100 – 67 – 22 – 7 = 4 Extraction can be divided into cold extraction solvent extraction and heat extraction
Soxhlet extraction. Cold extraction performed unntuk liquid - liquid substances which are not mutually mixed and used in separating funnel. In this case must be considered agitation
should be homogeneous. Heat extraction done to attract organic compounds contained in natural materials such as roots, stems, leaves, flowers and seeds, this extraction using Soxhlet.
Organic compounds contained in natural materials such as trimiristin contained in nutmeg. Trimiristin is a triglyceride ester which is pretty much contained in nutmeg miristica
fragrans. Trimiristin molecular formula is C45H86O6. If it will happen trimiristin hydrolyzed myristic acid and glycerol. Hydrolysis reaction can take place in an atmosphere of sam or
alkali. For Soxhlet extraction trimiristin used tools, nutmeg mashed and then wrapped using fat-free
filter paper. Then inserted into a Soxhlet device. Tools associated with the Soxhlet flask filled with a solvent as much as 2 3 volume of the flask and then heated. At the time of heating the
solvent will boil and steam will rise to the cooler and then steam mmengembun and will fall into the sample and Soxhlet meet trimiristin will dissolve. After the solvent memenuhia solvent will
descend into the Soxhlet flask .. One round is called a circulating solvent. In general, extraction done as much, 8, 10, 12, 14 or 16 circulating solvent.
VI. Learning methods
Methods: Practical Approach : -
Strategy: Learning-based life skill
VII. Learning Steps No
Kegiatan Waktu
1. Preliminary
a. opening 1. Lecturer in greeting open class
2. Lecturer check student attendance 3. Collect lab reports from previous
b. apperception 1. Lecturer gives an example of installing a practicum
2. problem Why is the coolant used Soxhlet extraction ball?.
5 minutes
2. Core Activities
lecturer: a. Inform briefly about the TLC analysis
b. Divide the class into 10 groups 90 minutes
students: a. Noting the information from the lecturer
b. Conducting experiments
3. Cover
Message: do problems that exist in the user guide appropriate practicum practicum each event and make
practical reports 5 minutes
VIII. Tools and Learning Resources
Worksheets experiments, equipment and lab materials, manuals Organic Chemistry II lab
IX. Appraisal
Assessment Sheet for Internship Experiments in the laboratory No
Student name The presence in
conducting Activeness in
conducting Cooperation in
carrying out activities
1. 2.
3. 4.
X. Solved
1. Type of bill: the task of practical reports 2. Istrumen shape: a matter of description, spreadsheet
3. about 1. Mention solvents other than hexane can be used for the isolation trimirisin
2. Soxhlet extraction for the separation of organic compounds from natural materials, whether daasr determination of the solvent.
4. Answer : 1. Solvents other than hexane can be used for isolation trimirisin are polar solvents that
have approached the polar hexane. Since then the hexane is non polar solvents have been used also must be non-polar. Example is the solvent n-pentane, and cyclohexane
isooktana.
2. he basis for the separation of organic senhyawa ekstraaksi natural materials are polar compounds derived from natural materials and the polar solvent used.
IMPLEMENTATION PLAN FOR LEARNING
MEETING TO VII ALDOL CONDENSATION CROSS
Faculty Study Program : Faculty of Mathematics and Natural Sciences Chemistry
Courses Code : Practical Organic Chemistry II
Number of credits : 1 sks
Semester and Time : 4 dan 2 x 50 xmenit
I. Standard Competency: